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The map label for this gene is trpB

Identifier: 50812257

GI number: 50812257

Start: 2372304

End: 2373506

Strand: Reverse

Name: trpB

Synonym: BSU22640

Alternate gene names: 50812257

Gene position: 2373506-2372304 (Counterclockwise)

Preceding gene: 255767487

Following gene: 16079320

Centisome position: 56.3

GC content: 45.3

Gene sequence:

>1203_bases
ATGTATCCATATCCGAATGAAATAGGCAGATACGGTGATTTTGGCGGAAAGTTTGTTCCGGAAACACTCATGCAGCCGTT
AGATGAAATACAAACAGCATTTAAACAAATCAAGGATGATCCCGCTTTTCGTGAAGAGTATTATAAGCTGTTAAAGGACT
ATTCCGGACGCCCGACTGCATTAACATACGCTGATCGAGTCACTGAATACTTAGGCGGCGCGAAAATCTATTTGAAACGA
GAAGATTTAAACCATACAGGTTCTCATAAAATCAATAATGCGCTAGGTCAAGCGCTGCTTGCTAAAAAAATGGGCAAAAC
GAAAATCATTGCTGAAACCGGTGCCGGCCAGCATGGTGTTGCCGCTGCAACAGTTGCAGCCAAATTCGGCTTTTCCTGTA
CTGTGTTTATGGGTGAAGAGGATGTTGCCCGCCAGTCTCTGAACGTTTTCCGCATGAAGCTTCTTGGAGCGGAGGTAGTG
CCTGTAACAAGCGGAAACGGAACATTGAAGGATGCCACAAATGAGGCGATCCGGTACTGGGTTCAGCATTGTGAGGATCA
CTTTTATATGATTGGATCAGTTGTCGGCCCGCATCCTTATCCGCAAGTGGTCCGTGAATTTCAAAAAATGATCGGAGAGG
AAGCGAAGGATCAGTTGAAACGTATTGAAGGCACTATGCCTGATAAAGTAGTGGCATGTGTAGGCGGAGGAAGCAATGCG
ATGGGTATGTTTCAGGCATTTTTAAATGAAGATGTTGAACTGATCGGCGCTGAAGCAGCAGGAAAAGGAATTGATACACC
TCTTCATGCCGCCACTATTTCGAAAGGAACCGTAGGGGTTATTCACGGTTCATTGACTTATCTCATTCAGGATGAGTTCG
GGCAAATTATTGAGCCCTACTCTATTTCAGCCGGTCTCGACTATCCTGGAATCGGTCCGGAGCATGCATATTTGCATAAA
AGCGGCCGTGTCACTTATGACAGTATAACCGATGAAGAAGCGGTGGATGCATTAAAGCTTTTGTCAGAAAAAGAGGGGAT
TTTGCCGGCAATCGAATCTGCCCATGCGTTAGCGAAAGCATTCAAACTCGCCAAAGGAATGGATCGCGGTCAACTCATTC
TCGTCTGTTTATCAGGCCGGGGAGACAAGGATGTCAACACATTAATGAATGTATTGGAAGAAGAGGTGAAAGCCCATGTT
TAA

Upstream 100 bases:

>100_bases
AAATGGCAGCCAGAAGGAATTGACCTTGCCAGCGGAATTGAAAAAAACGGACAAAAAGATCAGAATCTGATGAGGCTTTT
AGAAGAAAGGATGAACCGAT

Downstream 100 bases:

>100_bases
ATTGGATCTTCAACCATCAGAAAAATTGTTTATCCCGTTTATTACGGCGGGCGATCCAGTTCCTGAGGTTTCGATTGAAC
TGGCGAAGTCACTCCAAAAA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MYPYPNEIGRYGDFGGKFVPETLMQPLDEIQTAFKQIKDDPAFREEYYKLLKDYSGRPTALTYADRVTEYLGGAKIYLKR
EDLNHTGSHKINNALGQALLAKKMGKTKIIAETGAGQHGVAAATVAAKFGFSCTVFMGEEDVARQSLNVFRMKLLGAEVV
PVTSGNGTLKDATNEAIRYWVQHCEDHFYMIGSVVGPHPYPQVVREFQKMIGEEAKDQLKRIEGTMPDKVVACVGGGSNA
MGMFQAFLNEDVELIGAEAAGKGIDTPLHAATISKGTVGVIHGSLTYLIQDEFGQIIEPYSISAGLDYPGIGPEHAYLHK
SGRVTYDSITDEEAVDALKLLSEKEGILPAIESAHALAKAFKLAKGMDRGQLILVCLSGRGDKDVNTLMNVLEEEVKAHV

Sequences:

