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The map label for this gene is lipA

Identifier: 50812289

GI number: 50812289

Start: 3320324

End: 3321220

Strand: Reverse

Name: lipA

Synonym: BSU32330

Alternate gene names: 50812289

Gene position: 3321220-3320324 (Counterclockwise)

Preceding gene: 16080288

Following gene: 255767741

Centisome position: 78.78

GC content: 46.71

Gene sequence:

>897_bases
TTGGCAAAGAAAGACGAACACCTCAGAAAGCCCGAATGGCTTAAAATTAAATTAAATACAAATGAAAACTATACAGGGCT
CAAAAAACTCATGCGGGAAAACAACCTGCATACCGTATGCGAAGAAGCAAAGTGTCCGAATATTCATGAATGCTGGGCGG
TCAGACGTACAGCGACATTTATGATTTTAGGTTCTGTTTGTACAAGAGCATGCCGTTTCTGTGCTGTCAAAACAGGCCTT
CCAACTGAGCTTGATCTGCAAGAGCCGGAACGCGTAGCGGATTCAGTCGCGCTTATGAATTTGAAACACGCGGTCATTAC
GGCTGTTGCGCGTGACGATCAGAAAGATGGCGGGGCAGGCATTTTCGCGGAAACTGTCCGTGCGATCCGCAGAAAAAGCC
CGTTCACAACAATTGAGGTGCTGCCGTCTGATATGGGCGGAAACTACGATAACCTAAAAACCTTGATGGATACACGCCCA
GACATCCTGAATCACAATATCGAAACGGTTCGCCGCCTGACGCCAAGAGTCCGCGCGCGTGCGACGTACGACCGTTCATT
GGAATTTTTGCGCCGTGCGAAGGAAATGCAGCCTGACATTCCGACAAAATCAAGTATCATGATCGGACTCGGCGAAACAA
AAGAAGAGATTATTGAAGTAATGGACGACCTCTTGGCGAACAACGTGGATATTATGGCAATCGGCCAATATCTGCAGCCG
ACGAAGAAACACTTGAAAGTCCAAAAATACTACCATCCTGATGAATTTGCAGAATTAAAAGAAATCGCGATGCAAAAAGG
CTTCAGCCATTGTGAAGCCGGTCCGCTTGTCCGTTCCTCTTACCATGCGGATGAACAAGTAAACGAAGCATCTAAAAAGC
GTCAAGCACAAGCATAA

Upstream 100 bases:

>100_bases
AAACATTATTTTTGTTGCAGAACTAAAACCTGCACCTTTCTCGTTTTCAGAATGTTTCGTGTCAGAAATTGAGTATATTG
GATATTGGAGATGATGGGAA

Downstream 100 bases:

>100_bases
TGCCAAAACGCCAGATCATCTGGCGTTTTTGTCATTCCTATCGATTTTCATGTCCTGACGCGTTTCTCCATTGGCATTTT
CATTCTCTGAGACAGGGCTT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MAKKDEHLRKPEWLKIKLNTNENYTGLKKLMRENNLHTVCEEAKCPNIHECWAVRRTATFMILGSVCTRACRFCAVKTGL
PTELDLQEPERVADSVALMNLKHAVITAVARDDQKDGGAGIFAETVRAIRRKSPFTTIEVLPSDMGGNYDNLKTLMDTRP
DILNHNIETVRRLTPRVRARATYDRSLEFLRRAKEMQPDIPTKSSIMIGLGETKEEIIEVMDDLLANNVDIMAIGQYLQP
TKKHLKVQKYYHPDEFAELKEIAMQKGFSHCEAGPLVRSSYHADEQVNEASKKRQAQA

Sequences:

>Translated_298_residues
MAKKDEHLRKPEWLKIKLNTNENYTGLKKLMRENNLHTVCEEAKCPNIHECWAVRRTATFMILGSVCTRACRFCAVKTGL
PTELDLQEPERVADSVALMNLKHAVITAVARDDQKDGGAGIFAETVRAIRRKSPFTTIEVLPSDMGGNYDNLKTLMDTRP
DILNHNIETVRRLTPRVRARATYDRSLEFLRRAKEMQPDIPTKSSIMIGLGETKEEIIEVMDDLLANNVDIMAIGQYLQP
TKKHLKVQKYYHPDEFAELKEIAMQKGFSHCEAGPLVRSSYHADEQVNEASKKRQAQA
>Mature_297_residues
AKKDEHLRKPEWLKIKLNTNENYTGLKKLMRENNLHTVCEEAKCPNIHECWAVRRTATFMILGSVCTRACRFCAVKTGLP
TELDLQEPERVADSVALMNLKHAVITAVARDDQKDGGAGIFAETVRAIRRKSPFTTIEVLPSDMGGNYDNLKTLMDTRPD
ILNHNIETVRRLTPRVRARATYDRSLEFLRRAKEMQPDIPTKSSIMIGLGETKEEIIEVMDDLLANNVDIMAIGQYLQPT
KKHLKVQKYYHPDEFAELKEIAMQKGFSHCEAGPLVRSSYHADEQVNEASKKRQAQA

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=289, Percent_Identity=44.2906574394464, Blast_Score=258, Evalue=5e-69,
Organism=Homo sapiens, GI37577164, Length=252, Percent_Identity=44.8412698412698, Blast_Score=224, Evalue=5e-59,
Organism=Escherichia coli, GI1786846, Length=281, Percent_Identity=53.7366548042705, Blast_Score=300, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI32564533, Length=294, Percent_Identity=45.578231292517, Blast_Score=246, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6324770, Length=297, Percent_Identity=46.1279461279461, Blast_Score=266, Evalue=4e-72,
Organism=Drosophila melanogaster, GI221513272, Length=302, Percent_Identity=46.6887417218543, Blast_Score=263, Evalue=1e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 33921; Mature: 33790

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKDEHLRKPEWLKIKLNTNENYTGLKKLMRENNLHTVCEEAKCPNIHECWAVRRTATF
CCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHH
MILGSVCTRACRFCAVKTGLPTELDLQEPERVADSVALMNLKHAVITAVARDDQKDGGAG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
IFAETVRAIRRKSPFTTIEVLPSDMGGNYDNLKTLMDTRPDILNHNIETVRRLTPRVRAR
HHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHH
ATYDRSLEFLRRAKEMQPDIPTKSSIMIGLGETKEEIIEVMDDLLANNVDIMAIGQYLQP
HHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEECCHHHHH
TKKHLKVQKYYHPDEFAELKEIAMQKGFSHCEAGPLVRSSYHADEQVNEASKKRQAQA
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKKDEHLRKPEWLKIKLNTNENYTGLKKLMRENNLHTVCEEAKCPNIHECWAVRRTATF
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHH
MILGSVCTRACRFCAVKTGLPTELDLQEPERVADSVALMNLKHAVITAVARDDQKDGGAG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
IFAETVRAIRRKSPFTTIEVLPSDMGGNYDNLKTLMDTRPDILNHNIETVRRLTPRVRAR
HHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHH
ATYDRSLEFLRRAKEMQPDIPTKSSIMIGLGETKEEIIEVMDDLLANNVDIMAIGQYLQP
HHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEECCHHHHH
TKKHLKVQKYYHPDEFAELKEIAMQKGFSHCEAGPLVRSSYHADEQVNEASKKRQAQA
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA