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The map label for this gene is rocG

Identifier: 255767794

GI number: 255767794

Start: 3880740

End: 3882014

Strand: Reverse

Name: rocG

Synonym: BSU37790

Alternate gene names: 255767794

Gene position: 3882014-3880740 (Counterclockwise)

Preceding gene: 16080832

Following gene: 16080830

Centisome position: 92.09

GC content: 49.57

Gene sequence:

>1275_bases
ATGTCAGCAAAGCAAGTCTCGAAAGATGAAGAAAAAGAAGCTCTTAACTTATTTCTGTCTACCCAAACAATCATTAAGGA
AGCCCTTCGGAAGCTGGGTTATCCGGGAGATATGTATGAACTCATGAAAGAGCCGCAGAGAATGCTCACTGTCCGCATTC
CGGTCAAAATGGACAATGGGAGCGTCAAAGTGTTCACAGGCTACCGGTCACAGCACAATGATGCTGTCGGTCCGACAAAG
GGGGGCGTTCGCTTCCATCCAGAAGTTAATGAAGAGGAAGTAAAGGCATTATCCATTTGGATGACGCTCAAATGCGGGAT
TGCCAATCTTCCTTACGGCGGCGGGAAGGGCGGTATTATTTGTGATCCGCGGACAATGTCATTTGGAGAACTGGAAAGGC
TGAGCAGGGGGTATGTCCGTGCCATCAGCCAGATCGTCGGTCCGACAAAGGATATTCCAGCTCCCGATGTGTACACCAAT
TCGCAGATTATGGCGTGGATGATGGATGAGTACAGCCGGCTGCGGGAATTCGATTCTCCGGGCTTTATTACAGGTAAACC
GCTTGTTTTGGGAGGATCGCAAGGACGGGAAACAGCGACGGCACAGGGCGTCACGATTTGTATTGAAGAGGCGGTGAAGA
AAAAAGGGATCAAGCTGCAAAACGCGCGCATCATCATACAGGGCTTTGGAAACGCGGGTAGCTTCCTGGCCAAATTCATG
CACGATGCGGGCGCGAAGGTGATCGGGATTTCTGATGCCAATGGCGGGCTCTACAACCCAGACGGCCTTGATATCCCTTA
TTTGCTCGATAAACGGGACAGCTTTGGTATGGTCACCAATTTATTTACTGACGTCATCACAAATGAGGAGCTGCTTGAAA
AGGATTGCGATATTTTAGTGCCTGCCGCGATCTCCAATCAAATCACAGCCAAAAACGCACATAACATTCAGGCGTCAATC
GTCGTTGAAGCGGCGAACGGCCCGACAACCATTGATGCCACTAAGATCCTGAATGAAAGAGGCGTGCTGCTTGTGCCGGA
TATCCTAGCGAGTGCCGGCGGCGTCACGGTTTCTTATTTTGAATGGGTGCAAAACAACCAAGGATATTATTGGTCGGAAG
AAGAGGTTGCAGAAAAACTGAGAAGCGTCATGGTCAGCTCGTTCGAAACAATTTATCAAACAGCGGCAACACATAAAGTG
GATATGCGTTTGGCGGCTTACATGACGGGCATCAGAAAATCGGCAGAAGCATCGCGTTTCCGCGGATGGGTCTAA

Upstream 100 bases:

>100_bases
CATGATGATAAGGGTGAATCCAATGAAGAAAGGAATTGGCGGGCTGATTTTTTAAAGCGCTTACATTACAGCCGGCCAAA
AAAACAATGAGGTGAAAAAG

Downstream 100 bases:

>100_bases
TTTGAGAAGCCTCCGCAAAATAATTTTGCATTTGTTTTTTGCGTAAAGAGCAAAAAACGCAAAATAAATTTGCGTTCAAG
ATCGGAAAAAGAGAAAGGTG

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NAD-GDH

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MSAKQVSKDEEKEALNLFLSTQTIIKEALRKLGYPGDMYELMKEPQRMLTVRIPVKMDNGSVKVFTGYRSQHNDAVGPTK
GGVRFHPEVNEEEVKALSIWMTLKCGIANLPYGGGKGGIICDPRTMSFGELERLSRGYVRAISQIVGPTKDIPAPDVYTN
SQIMAWMMDEYSRLREFDSPGFITGKPLVLGGSQGRETATAQGVTICIEEAVKKKGIKLQNARIIIQGFGNAGSFLAKFM
HDAGAKVIGISDANGGLYNPDGLDIPYLLDKRDSFGMVTNLFTDVITNEELLEKDCDILVPAAISNQITAKNAHNIQASI
VVEAANGPTTIDATKILNERGVLLVPDILASAGGVTVSYFEWVQNNQGYYWSEEEVAEKLRSVMVSSFETIYQTAATHKV
DMRLAAYMTGIRKSAEASRFRGWV

Sequences:

>Translated_424_residues
MSAKQVSKDEEKEALNLFLSTQTIIKEALRKLGYPGDMYELMKEPQRMLTVRIPVKMDNGSVKVFTGYRSQHNDAVGPTK
GGVRFHPEVNEEEVKALSIWMTLKCGIANLPYGGGKGGIICDPRTMSFGELERLSRGYVRAISQIVGPTKDIPAPDVYTN
SQIMAWMMDEYSRLREFDSPGFITGKPLVLGGSQGRETATAQGVTICIEEAVKKKGIKLQNARIIIQGFGNAGSFLAKFM
HDAGAKVIGISDANGGLYNPDGLDIPYLLDKRDSFGMVTNLFTDVITNEELLEKDCDILVPAAISNQITAKNAHNIQASI
VVEAANGPTTIDATKILNERGVLLVPDILASAGGVTVSYFEWVQNNQGYYWSEEEVAEKLRSVMVSSFETIYQTAATHKV
DMRLAAYMTGIRKSAEASRFRGWV
>Mature_423_residues
SAKQVSKDEEKEALNLFLSTQTIIKEALRKLGYPGDMYELMKEPQRMLTVRIPVKMDNGSVKVFTGYRSQHNDAVGPTKG
GVRFHPEVNEEEVKALSIWMTLKCGIANLPYGGGKGGIICDPRTMSFGELERLSRGYVRAISQIVGPTKDIPAPDVYTNS
QIMAWMMDEYSRLREFDSPGFITGKPLVLGGSQGRETATAQGVTICIEEAVKKKGIKLQNARIIIQGFGNAGSFLAKFMH
DAGAKVIGISDANGGLYNPDGLDIPYLLDKRDSFGMVTNLFTDVITNEELLEKDCDILVPAAISNQITAKNAHNIQASIV
VEAANGPTTIDATKILNERGVLLVPDILASAGGVTVSYFEWVQNNQGYYWSEEEVAEKLRSVMVSSFETIYQTAATHKVD
MRLAAYMTGIRKSAEASRFRGWV

Specific function: Catabolic GlutDH involved in the utilization of glutamate and other amino acids of the glutamate family

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family

Homologues:

Organism=Homo sapiens, GI31377775, Length=338, Percent_Identity=37.8698224852071, Blast_Score=259, Evalue=4e-69,
Organism=Homo sapiens, GI4885281, Length=338, Percent_Identity=37.5739644970414, Blast_Score=256, Evalue=2e-68,
Organism=Escherichia coli, GI1788059, Length=393, Percent_Identity=30.5343511450382, Blast_Score=177, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17544676, Length=340, Percent_Identity=40.8823529411765, Blast_Score=257, Evalue=7e-69,
Organism=Saccharomyces cerevisiae, GI6319256, Length=388, Percent_Identity=29.1237113402062, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6324951, Length=391, Percent_Identity=27.8772378516624, Blast_Score=132, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24649283, Length=328, Percent_Identity=42.0731707317073, Blast_Score=265, Evalue=5e-71,
Organism=Drosophila melanogaster, GI45549226, Length=339, Percent_Identity=38.6430678466077, Blast_Score=258, Evalue=4e-69,
Organism=Drosophila melanogaster, GI45553475, Length=339, Percent_Identity=38.6430678466077, Blast_Score=258, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24649460, Length=279, Percent_Identity=37.9928315412186, Blast_Score=205, Evalue=4e-53,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): DHE2_BACSU (P39633)

Other databases:

- EMBL:   X73124
- EMBL:   AB194695
- EMBL:   AL009126
- EMBL:   S79622
- PIR:   A70055
- RefSeq:   NP_391659.2
- PDB:   3K92
- PDBsum:   3K92
- ProteinModelPortal:   P39633
- SMR:   P39633
- IntAct:   P39633
- EnsemblBacteria:   EBBACT00000001904
- GeneID:   937066
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU37790
- NMPDR:   fig|224308.1.peg.3785
- GenoList:   BSU37790
- GeneTree:   EBGT00050000001222
- HOGENOM:   HBG590661
- PhylomeDB:   P39633
- ProtClustDB:   CLSK888077
- BioCyc:   BSUB:BSU37790-MONOMER
- BRENDA:   1.4.1.2
- GO:   GO:0005488
- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR11606:SF2
- PIRSF:   PIRSF000185
- PRINTS:   PR00082
- SMART:   SM00839

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N

EC number: =1.4.1.2

Molecular weight: Translated: 46554; Mature: 46423

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: ACT_SITE 116-116

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKQVSKDEEKEALNLFLSTQTIIKEALRKLGYPGDMYELMKEPQRMLTVRIPVKMDNG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHEEEEEEEEEECCC
SVKVFTGYRSQHNDAVGPTKGGVRFHPEVNEEEVKALSIWMTLKCGIANLPYGGGKGGII
CEEEEECCCCCCCCCCCCCCCCEEECCCCCHHHHHEEEEEEEEECCCCCCCCCCCCCCEE
CDPRTMSFGELERLSRGYVRAISQIVGPTKDIPAPDVYTNSQIMAWMMDEYSRLREFDSP
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
GFITGKPLVLGGSQGRETATAQGVTICIEEAVKKKGIKLQNARIIIQGFGNAGSFLAKFM
CCCCCCCEEEECCCCCCCHHHCCHHHHHHHHHHHCCCEEECCEEEEEECCCHHHHHHHHH
HDAGAKVIGISDANGGLYNPDGLDIPYLLDKRDSFGMVTNLFTDVITNEELLEKDCDILV
HHCCCEEEEEECCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHCCHHHHHCCCCEEE
PAAISNQITAKNAHNIQASIVVEAANGPTTIDATKILNERGVLLVPDILASAGGVTVSYF
EHHHCCCEECCCCCCEEEEEEEEECCCCCEEHHHHHHCCCCCEEECHHHHCCCCCEEEEE
EWVQNNQGYYWSEEEVAEKLRSVMVSSFETIYQTAATHKVDMRLAAYMTGIRKSAEASRF
HHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGWV
CCCC
>Mature Secondary Structure 
SAKQVSKDEEKEALNLFLSTQTIIKEALRKLGYPGDMYELMKEPQRMLTVRIPVKMDNG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHEEEEEEEEEECCC
SVKVFTGYRSQHNDAVGPTKGGVRFHPEVNEEEVKALSIWMTLKCGIANLPYGGGKGGII
CEEEEECCCCCCCCCCCCCCCCEEECCCCCHHHHHEEEEEEEEECCCCCCCCCCCCCCEE
CDPRTMSFGELERLSRGYVRAISQIVGPTKDIPAPDVYTNSQIMAWMMDEYSRLREFDSP
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
GFITGKPLVLGGSQGRETATAQGVTICIEEAVKKKGIKLQNARIIIQGFGNAGSFLAKFM
CCCCCCCEEEECCCCCCCHHHCCHHHHHHHHHHHCCCEEECCEEEEEECCCHHHHHHHHH
HDAGAKVIGISDANGGLYNPDGLDIPYLLDKRDSFGMVTNLFTDVITNEELLEKDCDILV
HHCCCEEEEEECCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHCCHHHHHCCCCEEE
PAAISNQITAKNAHNIQASIVVEAANGPTTIDATKILNERGVLLVPDILASAGGVTVSYF
EHHHCCCEECCCCCCEEEEEEEEECCCCCEEHHHHHHCCCCCEEECHHHHCCCCCEEEEE
EWVQNNQGYYWSEEEVAEKLRSVMVSSFETIYQTAATHKVDMRLAAYMTGIRKSAEASRF
HHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGWV
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 7565595; 9829940