>Translated_400_residues
MYPYPNEIGRYGDFGGKFVPETLMQPLDEIQTAFKQIKDDPAFREEYYKLLKDYSGRPTALTYADRVTEYLGGAKIYLKR
EDLNHTGSHKINNALGQALLAKKMGKTKIIAETGAGQHGVAAATVAAKFGFSCTVFMGEEDVARQSLNVFRMKLLGAEVV
PVTSGNGTLKDATNEAIRYWVQHCEDHFYMIGSVVGPHPYPQVVREFQKMIGEEAKDQLKRIEGTMPDKVVACVGGGSNA
MGMFQAFLNEDVELIGAEAAGKGIDTPLHAATISKGTVGVIHGSLTYLIQDEFGQIIEPYSISAGLDYPGIGPEHAYLHK
SGRVTYDSITDEEAVDALKLLSEKEGILPAIESAHALAKAFKLAKGMDRGQLILVCLSGRGDKDVNTLMNVLEEEVKAHV
>Mature_400_residues
MYPYPNEIGRYGDFGGKFVPETLMQPLDEIQTAFKQIKDDPAFREEYYKLLKDYSGRPTALTYADRVTEYLGGAKIYLKR
EDLNHTGSHKINNALGQALLAKKMGKTKIIAETGAGQHGVAAATVAAKFGFSCTVFMGEEDVARQSLNVFRMKLLGAEVV
PVTSGNGTLKDATNEAIRYWVQHCEDHFYMIGSVVGPHPYPQVVREFQKMIGEEAKDQLKRIEGTMPDKVVACVGGGSNA
MGMFQAFLNEDVELIGAEAAGKGIDTPLHAATISKGTVGVIHGSLTYLIQDEFGQIIEPYSISAGLDYPGIGPEHAYLHK
SGRVTYDSITDEEAVDALKLLSEKEGILPAIESAHALAKAFKLAKGMDRGQLILVCLSGRGDKDVNTLMNVLEEEVKAHV

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=385, Percent_Identity=55.8441558441558, Blast_Score=434, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6321412, Length=385, Percent_Identity=55.5844155844156, Blast_Score=447, Evalue=1e-126,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_BACSU (P07600)

Other databases:

- EMBL:   M80245
- EMBL:   K01391
- EMBL:   AL009126
- PIR:   E22794
- RefSeq:   NP_390145.2
- ProteinModelPortal:   P07600
- SMR:   P07600
- EnsemblBacteria:   EBBACT00000001131
- GeneID:   939010
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU22640
- GenoList:   BSU22640
- GeneTree:   EBGT00050000001631
- HOGENOM:   HBG303148
- OMA:   YYLREYS
- PhylomeDB:   P07600
- ProtClustDB:   PRK04346
- BioCyc:   BSUB:BSU22640-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 43580; Mature: 43580

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYPYPNEIGRYGDFGGKFVPETLMQPLDEIQTAFKQIKDDPAFREEYYKLLKDYSGRPTA
CCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCE
LTYADRVTEYLGGAKIYLKREDLNHTGSHKINNALGQALLAKKMGKTKIIAETGAGQHGV
EHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCH
AAATVAAKFGFSCTVFMGEEDVARQSLNVFRMKLLGAEVVPVTSGNGTLKDATNEAIRYW
HHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHH
VQHCEDHFYMIGSVVGPHPYPQVVREFQKMIGEEAKDQLKRIEGTMPDKVVACVGGGSNA
HHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCH
MGMFQAFLNEDVELIGAEAAGKGIDTPLHAATISKGTVGVIHGSLTYLIQDEFGQIIEPY
HHHHHHHHCCCHHEEECHHCCCCCCCCCHHHHCCCCCEEEEECHHHEEEEHHHHHHCCCC
SISAGLDYPGIGPEHAYLHKSGRVTYDSITDEEAVDALKLLSEKEGILPAIESAHALAKA
CCCCCCCCCCCCCCHHHEECCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
FKLAKGMDRGQLILVCLSGRGDKDVNTLMNVLEEEVKAHV
HHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYPYPNEIGRYGDFGGKFVPETLMQPLDEIQTAFKQIKDDPAFREEYYKLLKDYSGRPTA
CCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCE
LTYADRVTEYLGGAKIYLKREDLNHTGSHKINNALGQALLAKKMGKTKIIAETGAGQHGV
EHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCH
AAATVAAKFGFSCTVFMGEEDVARQSLNVFRMKLLGAEVVPVTSGNGTLKDATNEAIRYW
HHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHH
VQHCEDHFYMIGSVVGPHPYPQVVREFQKMIGEEAKDQLKRIEGTMPDKVVACVGGGSNA
HHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCH
MGMFQAFLNEDVELIGAEAAGKGIDTPLHAATISKGTVGVIHGSLTYLIQDEFGQIIEPY
HHHHHHHHCCCHHEEECHHCCCCCCCCCHHHHCCCCCEEEEECHHHEEEEHHHHHHCCCC
SISAGLDYPGIGPEHAYLHKSGRVTYDSITDEEAVDALKLLSEKEGILPAIESAHALAKA
CCCCCCCCCCCCCCHHHEECCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
FKLAKGMDRGQLILVCLSGRGDKDVNTLMNVLEEEVKAHV
HHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3924737; 9384377; 10568751