Click here to switch to the map view.

The map label for this gene is ispH

Identifier: 255767572

GI number: 255767572

Start: 2596535

End: 2597479

Strand: Direct

Name: ispH

Synonym: BSU25160

Alternate gene names: 255767572

Gene position: 2596535-2597479 (Clockwise)

Preceding gene: 16079570

Following gene: 50812269

Centisome position: 61.59

GC content: 42.86

Gene sequence:

>945_bases
ATGGACGTAATTAAAATTTCACCGCGCGGCTATTGCTATGGTGTTGTTGATGCTATGGTCATTGCCAAAAATGCGTCGCT
AGATAAAAGCTTGCCAAGACCCATCTATATACTCGGGATGATTGTTCATAATAAACACGTCACTGATGCGTTTGAAGAGG
AAGGGATTTTTACATTGGACGGCTCTAACCGTCTGGACATCTTAAAGCAGGTTGACAAAGGAACTGTCATTTTCACTGCG
CACGGTGTTTCTCCCGAAGTCAGAAGAATCGCAGAAGAAAAAGGGTTAGTCGCAATTGATGCTACATGTCCGGATGTGAC
AAAAACTCATAATCTAATCTTAGAGATGAAAGAAAAAGGATATCACGTGATCTATATCGGCAAAAAAGGGCATCCAGAGC
CTGAGGGAGCCGTTGGCGTAGCGCCGGAGATCGTTCACTTAGTTGAAACAGAAGAAGATGTAAAGAACCTTGATATCCAA
TCTGAGAAGTTAATCGTGACGAATCAAACAACAATGTCTCAATGGGATGTTCACGACATTATGGAGCTTGTCAAAGAAAA
ATATCCTCATGTCGAATATCACCAAGAGATTTGCCTCGCTACTCAAGTGCGGCAGGAAGCTGTATCAGAGCAGGCAAAAA
AAGCGGACTTAACAATCGTTGTCGGAGACCCGAAAAGCAACAATTCAAATAGGCTTGCTCAAGTGTCAGAGGAAATAGCC
GGGACAAAAGCATACCGGATCGGCGACTTAAGCGAATTGAAGCTCGAATGGCTGAAAGGCGTTAATACCGTTGCAGTTAC
AGCGGGAGCTTCAACACCGACGCCGATCACAAAAGAAGTCATTCGCTTTTTAGAGCAGTTTGATCACGAAGATCCATCAA
CTTGGACAACTGAACATAATATACCGCTCAAAAAAATACTTCCAAAAGTAAAAGCAAAAAACTGA

Upstream 100 bases:

>100_bases
GATTGGCTATAATGAGATCAGAAGAATGCAAGAATAGAGCCGTTTGATTACGGCTTTTTATATTGCCTTTACTCACTTCA
GTATTGGAGGACAACCGAAA

Downstream 100 bases:

>100_bases
GGCTGTAAAAGCCTCAGTTTTTTTATAGAAATGTAAATGGATTTGTATCTGTTTCAGAAACAAAGATATTCACGCCAAAC
TTTTTATCATTACACATTGA

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MDVIKISPRGYCYGVVDAMVIAKNASLDKSLPRPIYILGMIVHNKHVTDAFEEEGIFTLDGSNRLDILKQVDKGTVIFTA
HGVSPEVRRIAEEKGLVAIDATCPDVTKTHNLILEMKEKGYHVIYIGKKGHPEPEGAVGVAPEIVHLVETEEDVKNLDIQ
SEKLIVTNQTTMSQWDVHDIMELVKEKYPHVEYHQEICLATQVRQEAVSEQAKKADLTIVVGDPKSNNSNRLAQVSEEIA
GTKAYRIGDLSELKLEWLKGVNTVAVTAGASTPTPITKEVIRFLEQFDHEDPSTWTTEHNIPLKKILPKVKAKN

Sequences:

