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The map label for this gene is sqhC

Identifier: 50812246

GI number: 50812246

Start: 2102168

End: 2104066

Strand: Direct

Name: sqhC

Synonym: BSU19320

Alternate gene names: 50812246

Gene position: 2102168-2104066 (Clockwise)

Preceding gene: 16078991

Following gene: 16078993

Centisome position: 49.87

GC content: 45.02

Gene sequence:

>1899_bases
ATGGGCACACTTCAGGAGAAAGTGAGGCGTTTTCAAAAGAAAACCATTACCGAGTTAAGAGACAGGCAAAATGCTGATGG
TTCATGGACATTTTGCTTTGAAGGACCAATCATGACAAATTCCTTTTTTATTTTGCTCCTTACCTCACTAGATGAAGGCG
AAAATGAAAAAGAACTGATATCATCCCTTGCAGCCGGCATTCATGCAAAACAGCAGCCAGACGGCACATTTATCAACTAT
CCCGATGAAACGCGCGGAAATCTAACGGCTACCGTCCAAGGATATGTCGGGATGCTGGCTTCAGGATGTTTTCACAGAAC
TGAGCCGCACATGAAGAAAGCTGAACAATTTATCATCTCACATGGCGGTTTGAGACATGTTCATTTTATGACAAAATGGA
TGCTTGCCGCGAACGGGCTTTATCCTTGGCCTGCTTTGTATTTACCATTATCACTCATGGCGCTCCCCCCAACATTGCCG
ATTCATTTCTATCAGTTCAGCTCATATGCCCGTATTCATTTTGCTCCTATGGCTGTAACACTCAATCAGCGATTTGTCCT
TATTAACCGCAATATTTCATCTCTTCACCATCTCGATCCGCACATGACAAAAAATCCTTTCACTTGGCTTCGGTCTGATG
CTTTCGAAGAAAGAGATCTCACGTCTATTTTGTTACATTGGAAACGCGTTTTTCATGCACCATTTGCTTTTCAGCAGCTG
GGCCTACAGACAGCTAAAACGTATATGCTGGACCGGATTGAAAAAGATGGAACATTATACAGCTATGCGAGCGCAACCAT
ATATATGGTTTACAGCCTTCTGTCACTTGGTGTGTCACGCTATTCTCCTATTATCAGGAGGGCGATTACCGGCATTAAAT
CACTGGTGACTAAATGCAACGGGATTCCTTATCTGGAAAACTCTACTTCAACTGTTTGGGATACAGCTTTAATAAGCTAT
GCCCTTCAAAAAAATGGTGTGACCGAAACGGATGGCTCTGTTACAAAAGCAGCCGACTTTTTGCTAGAACGCCAGCATAC
CAAAATAGCAGATTGGTCTGTCAAAAATCCAAATTCAGTTCCTGGCGGCTGGGGGTTTTCAAACATTAATACAAATAACC
CTGACTGTGACGACACTACAGCCGTTTTAAAGGCGATTCCCCGCAATCATTCTCCTGCAGCATGGGAGCGGGGGGTATCT
TGGCTTTTATCGATGCAAAACAATGACGGCGGATTTTCTGCTTTCGAAAAAAATGTGAACCATCCACTGATCCGCCTTCT
GCCGCTTGAATCCGCCGAGGACGCTGCAGTTGACCCTTCAACCGCCGACCTCACCGGACGTGTACTGCACTTTTTAGGCG
AGAAAGTTGGCTTCACAGAAAAACATCAACATATTCAACGCGCAGTGAAGTGGCTTTTCGAACATCAGGAACAAAATGGG
TCTTGGTACGGCAGATGGGGTGTTTGCTACATTTACGGCACTTGGGCTGCTCTTACTGGTATGCATGCATGCGGGGTTGA
CCGAAAGCATCCCGGTATACAAAAGGCTCTGCGTTGGCTCAAATCCATACAAAATGATGACGGAAGCTGGGGAGAATCCT
GCAAAAGCGCCGAAATCAAAACATATGTACCGCTTCATAGAGGAACCATTGTACAAACGGCCTGGGCTTTAGACGCTTTG
CTCACATATGAAAATTCCGAACATCCGTCTGTTGTGAAAGGCATGCAATACCTTACCGACAGCAGTTCGCATAGCGCCGA
TAGCCTCGCGTATCCAGCAGGGATCGGATTGCCGAAGCAATTTTATATTCGCTATCACAGTTATCCATATGTATTCTCTT
TGCTGGCTGTCGGGAAGTATTTAGATTCTATTGAAAAGGAGACAGCAAATGAAACGTGA

Upstream 100 bases:

>100_bases
CATGTATGTTTAAAAAACTGCTGCCTGCCAGCAGTTTTTTTCTTTCTGTAATGGCAGTAAAAGACGCTGAATATATATGC
TTCTGAAGGAGTTGAGCGTG

Downstream 100 bases:

>100_bases
ATCTTATCAAGCGGAGATGTTCAATTGGTGTGAAGCCCTGAAAGATCAGATTCAAAAGCGAGGTCAGCTTGACCAGTTTG
AAGATCAAATCGACAAGATG

Product: squalene-hopene cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 632; Mature: 631

Protein sequence:

>632_residues
MGTLQEKVRRFQKKTITELRDRQNADGSWTFCFEGPIMTNSFFILLLTSLDEGENEKELISSLAAGIHAKQQPDGTFINY
PDETRGNLTATVQGYVGMLASGCFHRTEPHMKKAEQFIISHGGLRHVHFMTKWMLAANGLYPWPALYLPLSLMALPPTLP
IHFYQFSSYARIHFAPMAVTLNQRFVLINRNISSLHHLDPHMTKNPFTWLRSDAFEERDLTSILLHWKRVFHAPFAFQQL
GLQTAKTYMLDRIEKDGTLYSYASATIYMVYSLLSLGVSRYSPIIRRAITGIKSLVTKCNGIPYLENSTSTVWDTALISY
ALQKNGVTETDGSVTKAADFLLERQHTKIADWSVKNPNSVPGGWGFSNINTNNPDCDDTTAVLKAIPRNHSPAAWERGVS
WLLSMQNNDGGFSAFEKNVNHPLIRLLPLESAEDAAVDPSTADLTGRVLHFLGEKVGFTEKHQHIQRAVKWLFEHQEQNG
SWYGRWGVCYIYGTWAALTGMHACGVDRKHPGIQKALRWLKSIQNDDGSWGESCKSAEIKTYVPLHRGTIVQTAWALDAL
LTYENSEHPSVVKGMQYLTDSSSHSADSLAYPAGIGLPKQFYIRYHSYPYVFSLLAVGKYLDSIEKETANET

Sequences:

>Translated_632_residues
MGTLQEKVRRFQKKTITELRDRQNADGSWTFCFEGPIMTNSFFILLLTSLDEGENEKELISSLAAGIHAKQQPDGTFINY
PDETRGNLTATVQGYVGMLASGCFHRTEPHMKKAEQFIISHGGLRHVHFMTKWMLAANGLYPWPALYLPLSLMALPPTLP
IHFYQFSSYARIHFAPMAVTLNQRFVLINRNISSLHHLDPHMTKNPFTWLRSDAFEERDLTSILLHWKRVFHAPFAFQQL
GLQTAKTYMLDRIEKDGTLYSYASATIYMVYSLLSLGVSRYSPIIRRAITGIKSLVTKCNGIPYLENSTSTVWDTALISY
ALQKNGVTETDGSVTKAADFLLERQHTKIADWSVKNPNSVPGGWGFSNINTNNPDCDDTTAVLKAIPRNHSPAAWERGVS
WLLSMQNNDGGFSAFEKNVNHPLIRLLPLESAEDAAVDPSTADLTGRVLHFLGEKVGFTEKHQHIQRAVKWLFEHQEQNG
SWYGRWGVCYIYGTWAALTGMHACGVDRKHPGIQKALRWLKSIQNDDGSWGESCKSAEIKTYVPLHRGTIVQTAWALDAL
LTYENSEHPSVVKGMQYLTDSSSHSADSLAYPAGIGLPKQFYIRYHSYPYVFSLLAVGKYLDSIEKETANET
>Mature_631_residues
GTLQEKVRRFQKKTITELRDRQNADGSWTFCFEGPIMTNSFFILLLTSLDEGENEKELISSLAAGIHAKQQPDGTFINYP
DETRGNLTATVQGYVGMLASGCFHRTEPHMKKAEQFIISHGGLRHVHFMTKWMLAANGLYPWPALYLPLSLMALPPTLPI
HFYQFSSYARIHFAPMAVTLNQRFVLINRNISSLHHLDPHMTKNPFTWLRSDAFEERDLTSILLHWKRVFHAPFAFQQLG
LQTAKTYMLDRIEKDGTLYSYASATIYMVYSLLSLGVSRYSPIIRRAITGIKSLVTKCNGIPYLENSTSTVWDTALISYA
LQKNGVTETDGSVTKAADFLLERQHTKIADWSVKNPNSVPGGWGFSNINTNNPDCDDTTAVLKAIPRNHSPAAWERGVSW
LLSMQNNDGGFSAFEKNVNHPLIRLLPLESAEDAAVDPSTADLTGRVLHFLGEKVGFTEKHQHIQRAVKWLFEHQEQNGS
WYGRWGVCYIYGTWAALTGMHACGVDRKHPGIQKALRWLKSIQNDDGSWGESCKSAEIKTYVPLHRGTIVQTAWALDALL
TYENSEHPSVVKGMQYLTDSSSHSADSLAYPAGIGLPKQFYIRYHSYPYVFSLLAVGKYLDSIEKETANET

Specific function: Catalyzes the cyclization of squalene into hopene

COG id: COG1657

COG function: function code I; Squalene cyclase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 3 PFTB repeats

Homologues:

