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The map label for this gene is ytzG

Identifier: 255767687

GI number: 255767687

Start: 3072743

End: 3073462

Strand: Reverse

Name: ytzG

Synonym: BSU30035

Alternate gene names: 255767687

Gene position: 3073462-3072743 (Counterclockwise)

Preceding gene: 16080057

Following gene: 16080053

Centisome position: 72.91

GC content: 48.61

Gene sequence:

>720_bases
ATGAGACTTGATAAGCTGCTTGCCAACAGCGGGTATGGTTCCAGAAAAGAAGTCAAAGCCGTTGTGAAGGCTGGAGCCGT
GATGATCGACGGCAAACCTGCCAAAGATGTAAAGGAGCACGTTGATCCTGATACGCAGGAGGTGACCGTATACGGGGAAC
CGGTTGATTATCGAGAGTTCATTTATCTTATGATGAATAAACCGCAGGGCGTGCTGTCGGCGACTGAGGACAGCCGGCAG
CAAACAGTGGTCGATCTGCTGACGCCGGAGGAGATGAGATTTGAGCCATTTCCAGCCGGCAGGCTTGATAAAGATACAGA
GGGCTTTTTGCTGCTCACAAATGACGGCCAGCTCGCACATCGGCTCCTTTCTCCTAAAAAGCATGTTCCAAAAACGTATG
AAGTCCATTTAAAATCACAGATTTCTCGGGAAGACATTTCCGATTTGGAAACGGGTGTCTACATAGAGGGCGGCTATAAA
ACAAAGCCGGCAAAAGCGGAAATCAAAACAAACGACAGTGGCAATACCGTTATATATCTGACAATTACAGAAGGAAAATA
CCATCAGGTCAAGCAAATGGCAAAGGCAGTCGGCAATGAAGTGGTTTATCTGAAGCGGCTTTCGATGGGACGCGTTTCCC
TAGATCCCGCTCTGGCACCCGGAGAATACCGTGAGCTGACAGAGGAAGAGCTTCATTTGCTAAATGAGCCGCAAGCATGA

Upstream 100 bases:

>100_bases
GTTTCTTTAGGAAAGGCAGACATGCAGGCTGATGAACTAGATATGATGAAACTGAGGGCTCGCATGCTGCGGGCTTTCTT
TTATAAAGGAGAGATTGGGA

Downstream 100 bases:

>100_bases
CAAAAACCCCGCATACCGAATGCGGGGTTTTATTTATAAGCACACAAAAACAAGGCTCAGTAGTTTCTTCGCCCTGTTAT
CTATCGGACCGCTCTCAGGC

Product: 16S pseudouridylate synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MRLDKLLANSGYGSRKEVKAVVKAGAVMIDGKPAKDVKEHVDPDTQEVTVYGEPVDYREFIYLMMNKPQGVLSATEDSRQ
QTVVDLLTPEEMRFEPFPAGRLDKDTEGFLLLTNDGQLAHRLLSPKKHVPKTYEVHLKSQISREDISDLETGVYIEGGYK
TKPAKAEIKTNDSGNTVIYLTITEGKYHQVKQMAKAVGNEVVYLKRLSMGRVSLDPALAPGEYRELTEEELHLLNEPQA

Sequences:

>Translated_239_residues
MRLDKLLANSGYGSRKEVKAVVKAGAVMIDGKPAKDVKEHVDPDTQEVTVYGEPVDYREFIYLMMNKPQGVLSATEDSRQ
QTVVDLLTPEEMRFEPFPAGRLDKDTEGFLLLTNDGQLAHRLLSPKKHVPKTYEVHLKSQISREDISDLETGVYIEGGYK
TKPAKAEIKTNDSGNTVIYLTITEGKYHQVKQMAKAVGNEVVYLKRLSMGRVSLDPALAPGEYRELTEEELHLLNEPQA
>Mature_239_residues
MRLDKLLANSGYGSRKEVKAVVKAGAVMIDGKPAKDVKEHVDPDTQEVTVYGEPVDYREFIYLMMNKPQGVLSATEDSRQ
QTVVDLLTPEEMRFEPFPAGRLDKDTEGFLLLTNDGQLAHRLLSPKKHVPKTYEVHLKSQISREDISDLETGVYIEGGYK
TKPAKAEIKTNDSGNTVIYLTITEGKYHQVKQMAKAVGNEVVYLKRLSMGRVSLDPALAPGEYRELTEEELHLLNEPQA

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Escherichia coli, GI1788510, Length=233, Percent_Identity=40.7725321888412, Blast_Score=153, Evalue=8e-39,
Organism=Escherichia coli, GI1787524, Length=245, Percent_Identity=33.0612244897959, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1790453, Length=234, Percent_Identity=32.4786324786325, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI87081838, Length=182, Percent_Identity=32.4175824175824, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YTZG_BACSU (O32068)

Other databases:

- EMBL:   AL009126
- PIR:   H70004
- RefSeq:   NP_390882.2
- ProteinModelPortal:   O32068
- SMR:   O32068
- EnsemblBacteria:   EBBACT00000003436
- GeneID:   936165
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU30035
- NMPDR:   fig|224308.1.peg.3006
- GenoList:   BSU30035
- GeneTree:   EBGT00050000001923
- HOGENOM:   HBG754478
- ProtClustDB:   CLSK929071
- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942
- SMART:   SM00363
- TIGRFAMs:   TIGR00093

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26790; Mature: 26790

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS01149 PSI_RSU; PS50889 S4

Important sites: ACT_SITE 103-103

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKLLANSGYGSRKEVKAVVKAGAVMIDGKPAKDVKEHVDPDTQEVTVYGEPVDYREF
CCHHHHHHCCCCCCHHHHHHHHHCCEEEECCCCHHHHHHHCCCCCCEEEEEECCCCHHHE
IYLMMNKPQGVLSATEDSRQQTVVDLLTPEEMRFEPFPAGRLDKDTEGFLLLTNDGQLAH
EEEEECCCCCCEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHH
RLLSPKKHVPKTYEVHLKSQISREDISDLETGVYIEGGYKTKPAKAEIKTNDSGNTVIYL
HHHCCHHHCCCEEEEHHHHHCCHHHHHHHHCCEEEECCCCCCCCCEEEEECCCCCEEEEE
TITEGKYHQVKQMAKAVGNEVVYLKRLSMGRVSLDPALAPGEYRELTEEELHLLNEPQA
EECCCCHHHHHHHHHHHCCCEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRLDKLLANSGYGSRKEVKAVVKAGAVMIDGKPAKDVKEHVDPDTQEVTVYGEPVDYREF
CCHHHHHHCCCCCCHHHHHHHHHCCEEEECCCCHHHHHHHCCCCCCEEEEEECCCCHHHE
IYLMMNKPQGVLSATEDSRQQTVVDLLTPEEMRFEPFPAGRLDKDTEGFLLLTNDGQLAH
EEEEECCCCCCEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHH
RLLSPKKHVPKTYEVHLKSQISREDISDLETGVYIEGGYKTKPAKAEIKTNDSGNTVIYL
HHHCCHHHCCCEEEEHHHHHCCHHHHHHHHCCEEEECCCCCCCCCEEEEECCCCCEEEEE
TITEGKYHQVKQMAKAVGNEVVYLKRLSMGRVSLDPALAPGEYRELTEEELHLLNEPQA
EECCCCHHHHHHHHHHHCCCEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377