Definition Bacillus subtilis subsp. subtilis str. 168 chromosome, complete genome.
Accession NC_000964
Length 4,215,606

Click here to switch to the map view.

The map label for this gene is alsS

Identifier: 255767778

GI number: 255767778

Start: 3709628

End: 3711340

Strand: Reverse

Name: alsS

Synonym: BSU36010

Alternate gene names: 255767778

Gene position: 3711340-3709628 (Counterclockwise)

Preceding gene: 16080657

Following gene: 16080653

Centisome position: 88.04

GC content: 45.71

Gene sequence:

>1713_bases
TTGACAAAAGCAACAAAAGAACAAAAATCCCTTGTGAAAAACAGAGGGGCGGAGCTTGTTGTTGATTGCTTAGTGGAGCA
AGGTGTCACACATGTATTTGGCATTCCAGGTGCAAAAATTGATGCGGTATTTGACGCTTTACAAGATAAAGGACCTGAAA
TTATCGTTGCCCGGCACGAACAAAACGCAGCATTCATGGCCCAAGCAGTCGGCCGTTTAACTGGAAAACCGGGAGTCGTG
TTAGTCACATCAGGACCGGGTGCCTCTAACTTGGCAACAGGCCTGCTGACAGCGAACACTGAAGGAGACCCTGTCGTTGC
GCTTGCTGGAAACGTGATCCGTGCAGATCGTTTAAAACGGACACATCAATCTTTGGATAATGCGGCGCTATTCCAGCCGA
TTACAAAATACAGTGTAGAAGTTCAAGATGTAAAAAATATACCGGAAGCTGTTACAAATGCATTTAGGATAGCGTCAGCA
GGGCAGGCTGGGGCCGCTTTTGTGAGCTTTCCGCAAGATGTTGTGAATGAAGTCACAAATACGAAAAACGTGCGTGCTGT
TGCAGCGCCAAAACTCGGTCCTGCAGCAGATGATGCAATCAGTGCGGCCATAGCAAAAATCCAAACAGCAAAACTTCCTG
TCGTTTTGGTCGGCATGAAAGGCGGAAGACCGGAAGCAATTAAAGCGGTTCGCAAGCTTTTGAAAAAGGTTCAGCTTCCA
TTTGTTGAAACATATCAAGCTGCCGGTACCCTTTCTAGAGATTTAGAGGATCAATATTTTGGCCGTATCGGTTTGTTCCG
CAACCAGCCTGGCGATTTACTGCTAGAGCAGGCAGATGTTGTTCTGACGATCGGCTATGACCCGATTGAATATGATCCGA
AATTCTGGAATATCAATGGAGACCGGACAATTATCCATTTAGACGAGATTATCGCTGACATTGATCATGCTTACCAGCCT
GATCTTGAATTGATCGGTGACATTCCGTCCACGATCAATCATATCGAACACGATGCTGTGAAAGTGGAATTTGCAGAGCG
TGAGCAGAAAATCCTTTCTGATTTAAAACAATATATGCATGAAGGTGAGCAGGTGCCTGCAGATTGGAAATCAGACAGAG
CGCACCCTCTTGAAATCGTTAAAGAGTTGCGTAATGCAGTCGATGATCATGTTACAGTAACTTGCGATATCGGTTCGCAC
GCCATTTGGATGTCACGTTATTTCCGCAGCTACGAGCCGTTAACATTAATGATCAGTAACGGTATGCAAACACTCGGCGT
TGCGCTTCCTTGGGCAATCGGCGCTTCATTGGTGAAACCGGGAGAAAAAGTGGTTTCTGTCTCTGGTGACGGCGGTTTCT
TATTCTCAGCAATGGAATTAGAGACAGCAGTTCGACTAAAAGCACCAATTGTACACATTGTATGGAACGACAGCACATAT
GACATGGTTGCATTCCAGCAATTGAAAAAATATAACCGTACATCTGCGGTCGATTTCGGAAATATCGATATCGTGAAATA
TGCGGAAAGCTTCGGAGCAACTGGCTTGCGCGTAGAATCACCAGACCAGCTGGCAGATGTTCTGCGTCAAGGCATGAACG
CTGAAGGTCCTGTCATCATCGATGTCCCGGTTGACTACAGTGATAACATTAATTTAGCAAGTGACAAGCTTCCGAAAGAA
TTCGGGGAACTCATGAAAACGAAAGCTCTCTAG

Upstream 100 bases:

>100_bases
GAATCGATATTGGAGGTCAATTTCCAAAGAGTGTATAGTGAAACTTATCACAAGATATTTAAAATTTTACGTTTAAAATG
CATAATAAGGAGTGAGGGTG

Downstream 100 bases:

>100_bases
CACTCTGCGCATCACGACACTGTTTTATGAACAGCACTAAATAAAAGGAGTGAAGGGAAATATGAAACGAGAAAGCAACA
TTCAAGTGCTCAGCCGTGGT

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase

Number of amino acids: Translated: 570; Mature: 569

Protein sequence:

>570_residues
MTKATKEQKSLVKNRGAELVVDCLVEQGVTHVFGIPGAKIDAVFDALQDKGPEIIVARHEQNAAFMAQAVGRLTGKPGVV
LVTSGPGASNLATGLLTANTEGDPVVALAGNVIRADRLKRTHQSLDNAALFQPITKYSVEVQDVKNIPEAVTNAFRIASA
GQAGAAFVSFPQDVVNEVTNTKNVRAVAAPKLGPAADDAISAAIAKIQTAKLPVVLVGMKGGRPEAIKAVRKLLKKVQLP
FVETYQAAGTLSRDLEDQYFGRIGLFRNQPGDLLLEQADVVLTIGYDPIEYDPKFWNINGDRTIIHLDEIIADIDHAYQP
DLELIGDIPSTINHIEHDAVKVEFAEREQKILSDLKQYMHEGEQVPADWKSDRAHPLEIVKELRNAVDDHVTVTCDIGSH
AIWMSRYFRSYEPLTLMISNGMQTLGVALPWAIGASLVKPGEKVVSVSGDGGFLFSAMELETAVRLKAPIVHIVWNDSTY
DMVAFQQLKKYNRTSAVDFGNIDIVKYAESFGATGLRVESPDQLADVLRQGMNAEGPVIIDVPVDYSDNINLASDKLPKE
FGELMKTKAL

Sequences:

>Translated_570_residues
MTKATKEQKSLVKNRGAELVVDCLVEQGVTHVFGIPGAKIDAVFDALQDKGPEIIVARHEQNAAFMAQAVGRLTGKPGVV
LVTSGPGASNLATGLLTANTEGDPVVALAGNVIRADRLKRTHQSLDNAALFQPITKYSVEVQDVKNIPEAVTNAFRIASA
GQAGAAFVSFPQDVVNEVTNTKNVRAVAAPKLGPAADDAISAAIAKIQTAKLPVVLVGMKGGRPEAIKAVRKLLKKVQLP
FVETYQAAGTLSRDLEDQYFGRIGLFRNQPGDLLLEQADVVLTIGYDPIEYDPKFWNINGDRTIIHLDEIIADIDHAYQP
DLELIGDIPSTINHIEHDAVKVEFAEREQKILSDLKQYMHEGEQVPADWKSDRAHPLEIVKELRNAVDDHVTVTCDIGSH
AIWMSRYFRSYEPLTLMISNGMQTLGVALPWAIGASLVKPGEKVVSVSGDGGFLFSAMELETAVRLKAPIVHIVWNDSTY
DMVAFQQLKKYNRTSAVDFGNIDIVKYAESFGATGLRVESPDQLADVLRQGMNAEGPVIIDVPVDYSDNINLASDKLPKE
FGELMKTKAL
>Mature_569_residues
TKATKEQKSLVKNRGAELVVDCLVEQGVTHVFGIPGAKIDAVFDALQDKGPEIIVARHEQNAAFMAQAVGRLTGKPGVVL
VTSGPGASNLATGLLTANTEGDPVVALAGNVIRADRLKRTHQSLDNAALFQPITKYSVEVQDVKNIPEAVTNAFRIASAG
QAGAAFVSFPQDVVNEVTNTKNVRAVAAPKLGPAADDAISAAIAKIQTAKLPVVLVGMKGGRPEAIKAVRKLLKKVQLPF
VETYQAAGTLSRDLEDQYFGRIGLFRNQPGDLLLEQADVVLTIGYDPIEYDPKFWNINGDRTIIHLDEIIADIDHAYQPD
LELIGDIPSTINHIEHDAVKVEFAEREQKILSDLKQYMHEGEQVPADWKSDRAHPLEIVKELRNAVDDHVTVTCDIGSHA
IWMSRYFRSYEPLTLMISNGMQTLGVALPWAIGASLVKPGEKVVSVSGDGGFLFSAMELETAVRLKAPIVHIVWNDSTYD
MVAFQQLKKYNRTSAVDFGNIDIVKYAESFGATGLRVESPDQLADVLRQGMNAEGPVIIDVPVDYSDNINLASDKLPKEF
GELMKTKAL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family

Homologues:

Organism=Homo sapiens, GI93004078, Length=562, Percent_Identity=25.9786476868327, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI21361361, Length=597, Percent_Identity=23.1155778894472, Blast_Score=146, Evalue=4e-35,
Organism=Escherichia coli, GI87081685, Length=556, Percent_Identity=28.0575539568345, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1790104, Length=543, Percent_Identity=29.6500920810313, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1786717, Length=574, Percent_Identity=25.609756097561, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1787096, Length=538, Percent_Identity=25.4646840148699, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1788716, Length=557, Percent_Identity=25.673249551167, Blast_Score=137, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17542570, Length=546, Percent_Identity=24.7252747252747, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17531299, Length=553, Percent_Identity=26.5822784810127, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17531301, Length=553, Percent_Identity=26.5822784810127, Blast_Score=130, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6323755, Length=568, Percent_Identity=29.4014084507042, Blast_Score=223, Evalue=9e-59,
Organism=Saccharomyces cerevisiae, GI6320816, Length=475, Percent_Identity=24.8421052631579, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19922626, Length=582, Percent_Identity=27.319587628866, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVX_BACSU (Q04789)

Other databases:

- EMBL:   L04470
- EMBL:   Z93767
- EMBL:   AL009126
- PIR:   H69584
- RefSeq:   NP_391482.2
- ProteinModelPortal:   Q04789
- SMR:   Q04789
- EnsemblBacteria:   EBBACT00000000610
- GeneID:   936852
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU36010
- NMPDR:   fig|224308.1.peg.3608
- GenoList:   BSU36010
- GeneTree:   EBGT00070000031807
- HOGENOM:   HBG323037
- ProtClustDB:   PRK08617
- BioCyc:   BSUB:BSU36010-MONOMER
- BRENDA:   2.2.1.6
- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766
- TIGRFAMs:   TIGR02418

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N

EC number: =2.2.1.6

Molecular weight: Translated: 62005; Mature: 61873

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00187 TPP_ENZYMES

Important sites: BINDING 60-60 BINDING 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MTKATKEQKSLVKNRGAELVVDCLVEQGVTHVFGIPGAKIDAVFDALQDKGPEIIVARHE
CCCCCHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCCEEEEEECC
QNAAFMAQAVGRLTGKPGVVLVTSGPGASNLATGLLTANTEGDPVVALAGNVIRADRLKR
CCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHEEEEECCCCCEEEEECCCCHHHHHHHH
THQSLDNAALFQPITKYSVEVQDVKNIPEAVTNAFRIASAGQAGAAFVSFPQDVVNEVTN
HHHHHCHHHHHHHHHHHEEEHHHHHCCHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHCC
TKNVRAVAAPKLGPAADDAISAAIAKIQTAKLPVVLVGMKGGRPEAIKAVRKLLKKVQLP
CCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCC
FVETYQAAGTLSRDLEDQYFGRIGLFRNQPGDLLLEQADVVLTIGYDPIEYDPKFWNING
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCEEEEECCCCCCCCCCEEECCC
DRTIIHLDEIIADIDHAYQPDLELIGDIPSTINHIEHDAVKVEFAEREQKILSDLKQYMH
CEEEEEHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHH
EGEQVPADWKSDRAHPLEIVKELRNAVDDHVTVTCDIGSHAIWMSRYFRSYEPLTLMISN
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCCEEEEECC
GMQTLGVALPWAIGASLVKPGEKVVSVSGDGGFLFSAMELETAVRLKAPIVHIVWNDSTY
CCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEHHHHHHHHEEECCEEEEEECCCCH
DMVAFQQLKKYNRTSAVDFGNIDIVKYAESFGATGLRVESPDQLADVLRQGMNAEGPVII
HHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHCCCCCEECCHHHHHHHHHHCCCCCCCEEE
DVPVDYSDNINLASDKLPKEFGELMKTKAL
EECCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKATKEQKSLVKNRGAELVVDCLVEQGVTHVFGIPGAKIDAVFDALQDKGPEIIVARHE
CCCCHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCCEEEEEECC
QNAAFMAQAVGRLTGKPGVVLVTSGPGASNLATGLLTANTEGDPVVALAGNVIRADRLKR
CCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHEEEEECCCCCEEEEECCCCHHHHHHHH
THQSLDNAALFQPITKYSVEVQDVKNIPEAVTNAFRIASAGQAGAAFVSFPQDVVNEVTN
HHHHHCHHHHHHHHHHHEEEHHHHHCCHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHCC
TKNVRAVAAPKLGPAADDAISAAIAKIQTAKLPVVLVGMKGGRPEAIKAVRKLLKKVQLP
CCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCC
FVETYQAAGTLSRDLEDQYFGRIGLFRNQPGDLLLEQADVVLTIGYDPIEYDPKFWNING
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCEEEEECCCCCCCCCCEEECCC
DRTIIHLDEIIADIDHAYQPDLELIGDIPSTINHIEHDAVKVEFAEREQKILSDLKQYMH
CEEEEEHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHH
EGEQVPADWKSDRAHPLEIVKELRNAVDDHVTVTCDIGSHAIWMSRYFRSYEPLTLMISN
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCCEEEEECC
GMQTLGVALPWAIGASLVKPGEKVVSVSGDGGFLFSAMELETAVRLKAPIVHIVWNDSTY
CCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEHHHHHHHHEEECCEEEEEECCCCH
DMVAFQQLKKYNRTSAVDFGNIDIVKYAESFGATGLRVESPDQLADVLRQGMNAEGPVII
HHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHCCCCCEECCHHHHHHHHHHCCCCCCCEEE
DVPVDYSDNINLASDKLPKEFGELMKTKAL
EECCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377; 10809684