Click here to switch to the map view.

The map label for this gene is besA

Identifier: 255767735

GI number: 255767735

Start: 3292490

End: 3293359

Strand: Reverse

Name: besA

Synonym: BSU32010

Alternate gene names: 255767735

Gene position: 3293359-3292490 (Counterclockwise)

Preceding gene: 16080255

Following gene: 255767734

Centisome position: 78.12

GC content: 48.97

Gene sequence:

>870_bases
ATGAAAGAACAAACAACAGACCGGACAAATGGCGGGACATCAAACGCATTTACGATTCCTGGTACGGAAGTGCGTATGAT
GTCTTCACGCAATGAAAACCGTACCTATCACATTTTTATCTCGAAACCTAGTACGCCGCCGCCACCCGCTGGTTATCCTG
TGATTTATCTGCTGGATGCCAATTCTGTCTTCGGAACGATGACGGAAGCCGTTCGAATACAGGGACGCCGACCCGAAAAA
ACCGGGGTGATCCCTGCAGTGATCGTCGGCATTGGCTATGAGACAGCGGAGCCGTTCTCCTCAGCACGCCACCGGGATTT
TACAATGCCAACAGCCCAATCGAAGCTGCCGGAAAGGCCCGACGGCAGAGAATGGCCGGAGCATGGCGGAGCGGAGGGCT
TTTTCAGATTTATTGAAGAGGATTTAAAACCGGAGATAGAACGAGATTACCAGATTGATAAAAAAAGACAAACAATCTTT
GGCCATTCGCTCGGAGGGCTTTTCGTGCTGCAGGTGCTGCTAACAAAGCCCGATGCGTTTCAAACGTATATCGCGGGAAG
TCCGTCCATTCATTGGAATAAACCGTTTATTCTCAAGAAAACGGACCATTTCGTCTCTCTCACCAAGAAAAACAATCAGC
CTATCAATATCCTGCTTGCCGCAGGAGAATTAGAGCAGCATCATAAAAGCCGGATGAATGATAATGCAAGGGAGCTTTAT
GAGCGGCTGGCCGTTTTATCTGAACAAGGTATACGGGCAGAATTTTGCGAGTTTTCAGGAGAAGGACATATTTCAGTTCT
TCCTGTTTTGGTCAGCAGGGCGCTGCGTTTTGCGCTTCACCCTGACGGACCGCATCTATCAATGGGCTGA

Upstream 100 bases:

>100_bases
CTTTCTTTGGAAGAAAACCTATTATTTGAGGTGTCTAGTTGATAGTGAAAATCATTATCATACATTGCGGTTTAGTTTAA
GGAGAGGAATGTCGTACTGC

Downstream 100 bases:

>100_bases
GACATACTCAGCCTTGCCTTTAAAAAAATATTTGTCACTGAAATTATATTTGACTGTCACATGACATTTGGATATGATTA
TTTTTATAATTGATAATGAT

Product: bacillibactin trilactone hydrolase

Products: NA

Alternate protein names: Bacillibactin trilactone hydrolase

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MKEQTTDRTNGGTSNAFTIPGTEVRMMSSRNENRTYHIFISKPSTPPPPAGYPVIYLLDANSVFGTMTEAVRIQGRRPEK
TGVIPAVIVGIGYETAEPFSSARHRDFTMPTAQSKLPERPDGREWPEHGGAEGFFRFIEEDLKPEIERDYQIDKKRQTIF
GHSLGGLFVLQVLLTKPDAFQTYIAGSPSIHWNKPFILKKTDHFVSLTKKNNQPINILLAAGELEQHHKSRMNDNARELY
ERLAVLSEQGIRAEFCEFSGEGHISVLPVLVSRALRFALHPDGPHLSMG

Sequences:

>Translated_289_residues
MKEQTTDRTNGGTSNAFTIPGTEVRMMSSRNENRTYHIFISKPSTPPPPAGYPVIYLLDANSVFGTMTEAVRIQGRRPEK
TGVIPAVIVGIGYETAEPFSSARHRDFTMPTAQSKLPERPDGREWPEHGGAEGFFRFIEEDLKPEIERDYQIDKKRQTIF
GHSLGGLFVLQVLLTKPDAFQTYIAGSPSIHWNKPFILKKTDHFVSLTKKNNQPINILLAAGELEQHHKSRMNDNARELY
ERLAVLSEQGIRAEFCEFSGEGHISVLPVLVSRALRFALHPDGPHLSMG
>Mature_289_residues
MKEQTTDRTNGGTSNAFTIPGTEVRMMSSRNENRTYHIFISKPSTPPPPAGYPVIYLLDANSVFGTMTEAVRIQGRRPEK
TGVIPAVIVGIGYETAEPFSSARHRDFTMPTAQSKLPERPDGREWPEHGGAEGFFRFIEEDLKPEIERDYQIDKKRQTIF
GHSLGGLFVLQVLLTKPDAFQTYIAGSPSIHWNKPFILKKTDHFVSLTKKNNQPINILLAAGELEQHHKSRMNDNARELY
ERLAVLSEQGIRAEFCEFSGEGHISVLPVLVSRALRFALHPDGPHLSMG

Specific function: Catalyzes the hydrolysis of the trilactone cycle of ferri-bacillibactin (ferri-BB) complex, leading to the formation of bacillibactin monomers and to cytosolic iron release, thus making iron available for metabolic use. Can also hydrolyze bacillibactin (B

COG id: COG2819

COG function: function code R; Predicted hydrolase of the alpha/beta superfamily

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the esterase D family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BESA_BACSU (O32102)

Other databases:

- EMBL:   AL009126
- RefSeq:   NP_391081.2
- ProteinModelPortal:   O32102
- SMR:   O32102
- EnsemblBacteria:   EBBACT00000002769
- GeneID:   936592
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU32010
- GenoList:   BSU32010
- GeneTree:   EBGT00050000002157
- HOGENOM:   HBG677453
- ProtClustDB:   CLSK887819
- BioCyc:   BSUB:BSU32010-MONOMER
- GO:   GO:0005737
- InterPro:   IPR000801

Pfam domain/function: PF00756 Esterase

EC number: NA

Molecular weight: Translated: 32439; Mature: 32439

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: ACT_SITE 163-163 ACT_SITE 225-225 ACT_SITE 263-263

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEQTTDRTNGGTSNAFTIPGTEVRMMSSRNENRTYHIFISKPSTPPPPAGYPVIYLLDA
CCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCCEEEEEEC
NSVFGTMTEAVRIQGRRPEKTGVIPAVIVGIGYETAEPFSSARHRDFTMPTAQSKLPERP
CCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHCCCCCCCCCHHHHCCCCC
DGREWPEHGGAEGFFRFIEEDLKPEIERDYQIDKKRQTIFGHSLGGLFVLQVLLTKPDAF
CCCCCCCCCCHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCHH
QTYIAGSPSIHWNKPFILKKTDHFVSLTKKNNQPINILLAAGELEQHHKSRMNDNARELY
HHEECCCCCCCCCCCEEEEECCCEEEEECCCCCCEEEEEECCHHHHHHHHHCCCCHHHHH
ERLAVLSEQGIRAEFCEFSGEGHISVLPVLVSRALRFALHPDGPHLSMG
HHHHHHHCCCCHHHHHHCCCCCCEEHHHHHHHHHHHEEECCCCCCCCCC
>Mature Secondary Structure
MKEQTTDRTNGGTSNAFTIPGTEVRMMSSRNENRTYHIFISKPSTPPPPAGYPVIYLLDA
CCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCCEEEEEEC
NSVFGTMTEAVRIQGRRPEKTGVIPAVIVGIGYETAEPFSSARHRDFTMPTAQSKLPERP
CCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHCCCCCCCCCHHHHCCCCC
DGREWPEHGGAEGFFRFIEEDLKPEIERDYQIDKKRQTIFGHSLGGLFVLQVLLTKPDAF
CCCCCCCCCCHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCHH
QTYIAGSPSIHWNKPFILKKTDHFVSLTKKNNQPINILLAAGELEQHHKSRMNDNARELY
HHEECCCCCCCCCCCEEEEECCCEEEEECCCCCCEEEEEECCHHHHHHHHHCCCCHHHHH
ERLAVLSEQGIRAEFCEFSGEGHISVLPVLVSRALRFALHPDGPHLSMG
HHHHHHHCCCCHHHHHHCCCCCCEEHHHHHHHHHHHEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377