Organism=Homo sapiens, GI224177558, Length=606, Percent_Identity=24.0924092409241, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI47933395, Length=606, Percent_Identity=24.0924092409241, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI47933397, Length=606, Percent_Identity=24.0924092409241, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI224177556, Length=590, Percent_Identity=23.8983050847458, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321863, Length=597, Percent_Identity=24.6231155778894, Blast_Score=114, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SQHC_BACSU (Q796C3)

Other databases:

- EMBL:   AF027868
- EMBL:   AL009126
- PIR:   A69718
- RefSeq:   NP_389814.2
- HSSP:   P33247
- ProteinModelPortal:   Q796C3
- EnsemblBacteria:   EBBACT00000002238
- GeneID:   939443
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU19320
- GenoList:   BSU19320
- GeneTree:   EBGT00050000002532
- HOGENOM:   HBG535807
- OMA:   YRNCFPL
- PhylomeDB:   Q796C3
- ProtClustDB:   CLSK917103
- BioCyc:   BSUB:BSU19320-MONOMER
- InterPro:   IPR001330
- InterPro:   IPR018333
- InterPro:   IPR008930
- TIGRFAMs:   TIGR01787

Pfam domain/function: PF00432 Prenyltrans; SSF48239 Terp_cyc_toroid

EC number: =5.4.99.17

Molecular weight: Translated: 71225; Mature: 71093

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS01074 TERPENE_SYNTHASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTLQEKVRRFQKKTITELRDRQNADGSWTFCFEGPIMTNSFFILLLTSLDEGENEKELI
CCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCCHHHHH
SSLAAGIHAKQQPDGTFINYPDETRGNLTATVQGYVGMLASGCFHRTEPHMKKAEQFIIS
HHHHHHCCCCCCCCCCEEECCCCCCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
HGGLRHVHFMTKWMLAANGLYPWPALYLPLSLMALPPTLPIHFYQFSSYARIHFAPMAVT
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCEEEEEEEEEEEE
LNQRFVLINRNISSLHHLDPHMTKNPFTWLRSDAFEERDLTSILLHWKRVFHAPFAFQQL
ECCEEEEEECCHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHH
GLQTAKTYMLDRIEKDGTLYSYASATIYMVYSLLSLGVSRYSPIIRRAITGIKSLVTKCN
HHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GIPYLENSTSTVWDTALISYALQKNGVTETDGSVTKAADFLLERQHTKIADWSVKNPNSV
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
PGGWGFSNINTNNPDCDDTTAVLKAIPRNHSPAAWERGVSWLLSMQNNDGGFSAFEKNVN
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHEEEEECCCCCCHHHHHHCCC
HPLIRLLPLESAEDAAVDPSTADLTGRVLHFLGEKVGFTEKHQHIQRAVKWLFEHQEQNG
CCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
SWYGRWGVCYIYGTWAALTGMHACGVDRKHPGIQKALRWLKSIQNDDGSWGESCKSAEIK
CEEECCEEEEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCCCCCE
TYVPLHRGTIVQTAWALDALLTYENSEHPSVVKGMQYLTDSSSHSADSLAYPAGIGLPKQ
EEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCE
FYIRYHSYPYVFSLLAVGKYLDSIEKETANET
EEEEECCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GTLQEKVRRFQKKTITELRDRQNADGSWTFCFEGPIMTNSFFILLLTSLDEGENEKELI
CCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCCHHHHH
SSLAAGIHAKQQPDGTFINYPDETRGNLTATVQGYVGMLASGCFHRTEPHMKKAEQFIIS
HHHHHHCCCCCCCCCCEEECCCCCCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
HGGLRHVHFMTKWMLAANGLYPWPALYLPLSLMALPPTLPIHFYQFSSYARIHFAPMAVT
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCEEEEEEEEEEEE
LNQRFVLINRNISSLHHLDPHMTKNPFTWLRSDAFEERDLTSILLHWKRVFHAPFAFQQL
ECCEEEEEECCHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHH
GLQTAKTYMLDRIEKDGTLYSYASATIYMVYSLLSLGVSRYSPIIRRAITGIKSLVTKCN
HHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GIPYLENSTSTVWDTALISYALQKNGVTETDGSVTKAADFLLERQHTKIADWSVKNPNSV
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
PGGWGFSNINTNNPDCDDTTAVLKAIPRNHSPAAWERGVSWLLSMQNNDGGFSAFEKNVN
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHEEEEECCCCCCHHHHHHCCC
HPLIRLLPLESAEDAAVDPSTADLTGRVLHFLGEKVGFTEKHQHIQRAVKWLFEHQEQNG
CCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
SWYGRWGVCYIYGTWAALTGMHACGVDRKHPGIQKALRWLKSIQNDDGSWGESCKSAEIK
CEEECCEEEEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCCCCCE
TYVPLHRGTIVQTAWALDALLTYENSEHPSVVKGMQYLTDSSSHSADSLAYPAGIGLPKQ
EEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCE
FYIRYHSYPYVFSLLAVGKYLDSIEKETANET
EEEEECCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377