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The map label for this gene is ssbB

Identifier: 255767782

GI number: 255767782

Start: 3740206

End: 3740547

Strand: Reverse

Name: ssbB

Synonym: BSU36310

Alternate gene names: 255767782

Gene position: 3740547-3740206 (Counterclockwise)

Preceding gene: 16080685

Following gene: 16080683

Centisome position: 88.73

GC content: 47.66

Gene sequence:

>342_bases
ATGTTCAATCAGGTCATGCTTGTCGGACGTCTTACAAAAGATCCTGATCTTCGCTACACTTCCGCCGGTGCGGCAGTTGC
ACATGTTACGCTCGCGGTGAACCGCAGCTTCAAGAATGCTTCAGGTGAAATCGAAGCTGATTACGTCAATTGCACACTTT
GGAGAAAAACAGCTGAAAACACGGCGTTGTATTGCCAAAAAGGTTCTCTCGTCGGCGTAAGCGGACGGATTCAGACAAGA
AGCTATGAAAACGAGGAAGGCGTTAACGTGTATGTAACAGAAGTGTTGGCTGACACTGTTCGTTTTATGGACCCTAAACC
CCGGGAAAAAGCTGCTGATTGA

Upstream 100 bases:

>100_bases
ACGAAAGATAAAAACAGAGGCTGAAAGCCATTTTTAAGCGTTTTTCTTTTCTTGTTGCATCATTTACAATACATACAACC
GCAAGGAGAGGAGGAATCGC

Downstream 100 bases:

>100_bases
CACCCCTGCTCCTCCCGTTATCTATCCATTCACTATTATCCGGATCTTCGCATCATCCCCTCAAAATTGACCAGTCGACC
AGACTGGTTCTTTCTTTTTG

Product: single-strand DNA-binding protein

Products: NA

Alternate protein names: SSB

Number of amino acids: Translated: 113; Mature: 113

Protein sequence:

>113_residues
MFNQVMLVGRLTKDPDLRYTSAGAAVAHVTLAVNRSFKNASGEIEADYVNCTLWRKTAENTALYCQKGSLVGVSGRIQTR
SYENEEGVNVYVTEVLADTVRFMDPKPREKAAD

Sequences:

>Translated_113_residues
MFNQVMLVGRLTKDPDLRYTSAGAAVAHVTLAVNRSFKNASGEIEADYVNCTLWRKTAENTALYCQKGSLVGVSGRIQTR
SYENEEGVNVYVTEVLADTVRFMDPKPREKAAD
>Mature_113_residues
MFNQVMLVGRLTKDPDLRYTSAGAAVAHVTLAVNRSFKNASGEIEADYVNCTLWRKTAENTALYCQKGSLVGVSGRIQTR
SYENEEGVNVYVTEVLADTVRFMDPKPREKAAD

Specific function: Not essential for replication of the chromosome but is required for optimal competence. Probably binds ssDNA and allows DNA recombination

COG id: COG0629

COG function: function code L; Single-stranded DNA-binding protein

Gene ontology:

Cell location: Cytoplasm. Note=Localizes to the cell poles during the development of competence

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SSB domain

Homologues:

Organism=Escherichia coli, GI1790494, Length=99, Percent_Identity=38.3838383838384, Blast_Score=74, Evalue=3e-15,

Paralogues:

None

Copy number: 1622 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SSB2_BACSU (C0SPB6)

Other databases:

- EMBL:   Z83337
- EMBL:   AL009126
- PIR:   B70066
- RefSeq:   NP_391512.2
- ProteinModelPortal:   C0SPB6
- SMR:   C0SPB6
- EnsemblBacteria:   EBBACT00000000149
- GeneID:   936910
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU36310
- NMPDR:   fig|224308.1.peg.3638
- GenoList:   BSU36310
- GeneTree:   EBGT00050000001256
- ProtClustDB:   CLSK2793894
- BioCyc:   BSUB:BSU36310-MONOMER
- GO:   GO:0005737
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000424
- InterPro:   IPR011344
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR10302
- TIGRFAMs:   TIGR00621

Pfam domain/function: PF00436 SSB; SSF50249 Nucleic_acid_OB

EC number: NA

Molecular weight: Translated: 12520; Mature: 12520

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS50935 SSB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNQVMLVGRLTKDPDLRYTSAGAAVAHVTLAVNRSFKNASGEIEADYVNCTLWRKTAEN
CCCCEEEEEECCCCCCCEEECCCCEEEEEEEEEECCCCCCCCCEEEEEEEEEEEECCCCC
TALYCQKGSLVGVSGRIQTRSYENEEGVNVYVTEVLADTVRFMDPKPREKAAD
CEEEEECCCEEEECCEEEECCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MFNQVMLVGRLTKDPDLRYTSAGAAVAHVTLAVNRSFKNASGEIEADYVNCTLWRKTAEN
CCCCEEEEEECCCCCCCEEECCCCEEEEEEEEEECCCCCCCCCEEEEEEEEEEEECCCCC
TALYCQKGSLVGVSGRIQTRSYENEEGVNVYVTEVLADTVRFMDPKPREKAAD
CEEEEECCCEEEECCEEEECCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377