BacMap

Definition	Bacillus subtilis subsp. subtilis str. 168 chromosome, complete genome.
Accession	NC_000964
Length	4,215,606

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The map label for this gene is gmk

Identifier: 50812226

GI number: 50812226

Start: 1641949

End: 1642563

Strand: Direct

Name: gmk

Synonym: BSU15680

Alternate gene names: 50812226

Gene position: 1641949-1642563 (Clockwise)

Preceding gene: 18677778

Following gene: 16078632

Centisome position: 38.95

GC content: 42.11

Gene sequence:

>615_bases
ATGAAAGAAAGAGGGTTATTAATCGTTCTCTCAGGTCCCTCAGGAGTTGGTAAAGGAACGGTTCGACAAGCGATCTTTTC
GCAGGAAGACACAAAATTTGAATATTCGATTTCAGTAACCACAAGAAGTCCAAGAGAGGGCGAAGTGAACGGAGTCGATT
ATTTTTTCAAAACAAGAGACGAATTCGAGCAAATGATTGCGGACAACAAGCTGCTTGAATGGGCAGAGTATGTCGGCAAT
TATTACGGCACGCCAGTCGATTATGTTGAACAGACGCTTCAAGATGGAAAAGACGTCTTTTTAGAAATTGAAGTTCAAGG
GGCTCTTCAAGTGAGAAATGCTTTCCCGGAAGGCCTGTTTATTTTCCTTGCGCCTCCAAGCCTTTCTGAACTGAAAAACA
GAATCGTGACACGAGGAACAGAAACAGACGCTCTGATTGAAAATCGAATGAAAGCCGCAAAAGCTGAGATCGAAATGATG
GATGCTTATGACTATGTCGTTGAAAACGATAATGTCGAAACGGCTTGCGATAAAATCAAAGCAATCGTTCTTGCTGAACA
TTTGAAGCGTGAACGCGTTGCACCAAGATATAAGAAAATGCTGGAGGTTGAATAA

Upstream 100 bases:

>100_bases
AGTGATCACATTATCTTATCTGCCGTCCAGCCTGAGACAGTTGCACACAGACTTTCTGTTAAAGAAGAAATTATGGATGA
AGGGCAGGGGTAATTGCCGC

Downstream 100 bases:

>100_bases
TCAATGTTAGATCCGTCAATTGATTCTTTAATGAATAAATTAGATTCAAAATATACGCTGGTGACTGTTTCTGCGAGACG
TGCCCGTGAAATGCAAATCA

Product: guanylate kinase

Products: NA

Alternate protein names: GMP kinase

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MKERGLLIVLSGPSGVGKGTVRQAIFSQEDTKFEYSISVTTRSPREGEVNGVDYFFKTRDEFEQMIADNKLLEWAEYVGN
YYGTPVDYVEQTLQDGKDVFLEIEVQGALQVRNAFPEGLFIFLAPPSLSELKNRIVTRGTETDALIENRMKAAKAEIEMM
DAYDYVVENDNVETACDKIKAIVLAEHLKRERVAPRYKKMLEVE

Sequences:

>Translated_204_residues
MKERGLLIVLSGPSGVGKGTVRQAIFSQEDTKFEYSISVTTRSPREGEVNGVDYFFKTRDEFEQMIADNKLLEWAEYVGN
YYGTPVDYVEQTLQDGKDVFLEIEVQGALQVRNAFPEGLFIFLAPPSLSELKNRIVTRGTETDALIENRMKAAKAEIEMM
DAYDYVVENDNVETACDKIKAIVLAEHLKRERVAPRYKKMLEVE
>Mature_204_residues
MKERGLLIVLSGPSGVGKGTVRQAIFSQEDTKFEYSISVTTRSPREGEVNGVDYFFKTRDEFEQMIADNKLLEWAEYVGN
YYGTPVDYVEQTLQDGKDVFLEIEVQGALQVRNAFPEGLFIFLAPPSLSELKNRIVTRGTETDALIENRMKAAKAEIEMM
DAYDYVVENDNVETACDKIKAIVLAEHLKRERVAPRYKKMLEVE

