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The map label for this gene is rho

Identifier: 255767787

GI number: 255767787

Start: 3803400

End: 3804683

Strand: Reverse

Name: rho

Synonym: BSU37080

Alternate gene names: 255767787

Gene position: 3804683-3803400 (Counterclockwise)

Preceding gene: 16080762

Following gene: 16080760

Centisome position: 90.25

GC content: 46.57

Gene sequence:

>1284_bases
ATGAAAGACGTATCTATTTCCTCTTTGGAAAATATGAAATTGAAAGAGCTTTATGAACTTGCAAGACATTATAAAATCTC
CTATTACAGCAAACTGACAAAAAAAGAACTCATTTTCGCCATTCTGAAAGCGAATGCAGAACAGGAAGATCTGCTGTTTA
TGGAAGGCGTTCTCGAGATCATCCAGTCTGAAGGTTTCGGATTCCTGAGACCGATCAACTACTCTCCAAGCTCAGAAGAC
ATTTACATCTCAGCTTCACAAATCCGCCGTTTCGATTTGCGGAACGGAGACAAAGTATCTGGCAAGGTTCGCCCGCCAAA
AGAAAATGAGCGTTACTATGGACTTTTGCACGTTGAAGCAGTAAATGGGGATGATCCCGAATCTGCAAAAGAGCGTGTGC
ATTTCCCGGCTCTTACGCCACTTTATCCGGATCGTCAAATGGTGCTTGAAACAAAGCCGAACTTCTTGTCTACAAGAATT
ATGGACATGATGGCGCCGGTTGGATTTGGGCAGCGCGGATTGATTGTTGCGCCGCCGAAAGCCGGAAAAACGATGTTGCT
GAAGGAAATTGCCAACAGCATTACAGCGAACCAGCCTGAAGCAGAGCTGATCGTGCTTTTAATTGACGAAAGACCTGAGG
AAGTAACCGATATCGAGCGCTCTGTAGCTGGGGATGTCGTCAGCTCAACGTTTGATGAAGTGCCGGAAAACCATATCAAA
GTGGCCGAGCTTGTGCTTGAACGTGCGATGCGTCTCGTGGAACACAAAAAAGACGTCATTATCCTGATGGACAGCATCAC
ACGTCTTGCCCGCGCCTACAACTTAGTGATTCCGCCAAGTGGAAGAACGCTTTCCGGGGGGATTGACCCAGCGGCGTTCC
ACCGTCCGAAACGCTTCTTTGGGGCTGCGAGAAATATCGAAGAGGGCGGCAGCTTAACCATCCTTGCTACGGCTCTGGTC
GATACAGGTTCACGTATGGATGATGTCATTTATGAAGAATTCAAGGGAACAGGCAACATGGAGCTCCATCTTGACCGCTC
TCTTGCCGAGCGCCGCATCTTCCCTGCCATCGATATCCGCCGTTCAGGAACGCGCAAAGAAGAGCTGCTTGTGCCTAAAG
AGCATCTTGATCGTTTATGGTCTATCCGCAAAACGATGTCTGATTCACCTGATTTCGCAGAAAAGTTCATGAGAAAAATG
AAAAAAACCAAAACAAACCAGGAATTTTTCGATATTCTCAATCAAGAATGGAAACAGGCAAATCTATCATCTGCAAGAAG
GTAA

Upstream 100 bases:

>100_bases
CTTTATGTTAAAAAAAGAATGTGCTTTGGCGGATGTTGTGCAAAAGCAGCAGATGACATGTTCAGAGTAAGATATTGACT
ATGAAAAGCGTGGTGTTTTT

Downstream 100 bases:

>100_bases
ATTGTATTTGCAAAAGAAGTAAATCACTGATATAATTTTCTCATATGCGTTTCGGACAATGCTCCGAAATAATAACTCTG
TTTCCACACGGGATTCAGGG

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKDVSISSLENMKLKELYELARHYKISYYSKLTKKELIFAILKANAEQEDLLFMEGVLEIIQSEGFGFLRPINYSPSSED
IYISASQIRRFDLRNGDKVSGKVRPPKENERYYGLLHVEAVNGDDPESAKERVHFPALTPLYPDRQMVLETKPNFLSTRI
MDMMAPVGFGQRGLIVAPPKAGKTMLLKEIANSITANQPEAELIVLLIDERPEEVTDIERSVAGDVVSSTFDEVPENHIK
VAELVLERAMRLVEHKKDVIILMDSITRLARAYNLVIPPSGRTLSGGIDPAAFHRPKRFFGAARNIEEGGSLTILATALV
DTGSRMDDVIYEEFKGTGNMELHLDRSLAERRIFPAIDIRRSGTRKEELLVPKEHLDRLWSIRKTMSDSPDFAEKFMRKM
KKTKTNQEFFDILNQEWKQANLSSARR

Sequences:

>Translated_427_residues
MKDVSISSLENMKLKELYELARHYKISYYSKLTKKELIFAILKANAEQEDLLFMEGVLEIIQSEGFGFLRPINYSPSSED
IYISASQIRRFDLRNGDKVSGKVRPPKENERYYGLLHVEAVNGDDPESAKERVHFPALTPLYPDRQMVLETKPNFLSTRI
MDMMAPVGFGQRGLIVAPPKAGKTMLLKEIANSITANQPEAELIVLLIDERPEEVTDIERSVAGDVVSSTFDEVPENHIK
VAELVLERAMRLVEHKKDVIILMDSITRLARAYNLVIPPSGRTLSGGIDPAAFHRPKRFFGAARNIEEGGSLTILATALV
DTGSRMDDVIYEEFKGTGNMELHLDRSLAERRIFPAIDIRRSGTRKEELLVPKEHLDRLWSIRKTMSDSPDFAEKFMRKM
KKTKTNQEFFDILNQEWKQANLSSARR
>Mature_427_residues
MKDVSISSLENMKLKELYELARHYKISYYSKLTKKELIFAILKANAEQEDLLFMEGVLEIIQSEGFGFLRPINYSPSSED
IYISASQIRRFDLRNGDKVSGKVRPPKENERYYGLLHVEAVNGDDPESAKERVHFPALTPLYPDRQMVLETKPNFLSTRI
MDMMAPVGFGQRGLIVAPPKAGKTMLLKEIANSITANQPEAELIVLLIDERPEEVTDIERSVAGDVVSSTFDEVPENHIK
VAELVLERAMRLVEHKKDVIILMDSITRLARAYNLVIPPSGRTLSGGIDPAAFHRPKRFFGAARNIEEGGSLTILATALV
DTGSRMDDVIYEEFKGTGNMELHLDRSLAERRIFPAIDIRRSGTRKEELLVPKEHLDRLWSIRKTMSDSPDFAEKFMRKM
KKTKTNQEFFDILNQEWKQANLSSARR

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family

Homologues:

Organism=Escherichia coli, GI1790217, Length=419, Percent_Identity=56.563245823389, Blast_Score=485, Evalue=1e-138,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): RHO_BACSU (Q03222)

Other databases:

- EMBL:   M97678
- EMBL:   Z49782
- EMBL:   AL009126
- PIR:   A47051
- RefSeq:   NP_391589.2
- ProteinModelPortal:   Q03222
- SMR:   Q03222
- EnsemblBacteria:   EBBACT00000003645
- GeneID:   937042
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU37080
- NMPDR:   fig|224308.1.peg.3715
- GenoList:   BSU37080
- GeneTree:   EBGT00050000001319
- HOGENOM:   HBG335262
- PhylomeDB:   Q03222
- ProtClustDB:   PRK09376
- BioCyc:   BSUB:BSU37080-MONOMER
- HAMAP:   MF_01884
- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00382
- SMART:   SM00357
- SMART:   SM00959
- TIGRFAMs:   TIGR00767

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind; SSF68912 Rho_N

EC number: NA

Molecular weight: Translated: 48630; Mature: 48630

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: BINDING 211-211

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDVSISSLENMKLKELYELARHYKISYYSKLTKKELIFAILKANAEQEDLLFMEGVLEI
CCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
IQSEGFGFLRPINYSPSSEDIYISASQIRRFDLRNGDKVSGKVRPPKENERYYGLLHVEA
HHHCCCCEEEECCCCCCCCCEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEE
VNGDDPESAKERVHFPALTPLYPDRQMVLETKPNFLSTRIMDMMAPVGFGQRGLIVAPPK
ECCCCCHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCEEEECCC
AGKTMLLKEIANSITANQPEAELIVLLIDERPEEVTDIERSVAGDVVSSTFDEVPENHIK
CCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VAELVLERAMRLVEHKKDVIILMDSITRLARAYNLVIPPSGRTLSGGIDPAAFHRPKRFF
HHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHEEEECCCCCEECCCCCHHHHHCCHHHH
GAARNIEEGGSLTILATALVDTGSRMDDVIYEEFKGTGNMELHLDRSLAERRIFPAIDIR
HHHCCCCCCCCEEEEEEEHHHCCCHHHHHHHHHHCCCCCEEEEECHHHHHHHCCCCEECC
RSGTRKEELLVPKEHLDRLWSIRKTMSDSPDFAEKFMRKMKKTKTNQEFFDILNQEWKQA
CCCCCHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
NLSSARR
CHHHCCC
>Mature Secondary Structure
MKDVSISSLENMKLKELYELARHYKISYYSKLTKKELIFAILKANAEQEDLLFMEGVLEI
CCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
IQSEGFGFLRPINYSPSSEDIYISASQIRRFDLRNGDKVSGKVRPPKENERYYGLLHVEA
HHHCCCCEEEECCCCCCCCCEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEE
VNGDDPESAKERVHFPALTPLYPDRQMVLETKPNFLSTRIMDMMAPVGFGQRGLIVAPPK
ECCCCCHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCEEEECCC
AGKTMLLKEIANSITANQPEAELIVLLIDERPEEVTDIERSVAGDVVSSTFDEVPENHIK
CCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VAELVLERAMRLVEHKKDVIILMDSITRLARAYNLVIPPSGRTLSGGIDPAAFHRPKRFF
HHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHEEEECCCCCEECCCCCHHHHHCCHHHH
GAARNIEEGGSLTILATALVDTGSRMDDVIYEEFKGTGNMELHLDRSLAERRIFPAIDIR
HHHCCCCCCCCEEEEEEEHHHCCCHHHHHHHHHHCCCCCEEEEECHHHHHHHCCCCEECC
RSGTRKEELLVPKEHLDRLWSIRKTMSDSPDFAEKFMRKMKKTKTNQEFFDILNQEWKQA
CCCCCHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
NLSSARR
CHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423140; 9353933; 9384377