Definition Bacillus subtilis subsp. subtilis str. 168 chromosome, complete genome.
Accession NC_000964
Length 4,215,606

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The map label for this gene is glpG

Identifier: 255767560

GI number: 255767560

Start: 2571907

End: 2573430

Strand: Reverse

Name: glpG

Synonym: BSU24870

Alternate gene names: 255767560

Gene position: 2573430-2571907 (Counterclockwise)

Preceding gene: 16079544

Following gene: 16079542

Centisome position: 61.05

GC content: 44.95

Gene sequence:

>1524_bases
ATGTTTTTGCTGGAGTATACATATTGGAAAATTGCGGCGCACCTGGTGAATAGCGGGTATGGCGTCATACAAGCCGGTGA
GTCAGATGAAATATGGCTGGAAGCGCCGGACAAGTCCTCTCATGATCTAGTTCGTCTTTATAAGCACGATCTTGATTTCC
GTCAAGAAATGGTTAGAGACATCGAAGAGCAGGCCGAACGAGTAGAACGAGTCAGACATCAATTGGGCAGACGAAGGATG
AAGCTGCTGAACGTGTTTTTTTCAACGGAAGCCCCAGTTGATGACTGGGAAGAGATTGCAAAAAAAACTTTTGAGAAGGG
AACTGTGTCAGTTGAGCCGGCGATCGTTAGAGGAACGATGCTTCGAGATGATCTTCAAGCGGTTTTTCCATCATTTCGTA
CAGAAGATTGTTCAGAGGAACACGCTTCGTTTGAAAACGCGCAAATGGCGCGAGAACGCTTCCTTTCGCTAGTGCTGAAA
CAAGAAGAACAGAGAAAAACTGAAGCGGCGGTTTTTCAAAACGGAAAACCGACTTTTACATATTTATTTATTGCGCTTCA
GATCCTGATGTTTTTCTTGCTCGAAATAAACGGAGGCAGCACTAATACGGAGACACTGGTGGCTTTTGGAGCAAAAGAAA
ACAGCCTGATTGCACAAGGAGAATGGTGGCGGCTTCTGACACCGATCGTGCTTCACATCGGCATAGCGCATTTAGCTTTT
AATACGCTGGCTTTATGGTCGGTTGGTACAGCGGTTGAGCGGATGTATGGATCGGGGAGATTTTTGCTGATTTATCTGGC
TGCTGGCATTACGGGTTCGATTGCGAGTTTTGTGTTCAGCCCGTATCCTTCGGCAGGGGCATCAGGAGCCATATTCGGAT
GCTTAGGTGCATTACTGTATGTTGCTTTGTCTAATCGAAAAATGTTTTTAAGAACTATAGGAACCAATATTATTGTCATT
ATTATCATCAATCTGGGCTTTGGTTTTGCGGTTTCGAATATTGATAATTCAGGACATATCGGCGGCTTGATTGGCGGCTT
TTTCGCTGCAGCAGCACTGGGGTTGCCTAAAGCCGGAGCCTTTGGAAAAAGATTGTTGTCAGCGGTTTTGCTGATTGCTT
TGGCTGTTGGATTTTTATATTACGGATTGCATTCGCCTTCACACCAGGAGTCAGCGCTGATTCAGCAGGCAAGCGAGCTG
TATCAGGAAGGAAAGTATGAAGAAGTAACAGAATTGCTGAACGGAGAAGCAGCGCAAAAAGATGCCTCTGCCGATCTTTT
GAAAATTCTTGCTGTTTCTGATATTCAAATCGGCGAATATGATCAGGCGGTTTCCCTTTTGGAACGAGCAGTGAAAAAAG
AACCTAAAGACCATGCTTCCTATTACAATTTAGCGTTATTGTATGCGGAAAAAAATGAGCTTGCACAAGCAGAAAAGGCC
ATCCAGACGGCTGTGAAACTGAAGCCGAAGGAGCAGCGCTACAAGGAGCTGCAGCGGCAAATTGAAAACAATAAAGAATC
ATAG

Upstream 100 bases:

>100_bases
AAGCTTTCTAAAATCCCGTGAAAAACGGGATTTTTTGCTTTCAGTAGGCGTTTCGGAGCGCCAGCTGTTATAATGAAAAA
AAACTATTGGAGCGGTAAAC

Downstream 100 bases:

>100_bases
AGGTGGAATATGGAAAGGAAGCTGGCTTTTTTGTCAGCGTTTTCTTCTTGACTGACCGCTTCCTTATGAATACGCTTATG
ATAGTTAAGTTCAAGAAATG

Product: membrane endopeptidase

Products: NA

Alternate protein names: Intramembrane serine protease

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MFLLEYTYWKIAAHLVNSGYGVIQAGESDEIWLEAPDKSSHDLVRLYKHDLDFRQEMVRDIEEQAERVERVRHQLGRRRM
KLLNVFFSTEAPVDDWEEIAKKTFEKGTVSVEPAIVRGTMLRDDLQAVFPSFRTEDCSEEHASFENAQMARERFLSLVLK
QEEQRKTEAAVFQNGKPTFTYLFIALQILMFFLLEINGGSTNTETLVAFGAKENSLIAQGEWWRLLTPIVLHIGIAHLAF
NTLALWSVGTAVERMYGSGRFLLIYLAAGITGSIASFVFSPYPSAGASGAIFGCLGALLYVALSNRKMFLRTIGTNIIVI
IIINLGFGFAVSNIDNSGHIGGLIGGFFAAAALGLPKAGAFGKRLLSAVLLIALAVGFLYYGLHSPSHQESALIQQASEL
YQEGKYEEVTELLNGEAAQKDASADLLKILAVSDIQIGEYDQAVSLLERAVKKEPKDHASYYNLALLYAEKNELAQAEKA
IQTAVKLKPKEQRYKELQRQIENNKES

Sequences:

>Translated_507_residues
MFLLEYTYWKIAAHLVNSGYGVIQAGESDEIWLEAPDKSSHDLVRLYKHDLDFRQEMVRDIEEQAERVERVRHQLGRRRM
KLLNVFFSTEAPVDDWEEIAKKTFEKGTVSVEPAIVRGTMLRDDLQAVFPSFRTEDCSEEHASFENAQMARERFLSLVLK
QEEQRKTEAAVFQNGKPTFTYLFIALQILMFFLLEINGGSTNTETLVAFGAKENSLIAQGEWWRLLTPIVLHIGIAHLAF
NTLALWSVGTAVERMYGSGRFLLIYLAAGITGSIASFVFSPYPSAGASGAIFGCLGALLYVALSNRKMFLRTIGTNIIVI
IIINLGFGFAVSNIDNSGHIGGLIGGFFAAAALGLPKAGAFGKRLLSAVLLIALAVGFLYYGLHSPSHQESALIQQASEL
YQEGKYEEVTELLNGEAAQKDASADLLKILAVSDIQIGEYDQAVSLLERAVKKEPKDHASYYNLALLYAEKNELAQAEKA
IQTAVKLKPKEQRYKELQRQIENNKES
>Mature_507_residues
MFLLEYTYWKIAAHLVNSGYGVIQAGESDEIWLEAPDKSSHDLVRLYKHDLDFRQEMVRDIEEQAERVERVRHQLGRRRM
KLLNVFFSTEAPVDDWEEIAKKTFEKGTVSVEPAIVRGTMLRDDLQAVFPSFRTEDCSEEHASFENAQMARERFLSLVLK
QEEQRKTEAAVFQNGKPTFTYLFIALQILMFFLLEINGGSTNTETLVAFGAKENSLIAQGEWWRLLTPIVLHIGIAHLAF
NTLALWSVGTAVERMYGSGRFLLIYLAAGITGSIASFVFSPYPSAGASGAIFGCLGALLYVALSNRKMFLRTIGTNIIVI
IIINLGFGFAVSNIDNSGHIGGLIGGFFAAAALGLPKAGAFGKRLLSAVLLIALAVGFLYYGLHSPSHQESALIQQASEL
YQEGKYEEVTELLNGEAAQKDASADLLKILAVSDIQIGEYDQAVSLLERAVKKEPKDHASYYNLALLYAEKNELAQAEKA
IQTAVKLKPKEQRYKELQRQIENNKES

Specific function: Rhomboid-type serine protease that catalyzes intramembrane proteolysis. Important for normal cell division and sporulation. May act as a glucose exporter

COG id: COG0705

COG function: function code R; Uncharacterized membrane protein (homolog of Drosophila rhomboid)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 TPR repeats

Homologues:

Organism=Homo sapiens, GI190341097, Length=146, Percent_Identity=31.5068493150685, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI133901888, Length=146, Percent_Identity=32.1917808219178, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI115532986, Length=146, Percent_Identity=32.1917808219178, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI28575000, Length=182, Percent_Identity=26.9230769230769, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28575002, Length=182, Percent_Identity=26.9230769230769, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLUP_BACSU (P54493)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- PIR:   B69957
- RefSeq:   NP_390367.2
- ProteinModelPortal:   P54493
- MEROPS:   S54.014
- EnsemblBacteria:   EBBACT00000002100
- GeneID:   938211
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU24870
- NMPDR:   fig|224308.1.peg.2491
- GenoList:   BSU24870
- GeneTree:   EBGT00050000001978
- HOGENOM:   HBG345594
- ProtClustDB:   CLSK873020
- BioCyc:   BSUB:BSU24870-MONOMER
- BRENDA:   3.4.21.105
- GO:   GO:0005488
- InterPro:   IPR002610
- InterPro:   IPR022764
- InterPro:   IPR001440
- InterPro:   IPR013026
- InterPro:   IPR011990
- InterPro:   IPR019734
- Gene3D:   G3DSA:1.25.40.10
- PANTHER:   PTHR22936

Pfam domain/function: PF01694 Rhomboid; PF00515 TPR_1

EC number: =3.4.21.105

Molecular weight: Translated: 56462; Mature: 56462

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS50005 TPR; PS50293 TPR_REGION

Important sites: ACT_SITE 288-288 ACT_SITE 339-339

Signals:

None

Transmembrane regions:

HASH(0x10572a90)-; HASH(0xe633648)-; HASH(0x100da124)-; HASH(0x10a7b2d0)-; HASH(0x108647a4)-; HASH(0x10d058d0)-; HASH(0x1006c950)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MFLLEYTYWKIAAHLVNSGYGVIQAGESDEIWLEAPDKSSHDLVRLYKHDLDFRQEMVRD
CEEEEHHHHHHHHHHHHCCCCEEECCCCCCEEEECCCCCCHHHHHHHHHCHHHHHHHHHH
IEEQAERVERVRHQLGRRRMKLLNVFFSTEAPVDDWEEIAKKTFEKGTVSVEPAIVRGTM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEECCHHHHHHH
LRDDLQAVFPSFRTEDCSEEHASFENAQMARERFLSLVLKQEEQRKTEAAVFQNGKPTFT
HHHHHHHHCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
YLFIALQILMFFLLEINGGSTNTETLVAFGAKENSLIAQGEWWRLLTPIVLHIGIAHLAF
HHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHHH
NTLALWSVGTAVERMYGSGRFLLIYLAAGITGSIASFVFSPYPSAGASGAIFGCLGALLY
HHHHHHHHHHHHHHHHCCCCEEEEEEHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
VALSNRKMFLRTIGTNIIVIIIINLGFGFAVSNIDNSGHIGGLIGGFFAAAALGLPKAGA
HHHCCCHHEEHHHCCCEEEEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCH
FGKRLLSAVLLIALAVGFLYYGLHSPSHQESALIQQASELYQEGKYEEVTELLNGEAAQK
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHCC
DASADLLKILAVSDIQIGEYDQAVSLLERAVKKEPKDHASYYNLALLYAEKNELAQAEKA
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHEEEEEEECHHHHHHHHHH
IQTAVKLKPKEQRYKELQRQIENNKES
HHHHHHCCCHHHHHHHHHHHHHCCCCH
>Mature Secondary Structure
MFLLEYTYWKIAAHLVNSGYGVIQAGESDEIWLEAPDKSSHDLVRLYKHDLDFRQEMVRD
CEEEEHHHHHHHHHHHHCCCCEEECCCCCCEEEECCCCCCHHHHHHHHHCHHHHHHHHHH
IEEQAERVERVRHQLGRRRMKLLNVFFSTEAPVDDWEEIAKKTFEKGTVSVEPAIVRGTM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEECCHHHHHHH
LRDDLQAVFPSFRTEDCSEEHASFENAQMARERFLSLVLKQEEQRKTEAAVFQNGKPTFT
HHHHHHHHCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
YLFIALQILMFFLLEINGGSTNTETLVAFGAKENSLIAQGEWWRLLTPIVLHIGIAHLAF
HHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHHH
NTLALWSVGTAVERMYGSGRFLLIYLAAGITGSIASFVFSPYPSAGASGAIFGCLGALLY
HHHHHHHHHHHHHHHHCCCCEEEEEEHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
VALSNRKMFLRTIGTNIIVIIIINLGFGFAVSNIDNSGHIGGLIGGFFAAAALGLPKAGA
HHHCCCHHEEHHHCCCEEEEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCH
FGKRLLSAVLLIALAVGFLYYGLHSPSHQESALIQQASELYQEGKYEEVTELLNGEAAQK
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHCC
DASADLLKILAVSDIQIGEYDQAVSLLERAVKKEPKDHASYYNLALLYAEKNELAQAEKA
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHEEEEEEECHHHHHHHHHH
IQTAVKLKPKEQRYKELQRQIENNKES
HHHHHHCCCHHHHHHHHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377