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The map label for this gene is asnH

Identifier: 255767856

GI number: 255767856

Start: 4098926

End: 4101169

Strand: Direct

Name: asnH

Synonym: BSU39920

Alternate gene names: 255767856

Gene position: 4098926-4101169 (Clockwise)

Preceding gene: 16081042

Following gene: 255767857

Centisome position: 97.23

GC content: 40.37

Gene sequence:

>2244_bases
ATGTGCGGGCTCGCGGGTATTATAAATCTGGCTGCTCCCCGTTCTCAGGAATGTACCTTTCATATATTGAAAGGCATGGC
AGATGCCATCAGCTATAGGGGACCGGATGATGAACAATATCATATTGATTCCAAAGTCGGCTTTGCATTTCGGCGATTGT
CCATTCTTGACCTTGTAAATGGACAGCAGCCCTTTTTAAATGAGGATGGATCAATCGTCGTTATGGTAAATGGAGAAATT
TATAACTATAAAGAACTGAAGGCTTCTTTACACAATCATATGTTTAAAACGACATCTGATTGTGAAGTAATTGTTCATTT
ATATGAAGAAAAAGGGATTGGATTTGTAGACGACATCATCGGAATGTTTTCAATAGCCATTTGGGACAAAAATAAAAACA
AAGTATTTTTAGTTCGTGACCGCTTTGGGATTAAACCGCTTTTTTACACAGAGCTAAAACACGAGCTTATATTTGCCTCT
GAAATAAAATCCCTATTTTCTCATCCTCACTGTCCCCGCCAATTTAATTGGAAAGAAGCACTCTCAGACATTTGGCTAAG
CGGGGAAGCAGCCAGTAATCATAAAGAAACCACTTCCTTTTTTGTAAACATCCAAAATCTCGATGCTGGCCATTATCTCG
AAATTAATTTGACAACAAATGAACGAAAAACAGCAAGCTATTGGTCTTTACAGGATATTTTGCTCAGGCAAGGCTATCGT
GAGAATCTCCATCCCGATGACCTGATTGAAGGATATAGAGAACTGCTGGCAGATTCAGTCCATAGATGTTTACAAAGCGA
CGTTGAAGTCGGGTTGTTTCTTAGCGGTGGCATTGATTCAGCTGCAGTCGCACATTTTGCTGCAGAAAAACAGGATCTCC
ATACGTTTACAGTATTAAGCCAAAGTACATTTACAAATGAAGATGCAAAATATGCCCATTGGCTGGCTAAAGATTTACAC
CTTCCAAATCATCAGGTTCTCTATCAATTAGGGAACGATGAGTTATTACAACCAGAGTCATATAAACATTTGTTATGGAT
CTGTGAAACACCGTTTTGCGGACCGGAACAATTATATAAGTTTCATTTACATAAGTATGCCAAAGCGATAAGGCCAAACC
TGAAAGTCATGCTGACCGGCCAGGGCAGCGACGAATTTAACGGCGGTTACAGCACGACACTTTCACCAGCCGAGAACCCA
AGTTGGGAAGGATTTATTGAATCGGTCAATACGATGGAGATGAACCGGCTGCATCGGCTGCAGGGAAATATCTTTAGAGT
GTGGGAGGAGCATTTTGGACTTTCTCCTATAAACCTTTCTTATTTAAAGAGCAATGATTCCAGCCAAGCAGATCCTTGGC
AATCGTATGTCCTGACAAAATACCGTGATCTGCAAATGTATAATTGCTGGCATGAAGATCGGATCGCCGCGGCTAATCAT
ATCGAAAACCGTGTACCGTTCTTGGACCATCGGCTTGTTGAATGGGTTTGCGGAATACCTGATGGACTCCGTAAAGACTT
GTTATGGGATAAGTCGGTATTGCGAAAATCATTAACAAATGAGCTGCATACAAGCTATACCCACCGGCCAAAGGTTCCTT
TTTTCTATGGTAAGGACGTGAGATATACACATAAAATGATGTTTCATCTTCTGAAGAAAAACAATTATCAGCTTATAGAG
GAGGCCTTCTCTCACAGCGATGCCTCCAGCATAATACAGGTTGAACACATTCATGCCATCATGACATATTTGGAGGATGA
TCCTGAGTTTACAAATTTTGAATTTTTATTAAGACTGGTGAACATGGGACTTTTATCAAAAATGACTAAAGAAACCCCTT
CTGTTCAATTAGACATAACCAGCCATTTAGAATCGATAACAATAAAAGACTGGCACTCACAGGAAGGTGATATTGCCAGT
CGTTTAAATATCTCAGCCAATAAGTGTGAAGGACAAGATATTTTGGCGCTAAACCCTGGCGTCACTCTGTTAAGGCCGGA
AAGCGATAGTGAGCATTGCATCTACATTGCGGAAGAAGGATTTATTCAGTTTATTGTTTCGGAGGAAGACGTCGGAGCTT
GGCTGCATATTCTCTGCGATATTAATGGAAAAGACACACTTCATACGATTTTAGACAGACATGGTGTCAGCCTTGAAGAA
GTGGCCAAATATATTCAAGAAGCCATCGAACACAATATCATTTTGATCAAACAAAAAAATCTGCCAGAAGGAGCATATAG
ATGA

Upstream 100 bases:

>100_bases
ACGCAAAACGTCTCGGAATTAAAATCGATATTGTAAAAGAAACAGATGTAGAGACAGCTTATATCTATAAACATCAATAA
CGCTATTGGGAGGGAGACAT

Downstream 100 bases:

>100_bases
GCATCTTTTATTCGTTTTCCGCACTTAGGGGATTAGCAATGGGGATTTTTTCGCCTATTTGGATTCTTTATCTGATATCT
AAAGATTATGATTTCCTTCA

Product: asparagine synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 747; Mature: 747

Protein sequence:

>747_residues
MCGLAGIINLAAPRSQECTFHILKGMADAISYRGPDDEQYHIDSKVGFAFRRLSILDLVNGQQPFLNEDGSIVVMVNGEI
YNYKELKASLHNHMFKTTSDCEVIVHLYEEKGIGFVDDIIGMFSIAIWDKNKNKVFLVRDRFGIKPLFYTELKHELIFAS
EIKSLFSHPHCPRQFNWKEALSDIWLSGEAASNHKETTSFFVNIQNLDAGHYLEINLTTNERKTASYWSLQDILLRQGYR
ENLHPDDLIEGYRELLADSVHRCLQSDVEVGLFLSGGIDSAAVAHFAAEKQDLHTFTVLSQSTFTNEDAKYAHWLAKDLH
LPNHQVLYQLGNDELLQPESYKHLLWICETPFCGPEQLYKFHLHKYAKAIRPNLKVMLTGQGSDEFNGGYSTTLSPAENP
SWEGFIESVNTMEMNRLHRLQGNIFRVWEEHFGLSPINLSYLKSNDSSQADPWQSYVLTKYRDLQMYNCWHEDRIAAANH
IENRVPFLDHRLVEWVCGIPDGLRKDLLWDKSVLRKSLTNELHTSYTHRPKVPFFYGKDVRYTHKMMFHLLKKNNYQLIE
EAFSHSDASSIIQVEHIHAIMTYLEDDPEFTNFEFLLRLVNMGLLSKMTKETPSVQLDITSHLESITIKDWHSQEGDIAS
RLNISANKCEGQDILALNPGVTLLRPESDSEHCIYIAEEGFIQFIVSEEDVGAWLHILCDINGKDTLHTILDRHGVSLEE
VAKYIQEAIEHNIILIKQKNLPEGAYR

Sequences:

>Translated_747_residues
MCGLAGIINLAAPRSQECTFHILKGMADAISYRGPDDEQYHIDSKVGFAFRRLSILDLVNGQQPFLNEDGSIVVMVNGEI
YNYKELKASLHNHMFKTTSDCEVIVHLYEEKGIGFVDDIIGMFSIAIWDKNKNKVFLVRDRFGIKPLFYTELKHELIFAS
EIKSLFSHPHCPRQFNWKEALSDIWLSGEAASNHKETTSFFVNIQNLDAGHYLEINLTTNERKTASYWSLQDILLRQGYR
ENLHPDDLIEGYRELLADSVHRCLQSDVEVGLFLSGGIDSAAVAHFAAEKQDLHTFTVLSQSTFTNEDAKYAHWLAKDLH
LPNHQVLYQLGNDELLQPESYKHLLWICETPFCGPEQLYKFHLHKYAKAIRPNLKVMLTGQGSDEFNGGYSTTLSPAENP
SWEGFIESVNTMEMNRLHRLQGNIFRVWEEHFGLSPINLSYLKSNDSSQADPWQSYVLTKYRDLQMYNCWHEDRIAAANH
IENRVPFLDHRLVEWVCGIPDGLRKDLLWDKSVLRKSLTNELHTSYTHRPKVPFFYGKDVRYTHKMMFHLLKKNNYQLIE
EAFSHSDASSIIQVEHIHAIMTYLEDDPEFTNFEFLLRLVNMGLLSKMTKETPSVQLDITSHLESITIKDWHSQEGDIAS
RLNISANKCEGQDILALNPGVTLLRPESDSEHCIYIAEEGFIQFIVSEEDVGAWLHILCDINGKDTLHTILDRHGVSLEE
VAKYIQEAIEHNIILIKQKNLPEGAYR
>Mature_747_residues
MCGLAGIINLAAPRSQECTFHILKGMADAISYRGPDDEQYHIDSKVGFAFRRLSILDLVNGQQPFLNEDGSIVVMVNGEI
YNYKELKASLHNHMFKTTSDCEVIVHLYEEKGIGFVDDIIGMFSIAIWDKNKNKVFLVRDRFGIKPLFYTELKHELIFAS
EIKSLFSHPHCPRQFNWKEALSDIWLSGEAASNHKETTSFFVNIQNLDAGHYLEINLTTNERKTASYWSLQDILLRQGYR
ENLHPDDLIEGYRELLADSVHRCLQSDVEVGLFLSGGIDSAAVAHFAAEKQDLHTFTVLSQSTFTNEDAKYAHWLAKDLH
LPNHQVLYQLGNDELLQPESYKHLLWICETPFCGPEQLYKFHLHKYAKAIRPNLKVMLTGQGSDEFNGGYSTTLSPAENP
SWEGFIESVNTMEMNRLHRLQGNIFRVWEEHFGLSPINLSYLKSNDSSQADPWQSYVLTKYRDLQMYNCWHEDRIAAANH
IENRVPFLDHRLVEWVCGIPDGLRKDLLWDKSVLRKSLTNELHTSYTHRPKVPFFYGKDVRYTHKMMFHLLKKNNYQLIE
EAFSHSDASSIIQVEHIHAIMTYLEDDPEFTNFEFLLRLVNMGLLSKMTKETPSVQLDITSHLESITIKDWHSQEGDIAS
RLNISANKCEGQDILALNPGVTLLRPESDSEHCIYIAEEGFIQFIVSEEDVGAWLHILCDINGKDTLHTILDRHGVSLEE
VAKYIQEAIEHNIILIKQKNLPEGAYR

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain

Homologues:

Organism=Homo sapiens, GI168229248, Length=415, Percent_Identity=26.5060240963855, Blast_Score=100, Evalue=8e-21,
Organism=Homo sapiens, GI168229252, Length=415, Percent_Identity=26.5060240963855, Blast_Score=100, Evalue=8e-21,
Organism=Homo sapiens, GI168229250, Length=415, Percent_Identity=26.5060240963855, Blast_Score=100, Evalue=8e-21,
Organism=Homo sapiens, GI296010848, Length=386, Percent_Identity=26.6839378238342, Blast_Score=97, Evalue=6e-20,
Organism=Escherichia coli, GI1786889, Length=569, Percent_Identity=26.713532513181, Blast_Score=143, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6321563, Length=571, Percent_Identity=23.292469352014, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6325403, Length=458, Percent_Identity=25.3275109170306, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI45553209, Length=486, Percent_Identity=26.3374485596708, Blast_Score=129, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASNH_BACSU (P42113)

Other databases:

- EMBL:   AB005554
- EMBL:   AL009126
- PIR:   A69591
- RefSeq:   NP_391871.2
- ProteinModelPortal:   P42113
- SMR:   P42113
- EnsemblBacteria:   EBBACT00000003126
- GeneID:   937677
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU39920
- NMPDR:   fig|224308.1.peg.3997
- GenoList:   BSU39920
- GeneTree:   EBGT00070000032622
- BioCyc:   BSUB:BSU39920-MONOMER
- BRENDA:   6.3.5.4
- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR01536

Pfam domain/function: PF00733 Asn_synthase

EC number: =6.3.5.4

Molecular weight: Translated: 85933; Mature: 85933

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS51278 GATASE_TYPE_2

Important sites: ACT_SITE 2-2

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGLAGIINLAAPRSQECTFHILKGMADAISYRGPDDEQYHIDSKVGFAFRRLSILDLVN
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHC
GQQPFLNEDGSIVVMVNGEIYNYKELKASLHNHMFKTTSDCEVIVHLYEEKGIGFVDDII
CCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEHHCCCCCHHHHHH
GMFSIAIWDKNKNKVFLVRDRFGIKPLFYTELKHELIFASEIKSLFSHPHCPRQFNWKEA
HHHEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
LSDIWLSGEAASNHKETTSFFVNIQNLDAGHYLEINLTTNERKTASYWSLQDILLRQGYR
HHHHEECCCCCCCCCCCEEEEEEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCC
ENLHPDDLIEGYRELLADSVHRCLQSDVEVGLFLSGGIDSAAVAHFAAEKQDLHTFTVLS
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEEEE
QSTFTNEDAKYAHWLAKDLHLPNHQVLYQLGNDELLQPESYKHLLWICETPFCGPEQLYK
CCCCCCCCHHHHHHHHHHCCCCCHHEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHH
FHLHKYAKAIRPNLKVMLTGQGSDEFNGGYSTTLSPAENPSWEGFIESVNTMEMNRLHRL
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
QGNIFRVWEEHFGLSPINLSYLKSNDSSQADPWQSYVLTKYRDLQMYNCWHEDRIAAANH
HCHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCHHCCCCCHHHHHHHHH
IENRVPFLDHRLVEWVCGIPDGLRKDLLWDKSVLRKSLTNELHTSYTHRPKVPFFYGKDV
HHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCC
RYTHKMMFHLLKKNNYQLIEEAFSHSDASSIIQVEHIHAIMTYLEDDPEFTNFEFLLRLV
HHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
NMGLLSKMTKETPSVQLDITSHLESITIKDWHSQEGDIASRLNISANKCEGQDILALNPG
HHHHHHHHHHCCCCEEEEEHHCCCEEEEECCCCCCCCHHHHCCCCCCCCCCCEEEEECCC
VTLLRPESDSEHCIYIAEEGFIQFIVSEEDVGAWLHILCDINGKDTLHTILDRHGVSLEE
EEEECCCCCCCCEEEEECCCEEEEEECCCCCCEEEEEEEECCCCHHHHHHHHHCCCCHHH
VAKYIQEAIEHNIILIKQKNLPEGAYR
HHHHHHHHHHCCEEEEEECCCCCCCCC
>Mature Secondary Structure
MCGLAGIINLAAPRSQECTFHILKGMADAISYRGPDDEQYHIDSKVGFAFRRLSILDLVN
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHC
GQQPFLNEDGSIVVMVNGEIYNYKELKASLHNHMFKTTSDCEVIVHLYEEKGIGFVDDII
CCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEHHCCCCCHHHHHH
GMFSIAIWDKNKNKVFLVRDRFGIKPLFYTELKHELIFASEIKSLFSHPHCPRQFNWKEA
HHHEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
LSDIWLSGEAASNHKETTSFFVNIQNLDAGHYLEINLTTNERKTASYWSLQDILLRQGYR
HHHHEECCCCCCCCCCCEEEEEEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCC
ENLHPDDLIEGYRELLADSVHRCLQSDVEVGLFLSGGIDSAAVAHFAAEKQDLHTFTVLS
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEEEE
QSTFTNEDAKYAHWLAKDLHLPNHQVLYQLGNDELLQPESYKHLLWICETPFCGPEQLYK
CCCCCCCCHHHHHHHHHHCCCCCHHEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHH
FHLHKYAKAIRPNLKVMLTGQGSDEFNGGYSTTLSPAENPSWEGFIESVNTMEMNRLHRL
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
QGNIFRVWEEHFGLSPINLSYLKSNDSSQADPWQSYVLTKYRDLQMYNCWHEDRIAAANH
HCHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCHHCCCCCHHHHHHHHH
IENRVPFLDHRLVEWVCGIPDGLRKDLLWDKSVLRKSLTNELHTSYTHRPKVPFFYGKDV
HHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCC
RYTHKMMFHLLKKNNYQLIEEAFSHSDASSIIQVEHIHAIMTYLEDDPEFTNFEFLLRLV
HHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
NMGLLSKMTKETPSVQLDITSHLESITIKDWHSQEGDIASRLNISANKCEGQDILALNPG
HHHHHHHHHHCCCCEEEEEHHCCCEEEEECCCCCCCCHHHHCCCCCCCCCCCEEEEECCC
VTLLRPESDSEHCIYIAEEGFIQFIVSEEDVGAWLHILCDINGKDTLHTILDRHGVSLEE
EEEECCCCCCCCEEEEECCCEEEEEECCCCCCEEEEEEEECCCCHHHHHHHHHCCCCHHH
VAKYIQEAIEHNIILIKQKNLPEGAYR
HHHHHHHHHHCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584049; 9384377; 10498721