Definition Bacillus subtilis subsp. subtilis str. 168 chromosome, complete genome.
Accession NC_000964
Length 4,215,606

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The map label for this gene is ispA

Identifier: 255767538

GI number: 255767538

Start: 2525789

End: 2526679

Strand: Reverse

Name: ispA

Synonym: BSU24280

Alternate gene names: 255767538

Gene position: 2526679-2525789 (Counterclockwise)

Preceding gene: 255767539

Following gene: 16079483

Centisome position: 59.94

GC content: 46.02

Gene sequence:

>891_bases
GTGACAAATAAATTAACGAGCTTTCTGGCGGACCGGAAAAAAACAATTGAAAATCAGCTTTCTGTCTATACAGAAAAGCT
TGATATGCCGGACTCATTAAAGAAATCTATGCTATATTCTCTACAGGCCGGCGGAAAGCGGTTGCGGCCTCTGATTGTAC
TGGCTGTTTTAAATGCATATGGAAAAAGCGAAAAAGACGGCATTCCGGTGGGCTGTGCTGTCGAAATGATTCACACGTAT
TCGTTAATTCATGATGATCTTCCATGCATGGATGATGACGATTTGCGCCGCGGGAAGCCGACAAACCATAAAGTGTTTGG
TGAAGCGACGGCAGTATTAGCGGGTGACGGGCTGCTCACAGAAAGCTTTAAGCTGATTACCTCCCACGTGTCAGACGAGG
TGTCAGCAGAAAAGCGCCTGCGGCTTGTGAATGAACTGATTTCAGCGGCAGGCACCGAAGGCATGGTCGGTGGGCAAGTA
GCTGATATGGAAGCGGAAAACCGACAAGTCACGCTTGAAGAGCTCGAATCCATTCATGAACGGAAAACTGCCAAGCTCCT
TGGCTTTTGTGTAATCGCCGGTGCTATTTTGGCTGATGCGCCTGAGGAAGACATTGAAACACTGCGTACCTTCAGCAGCC
ACATTGGAATCGGATTTCAAATCAGAGACGATATTTTAGATTTAGAAGGCAGTGAAGAGAAAATCGGCAAACGTGTCGGC
TCGGATACCACAAATGACAAATCGACATACCCGTCGCTTCTTTCATTGGAAGGGGCCAAACATAAATTGGATGTTCATAT
AAAAGAGGCGAAGCGATTGATCGGCGGACTCTCTCTTCAAAAAGACCTTTTATATGAGCTTTGTGATTTAATTGCGGCAA
GAGATCACTAA

Upstream 100 bases:

>100_bases
ATGTGCCATGAAAAGCTGCAAAAAGTCGAAAAGCAAATGGACTTCATTTTAAAAGAAGACGGCGAACTGGCACCTTTCAG
TGTTCAGGAGGAAGACGAAG

Downstream 100 bases:

>100_bases
AACGATCCTAATTTGAAGCGGCTGCACGAGGTATGGTAAAATGTAAGCAGTTTATATGAAAAATGAAAAGGAAAAGCGCG
TTTGTCTTTTTTTATGTCTG

Product: geranyltranstransferase

Products: NA

Alternate protein names: FPP synthase; (2E,6E)-farnesyl diphosphate synthase; Geranyltranstransferase

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MTNKLTSFLADRKKTIENQLSVYTEKLDMPDSLKKSMLYSLQAGGKRLRPLIVLAVLNAYGKSEKDGIPVGCAVEMIHTY
SLIHDDLPCMDDDDLRRGKPTNHKVFGEATAVLAGDGLLTESFKLITSHVSDEVSAEKRLRLVNELISAAGTEGMVGGQV
ADMEAENRQVTLEELESIHERKTAKLLGFCVIAGAILADAPEEDIETLRTFSSHIGIGFQIRDDILDLEGSEEKIGKRVG
SDTTNDKSTYPSLLSLEGAKHKLDVHIKEAKRLIGGLSLQKDLLYELCDLIAARDH

Sequences:

>Translated_296_residues
MTNKLTSFLADRKKTIENQLSVYTEKLDMPDSLKKSMLYSLQAGGKRLRPLIVLAVLNAYGKSEKDGIPVGCAVEMIHTY
SLIHDDLPCMDDDDLRRGKPTNHKVFGEATAVLAGDGLLTESFKLITSHVSDEVSAEKRLRLVNELISAAGTEGMVGGQV
ADMEAENRQVTLEELESIHERKTAKLLGFCVIAGAILADAPEEDIETLRTFSSHIGIGFQIRDDILDLEGSEEKIGKRVG
SDTTNDKSTYPSLLSLEGAKHKLDVHIKEAKRLIGGLSLQKDLLYELCDLIAARDH
>Mature_295_residues
TNKLTSFLADRKKTIENQLSVYTEKLDMPDSLKKSMLYSLQAGGKRLRPLIVLAVLNAYGKSEKDGIPVGCAVEMIHTYS
LIHDDLPCMDDDDLRRGKPTNHKVFGEATAVLAGDGLLTESFKLITSHVSDEVSAEKRLRLVNELISAAGTEGMVGGQVA
DMEAENRQVTLEELESIHERKTAKLLGFCVIAGAILADAPEEDIETLRTFSSHIGIGFQIRDDILDLEGSEEKIGKRVGS
DTTNDKSTYPSLLSLEGAKHKLDVHIKEAKRLIGGLSLQKDLLYELCDLIAARDH

Specific function: Unknown

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family

Homologues:

Organism=Homo sapiens, GI50659086, Length=236, Percent_Identity=30.0847457627119, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1786623, Length=287, Percent_Identity=42.8571428571429, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1789578, Length=218, Percent_Identity=32.5688073394495, Blast_Score=116, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17505681, Length=263, Percent_Identity=28.1368821292776, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24651612, Length=227, Percent_Identity=29.0748898678414, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPA_BACSU (P54383)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- PIR:   A69961
- RefSeq:   NP_390308.2
- ProteinModelPortal:   P54383
- SMR:   P54383
- EnsemblBacteria:   EBBACT00000000925
- GeneID:   938652
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU24280
- NMPDR:   fig|224308.1.peg.2432
- GenoList:   BSU24280
- GeneTree:   EBGT00050000001433
- HOGENOM:   HBG732667
- PhylomeDB:   P54383
- ProtClustDB:   CLSK887549
- BioCyc:   BSUB:BSU24280-MONOMER
- BRENDA:   2.5.1.10
- GO:   GO:0005737
- InterPro:   IPR000092
- InterPro:   IPR008949
- Gene3D:   G3DSA:1.10.600.10

Pfam domain/function: PF00348 polyprenyl_synt; SSF48576 Terpenoid_synth

EC number: =2.5.1.10

Molecular weight: Translated: 32504; Mature: 32372

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00723 POLYPRENYL_SYNTHASE_1; PS00444 POLYPRENYL_SYNTHASE_2

Important sites: BINDING 46-46 BINDING 49-49 BINDING 78-78 BINDING 96-96 BINDING 97-97 BINDING 182-182 BINDING 183-183 BINDING 220-220 BINDING 237-237

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MTNKLTSFLADRKKTIENQLSVYTEKLDMPDSLKKSMLYSLQAGGKRLRPLIVLAVLNAY
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
GKSEKDGIPVGCAVEMIHTYSLIHDDLPCMDDDDLRRGKPTNHKVFGEATAVLAGDGLLT
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCEECCCHHEEEECCCHHH
ESFKLITSHVSDEVSAEKRLRLVNELISAAGTEGMVGGQVADMEAENRQVTLEELESIHE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCEEEHHHHHHHHH
RKTAKLLGFCVIAGAILADAPEEDIETLRTFSSHIGIGFQIRDDILDLEGSEEKIGKRVG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCHHHHHHHHC
SDTTNDKSTYPSLLSLEGAKHKLDVHIKEAKRLIGGLSLQKDLLYELCDLIAARDH
CCCCCCCHHCCHHHHCCCCCHHHEEHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TNKLTSFLADRKKTIENQLSVYTEKLDMPDSLKKSMLYSLQAGGKRLRPLIVLAVLNAY
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
GKSEKDGIPVGCAVEMIHTYSLIHDDLPCMDDDDLRRGKPTNHKVFGEATAVLAGDGLLT
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCEECCCHHEEEECCCHHH
ESFKLITSHVSDEVSAEKRLRLVNELISAAGTEGMVGGQVADMEAENRQVTLEELESIHE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCEEEHHHHHHHHH
RKTAKLLGFCVIAGAILADAPEEDIETLRTFSSHIGIGFQIRDDILDLEGSEEKIGKRVG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCHHHHHHHHC
SDTTNDKSTYPSLLSLEGAKHKLDVHIKEAKRLIGGLSLQKDLLYELCDLIAARDH
CCCCCCCHHCCHHHHCCCCCHHHEEHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377