>Translated_314_residues
MDVIKISPRGYCYGVVDAMVIAKNASLDKSLPRPIYILGMIVHNKHVTDAFEEEGIFTLDGSNRLDILKQVDKGTVIFTA
HGVSPEVRRIAEEKGLVAIDATCPDVTKTHNLILEMKEKGYHVIYIGKKGHPEPEGAVGVAPEIVHLVETEEDVKNLDIQ
SEKLIVTNQTTMSQWDVHDIMELVKEKYPHVEYHQEICLATQVRQEAVSEQAKKADLTIVVGDPKSNNSNRLAQVSEEIA
GTKAYRIGDLSELKLEWLKGVNTVAVTAGASTPTPITKEVIRFLEQFDHEDPSTWTTEHNIPLKKILPKVKAKN
>Mature_314_residues
MDVIKISPRGYCYGVVDAMVIAKNASLDKSLPRPIYILGMIVHNKHVTDAFEEEGIFTLDGSNRLDILKQVDKGTVIFTA
HGVSPEVRRIAEEKGLVAIDATCPDVTKTHNLILEMKEKGYHVIYIGKKGHPEPEGAVGVAPEIVHLVETEEDVKNLDIQ
SEKLIVTNQTTMSQWDVHDIMELVKEKYPHVEYHQEICLATQVRQEAVSEQAKKADLTIVVGDPKSNNSNRLAQVSEEIA
GTKAYRIGDLSELKLEWLKGVNTVAVTAGASTPTPITKEVIRFLEQFDHEDPSTWTTEHNIPLKKILPKVKAKN

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=293, Percent_Identity=38.2252559726962, Blast_Score=188, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 34958; Mature: 34958

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVIKISPRGYCYGVVDAMVIAKNASLDKSLPRPIYILGMIVHNKHVTDAFEEEGIFTLD
CCEEEECCCCEEHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHCCEEEEC
GSNRLDILKQVDKGTVIFTAHGVSPEVRRIAEEKGLVAIDATCPDVTKTHNLILEMKEKG
CCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCEEEECCCCCCHHHHHEEEEEECCC
YHVIYIGKKGHPEPEGAVGVAPEIVHLVETEEDVKNLDIQSEKLIVTNQTTMSQWDVHDI
EEEEEECCCCCCCCCCCCCCCHHHHHHHCCHHHHHCCCCCCCEEEEECCCCCCCCCHHHH
MELVKEKYPHVEYHQEICLATQVRQEAVSEQAKKADLTIVVGDPKSNNSNRLAQVSEEIA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHH
GTKAYRIGDLSELKLEWLKGVNTVAVTAGASTPTPITKEVIRFLEQFDHEDPSTWTTEHN
CCCEEECCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
IPLKKILPKVKAKN
CCHHHHHHHHCCCC
>Mature Secondary Structure
MDVIKISPRGYCYGVVDAMVIAKNASLDKSLPRPIYILGMIVHNKHVTDAFEEEGIFTLD
CCEEEECCCCEEHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHCCEEEEC
GSNRLDILKQVDKGTVIFTAHGVSPEVRRIAEEKGLVAIDATCPDVTKTHNLILEMKEKG
CCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCEEEECCCCCCHHHHHEEEEEECCC
YHVIYIGKKGHPEPEGAVGVAPEIVHLVETEEDVKNLDIQSEKLIVTNQTTMSQWDVHDI
EEEEEECCCCCCCCCCCCCCCHHHHHHHCCHHHHHCCCCCCCEEEEECCCCCCCCCHHHH
MELVKEKYPHVEYHQEICLATQVRQEAVSEQAKKADLTIVVGDPKSNNSNRLAQVSEEIA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHH
GTKAYRIGDLSELKLEWLKGVNTVAVTAGASTPTPITKEVIRFLEQFDHEDPSTWTTEHN
CCCEEECCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
IPLKKILPKVKAKN
CCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA