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The map label for this gene is relA

Identifier: 255767631

GI number: 255767631

Start: 2820529

End: 2822733

Strand: Reverse

Name: relA

Synonym: BSU27600

Alternate gene names: 255767631

Gene position: 2822733-2820529 (Counterclockwise)

Preceding gene: 16079815

Following gene: 50812276

Centisome position: 66.96

GC content: 44.31

Gene sequence:

>2205_bases
ATGGCGAACGAACAAGTATTGACTGCCGAGCAAGTTATAGATAAAGCACGCAGCTATCTATCTGATGAGCATATCGCATT
TGTCGAAAAAGCATATCTGTACGCTGAAGATGCTCATCGCGAGCAATACCGCAAATCGGGCGAGCCATATATTATTCATC
CGATTCAGGTTGCGGGGATACTCGTTGATCTTGAAATGGACCCTTCCACAATCGCGGGCGGATTTTTGCACGATGTCGTG
GAAGATACAGATGTGACGCTCGATGACCTGAAAGAAGCATTTTCCGAAGAAGTGGCAATGCTTGTAGACGGCGTAACGAA
ACTCGGCAAAATTAAATATAAATCTCAAGAGGAACAGCAGGCGGAAAATCATCGCAAAATGTTTGTCGCTATGGCTCAAG
ATATCAGGGTCATATTGATCAAGCTGGCGGATCGTCTTCACAATATGCGGACACTGAAACATCTGCCTCAGGAAAAACAG
CGGAGAATCTCCAATGAAACGCTGGAAATTTTTGCTCCTTTGGCGCATCGTCTCGGGATTTCAAAAATTAAGTGGGAATT
GGAAGATACGGCGCTCCGTTATTTGAACCCTCAGCAATATTACAGAATTGTCAACCTCATGAAGAAGAAACGTGCAGAAC
GAGAGCTTTATGTCGATGAGGTTGTCAATGAAGTGAAGAAACGTGTCGAAGAAGTAAATATCAAGGCTGACTTCTCGGGA
CGCCCGAAACATATTTACAGCATTTATCGAAAAATGGTGCTGCAAAATAAGCAATTCAATGAAATTTACGATTTGTTGGC
TGTCCGTATTCTTGTGAATAGCATAAAGGACTGCTACGCGGTGCTTGGCATCATTCACACATGCTGGAAACCGATGCCAG
GCAGATTCAAAGATTATATCGCAATGCCGAAGCCGAATATGTATCAATCGCTTCATACAACGGTTATTGGGCCTAAAGGC
GATCCGCTTGAAGTGCAGATCCGCACCTTTGAAATGCATGAAATAGCGGAATACGGGGTTGCGGCTCACTGGGCTTATAA
AGAAGGGAAAGCAGCCAATGAAGGTGCAACCTTTGAGAAAAAGCTTTCTTGGTTCCGTGAAATTTTAGAATTTCAAAATG
AATCGACAGATGCAGAAGAATTTATGGAATCGCTCAAAATTGATTTGTTCTCTGACATGGTGTATGTCTTTACGCCAAAA
GGAGATGTAATCGAGCTTCCGTCCGGTTCTGTTCCGATTGACTTTTCTTACCGGATTCACTCTGAAATCGGCAATAAAAC
AATCGGTGCCAAAGTAAACGGAAAAATGGTTACGCTTGACCATAAGCTTCGGACAGGTGATATCGTTGAAATTCTCACCT
CTAAGCATTCCTACGGTCCGAGCCAGGATTGGGTGAAGCTTGCCCAAACATCCCAAGCGAAGCATAAAATCCGTCAATTC
TTTAAGAAACAGCGGCGTGAAGAAAATGTCGAAAAAGGCCGTGAGCTGGTCGAAAAAGAAATTAAAAACTTGGATTTTGA
ATTGAAGGATGTTTTAACGCCGGAGAATATTCAAAAGGTTGCTGACAAATTTAATTTCTCAAATGAAGAGGATATGTACG
CGGCGGTCGGTTACAACGGCATCACAGCTCTGCAGGTGGCGAACCGCCTAACAGAAAAAGAGAGAAAGCAGCGCGACCAG
GAAGAACAGGAAAAGATCGTTCAGGAAGTCACTGGGGAACCTAAGCCATACCCGCAAGGAAGAAAACGGGAAGCTGGCGT
TCGTGTCAAGGGCATTGACAACCTCCTTGTCCGTTTATCAAAATGCTGCAATCCTGTGCCAGGTGATGATATTGTCGGCT
TTATCACAAAAGGCAGAGGGGTTTCGGTCCATCGCGAAGACTGTCCGAATGTCAAAACGAATGAAGCCCAAGAGCGGCTG
ATCCCGGTAGAGTGGGAACATGAGTCACAAGTTCAAAAGCGCAAGGAATACAATGTTGAGATAGAGATTCTTGGGTATGA
CCGCCGCGGATTGCTGAACGAGGTACTCCAGGCAGTGAATGAAACGAAAACCAATATTTCATCTGTCTCTGGCAAATCGG
ATCGCAATAAAGTGGCAACCATCCATATGGCGATTTTTATCCAGAATATCAATCACTTGCATAAAGTCGTCGAGCGTATT
AAACAGATTAGAGATATCTATTCTGTGCGCCGCGTCATGAACTAA

