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The map label for this gene is pbpG

Identifier: 255767792

GI number: 255767792

Start: 3849818

End: 3851893

Strand: Direct

Name: pbpG

Synonym: BSU37510

Alternate gene names: 255767792

Gene position: 3849818-3851893 (Clockwise)

Preceding gene: 16080800

Following gene: 16080806

Centisome position: 91.32

GC content: 50.34

Gene sequence:

>2076_bases
GTGGATGCAATGACAAATAAACGGCTGAGACTGACGCTGAAAACCGTTCGCGCCTTTATCTTTTTAGGGGCTTTTGCGGC
CTTGGCGGCAGCTGCTGTGTTTATGACTGTCATTTTGATAGCCAAATACCAGGGCGCTCCCTCGGTGCAAGTGCCGCAAT
CCACGATTTTGTACGCGAGTGACGGCTCAAAGCTCGGGGAAACGAACTACGGCGAAAAGCGCTATTGGGTCCCCTTAAAG
GACATGAATCCGACGATCGTAAAAGCGACAGTGGCGATTGAAGACCAGAATTTCTACGATCATCACGGCTTTGATTATAA
ACGCATGGCCGGCGCGGCGCTTGCTGACCTGAAAGCATTTGCCAAGGTTCAGGGTGCAAGCACCATCACCCAGCAATATG
CGAGAAACTTATACCTTGAACACGACAAAACGTGGAAACGGAAATGGAATGAGGCGTTCTACACGATCCGTCTGGAACAA
AACTATTCTAAGGACGAGATATTAGAAGGCTATTTAAATACGATTTACTACGGCCACGGCGCCTACGGCATTGAAGCAGC
TTCCCGCCTGTATTTCGGCAAGCATGCCAAAAACCTGACGGATGCGGAGGCGGCGCTTTTGGCAGGAATCCCGAAAGGCC
CTTCCGGCTATTCACCGTATGTCAATGAGACGAAGGCCAAGGAACGCCAGAAAACGATTGTGCGGATGATGGAAAAGCAG
CAGATGATCAGCCAAAAGAAAGCGGATGAATTGATCAAGGAACCTCTTTCCTATCAGCCTTTGAATAAACAAGTTTCCAA
GAGAAAGGCTCCTTATTTCTACGACAATGCGATGAGAGAGCTGGAAAAAAAGCTCGGCATGACACGGGAGCAAATTGAAA
CGAGCGGGTTAAACGTCTATACAACGGTAGATAAACGGATGCAGCGCATCGCCGAGGAGACCATTACCGAAACGGTTAAC
GCGGGCTCTGACATTCAAGTCGGGTTTTCAGCCATTGATCCCCGCACCGGAAACGTTCTCGCCCTTGTCGGCGGCAGGGA
TTATCAAAAAAGCCCGTTTGACCGGACGACACAGGCGAAGCGCCAGCCTGCTTCGACGATCAAGCCGTTGCTGTACTATA
AAGCGATTCAGAGCGGATTTACGCCAGTCACTTTGATGAAAAGCGAAGAAACCGAGTTTCAAATTGATGCAAAGGGAGAA
ACCTATTCTCCAAGCAACTATAACGGATACTATGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAA
TATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCC
ATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGTCCGATTGAGATGGTCAACGCCTATGCCATGCTC
GCAAATGGCGGAAAGAAAATCGAACCGACTTTTATTTCAAGAGTAACGGACGCAGCCGGCCACGTGCTGTACGAAAACCC
GAATCAGCATAAACAGGTGCTTGATGAAAAAGCCGCTTTTGTCACAGCGAGTATGATGACAGGCATGTTTGATATCGATT
TAAATGGATATACGTCGGTTACGGGGCGAACGATAGCCAACCGCCTGACAAGAACGTATGCCGGAAAATCAGGAACCACC
AGCGCAGACAGCTGGATGATCGGCTTCAATCCGAAGCTGGCCGCCGGTGTTTGGACCGGCTATGACAAAAACAGCACCAT
CGACTCGGTCGAAGAAAAAAGCTATGCTAAAACCATTTGGGCGGACTTCATGGAGGACGCGCTCAAAGGCGAACCGGAAA
CCGCGTTCAAGCCGCCAAAAGGCGTAACCGGTGTGTACATTGATCCGGAAACAGGCTACTCATCAGGTCCCGGCTGCGCC
GCTAAGCATTACACATATTTTGTGAAAGGGACAGAACCCGCAAACGTGTGCTACGGGGCAGAACCCGCAAAACAAACAAA
AGACCGGCTTCCATCCAAAGAAAAGCCCGCTTCCGAGAAAAAGTGGTGGGACAAATGGCTGGGGCGACATCATTGA

Upstream 100 bases:

>100_bases
GATGGCGGGGAAAAAGTAAAGATGCGTCCTGTTCTGCGATGTTTAAAAACGATCTTTTTTTCTCATAATAGTAGAAACAT
AAAAAAAGGGGGAACCCGTT

Downstream 100 bases:

>100_bases
AAATAACCCGGCTCCTCGGAGGCCGGGTTATTCGTATGGTTCCTTCATAATCGATGGAAGAATGTTAGACAATCGAGCTG
AACAATCAGTTTTGGGATAA

Product: sporulation specific penicillin-binding protein

Products: NA

Alternate protein names: PBP-2D; PBP2d

Number of amino acids: Translated: 691; Mature: 691

Protein sequence:

>691_residues
MDAMTNKRLRLTLKTVRAFIFLGAFAALAAAAVFMTVILIAKYQGAPSVQVPQSTILYASDGSKLGETNYGEKRYWVPLK
DMNPTIVKATVAIEDQNFYDHHGFDYKRMAGAALADLKAFAKVQGASTITQQYARNLYLEHDKTWKRKWNEAFYTIRLEQ
NYSKDEILEGYLNTIYYGHGAYGIEAASRLYFGKHAKNLTDAEAALLAGIPKGPSGYSPYVNETKAKERQKTIVRMMEKQ
QMISQKKADELIKEPLSYQPLNKQVSKRKAPYFYDNAMRELEKKLGMTREQIETSGLNVYTTVDKRMQRIAEETITETVN
AGSDIQVGFSAIDPRTGNVLALVGGRDYQKSPFDRTTQAKRQPASTIKPLLYYKAIQSGFTPVTLMKSEETEFQIDAKGE
TYSPSNYNGYYANKPITLLQALALSDNIYAVKTHLFLGTNKLVKTAKEFGITAHLQALPSLALGTEPVRPIEMVNAYAML
ANGGKKIEPTFISRVTDAAGHVLYENPNQHKQVLDEKAAFVTASMMTGMFDIDLNGYTSVTGRTIANRLTRTYAGKSGTT
SADSWMIGFNPKLAAGVWTGYDKNSTIDSVEEKSYAKTIWADFMEDALKGEPETAFKPPKGVTGVYIDPETGYSSGPGCA
AKHYTYFVKGTEPANVCYGAEPAKQTKDRLPSKEKPASEKKWWDKWLGRHH

Sequences:

>Translated_691_residues
MDAMTNKRLRLTLKTVRAFIFLGAFAALAAAAVFMTVILIAKYQGAPSVQVPQSTILYASDGSKLGETNYGEKRYWVPLK
DMNPTIVKATVAIEDQNFYDHHGFDYKRMAGAALADLKAFAKVQGASTITQQYARNLYLEHDKTWKRKWNEAFYTIRLEQ
NYSKDEILEGYLNTIYYGHGAYGIEAASRLYFGKHAKNLTDAEAALLAGIPKGPSGYSPYVNETKAKERQKTIVRMMEKQ
QMISQKKADELIKEPLSYQPLNKQVSKRKAPYFYDNAMRELEKKLGMTREQIETSGLNVYTTVDKRMQRIAEETITETVN
AGSDIQVGFSAIDPRTGNVLALVGGRDYQKSPFDRTTQAKRQPASTIKPLLYYKAIQSGFTPVTLMKSEETEFQIDAKGE
TYSPSNYNGYYANKPITLLQALALSDNIYAVKTHLFLGTNKLVKTAKEFGITAHLQALPSLALGTEPVRPIEMVNAYAML
ANGGKKIEPTFISRVTDAAGHVLYENPNQHKQVLDEKAAFVTASMMTGMFDIDLNGYTSVTGRTIANRLTRTYAGKSGTT
SADSWMIGFNPKLAAGVWTGYDKNSTIDSVEEKSYAKTIWADFMEDALKGEPETAFKPPKGVTGVYIDPETGYSSGPGCA
AKHYTYFVKGTEPANVCYGAEPAKQTKDRLPSKEKPASEKKWWDKWLGRHH
>Mature_691_residues
MDAMTNKRLRLTLKTVRAFIFLGAFAALAAAAVFMTVILIAKYQGAPSVQVPQSTILYASDGSKLGETNYGEKRYWVPLK
DMNPTIVKATVAIEDQNFYDHHGFDYKRMAGAALADLKAFAKVQGASTITQQYARNLYLEHDKTWKRKWNEAFYTIRLEQ
NYSKDEILEGYLNTIYYGHGAYGIEAASRLYFGKHAKNLTDAEAALLAGIPKGPSGYSPYVNETKAKERQKTIVRMMEKQ
QMISQKKADELIKEPLSYQPLNKQVSKRKAPYFYDNAMRELEKKLGMTREQIETSGLNVYTTVDKRMQRIAEETITETVN
AGSDIQVGFSAIDPRTGNVLALVGGRDYQKSPFDRTTQAKRQPASTIKPLLYYKAIQSGFTPVTLMKSEETEFQIDAKGE
TYSPSNYNGYYANKPITLLQALALSDNIYAVKTHLFLGTNKLVKTAKEFGITAHLQALPSLALGTEPVRPIEMVNAYAML
ANGGKKIEPTFISRVTDAAGHVLYENPNQHKQVLDEKAAFVTASMMTGMFDIDLNGYTSVTGRTIANRLTRTYAGKSGTT
SADSWMIGFNPKLAAGVWTGYDKNSTIDSVEEKSYAKTIWADFMEDALKGEPETAFKPPKGVTGVYIDPETGYSSGPGCA
AKHYTYFVKGTEPANVCYGAEPAKQTKDRLPSKEKPASEKKWWDKWLGRHH

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family

Homologues:

Organism=Escherichia coli, GI1786343, Length=611, Percent_Identity=29.4599018003273, Blast_Score=231, Evalue=9e-62,
Organism=Escherichia coli, GI87082258, Length=272, Percent_Identity=37.5, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI1788867, Length=580, Percent_Identity=26.2068965517241, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1789601, Length=231, Percent_Identity=33.7662337662338, Blast_Score=118, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PBPG_BACSU (P70997)

Other databases:

- EMBL:   Z80360
- EMBL:   AL009126
- PIR:   F70057
- RefSeq:   NP_391631.2
- ProteinModelPortal:   P70997
- SMR:   P70997
- EnsemblBacteria:   EBBACT00000000222
- GeneID:   937200
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU37510
- NMPDR:   fig|224308.1.peg.3757
- GenoList:   BSU37510
- GeneTree:   EBGT00070000031873
- HOGENOM:   HBG672432
- PhylomeDB:   P70997
- ProtClustDB:   CLSK873707
- BioCyc:   BSUB:BSU37510-MONOMER
- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460
- TIGRFAMs:   TIGR02074

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase; SSF56601 PBP_transp_fold

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 77037; Mature: 77037

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: ACT_SITE 365-365

Signals:

None

Transmembrane regions:

HASH(0x12a38250)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAMTNKRLRLTLKTVRAFIFLGAFAALAAAAVFMTVILIAKYQGAPSVQVPQSTILYAS
CCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEC
DGSKLGETNYGEKRYWVPLKDMNPTIVKATVAIEDQNFYDHHGFDYKRMAGAALADLKAF
CCCCCCCCCCCCCEEEEECCCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
AKVQGASTITQQYARNLYLEHDKTWKRKWNEAFYTIRLEQNYSKDEILEGYLNTIYYGHG
HHHCCHHHHHHHHHHHHEECCCHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHEEECCC
AYGIEAASRLYFGKHAKNLTDAEAALLAGIPKGPSGYSPYVNETKAKERQKTIVRMMEKQ
CHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
QMISQKKADELIKEPLSYQPLNKQVSKRKAPYFYDNAMRELEKKLGMTREQIETSGLNVY
HHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCEEE
TTVDKRMQRIAEETITETVNAGSDIQVGFSAIDPRTGNVLALVGGRDYQKSPFDRTTQAK
EHHHHHHHHHHHHHHHHHHCCCCCEEECCEECCCCCCCEEEEECCCCCCCCCCCHHHHHH
RQPASTIKPLLYYKAIQSGFTPVTLMKSEETEFQIDAKGETYSPSNYNGYYANKPITLLQ
CCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCEECCCHHHHHH
ALALSDNIYAVKTHLFLGTNKLVKTAKEFGITAHLQALPSLALGTEPVRPIEMVNAYAML
HHHHCCCEEEEEEEEEECCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
ANGGKKIEPTFISRVTDAAGHVLYENPNQHKQVLDEKAAFVTASMMTGMFDIDLNGYTSV
HCCCCCCCHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCC
TGRTIANRLTRTYAGKSGTTSADSWMIGFNPKLAAGVWTGYDKNSTIDSVEEKSYAKTIW
HHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHEECCCCCCCCHHHHHHHHHHHHHH
ADFMEDALKGEPETAFKPPKGVTGVYIDPETGYSSGPGCAAKHYTYFVKGTEPANVCYGA
HHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCEEECC
EPAKQTKDRLPSKEKPASEKKWWDKWLGRHH
CHHHHHHHHCCCCCCCCHHHHHHHHHHCCCH
>Mature Secondary Structure
MDAMTNKRLRLTLKTVRAFIFLGAFAALAAAAVFMTVILIAKYQGAPSVQVPQSTILYAS
CCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEC
DGSKLGETNYGEKRYWVPLKDMNPTIVKATVAIEDQNFYDHHGFDYKRMAGAALADLKAF
CCCCCCCCCCCCCEEEEECCCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
AKVQGASTITQQYARNLYLEHDKTWKRKWNEAFYTIRLEQNYSKDEILEGYLNTIYYGHG
HHHCCHHHHHHHHHHHHEECCCHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHEEECCC
AYGIEAASRLYFGKHAKNLTDAEAALLAGIPKGPSGYSPYVNETKAKERQKTIVRMMEKQ
CHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
QMISQKKADELIKEPLSYQPLNKQVSKRKAPYFYDNAMRELEKKLGMTREQIETSGLNVY
HHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCEEE
TTVDKRMQRIAEETITETVNAGSDIQVGFSAIDPRTGNVLALVGGRDYQKSPFDRTTQAK
EHHHHHHHHHHHHHHHHHHCCCCCEEECCEECCCCCCCEEEEECCCCCCCCCCCHHHHHH
RQPASTIKPLLYYKAIQSGFTPVTLMKSEETEFQIDAKGETYSPSNYNGYYANKPITLLQ
CCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCEECCCHHHHHH
ALALSDNIYAVKTHLFLGTNKLVKTAKEFGITAHLQALPSLALGTEPVRPIEMVNAYAML
HHHHCCCEEEEEEEEEECCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
ANGGKKIEPTFISRVTDAAGHVLYENPNQHKQVLDEKAAFVTASMMTGMFDIDLNGYTSV
HCCCCCCCHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCC
TGRTIANRLTRTYAGKSGTTSADSWMIGFNPKLAAGVWTGYDKNSTIDSVEEKSYAKTIW
HHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHEECCCCCCCCHHHHHHHHHHHHHH
ADFMEDALKGEPETAFKPPKGVTGVYIDPETGYSSGPGCAAKHYTYFVKGTEPANVCYGA
HHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCEEECC
EPAKQTKDRLPSKEKPASEKKWWDKWLGRHH
CHHHHHHHHCCCCCCCCHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377