Specific function: Essential for recycling GMP and indirectly, cGMP

COG id: COG0194

COG function: function code F; Guanylate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 guanylate kinase-like domain

Homologues:

Organism=Homo sapiens, GI226874833, Length=186, Percent_Identity=35.4838709677419, Blast_Score=118, Evalue=4e-27,
Organism=Homo sapiens, GI4504221, Length=186, Percent_Identity=35.4838709677419, Blast_Score=118, Evalue=4e-27,
Organism=Homo sapiens, GI226874831, Length=186, Percent_Identity=35.4838709677419, Blast_Score=118, Evalue=4e-27,
Organism=Homo sapiens, GI14780902, Length=187, Percent_Identity=29.4117647058824, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI38570142, Length=132, Percent_Identity=37.1212121212121, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI4505237, Length=173, Percent_Identity=31.2138728323699, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI262118259, Length=173, Percent_Identity=31.2138728323699, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI262118255, Length=173, Percent_Identity=31.2138728323699, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI262118263, Length=173, Percent_Identity=31.2138728323699, Blast_Score=91, Evalue=5e-19,
Organism=Homo sapiens, GI21361598, Length=198, Percent_Identity=28.2828282828283, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI186700627, Length=122, Percent_Identity=29.5081967213115, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI186700629, Length=122, Percent_Identity=29.5081967213115, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI186972120, Length=122, Percent_Identity=29.5081967213115, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI21389483, Length=181, Percent_Identity=25.414364640884, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI192447426, Length=180, Percent_Identity=29.4444444444444, Blast_Score=71, Evalue=6e-13,
Organism=Homo sapiens, GI4557529, Length=177, Percent_Identity=29.3785310734463, Blast_Score=71, Evalue=6e-13,
Organism=Homo sapiens, GI111154074, Length=163, Percent_Identity=28.2208588957055, Blast_Score=70, Evalue=9e-13,
Organism=Homo sapiens, GI261862237, Length=179, Percent_Identity=30.1675977653631, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI119943108, Length=179, Percent_Identity=30.1675977653631, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI119943106, Length=181, Percent_Identity=30.939226519337, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI21536464, Length=187, Percent_Identity=24.5989304812834, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI148539628, Length=179, Percent_Identity=28.4916201117318, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI148539578, Length=179, Percent_Identity=28.4916201117318, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI52630447, Length=188, Percent_Identity=26.063829787234, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI218156270, Length=179, Percent_Identity=29.608938547486, Blast_Score=64, Evalue=1e-10,
Organism=Escherichia coli, GI1790080, Length=201, Percent_Identity=42.7860696517413, Blast_Score=166, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17508835, Length=172, Percent_Identity=36.046511627907, Blast_Score=120, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI193203132, Length=172, Percent_Identity=36.046511627907, Blast_Score=120, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71988048, Length=177, Percent_Identity=24.2937853107345, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI71988041, Length=177, Percent_Identity=24.2937853107345, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17557440, Length=180, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17556244, Length=134, Percent_Identity=32.8358208955224, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI71984092, Length=175, Percent_Identity=25.7142857142857, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6320662, Length=183, Percent_Identity=45.3551912568306, Blast_Score=165, Evalue=5e-42,
Organism=Drosophila melanogaster, GI21356141, Length=182, Percent_Identity=36.2637362637363, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI161077626, Length=189, Percent_Identity=30.6878306878307, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24640542, Length=189, Percent_Identity=30.6878306878307, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI45554489, Length=189, Percent_Identity=30.6878306878307, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI45554481, Length=189, Percent_Identity=30.6878306878307, Blast_Score=87, Evalue=6e-18,
Organism=Drosophila melanogaster, GI116007136, Length=189, Percent_Identity=30.6878306878307, Blast_Score=87, Evalue=6e-18,
Organism=Drosophila melanogaster, GI85724806, Length=189, Percent_Identity=30.6878306878307, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI85816106, Length=127, Percent_Identity=33.0708661417323, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI85816281, Length=127, Percent_Identity=33.0708661417323, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI45551002, Length=127, Percent_Identity=33.0708661417323, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI221330166, Length=125, Percent_Identity=33.6, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI221330164, Length=125, Percent_Identity=33.6, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI221330162, Length=125, Percent_Identity=33.6, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648808, Length=126, Percent_Identity=31.7460317460317, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281362230, Length=126, Percent_Identity=31.7460317460317, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI161078487, Length=126, Percent_Identity=31.7460317460317, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI161078485, Length=126, Percent_Identity=31.7460317460317, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648810, Length=126, Percent_Identity=31.7460317460317, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281362232, Length=126, Percent_Identity=31.7460317460317, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI161077754, Length=184, Percent_Identity=29.3478260869565, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45554791, Length=184, Percent_Identity=29.3478260869565, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45554813, Length=184, Percent_Identity=29.3478260869565, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI161077752, Length=184, Percent_Identity=29.3478260869565, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24641280, Length=184, Percent_Identity=29.3478260869565, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI281360700, Length=184, Percent_Identity=29.3478260869565, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24641282, Length=184, Percent_Identity=29.3478260869565, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45554818, Length=184, Percent_Identity=29.3478260869565, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45554804, Length=184, Percent_Identity=29.3478260869565, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KGUA_BACSU (O34328)