Upstream 100 bases:

>100_bases
TATATAATATTTGGCTATTTGAACTTCTGCTCTTTACATCTTTCGTTTTTTTCTTGATAATAAACTACAATACAGTTTAC
ATTTTAAAAAAGGTGATTCC

Downstream 100 bases:

>100_bases
AGGGGTTAGAAAAGAGATTAGTTGTTCAGCGAGTAACAGAAGCAAGCGTTACAGTGGACGAAGAGGTCGTCGGCCAAATT
GGACAAGGCCTTATGGTGCT

Product: GTP pyrophosphokinase RelA/SpoT

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I

Number of amino acids: Translated: 734; Mature: 733

Protein sequence:

>734_residues
MANEQVLTAEQVIDKARSYLSDEHIAFVEKAYLYAEDAHREQYRKSGEPYIIHPIQVAGILVDLEMDPSTIAGGFLHDVV
EDTDVTLDDLKEAFSEEVAMLVDGVTKLGKIKYKSQEEQQAENHRKMFVAMAQDIRVILIKLADRLHNMRTLKHLPQEKQ
RRISNETLEIFAPLAHRLGISKIKWELEDTALRYLNPQQYYRIVNLMKKKRAERELYVDEVVNEVKKRVEEVNIKADFSG
RPKHIYSIYRKMVLQNKQFNEIYDLLAVRILVNSIKDCYAVLGIIHTCWKPMPGRFKDYIAMPKPNMYQSLHTTVIGPKG
DPLEVQIRTFEMHEIAEYGVAAHWAYKEGKAANEGATFEKKLSWFREILEFQNESTDAEEFMESLKIDLFSDMVYVFTPK
GDVIELPSGSVPIDFSYRIHSEIGNKTIGAKVNGKMVTLDHKLRTGDIVEILTSKHSYGPSQDWVKLAQTSQAKHKIRQF
FKKQRREENVEKGRELVEKEIKNLDFELKDVLTPENIQKVADKFNFSNEEDMYAAVGYNGITALQVANRLTEKERKQRDQ
EEQEKIVQEVTGEPKPYPQGRKREAGVRVKGIDNLLVRLSKCCNPVPGDDIVGFITKGRGVSVHREDCPNVKTNEAQERL
IPVEWEHESQVQKRKEYNVEIEILGYDRRGLLNEVLQAVNETKTNISSVSGKSDRNKVATIHMAIFIQNINHLHKVVERI
KQIRDIYSVRRVMN

Sequences:

>Translated_734_residues
MANEQVLTAEQVIDKARSYLSDEHIAFVEKAYLYAEDAHREQYRKSGEPYIIHPIQVAGILVDLEMDPSTIAGGFLHDVV
EDTDVTLDDLKEAFSEEVAMLVDGVTKLGKIKYKSQEEQQAENHRKMFVAMAQDIRVILIKLADRLHNMRTLKHLPQEKQ
RRISNETLEIFAPLAHRLGISKIKWELEDTALRYLNPQQYYRIVNLMKKKRAERELYVDEVVNEVKKRVEEVNIKADFSG
RPKHIYSIYRKMVLQNKQFNEIYDLLAVRILVNSIKDCYAVLGIIHTCWKPMPGRFKDYIAMPKPNMYQSLHTTVIGPKG
DPLEVQIRTFEMHEIAEYGVAAHWAYKEGKAANEGATFEKKLSWFREILEFQNESTDAEEFMESLKIDLFSDMVYVFTPK
GDVIELPSGSVPIDFSYRIHSEIGNKTIGAKVNGKMVTLDHKLRTGDIVEILTSKHSYGPSQDWVKLAQTSQAKHKIRQF
FKKQRREENVEKGRELVEKEIKNLDFELKDVLTPENIQKVADKFNFSNEEDMYAAVGYNGITALQVANRLTEKERKQRDQ
EEQEKIVQEVTGEPKPYPQGRKREAGVRVKGIDNLLVRLSKCCNPVPGDDIVGFITKGRGVSVHREDCPNVKTNEAQERL
IPVEWEHESQVQKRKEYNVEIEILGYDRRGLLNEVLQAVNETKTNISSVSGKSDRNKVATIHMAIFIQNINHLHKVVERI
KQIRDIYSVRRVMN
>Mature_733_residues
ANEQVLTAEQVIDKARSYLSDEHIAFVEKAYLYAEDAHREQYRKSGEPYIIHPIQVAGILVDLEMDPSTIAGGFLHDVVE
DTDVTLDDLKEAFSEEVAMLVDGVTKLGKIKYKSQEEQQAENHRKMFVAMAQDIRVILIKLADRLHNMRTLKHLPQEKQR
RISNETLEIFAPLAHRLGISKIKWELEDTALRYLNPQQYYRIVNLMKKKRAERELYVDEVVNEVKKRVEEVNIKADFSGR
PKHIYSIYRKMVLQNKQFNEIYDLLAVRILVNSIKDCYAVLGIIHTCWKPMPGRFKDYIAMPKPNMYQSLHTTVIGPKGD
PLEVQIRTFEMHEIAEYGVAAHWAYKEGKAANEGATFEKKLSWFREILEFQNESTDAEEFMESLKIDLFSDMVYVFTPKG
DVIELPSGSVPIDFSYRIHSEIGNKTIGAKVNGKMVTLDHKLRTGDIVEILTSKHSYGPSQDWVKLAQTSQAKHKIRQFF
KKQRREENVEKGRELVEKEIKNLDFELKDVLTPENIQKVADKFNFSNEEDMYAAVGYNGITALQVANRLTEKERKQRDQE
EQEKIVQEVTGEPKPYPQGRKREAGVRVKGIDNLLVRLSKCCNPVPGDDIVGFITKGRGVSVHREDCPNVKTNEAQERLI
PVEWEHESQVQKRKEYNVEIEILGYDRRGLLNEVLQAVNETKTNISSVSGKSDRNKVATIHMAIFIQNINHLHKVVERIK
QIRDIYSVRRVMN

