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The map label for this gene is parE

Identifier: 50812242

GI number: 50812242

Start: 1933477

End: 1935444

Strand: Direct

Name: parE

Synonym: BSU18090

Alternate gene names: 50812242

Gene position: 1933477-1935444 (Clockwise)

Preceding gene: 16078869

Following gene: 255767425

Centisome position: 45.86

GC content: 45.83

Gene sequence:

>1968_bases
TTGGCTAGAAAACAGCAATTTGATTACAACGAAGATGCCATACAGGTGCTCGAAGGCCTTGAGGCTGTCAGGAAAAGGCC
GGGTATGTATATTGGCTCTACTGACGCGCGGGGTTTGCACCACTTGGTTTATGAAATTGTAGATAACTCTGTCGACGAGG
TGCTTGCCGGCCATGGAGATCACATAATCGTCAAAATACATAAAGACAATAGTATTTCTGTACAGGATAGAGGCCGCGGC
ATGCCGACCGGAATGCATAAGCTCGGCAAACCAACCCCTGAAGTGATTTTAACGGTGCTTCATGCCGGAGGGAAATTCGG
CCAAGGCGGCTATAAGACAAGCGGCGGGCTCCACGGAGTTGGTGCATCAGTCGTGAATGCTTTATCTGAATGGCTGACGG
TTACCATTGAGCGGGACGGTTTTGTCTATCAGCAGCGATTTGAAAATGGAGGAAAACCGGTTACATCTCTCGAAAAGATC
GGCAAAACAAAAAAGACGGGAACTCTTACACACTTTAAGCCAGATCCAACGATGTTCAGCACAACCACTTATAATTTTGA
AACGCTCTCTGAGCGCTTAAGAGAGTCTGCGTTTTTGTTAAAAGGACTGAAAATTGAGCTGATTGATGAACGCAATGACC
AGCGTGAGGTCTTTTATTATGAAAACGGCATAGAAGCGTTTGTTGCTTACTTAAATGAAGAAAAAGACGTCTTGTCTGAA
GTGGTCTCGTTTGAGGGTGAGCATCATTCTATAGAAGTGGACTTTGCGTTCCAGTTTAACGATGGTTATTCTGAAAATAT
TTTGTCGTTTGTCAACAATGTCAGAACAAAAGACGGCGGGACGCATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTT
TTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTT
TCAGCCATTATTTCTGTCCGCATTCCTGAGGAGCTTCTTCAATTTGAGGGACAAACAAAAGGAAAGCTCGGTACCAGTGA
AGCAAGATCAGCGGTGGATGCGATTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAATCGGGACACCGCAACGCTTC
TTGTCAAAAAAGCAATCAAGGCGAGCCAGGCGCGCGAAGCGGCACGCAAAGCCCGTGAAGAAGCAAGAAGCGGCAAAAAA
CGAAAAAAATCAGAAGCGACTTTAAGCGGAAAGCTGACGCCTGCGGGTTCAAGAAACCCGGCAAAAAATGAATTGTATCT
CGTTGAGGGAGATTCAGCAGGCGGGTCAGCCAAGCAGGGACGAGACCGCAGATTCCAGGCGGTTCTGCCTTTACGCGGTA
AAGTCATTAATACAGAAAAAGCTAAGCTTGCTGATATCTTTAAAAATGAAGAGATCAATACGATCATTCATGCGATCGGC
GGGGGAGTCGGGGCAGATTTCTCAATCGATGACATCAACTACGACAAAATCATCATTATGACCGATGCCGATACTGACGG
CGCCCATATTCAAGTGCTGCTTCTTACCTTTTTCTATCGCTATATGAAACCGCTCATTGAGCATGGCAAGGTGTTCATTG
CGCTGCCACCTTTATATAAGGTCAGTAAAGGAAGCGGGAAAAAGGAAATCATTGAATATGCATGGTCTGATGAAGAGATG
GGGGATGTCCTGAAAAAAGTCGGCAAAGGCTATACCATTCAACGATATAAGGGTCTTGGAGAGATGAACGCAGACCAGCT
GTGGGAAACGACGATGAATCCGGAGTCCCGCACACTTGTTAGGGTCAAAATTGACGATGCGGCACGTGTAGAGAGACGTG
TGACGACGTTAATGGGAGACAAAGTAGAGCCGCGCAGAAAGTGGATTGAGAAAAACGTCGCTTTCGGCTTAGATGAAGAA
AGCAATATTTTAGAAAATGAAAACTTATCGGTCGCTGAGGAGGTTTAA

Upstream 100 bases:

>100_bases
CTGGCTCGAGGCGAGGTTTATAAAAAATATCTTATATACAAATGATGTGTAACACTGAAAAGAGAAAGACAATGTTAGTT
TTTGAAAGGGGTTTGTACGT

Downstream 100 bases:

>100_bases
TGATTGTCACAGCCAGAATTATTTCATGATTTACCATTAGAAGAGGTGATCGGCGACCGTTTTGGACGCTACAGTAAATA
TATTATTCAAGACAGGGCGC

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B

Number of amino acids: Translated: 655; Mature: 654

Protein sequence:

>655_residues
MARKQQFDYNEDAIQVLEGLEAVRKRPGMYIGSTDARGLHHLVYEIVDNSVDEVLAGHGDHIIVKIHKDNSISVQDRGRG
MPTGMHKLGKPTPEVILTVLHAGGKFGQGGYKTSGGLHGVGASVVNALSEWLTVTIERDGFVYQQRFENGGKPVTSLEKI
GKTKKTGTLTHFKPDPTMFSTTTYNFETLSERLRESAFLLKGLKIELIDERNDQREVFYYENGIEAFVAYLNEEKDVLSE
VVSFEGEHHSIEVDFAFQFNDGYSENILSFVNNVRTKDGGTHESGAKTAMTRAFNEYARKVALLKEKDKNLEGTDIREGL
SAIISVRIPEELLQFEGQTKGKLGTSEARSAVDAIVSEQLAYFLEENRDTATLLVKKAIKASQAREAARKAREEARSGKK
RKKSEATLSGKLTPAGSRNPAKNELYLVEGDSAGGSAKQGRDRRFQAVLPLRGKVINTEKAKLADIFKNEEINTIIHAIG
GGVGADFSIDDINYDKIIIMTDADTDGAHIQVLLLTFFYRYMKPLIEHGKVFIALPPLYKVSKGSGKKEIIEYAWSDEEM
GDVLKKVGKGYTIQRYKGLGEMNADQLWETTMNPESRTLVRVKIDDAARVERRVTTLMGDKVEPRRKWIEKNVAFGLDEE
SNILENENLSVAEEV

Sequences:

>Translated_655_residues
MARKQQFDYNEDAIQVLEGLEAVRKRPGMYIGSTDARGLHHLVYEIVDNSVDEVLAGHGDHIIVKIHKDNSISVQDRGRG
MPTGMHKLGKPTPEVILTVLHAGGKFGQGGYKTSGGLHGVGASVVNALSEWLTVTIERDGFVYQQRFENGGKPVTSLEKI
GKTKKTGTLTHFKPDPTMFSTTTYNFETLSERLRESAFLLKGLKIELIDERNDQREVFYYENGIEAFVAYLNEEKDVLSE
VVSFEGEHHSIEVDFAFQFNDGYSENILSFVNNVRTKDGGTHESGAKTAMTRAFNEYARKVALLKEKDKNLEGTDIREGL
SAIISVRIPEELLQFEGQTKGKLGTSEARSAVDAIVSEQLAYFLEENRDTATLLVKKAIKASQAREAARKAREEARSGKK
RKKSEATLSGKLTPAGSRNPAKNELYLVEGDSAGGSAKQGRDRRFQAVLPLRGKVINTEKAKLADIFKNEEINTIIHAIG
GGVGADFSIDDINYDKIIIMTDADTDGAHIQVLLLTFFYRYMKPLIEHGKVFIALPPLYKVSKGSGKKEIIEYAWSDEEM
GDVLKKVGKGYTIQRYKGLGEMNADQLWETTMNPESRTLVRVKIDDAARVERRVTTLMGDKVEPRRKWIEKNVAFGLDEE
SNILENENLSVAEEV
>Mature_654_residues
ARKQQFDYNEDAIQVLEGLEAVRKRPGMYIGSTDARGLHHLVYEIVDNSVDEVLAGHGDHIIVKIHKDNSISVQDRGRGM
PTGMHKLGKPTPEVILTVLHAGGKFGQGGYKTSGGLHGVGASVVNALSEWLTVTIERDGFVYQQRFENGGKPVTSLEKIG
KTKKTGTLTHFKPDPTMFSTTTYNFETLSERLRESAFLLKGLKIELIDERNDQREVFYYENGIEAFVAYLNEEKDVLSEV
VSFEGEHHSIEVDFAFQFNDGYSENILSFVNNVRTKDGGTHESGAKTAMTRAFNEYARKVALLKEKDKNLEGTDIREGLS
AIISVRIPEELLQFEGQTKGKLGTSEARSAVDAIVSEQLAYFLEENRDTATLLVKKAIKASQAREAARKAREEARSGKKR
KKSEATLSGKLTPAGSRNPAKNELYLVEGDSAGGSAKQGRDRRFQAVLPLRGKVINTEKAKLADIFKNEEINTIIHAIGG
GVGADFSIDDINYDKIIIMTDADTDGAHIQVLLLTFFYRYMKPLIEHGKVFIALPPLYKVSKGSGKKEIIEYAWSDEEMG
DVLKKVGKGYTIQRYKGLGEMNADQLWETTMNPESRTLVRVKIDDAARVERRVTTLMGDKVEPRRKWIEKNVAFGLDEES
NILENENLSVAEEV

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family

Homologues:

Organism=Homo sapiens, GI19913406, Length=631, Percent_Identity=25.8320126782884, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI19913408, Length=666, Percent_Identity=24.9249249249249, Blast_Score=119, Evalue=9e-27,
Organism=Escherichia coli, GI48994957, Length=557, Percent_Identity=51.5260323159785, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI1789408, Length=637, Percent_Identity=40.0313971742543, Blast_Score=421, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17535065, Length=671, Percent_Identity=25.0372578241431, Blast_Score=138, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI212645845, Length=607, Percent_Identity=26.1943986820428, Blast_Score=112, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI212645657, Length=232, Percent_Identity=29.7413793103448, Blast_Score=83, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324241, Length=638, Percent_Identity=26.1755485893417, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17136538, Length=672, Percent_Identity=24.702380952381, Blast_Score=122, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PARE_BACSU (Q59192)

Other databases:

- EMBL:   Z73234
- EMBL:   AF024713
- EMBL:   AL009126
- PIR:   C69637
- RefSeq:   NP_389691.2
- ProteinModelPortal:   Q59192
- EnsemblBacteria:   EBBACT00000000468
- GeneID:   936151
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU18090
- NMPDR:   fig|224308.1.peg.1812
- GenoList:   BSU18090
- GeneTree:   EBGT00050000002750
- HOGENOM:   HBG304889
- OMA:   EARSGKK
- PhylomeDB:   Q59192
- ProtClustDB:   PRK05559
- BioCyc:   BSUB:BSU18090-MONOMER
- GO:   GO:0005694
- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005740
- Gene3D:   G3DSA:3.30.565.10
- Gene3D:   G3DSA:3.30.230.10
- Gene3D:   G3DSA:3.40.50.670
- PRINTS:   PR00418
- SMART:   SM00387
- SMART:   SM00433
- TIGRFAMs:   TIGR01058

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; SSF55874 ATP_bd_ATPase; SSF54211 Ribosomal_S5_D2-typ_fold; SSF56719 Topo_IIA_cen

EC number: 5.99.1.-

Molecular weight: Translated: 73008; Mature: 72876

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKQQFDYNEDAIQVLEGLEAVRKRPGMYIGSTDARGLHHLVYEIVDNSVDEVLAGHGD
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCHHHHHHCCCC
HIIVKIHKDNSISVQDRGRGMPTGMHKLGKPTPEVILTVLHAGGKFGQGGYKTSGGLHGV
EEEEEEECCCCEEEHHCCCCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCH
GASVVNALSEWLTVTIERDGFVYQQRFENGGKPVTSLEKIGKTKKTGTLTHFKPDPTMFS
HHHHHHHHHHHEEEEECCCCCHHHHHHHCCCCCHHHHHHCCCCCCCCCEEEECCCCCEEE
TTTYNFETLSERLRESAFLLKGLKIELIDERNDQREVFYYENGIEAFVAYLNEEKDVLSE
EEECCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCHHHHHHHHCCHHHHHHH
VVSFEGEHHSIEVDFAFQFNDGYSENILSFVNNVRTKDGGTHESGAKTAMTRAFNEYARK
HHHCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
VALLKEKDKNLEGTDIREGLSAIISVRIPEELLQFEGQTKGKLGTSEARSAVDAIVSEQL
HHHHHHHCCCCCCCHHHHHHHHHEEECCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
AYFLEENRDTATLLVKKAIKASQAREAARKAREEARSGKKRKKSEATLSGKLTPAGSRNP
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCC
AKNELYLVEGDSAGGSAKQGRDRRFQAVLPLRGKVINTEKAKLADIFKNEEINTIIHAIG
CCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEECCCHHHHHHHHCCCHHHHHHHHHC
GGVGADFSIDDINYDKIIIMTDADTDGAHIQVLLLTFFYRYMKPLIEHGKVFIALPPLYK
CCCCCCCEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEE
VSKGSGKKEIIEYAWSDEEMGDVLKKVGKGYTIQRYKGLGEMNADQLWETTMNPESRTLV
ECCCCCHHHHHHHHCCCHHHHHHHHHHCCCCEEHHHCCCCCCCHHHHHHHCCCCCCCEEE
RVKIDDAARVERRVTTLMGDKVEPRRKWIEKNVAFGLDEESNILENENLSVAEEV
EEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCC
>Mature Secondary Structure 
ARKQQFDYNEDAIQVLEGLEAVRKRPGMYIGSTDARGLHHLVYEIVDNSVDEVLAGHGD
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCHHHHHHCCCC
HIIVKIHKDNSISVQDRGRGMPTGMHKLGKPTPEVILTVLHAGGKFGQGGYKTSGGLHGV
EEEEEEECCCCEEEHHCCCCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCH
GASVVNALSEWLTVTIERDGFVYQQRFENGGKPVTSLEKIGKTKKTGTLTHFKPDPTMFS
HHHHHHHHHHHEEEEECCCCCHHHHHHHCCCCCHHHHHHCCCCCCCCCEEEECCCCCEEE
TTTYNFETLSERLRESAFLLKGLKIELIDERNDQREVFYYENGIEAFVAYLNEEKDVLSE
EEECCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCHHHHHHHHCCHHHHHHH
VVSFEGEHHSIEVDFAFQFNDGYSENILSFVNNVRTKDGGTHESGAKTAMTRAFNEYARK
HHHCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
VALLKEKDKNLEGTDIREGLSAIISVRIPEELLQFEGQTKGKLGTSEARSAVDAIVSEQL
HHHHHHHCCCCCCCHHHHHHHHHEEECCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
AYFLEENRDTATLLVKKAIKASQAREAARKAREEARSGKKRKKSEATLSGKLTPAGSRNP
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCC
AKNELYLVEGDSAGGSAKQGRDRRFQAVLPLRGKVINTEKAKLADIFKNEEINTIIHAIG
CCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEECCCHHHHHHHHCCCHHHHHHHHHC
GGVGADFSIDDINYDKIIIMTDADTDGAHIQVLLLTFFYRYMKPLIEHGKVFIALPPLYK
CCCCCCCEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEE
VSKGSGKKEIIEYAWSDEEMGDVLKKVGKGYTIQRYKGLGEMNADQLWETTMNPESRTLV
ECCCCCHHHHHHHHCCCHHHHHHHHHHCCCCEEHHHCCCCCCCHHHHHHHCCCCCCCEEE
RVKIDDAARVERRVTTLMGDKVEPRRKWIEKNVAFGLDEESNILENENLSVAEEV
EEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969507; 9539793; 9384377