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The map label for this gene is arsB

Identifier: 255767593

GI number: 255767593

Start: 2655753

End: 2656793

Strand: Reverse

Name: arsB

Synonym: BSU25790

Alternate gene names: 255767593

Gene position: 2656793-2655753 (Counterclockwise)

Preceding gene: 255767594

Following gene: 16079631

Centisome position: 63.02

GC content: 36.12

Gene sequence:

>1041_bases
ATGAAACGTTTATCTTTTTTAGACCGTTATTTAACCATCTGGATTTTTCTTGCGATGGCATTGGGGATAGGATTAGGTTT
TATATTTCCGAGTTTTGTTGGTGGATTAAACAAATTACAAGTAGGAACTACATCTATCCCTTTAGCTATTGGATTAGTAT
TAATGATGTATCCACCACTAGCAAAGGTTCGATATGAAGAAATTGGACGGGTATTTAAAGATATTAAAGTGTTAATTTTA
TCTTTAGTGCAAAACTGGATCATCGGACCGACTCTCATGTTTATTTTGGCTATCATCTTTTTGCCAGATAAACCGGAGTA
TATGATTGGGCTTATTATGATTGGATTAGCTCGCTGTATTGCGATGGTTATTGTTTGGAATGACTTATCAAAAGGTGATA
CGGAATACGCGGCAGGATTAGTTGCTTTCAACTCAATTTTCCAAATGCTTTTTTTCTCAGTTTATGCTTACATTTTTGTA
ACAGTTATTCCGCAATGGTTAGGGATGGAAGGAGCAGTAGTGAATATTACGATGGCAGAAGTCGCAAAATCAGTATTCAT
TTACCTTGGAGTTCCTTTTATTGCAGGAATGGTGACACGTTATATTTTTGTAAAAGTGAAGGGCAAAGAATGGTATGAAA
AAGTGTTCATTCCTAAGATTAGTCCAATTACGTTAATTGCTTTGCTATTTACAATTATCGTCATGTTTTCCTTAAAAGGT
GATGTAATTGTTAGCTTGCCGTTAGATGTTGTCCGAGTTGCGATTCCATTATTGATTTACTTTGTTTTAATGTTCTTTGT
TTCCTTCTTTTTAGGGAAGAAAATCGGGGCAAACTATGCTGTAACTACTACTTTAGCTTTTACAGCAGGAAGCAATAACT
TTGAATTAGCGATTGCTGTAGCTGTTGGTGTATTTGGAATTCATTCGGGTGCAGCATTTGCCGCCGTAATTGGTCCGCTT
GTAGAAGTGCCAGTCATGATAGCACTTGTTAAGGTTGCTTTATGGTTTCAACGAAAATACTTTGGAAGTCACTCGATGTA
G

Upstream 100 bases:

>100_bases
AGCGAGGTTCAAAAACAAGATTCCTCCTCTTGTTGTGTAACACCGCCAAGTGATATTACAACGAACTCTTGCTGTTAGTA
AAAAAGGTGAGGATAAAGTA

Downstream 100 bases:

>100_bases
AGGTGGAATATATGGAGAATAAAATCATTTACTTTTTATGTACAGGGAACTCTTGCCGGAGCCAAATGGCTGAAGGATGG
GCTAAACAATATTTAGGTGA

Product: arsenite efflux transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MKRLSFLDRYLTIWIFLAMALGIGLGFIFPSFVGGLNKLQVGTTSIPLAIGLVLMMYPPLAKVRYEEIGRVFKDIKVLIL
SLVQNWIIGPTLMFILAIIFLPDKPEYMIGLIMIGLARCIAMVIVWNDLSKGDTEYAAGLVAFNSIFQMLFFSVYAYIFV
TVIPQWLGMEGAVVNITMAEVAKSVFIYLGVPFIAGMVTRYIFVKVKGKEWYEKVFIPKISPITLIALLFTIIVMFSLKG
DVIVSLPLDVVRVAIPLLIYFVLMFFVSFFLGKKIGANYAVTTTLAFTAGSNNFELAIAVAVGVFGIHSGAAFAAVIGPL
VEVPVMIALVKVALWFQRKYFGSHSM

Sequences:

>Translated_346_residues
MKRLSFLDRYLTIWIFLAMALGIGLGFIFPSFVGGLNKLQVGTTSIPLAIGLVLMMYPPLAKVRYEEIGRVFKDIKVLIL
SLVQNWIIGPTLMFILAIIFLPDKPEYMIGLIMIGLARCIAMVIVWNDLSKGDTEYAAGLVAFNSIFQMLFFSVYAYIFV
TVIPQWLGMEGAVVNITMAEVAKSVFIYLGVPFIAGMVTRYIFVKVKGKEWYEKVFIPKISPITLIALLFTIIVMFSLKG
DVIVSLPLDVVRVAIPLLIYFVLMFFVSFFLGKKIGANYAVTTTLAFTAGSNNFELAIAVAVGVFGIHSGAAFAAVIGPL
VEVPVMIALVKVALWFQRKYFGSHSM
>Mature_346_residues
MKRLSFLDRYLTIWIFLAMALGIGLGFIFPSFVGGLNKLQVGTTSIPLAIGLVLMMYPPLAKVRYEEIGRVFKDIKVLIL
SLVQNWIIGPTLMFILAIIFLPDKPEYMIGLIMIGLARCIAMVIVWNDLSKGDTEYAAGLVAFNSIFQMLFFSVYAYIFV
TVIPQWLGMEGAVVNITMAEVAKSVFIYLGVPFIAGMVTRYIFVKVKGKEWYEKVFIPKISPITLIALLFTIIVMFSLKG
DVIVSLPLDVVRVAIPLLIYFVLMFFVSFFLGKKIGANYAVTTTLAFTAGSNNFELAIAVAVGVFGIHSGAAFAAVIGPL
VEVPVMIALVKVALWFQRKYFGSHSM

Specific function: Seems to confer resistance to arsenite by allowing cells to extrude this compound. Could be part of an arsenite extrusion pump

COG id: COG0798

COG function: function code P; Arsenite efflux pump ACR3 and related permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ACR3 family

Homologues:

Organism=Saccharomyces cerevisiae, GI6325459, Length=320, Percent_Identity=35.9375, Blast_Score=176, Evalue=5e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ARSB_BACSU (P45946)

Other databases:

- EMBL:   D32216
- EMBL:   D84432
- EMBL:   AL009126
- PIR:   B69950
- RefSeq:   NP_390456.2
- EnsemblBacteria:   EBBACT00000003706
- GeneID:   937800
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU25790
- NMPDR:   fig|224308.1.peg.2581
- GenoList:   BSU25790
- GeneTree:   EBGT00070000032046
- HOGENOM:   HBG519064
- PhylomeDB:   P45946
- ProtClustDB:   CLSK887612
- BioCyc:   BSUB:BSU25790-MONOMER
- InterPro:   IPR004706
- InterPro:   IPR002657
- PANTHER:   PTHR10361
- PIRSF:   PIRSF005508
- TIGRFAMs:   TIGR00832

Pfam domain/function: PF01758 SBF

EC number: NA

Molecular weight: Translated: 38312; Mature: 38312

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x10e241d0)-; HASH(0x109d24b0)-; HASH(0x10d7f344)-; HASH(0x1111ecd4)-; HASH(0xfb9cbec)-; HASH(0xf99fba4)-; HASH(0x11095ab0)-; HASH(0x102d1060)-; HASH(0xfda3c2c)-; HASH(0xf378188)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLSFLDRYLTIWIFLAMALGIGLGFIFPSFVGGLNKLQVGTTSIPLAIGLVLMMYPPL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCCHHHHHHHHHHHHCCCH
AKVRYEEIGRVFKDIKVLILSLVQNWIIGPTLMFILAIIFLPDKPEYMIGLIMIGLARCI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AMVIVWNDLSKGDTEYAAGLVAFNSIFQMLFFSVYAYIFVTVIPQWLGMEGAVVNITMAE
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHH
VAKSVFIYLGVPFIAGMVTRYIFVKVKGKEWYEKVFIPKISPITLIALLFTIIVMFSLKG
HHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
DVIVSLPLDVVRVAIPLLIYFVLMFFVSFFLGKKIGANYAVTTTLAFTAGSNNFELAIAV
CEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCEEHHHH
AVGVFGIHSGAAFAAVIGPLVEVPVMIALVKVALWFQRKYFGSHSM
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
>Mature Secondary Structure
MKRLSFLDRYLTIWIFLAMALGIGLGFIFPSFVGGLNKLQVGTTSIPLAIGLVLMMYPPL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCCHHHHHHHHHHHHCCCH
AKVRYEEIGRVFKDIKVLILSLVQNWIIGPTLMFILAIIFLPDKPEYMIGLIMIGLARCI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AMVIVWNDLSKGDTEYAAGLVAFNSIFQMLFFSVYAYIFVTVIPQWLGMEGAVVNITMAE
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHH
VAKSVFIYLGVPFIAGMVTRYIFVKVKGKEWYEKVFIPKISPITLIALLFTIIVMFSLKG
HHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
DVIVSLPLDVVRVAIPLLIYFVLMFFVSFFLGKKIGANYAVTTTLAFTAGSNNFELAIAV
CEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCEEHHHH
AVGVFGIHSGAAFAAVIGPLVEVPVMIALVKVALWFQRKYFGSHSM
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7704261; 8969508; 9384377; 7489895; 9537360