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The map label for this gene is yxeI

Identifier: 255767844

GI number: 255767844

Start: 4062543

End: 4063529

Strand: Reverse

Name: yxeI

Synonym: BSU39540

Alternate gene names: 255767844

Gene position: 4063529-4062543 (Counterclockwise)

Preceding gene: 16081006

Following gene: 255767843

Centisome position: 96.39

GC content: 43.97

Gene sequence:

>987_bases
ATGTGCACAAGTCTTACATTGGAAACTGCTGACCGTAAACATGTATTAGCAAGAACAATGGATTTTGCATTTCAGCTGGG
GACAGAGGTGATTCTCTATCCGCGCCGTTACAGCTGGAACAGTGAAGCTGACGGAAGGGCCCATCAGACACAGTACGCGT
TTATCGGTATGGGGAGAAAACTTGGAAATATATTATTTGCCGACGGCATTAATGAAAGCGGTTTATCTTGTGCGGCGCTT
TATTTTCCGGGCTATGCGGAGTACGAAAAAACGATACGGGAAGATACCGTTCACATTGTCCCGCATGAGTTTGTGACATG
GGTGCTGTCAGTCTGTCAGTCTTTGGAAGACGTAAAAGAAAAGATTCGATCTTTAACGATTGTAGAGAAAAAATTAGATC
TATTGGATACAGTTTTACCGCTTCACTGGATATTGTCAGACCGGACGGGCCGAAACCTGACGATAGAACCAAGAGCAGAC
GGCCTCAAAGTCTATGATAATCAACCTGGTGTCATGACGAACAGCCCCGACTTTATATGGCATGTAACCAATCTGCAGCA
ATATACAGGAATCAGACCGAAGCAATTAGAGAGCAAAGAGATGGGCGGATTAGCCCTTTCTGCTTTTGGCCAAGGCTTAG
GAACTGTTGGTCTGCCGGGGGATTATACACCGCCTTCCCGGTTTGTCAGAGCTGTTTATTTGAAAGAACATCTAGAGCCG
GCGGCCGATGAAACGAAAGGTGTAACAGCCGCTTTTCAAATTTTGGCAAATATGACGATACCAAAGGGCGCAGTGATAAC
GGAAGAAGACGAAATTCATTATACGCAATATACTTCCGTGATGTGCAACGAAACTGGAAACTACTATTTCCACCACTATG
ACAATCGGCAAATCCAAAAAGTAAATTTATTTCATGAAGACCTTGACTGTTTGGAGCCTAAAGTGTTTTCCGCTAAAGCA
GAAGAGAGTATTCATGAGCTTAATTAA

Upstream 100 bases:

>100_bases
CAATTCTTTGACGAATCGATTTGACTTTCGTTATGCTGATAAAACCAATTCGTTCACAATATGTATGATATTAATCTGAA
TTGAAAAGGGGACTGATCAA

Downstream 100 bases:

>100_bases
TTCACATCAATCAGCTGATTCCGGTTTGAATATCTGGAGTCAGCTGTTTTTTTATACAATCGTTACGGCGTCTGAATGGA
TAGGCGGTTTGCAAAAGCGA

Product: hydrolase

Products: carboxylate; 6-aminopenicillanate

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MCTSLTLETADRKHVLARTMDFAFQLGTEVILYPRRYSWNSEADGRAHQTQYAFIGMGRKLGNILFADGINESGLSCAAL
YFPGYAEYEKTIREDTVHIVPHEFVTWVLSVCQSLEDVKEKIRSLTIVEKKLDLLDTVLPLHWILSDRTGRNLTIEPRAD
GLKVYDNQPGVMTNSPDFIWHVTNLQQYTGIRPKQLESKEMGGLALSAFGQGLGTVGLPGDYTPPSRFVRAVYLKEHLEP
AADETKGVTAAFQILANMTIPKGAVITEEDEIHYTQYTSVMCNETGNYYFHHYDNRQIQKVNLFHEDLDCLEPKVFSAKA
EESIHELN

Sequences:

>Translated_328_residues
MCTSLTLETADRKHVLARTMDFAFQLGTEVILYPRRYSWNSEADGRAHQTQYAFIGMGRKLGNILFADGINESGLSCAAL
YFPGYAEYEKTIREDTVHIVPHEFVTWVLSVCQSLEDVKEKIRSLTIVEKKLDLLDTVLPLHWILSDRTGRNLTIEPRAD
GLKVYDNQPGVMTNSPDFIWHVTNLQQYTGIRPKQLESKEMGGLALSAFGQGLGTVGLPGDYTPPSRFVRAVYLKEHLEP
AADETKGVTAAFQILANMTIPKGAVITEEDEIHYTQYTSVMCNETGNYYFHHYDNRQIQKVNLFHEDLDCLEPKVFSAKA
EESIHELN
>Mature_328_residues
MCTSLTLETADRKHVLARTMDFAFQLGTEVILYPRRYSWNSEADGRAHQTQYAFIGMGRKLGNILFADGINESGLSCAAL
YFPGYAEYEKTIREDTVHIVPHEFVTWVLSVCQSLEDVKEKIRSLTIVEKKLDLLDTVLPLHWILSDRTGRNLTIEPRAD
GLKVYDNQPGVMTNSPDFIWHVTNLQQYTGIRPKQLESKEMGGLALSAFGQGLGTVGLPGDYTPPSRFVRAVYLKEHLEP
AADETKGVTAAFQILANMTIPKGAVITEEDEIHYTQYTSVMCNETGNYYFHHYDNRQIQKVNLFHEDLDCLEPKVFSAKA
EESIHELN

Specific function: Unknown

COG id: COG3049

COG function: function code M; Penicillin V acylase and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C59 family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YXEI_BACSU (P54948)

Other databases:

- EMBL:   D45912
- EMBL:   AL009126
- PIR:   C70075
- RefSeq:   NP_391833.2
- PDB:   2OQC
- PDBsum:   2OQC
- ProteinModelPortal:   P54948
- SMR:   P54948
- EnsemblBacteria:   EBBACT00000002124
- GeneID:   937573
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU39540
- NMPDR:   fig|224308.1.peg.3959
- GenoList:   BSU39540
- GeneTree:   EBGT00050000002550
- HOGENOM:   HBG694026
- ProtClustDB:   CLSK888160
- BioCyc:   BSUB:BSU39540-MONOMER
- InterPro:   IPR003199

Pfam domain/function: PF02275 CBAH

EC number: 3.5.1.11

Molecular weight: Translated: 37127; Mature: 37127

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCTSLTLETADRKHVLARTMDFAFQLGTEVILYPRRYSWNSEADGRAHQTQYAFIGMGRK
CCCCEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHCEEEEEECCHH
LGNILFADGINESGLSCAALYFPGYAEYEKTIREDTVHIVPHEFVTWVLSVCQSLEDVKE
HCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHH
KIRSLTIVEKKLDLLDTVLPLHWILSDRTGRNLTIEPRADGLKVYDNQPGVMTNSPDFIW
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCCCCEECCCCEEE
HVTNLQQYTGIRPKQLESKEMGGLALSAFGQGLGTVGLPGDYTPPSRFVRAVYLKEHLEP
EECCHHHHCCCCCHHCCHHHCCCEEHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
AADETKGVTAAFQILANMTIPKGAVITEEDEIHYTQYTSVMCNETGNYYFHHYDNRQIQK
CCHHHCCHHHHHHHHHHCCCCCCCEEECCCCEEEEEHHEEEECCCCCEEEEECCCCEEEE
VNLFHEDLDCLEPKVFSAKAEESIHELN
EHHHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCTSLTLETADRKHVLARTMDFAFQLGTEVILYPRRYSWNSEADGRAHQTQYAFIGMGRK
CCCCEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHCEEEEEECCHH
LGNILFADGINESGLSCAALYFPGYAEYEKTIREDTVHIVPHEFVTWVLSVCQSLEDVKE
HCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHH
KIRSLTIVEKKLDLLDTVLPLHWILSDRTGRNLTIEPRADGLKVYDNQPGVMTNSPDFIW
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCCCCEECCCCEEE
HVTNLQQYTGIRPKQLESKEMGGLALSAFGQGLGTVGLPGDYTPPSRFVRAVYLKEHLEP
EECCHHHHCCCCCHHCCHHHCCCEEHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
AADETKGVTAAFQILANMTIPKGAVITEEDEIHYTQYTSVMCNETGNYYFHHYDNRQIQK
CCHHHCCHHHHHHHHHHCCCCCCCEEECCCCEEEEEHHEEEECCCCCEEEEECCCCEEEE
VNLFHEDLDCLEPKVFSAKAEESIHELN
EHHHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: penicillin; H2O

Specific reaction: penicillin + H2O = a carboxylate + 6-aminopenicillanate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8867804; 9384377