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The map label for this gene is recO

Identifier: 255767577

GI number: 255767577

Start: 2608946

End: 2609713

Strand: Reverse

Name: recO

Synonym: BSU25280

Alternate gene names: 255767577

Gene position: 2609713-2608946 (Counterclockwise)

Preceding gene: 255767578

Following gene: 16079581

Centisome position: 61.91

GC content: 39.45

Gene sequence:

>768_bases
ATGCTGACAAAATGTGAAGGGATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACATTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATGTTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGCC
AAATCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGTTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTGAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGGCTCTTTTACTATTTTGATCTGTCGAGACTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Upstream 100 bases:

>100_bases
GAACATGGAAAGACCCGAAGAACTAGAAGATGAGCTAGCCCGATTTGATTATCCAATTTTATAAACCTCTTGGCCGCTCC
TGCTTGAGGAAGGTGCGGAA

Downstream 100 bases:

>100_bases
CATTTGGTCCATCTTTTTATATGATCATTTATTATAAAATATGTTGCAGTGAGAGAAAGAAGTACTTGCGTTTACCTCAT
GAAAGCGACCTTAGGGCGGT

Product: DNA repair protein RecO

Products: NA

Alternate protein names: Recombination protein O

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYVAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Sequences:

>Translated_255_residues
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYVAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS
>Mature_255_residues
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYVAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Specific function: Plays a role in DNA double-stranded break repair. It seems to be involved in an recBC-independent recombinational process of DNA repair. It may act with recF and recR. Is recruited to repair centers, foci that are the site of double-strand break(s) after

COG id: COG1381

COG function: function code L; Recombinational DNA repair protein (RecF pathway)

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recO family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECO_BACSU (P42095)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- EMBL:   U18532
- PIR:   H69968
- RefSeq:   NP_390406.2
- ProteinModelPortal:   P42095
- EnsemblBacteria:   EBBACT00000000653
- GeneID:   937881
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU25280
- NMPDR:   fig|224308.1.peg.2531
- GenoList:   BSU25280
- GeneTree:   EBGT00050000001475
- HOGENOM:   HBG634481
- ProtClustDB:   PRK00085
- BioCyc:   BSUB:BSU25280-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00201
- InterPro:   IPR001164
- InterPro:   IPR022572
- InterPro:   IPR016027
- InterPro:   IPR003717
- TIGRFAMs:   TIGR00613

Pfam domain/function: PF02565 RecO; PF11967 RecO_N; SSF57863 ArfGAP; SSF50249 Nucleic_acid_OB

EC number: NA

Molecular weight: Translated: 29328; Mature: 29328

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLM
CCCCCCEEEEEECCCCCCCCEEEEEECCCCCEEEEECCCCCCCHHHHHHHCCHHHHHHHH
QKTSGLGTLQQGEMILSMRGIREDLFLTAYAAYVAELVDRGTEEKKPNPYLFEFILESLK
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
QLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDGTFHFSVRDNGFICHRCFEKD
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHEEEECCCCCEEEEEECCCCEEEEECCCCC
PYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
CCEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEHHHHHH
DQMESMKHLMGENKS
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLM
CCCCCCEEEEEECCCCCCCCEEEEEECCCCCEEEEECCCCCCCHHHHHHHCCHHHHHHHH
QKTSGLGTLQQGEMILSMRGIREDLFLTAYAAYVAELVDRGTEEKKPNPYLFEFILESLK
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
QLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDGTFHFSVRDNGFICHRCFEKD
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHEEEECCCCCEEEEEECCCCEEEEECCCCC
PYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
CCEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEHHHHHH
DQMESMKHLMGENKS
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10323239