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The map label for this gene is pstA

Identifier: 255767564

GI number: 255767564

Start: 2578833

End: 2579717

Strand: Reverse

Name: pstA

Synonym: BSU24970

Alternate gene names: 255767564

Gene position: 2579717-2578833 (Counterclockwise)

Preceding gene: 255767565

Following gene: 16079551

Centisome position: 61.19

GC content: 44.63

Gene sequence:

>885_bases
ATGAACCGCAAAATAACAGATAAACTGGCGACCGGAATGTTTGGTTTGTGTGCAGCCATTATCGCTGCCATTTTAGTAGG
TTTGTTTTCCTATATTATTATAAACGGTGTTTCTCAGCTCAGTTTTCAATTTATTACAACAAAGTCGAGCGCCATTGCAG
CTGGAGGAGGCATACGAGATCAGCTGTTTAACTCCTTTTATATTTTGTTTATTACAATGCTGATTACAATCCCGCTCGGT
GTGGGCGGCGGAGTCTTCATGGCTGAATACGCGCCTAATAACAAAGTAACTGACTTCATTAGAACATGTATAGAGGTGCT
TTCATCTCTTCCGTCTATTGTCATTGGGATGTTTGGATTATTGATGTTTGTCAACTTAACAGGCTGGGGTTATACGATCA
TAGGCGGAGCGCTTGCGTTAACTGTTTTTAACCTTCCTGTCATGGTGCGTGTGACAGAAGACGCGATCCGTTCAGTCCCT
AAAGATCTGAAGGAAGCCTCTCTCGCTTTAGGTGTATCACGCTGGCATACCGTAAAAACAGTTTTAATTCCAAGTGCGAT
TCCTTCTATCATCACAGGAGCAATTTTAGCGTCAGGAAGGGTGTTCGGGGAAGCGGCCGCATTATTATTTACGGCAGGCC
TGACAACACCGCGTCTTAATTTTACGGAGTGGAACCCGTTTTCGGAAACATCACCGCTTAATATTTTCAGGCCCGCTGAA
ACACTTGCGGTCCACATTTGGAACGTCAATACACAAGGAATGATTCCGGATGCTGAAGCGATTGCCAACGGTGGTTCTGC
CGTGCTGGTGATTTCTGTTCTTGTGTTTAATCTCGCGGCGAGATGGCTTGGCACGATGATCTACAAAAAGCTTACGGCAA
ATTAG

Upstream 100 bases:

>100_bases
TTGAAAACAATACGCTGTGGTCAATGGGGCTTGTGCTTCTGGTGATGTCATTCTTGTTCATCCTGCTTATTCGCTACTTG
TCATCTAGGAGGAAAGTGTA

Downstream 100 bases:

>100_bases
AACGGAAAGAGGCGAAAAACATGTCTGAACAAATGGTAAAAGAAAAACCTGAGCGAGCTGTTATTGTTCCGAAGCAGAAC
CACGTGCTTGAGGTCAAAGA

Product: phosphate ABC transporter permease

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MNRKITDKLATGMFGLCAAIIAAILVGLFSYIIINGVSQLSFQFITTKSSAIAAGGGIRDQLFNSFYILFITMLITIPLG
VGGGVFMAEYAPNNKVTDFIRTCIEVLSSLPSIVIGMFGLLMFVNLTGWGYTIIGGALALTVFNLPVMVRVTEDAIRSVP
KDLKEASLALGVSRWHTVKTVLIPSAIPSIITGAILASGRVFGEAAALLFTAGLTTPRLNFTEWNPFSETSPLNIFRPAE
TLAVHIWNVNTQGMIPDAEAIANGGSAVLVISVLVFNLAARWLGTMIYKKLTAN

Sequences:

>Translated_294_residues
MNRKITDKLATGMFGLCAAIIAAILVGLFSYIIINGVSQLSFQFITTKSSAIAAGGGIRDQLFNSFYILFITMLITIPLG
VGGGVFMAEYAPNNKVTDFIRTCIEVLSSLPSIVIGMFGLLMFVNLTGWGYTIIGGALALTVFNLPVMVRVTEDAIRSVP
KDLKEASLALGVSRWHTVKTVLIPSAIPSIITGAILASGRVFGEAAALLFTAGLTTPRLNFTEWNPFSETSPLNIFRPAE
TLAVHIWNVNTQGMIPDAEAIANGGSAVLVISVLVFNLAARWLGTMIYKKLTAN
>Mature_294_residues
MNRKITDKLATGMFGLCAAIIAAILVGLFSYIIINGVSQLSFQFITTKSSAIAAGGGIRDQLFNSFYILFITMLITIPLG
VGGGVFMAEYAPNNKVTDFIRTCIEVLSSLPSIVIGMFGLLMFVNLTGWGYTIIGGALALTVFNLPVMVRVTEDAIRSVP
KDLKEASLALGVSRWHTVKTVLIPSAIPSIITGAILASGRVFGEAAALLFTAGLTTPRLNFTEWNPFSETSPLNIFRPAE
TLAVHIWNVNTQGMIPDAEAIANGGSAVLVISVLVFNLAARWLGTMIYKKLTAN

Specific function: Part of the binding-protein-dependent transport system yqgGHIJK. Probably responsible for the translocation of the substrate across the membrane

COG id: COG0581

COG function: function code P; ABC-type phosphate transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain

Homologues:

Organism=Escherichia coli, GI1790163, Length=279, Percent_Identity=30.4659498207885, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1790164, Length=283, Percent_Identity=25.4416961130742, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YQGI_BACSU (P46340)

Other databases:

- EMBL:   D58414
- EMBL:   D84432
- EMBL:   AL009126
- PIR:   C69956
- RefSeq:   NP_390376.2
- ProteinModelPortal:   P46340
- SMR:   P46340
- EnsemblBacteria:   EBBACT00000003727
- GeneID:   938200
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU24970
- NMPDR:   fig|224308.1.peg.2501
- GenoList:   BSU24970
- GeneTree:   EBGT00050000000644
- HOGENOM:   HBG705496
- PhylomeDB:   P46340
- ProtClustDB:   CLSK887580
- BioCyc:   BSUB:BSU24970-MONOMER
- InterPro:   IPR000515
- InterPro:   IPR005672
- TIGRFAMs:   TIGR00974

Pfam domain/function: PF00528 BPD_transp_1

EC number: NA

Molecular weight: Translated: 31437; Mature: 31437

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x10c1853c)-; HASH(0x10e16f3c)-; HASH(0x10df0550)-; HASH(0xf81e2ac)-; HASH(0x10a63b74)-; HASH(0x10bde48c)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRKITDKLATGMFGLCAAIIAAILVGLFSYIIINGVSQLSFQFITTKSSAIAAGGGIRD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHCCCCCHHH
QLFNSFYILFITMLITIPLGVGGGVFMAEYAPNNKVTDFIRTCIEVLSSLPSIVIGMFGL
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LMFVNLTGWGYTIIGGALALTVFNLPVMVRVTEDAIRSVPKDLKEASLALGVSRWHTVKT
HHHHHCCCCCHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLIPSAIPSIITGAILASGRVFGEAAALLFTAGLTTPRLNFTEWNPFSETSPLNIFRPAE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCC
TLAVHIWNVNTQGMIPDAEAIANGGSAVLVISVLVFNLAARWLGTMIYKKLTAN
EEEEEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MNRKITDKLATGMFGLCAAIIAAILVGLFSYIIINGVSQLSFQFITTKSSAIAAGGGIRD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHCCCCCHHH
QLFNSFYILFITMLITIPLGVGGGVFMAEYAPNNKVTDFIRTCIEVLSSLPSIVIGMFGL
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LMFVNLTGWGYTIIGGALALTVFNLPVMVRVTEDAIRSVPKDLKEASLALGVSRWHTVKT
HHHHHCCCCCHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLIPSAIPSIITGAILASGRVFGEAAALLFTAGLTTPRLNFTEWNPFSETSPLNIFRPAE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCC
TLAVHIWNVNTQGMIPDAEAIANGGSAVLVISVLVFNLAARWLGTMIYKKLTAN
EEEEEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8760913; 8969508; 9384377