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain

Homologues:

Organism=Homo sapiens, GI38348360, Length=126, Percent_Identity=38.8888888888889, Blast_Score=67, Evalue=8e-11,
Organism=Escherichia coli, GI1790082, Length=719, Percent_Identity=40.0556328233658, Blast_Score=575, Evalue=1e-165,
Organism=Escherichia coli, GI1789147, Length=694, Percent_Identity=38.7608069164265, Blast_Score=530, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI25143118, Length=129, Percent_Identity=37.2093023255814, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24650996, Length=124, Percent_Identity=37.0967741935484, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RELA_BACSU (O54408)

Other databases:

- EMBL:   U86377
- EMBL:   AL009126
- PIR:   C69691
- RefSeq:   NP_390638.2
- ProteinModelPortal:   O54408
- EnsemblBacteria:   EBBACT00000002920
- GeneID:   936753
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU27600
- NMPDR:   fig|224308.1.peg.2763
- GenoList:   BSU27600
- GeneTree:   EBGT00050000000638
- HOGENOM:   HBG594146
- PhylomeDB:   O54408
- ProtClustDB:   CLSK887674
- BioCyc:   BSUB:BSU27600-MONOMER
- BRENDA:   2.7.6.5
- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676
- Gene3D:   G3DSA:3.10.20.30
- SMART:   SM00471
- SMART:   SM00954
- TIGRFAMs:   TIGR00691

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS; SSF81271 TGS-like

EC number: =2.7.6.5

Molecular weight: Translated: 84825; Mature: 84694

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANEQVLTAEQVIDKARSYLSDEHIAFVEKAYLYAEDAHREQYRKSGEPYIIHPIQVAGI
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEE
LVDLEMDPSTIAGGFLHDVVEDTDVTLDDLKEAFSEEVAMLVDGVTKLGKIKYKSQEEQQ
EEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
AENHRKMFVAMAQDIRVILIKLADRLHNMRTLKHLPQEKQRRISNETLEIFAPLAHRLGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHCCH
SKIKWELEDTALRYLNPQQYYRIVNLMKKKRAERELYVDEVVNEVKKRVEEVNIKADFSG
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCC
RPKHIYSIYRKMVLQNKQFNEIYDLLAVRILVNSIKDCYAVLGIIHTCWKPMPGRFKDYI
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHC
AMPKPNMYQSLHTTVIGPKGDPLEVQIRTFEMHEIAEYGVAAHWAYKEGKAANEGATFEK
CCCCCHHHHHHHHEEECCCCCCEEEEEEEEHHHHHHHHCHHHEEECCCCCCCCCCCCHHH
KLSWFREILEFQNESTDAEEFMESLKIDLFSDMVYVFTPKGDVIELPSGSVPIDFSYRIH
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCEEECCCCCCCEEEEEEEH
SEIGNKTIGAKVNGKMVTLDHKLRTGDIVEILTSKHSYGPSQDWVKLAQTSQAKHKIRQF
HHCCCCEECCEECCEEEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
FKKQRREENVEKGRELVEKEIKNLDFELKDVLTPENIQKVADKFNFSNEEDMYAAVGYNG
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHCCCCCCCCCEEEECCCC
ITALQVANRLTEKERKQRDQEEQEKIVQEVTGEPKPYPQGRKREAGVRVKGIDNLLVRLS
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECHHHHHHHHH
KCCNPVPGDDIVGFITKGRGVSVHREDCPNVKTNEAQERLIPVEWEHESQVQKRKEYNVE
HHCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCEE
IEILGYDRRGLLNEVLQAVNETKTNISSVSGKSDRNKVATIHMAIFIQNINHLHKVVERI
EEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KQIRDIYSVRRVMN
HHHHHHHHHHHHCC
>Mature Secondary Structure 
ANEQVLTAEQVIDKARSYLSDEHIAFVEKAYLYAEDAHREQYRKSGEPYIIHPIQVAGI
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEE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HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9383190; 9384377