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The map label for this gene is nucA

Identifier: 50812179

GI number: 50812179

Start: 372154

End: 372597

Strand: Reverse

Name: nucA

Synonym: BSU03430

Alternate gene names: 50812179

Gene position: 372597-372154 (Counterclockwise)

Preceding gene: 255767103

Following gene: 16077411

Centisome position: 8.84

GC content: 47.75

Gene sequence:

>444_bases
ATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAAAGGAGA
CTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATGGCCTTCCCATCTGACCGGTATCCCG
AAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCTGAAGAA
CGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTGCAAAGA
AGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATCGGCTTA
CCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Upstream 100 bases:

>100_bases
GATTTCTGCAATAAACTGATCGTAAAACATAAAATTTAAACCTTTACACCAAATGCTTTCTGGATTACTCTTGTGTCAGT
TTTAAAAGCGGGAGGTGAAC

Downstream 100 bases:

>100_bases
ACAGTACATAGAGGAGGAACAGCACATTGATCAAATCATGGAAGCCGCAAGAACTTTCGATTTCATATCATCAATTTACT
GTATTTCAAAAGGATTCTAC

Product: endonuclease

Products: NA

Alternate protein names: Competence-specific nuclease

Number of amino acids: Translated: 147; Mature: 146

Protein sequence:

>147_residues
MTTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETMAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGAEE
RREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHRLTDYPDGTKVLFTIQ

Sequences:

>Translated_147_residues
MTTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETMAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGAEE
RREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHRLTDYPDGTKVLFTIQ
>Mature_146_residues
TTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETMAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGAEER
REQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHRLTDYPDGTKVLFTIQ

Specific function: By degrading DNA that enters the cell, plays a role in the competence of cells to be transformed. Degrades both double- stranded, linear and covalently closed circular DNA

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: To B.subtilis nucB

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUCA_BACSU (P12667)

Other databases:

- EMBL:   M21672
- EMBL:   D30762
- EMBL:   AL009126
- EMBL:   D50453
- RefSeq:   NP_388225.2
- EnsemblBacteria:   EBBACT00000000681
- GeneID:   938309
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU03430
- GenoList:   BSU03430
- GeneTree:   EBGT00050000001809
- HOGENOM:   HBG338008
- OMA:   DYPDGTK
- ProtClustDB:   CLSK887611
- BioCyc:   BSUB:BSU03430-MONOMER

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 16259; Mature: 16128

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xbe16570)-;

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETMAFPSDRYPETAKHIK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHH
DAINEGHSEVCTIDRDGAEERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPA
HHHHCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCEEEECCC
DNRGAGSWVGHRLTDYPDGTKVLFTIQ
CCCCCCCHHCCCCCCCCCCCEEEEEEH
>Mature Secondary Structure 
TTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETMAFPSDRYPETAKHIK
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHH
DAINEGHSEVCTIDRDGAEERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPA
HHHHCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCEEEECCC
DNRGAGSWVGHRLTDYPDGTKVLFTIQ
CCCCCCCHHCCCCCCCCCCCEEEEEEH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7704255; 7746143; 8969502; 9384377; 2841296