Other databases:

- EMBL:   Y13937
- EMBL:   AL009126
- PIR:   B69878
- RefSeq:   NP_389450.2
- ProteinModelPortal:   O34328
- SMR:   O34328
- EnsemblBacteria:   EBBACT00000001598
- GeneID:   938821
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU15680
- NMPDR:   fig|224308.1.peg.1570
- GenoList:   BSU15680
- GeneTree:   EBGT00050000002748
- HOGENOM:   HBG671982
- OMA:   EWAEFAG
- PhylomeDB:   O34328
- ProtClustDB:   PRK00300
- BioCyc:   BSUB:BSU15680-MONOMER
- BRENDA:   2.7.4.8
- GO:   GO:0005737
- HAMAP:   MF_00328
- InterPro:   IPR008144
- InterPro:   IPR008145
- InterPro:   IPR020590
- InterPro:   IPR017665
- SMART:   SM00072
- TIGRFAMs:   TIGR03263

Pfam domain/function: PF00625 Guanylate_kin

EC number: =2.7.4.8

Molecular weight: Translated: 23219; Mature: 23219

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00856 GUANYLATE_KINASE_1; PS50052 GUANYLATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKERGLLIVLSGPSGVGKGTVRQAIFSQEDTKFEYSISVTTRSPREGEVNGVDYFFKTRD
CCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCHHEECCHH
EFEQMIADNKLLEWAEYVGNYYGTPVDYVEQTLQDGKDVFLEIEVQGALQVRNAFPEGLF
HHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEEECCHHHHHHCCCCCEE
IFLAPPSLSELKNRIVTRGTETDALIENRMKAAKAEIEMMDAYDYVVENDNVETACDKIK
EEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHH
AIVLAEHLKRERVAPRYKKMLEVE
HHHHHHHHHHHCCCHHHHHHHCCC
>Mature Secondary Structure
MKERGLLIVLSGPSGVGKGTVRQAIFSQEDTKFEYSISVTTRSPREGEVNGVDYFFKTRD
CCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCHHEECCHH
EFEQMIADNKLLEWAEYVGNYYGTPVDYVEQTLQDGKDVFLEIEVQGALQVRNAFPEGLF
HHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEEECCHHHHHHCCCCCEE
IFLAPPSLSELKNRIVTRGTETDALIENRMKAAKAEIEMMDAYDYVVENDNVETACDKIK
EEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHH
AIVLAEHLKRERVAPRYKKMLEVE
HHHHHHHHHHHCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377