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The map label for this gene is iolB

Identifier: 255767853

GI number: 255767853

Start: 4082030

End: 4082845

Strand: Reverse

Name: iolB

Synonym: BSU39750

Alternate gene names: 255767853

Gene position: 4082845-4082030 (Counterclockwise)

Preceding gene: 16081027

Following gene: 16081025

Centisome position: 96.85

GC content: 45.47

Gene sequence:

>816_bases
ATGAGTTATTTGTTGCGTAAGCCGCAGTCGCATGAAGTGTCTAATGGGGTCAAACTCGTGCACGAAGTAACGACATCCAA
CTCTGATCTCACTTATGTAGAGTTTAAAGTGTTAGATCTTGCATCAGGTTCAAGCTATACAGAAGAATTGAAAAAACAAG
AAATCTGTATTGTGGCGGTAACGGGGAAAATTACAGTGACAGATCATGAGTCGACTTTTGAGAATATCGGCACGCGCGAA
AGCGTATTTGAACGAAAACCGACAGACAGCGTCTATATTTCAAATGACCGTGCATTTGAGATCACAGCGGTCAGCGACGC
AAGAGTGGCGCTTTGCTATTCTCCATCGGAAAAGCAGCTTCCGACAAAGCTGATCAAAGCGGAAGACAACGGAATTGAGC
ATCGCGGGCAATTTTCAAACAAACGTACTGTTCATAACATTCTTCCGGATTCAGACCCTTCAGCTAACAGTCTATTAGTA
GTTGAAGTCTATACAGACAGCGGCAACTGGTCCAGCTACCCGCCTCACAAACATGACCAAGACAACTTGCCGGAAGAATC
TTTCTTAGAAGAAACGTACTACCATGAGTTAGACCCGGGACAGGGCTTTGTGTTTCAGCGCGTATACACAGATGACCGTT
CTATTGACGAGACAATGACTGTGGGAAATGAAAACGTTGTCATCGTTCCTGCGGGATACCACCCGGTAGGCGTTCCGGAC
GGATACACATCCTACTATTTAAATGTCATGGCAGGGCCGACGCGAAAATGGAAGTTTTATAACGACCCGGCGCATGAATG
GATTTTAGAACGCTAA

Upstream 100 bases:

>100_bases
GATATCCGGCACCTGATTTCAACTAAACGACGAACAGCCGAACGGCGTCCATGTGCGCTGTTCGGACTCATTCTGCCAAT
AGAAAGAGGAGGGCTTCTCT

Downstream 100 bases:

>100_bases
CAAGTGAGGAGTGGCTGTTTACGATGAAGTATACATTCAATGAAGAGAAGGCTTTTGATATTGTTGCCATCGGCCGGGCA
TGTATTGATCTGAACGCAGT

Product: 5-deoxy-D-glucuronic acid isomerase

Products: NA

Alternate protein names: 5DG isomerase [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSYLLRKPQSHEVSNGVKLVHEVTTSNSDLTYVEFKVLDLASGSSYTEELKKQEICIVAVTGKITVTDHESTFENIGTRE
SVFERKPTDSVYISNDRAFEITAVSDARVALCYSPSEKQLPTKLIKAEDNGIEHRGQFSNKRTVHNILPDSDPSANSLLV
VEVYTDSGNWSSYPPHKHDQDNLPEESFLEETYYHELDPGQGFVFQRVYTDDRSIDETMTVGNENVVIVPAGYHPVGVPD
GYTSYYLNVMAGPTRKWKFYNDPAHEWILER

Sequences:

>Translated_271_residues
MSYLLRKPQSHEVSNGVKLVHEVTTSNSDLTYVEFKVLDLASGSSYTEELKKQEICIVAVTGKITVTDHESTFENIGTRE
SVFERKPTDSVYISNDRAFEITAVSDARVALCYSPSEKQLPTKLIKAEDNGIEHRGQFSNKRTVHNILPDSDPSANSLLV
VEVYTDSGNWSSYPPHKHDQDNLPEESFLEETYYHELDPGQGFVFQRVYTDDRSIDETMTVGNENVVIVPAGYHPVGVPD
GYTSYYLNVMAGPTRKWKFYNDPAHEWILER
>Mature_270_residues
SYLLRKPQSHEVSNGVKLVHEVTTSNSDLTYVEFKVLDLASGSSYTEELKKQEICIVAVTGKITVTDHESTFENIGTRES
VFERKPTDSVYISNDRAFEITAVSDARVALCYSPSEKQLPTKLIKAEDNGIEHRGQFSNKRTVHNILPDSDPSANSLLVV
EVYTDSGNWSSYPPHKHDQDNLPEESFLEETYYHELDPGQGFVFQRVYTDDRSIDETMTVGNENVVIVPAGYHPVGVPDG
YTSYYLNVMAGPTRKWKFYNDPAHEWILER

Specific function: Involved in the isomerization of 5-deoxy-glucuronate (5DG) to 5-dehydro-2-deoxy-D-gluconate (DKG or 2-deoxy-5-keto-D- gluconate) [H]

COG id: COG3718

COG function: function code G; Uncharacterized enzyme involved in inositol metabolism

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isomerase iolB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR021120
- InterPro:   IPR014710 [H]

Pfam domain/function: PF04962 KduI [H]

EC number: NA

Molecular weight: Translated: 30706; Mature: 30575

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYLLRKPQSHEVSNGVKLVHEVTTSNSDLTYVEFKVLDLASGSSYTEELKKQEICIVAV
CCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEEEEEEECCCCHHHHHHHHCCEEEEEE
TGKITVTDHESTFENIGTRESVFERKPTDSVYISNDRAFEITAVSDARVALCYSPSEKQL
ECEEEEECCHHHHHHCCCHHHHHHCCCCCCEEECCCCEEEEEEECCCEEEEEECCCCCCC
PTKLIKAEDNGIEHRGQFSNKRTVHNILPDSDPSANSLLVVEVYTDSGNWSSYPPHKHDQ
CHHHEECCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCC
DNLPEESFLEETYYHELDPGQGFVFQRVYTDDRSIDETMTVGNENVVIVPAGYHPVGVPD
CCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHEEECCCCEEEEECCCCCCCCCC
GYTSYYLNVMAGPTRKWKFYNDPAHEWILER
CCCEEEEEEEECCCCCEEEECCCHHHHCCCC
>Mature Secondary Structure 
SYLLRKPQSHEVSNGVKLVHEVTTSNSDLTYVEFKVLDLASGSSYTEELKKQEICIVAV
CCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEEEEEEECCCCHHHHHHHHCCEEEEEE
TGKITVTDHESTFENIGTRESVFERKPTDSVYISNDRAFEITAVSDARVALCYSPSEKQL
ECEEEEECCHHHHHHCCCHHHHHHCCCCCCEEECCCCEEEEEEECCCEEEEEECCCCCCC
PTKLIKAEDNGIEHRGQFSNKRTVHNILPDSDPSANSLLVVEVYTDSGNWSSYPPHKHDQ
CHHHEECCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCC
DNLPEESFLEETYYHELDPGQGFVFQRVYTDDRSIDETMTVGNENVVIVPAGYHPVGVPD
CCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHEEECCCCEEEEECCCCCCCCCC
GYTSYYLNVMAGPTRKWKFYNDPAHEWILER
CCCEEEEEEEECCCCCEEEECCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA