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The map label for this gene is recN

Identifier: 255767536

GI number: 255767536

Start: 2520557

End: 2522287

Strand: Reverse

Name: recN

Synonym: BSU24240

Alternate gene names: 255767536

Gene position: 2522287-2520557 (Counterclockwise)

Preceding gene: 16079481

Following gene: 255767535

Centisome position: 59.83

GC content: 45.75

Gene sequence:

>1731_bases
TTGTTAGCTGAACTATCGATTAAAAACTTTGCCATTATTGAGGAACTGACGGTTTCTTTTGAACGCGGGCTTACGGTTTT
AACAGGAGAAACCGGAGCCGGCAAATCCATCATCATAGATGCCATTTCTCTTTTAGTCGGAGGCCGCGGATCATCTGAAT
TTGTTAGATATGGGGAAGCAAAAGCCGAACTTGAAGGGCTGTTTCTGTTGGAGAGCGGCCATCCTGTTTTAGGCGTATGC
GCTGAGCAGGGAATCGACGTTTCAGACGAAATGATCGTCATGAGAAGGGATATCAGCACGAGCGGGAAAAGCGTTTGCCG
TGTCAATGGCAAGCTTGTCACGATTGCTTCACTAAGAGAGATCGGCAGGCTTTTATTAGATATTCACGGGCAGCACGATA
ACCAGCTGTTAATGGAAGACGAAAATCATCTCCAGCTTTTGGACAAGTTTGCCGGGGCCGAAGTGGAAAGCGCGCTCAAA
ACGTATCAGGAAGGGTACCAGCGGTATGTGAAGCTGCTCAAAAAGCTTAAGCAGCTTTCTGAAAGCGAACAGGAAATGGC
CCATCGTCTTGATCTTATTCAATTTCAGCTCGAAGAAATCGAATCGGCAAAGCTTGAGCTTAACGAAGATGAACAGCTCC
AAGAAGAGCGCCAGCAAATTTCAAACTTTGAGAAGATATATGAGTCGCTGCAAAATGCATATAACGCGCTTCGCAGTGAG
CAGGGCGGATTGGACTGGGTTGGAATGGCATCAGCGCAGCTTGAAGATATATCTGATATAAACGAACCGCTGAAAAAAAT
GTCTGAAAGTGTTTCGAACTCCTACTATTTGCTTGAGGATGCCACATTTCAGATGAGAAATATGCTGGATGAGCTTGAAT
TTGATCCGGAACGCCTGAACTATATTGAGACGCGACTTAATGAAATCAAACAGCTGAAACGAAAATACGGTGCCACTGTT
GAAGACATTTTGGAATATGCTTCTAAAATTGAAGAAGAGATTGACCAAATTGAAAACAGGGACAGCCACCTTCAAAGCTT
GAAAAAGGAGCTTGACTCCGTCGGCAAAGATGTCGCAGTGGAAGCGGCAAATGTGTCACAAATCCGAAAAACATGGGCGA
AAAAGCTGGCTGATGAGATTCATCGGGAATTAAAAAGCCTGTACATGGAAAAATCAACTTTTGATACGGAATTTAAGGTC
AGAACAGCGTCACGCAATGAAGAGGCGCCGCTTGTAAACGGCCAGCCCGTTCAGCTGACTGAACAAGGGATTGACCTGGT
GAAGTTTTTAATTTCAACCAATACAGGCGAGCCGCTGAAATCCCTTTCGAAAGTCGCTTCAGGTGGAGAGCTGTCACGGG
TGATGCTTGCCATAAAAAGCATTTTTTCCTCACAACAGGATGTGACATCGATCATTTTTGACGAGGTTGATACCGGTGTA
AGCGGCAGGGTCGCACAGGCAATTGCTGAGAAGATCCATAAGGTGTCAATAGGATCACAAGTGCTGTGCATTACACACCT
GCCTCAGGTGGCTGCCATGGCCGATACGCATCTTTATATTGCGAAGGAGTTAAAAGACGGCAGAACGACGACACGTGTCA
AGCCGCTCTCTAAGCAGGAAAAGGTAGCGGAAATCGGGCGCATGATTGCAGGTGTCGAAGTAACCGACTTAACGAAACGC
CATGCGAAAGAACTTTTAAAACAAGCGGATCAAGTCAAAACAACTGGGTAA

Upstream 100 bases:

>100_bases
AATTATTTGCCGGACTCCTGAAGATACAGAAGGCGTAAAAAACAGGCTCCTTGAACTGCTGTAACAGAAAATCTAACAAA
GATAAGAGGTGTAATGCTGT

Downstream 100 bases:

>100_bases
GCTGCGCGAGAAGCGCAGCTTATTTTTTTCGTGCACATCCATTCGTTCATCAGTATATCCAATGTTTTTCTTCATATGAC
AGTTATAAATAAGCCGTCAG

Product: factor for double strand breaks DNA repair and genetic recombination

Products: NA

Alternate protein names: Recombination protein N

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MLAELSIKNFAIIEELTVSFERGLTVLTGETGAGKSIIIDAISLLVGGRGSSEFVRYGEAKAELEGLFLLESGHPVLGVC
AEQGIDVSDEMIVMRRDISTSGKSVCRVNGKLVTIASLREIGRLLLDIHGQHDNQLLMEDENHLQLLDKFAGAEVESALK
TYQEGYQRYVKLLKKLKQLSESEQEMAHRLDLIQFQLEEIESAKLELNEDEQLQEERQQISNFEKIYESLQNAYNALRSE
QGGLDWVGMASAQLEDISDINEPLKKMSESVSNSYYLLEDATFQMRNMLDELEFDPERLNYIETRLNEIKQLKRKYGATV
EDILEYASKIEEEIDQIENRDSHLQSLKKELDSVGKDVAVEAANVSQIRKTWAKKLADEIHRELKSLYMEKSTFDTEFKV
RTASRNEEAPLVNGQPVQLTEQGIDLVKFLISTNTGEPLKSLSKVASGGELSRVMLAIKSIFSSQQDVTSIIFDEVDTGV
SGRVAQAIAEKIHKVSIGSQVLCITHLPQVAAMADTHLYIAKELKDGRTTTRVKPLSKQEKVAEIGRMIAGVEVTDLTKR
HAKELLKQADQVKTTG

Sequences:

>Translated_576_residues
MLAELSIKNFAIIEELTVSFERGLTVLTGETGAGKSIIIDAISLLVGGRGSSEFVRYGEAKAELEGLFLLESGHPVLGVC
AEQGIDVSDEMIVMRRDISTSGKSVCRVNGKLVTIASLREIGRLLLDIHGQHDNQLLMEDENHLQLLDKFAGAEVESALK
TYQEGYQRYVKLLKKLKQLSESEQEMAHRLDLIQFQLEEIESAKLELNEDEQLQEERQQISNFEKIYESLQNAYNALRSE
QGGLDWVGMASAQLEDISDINEPLKKMSESVSNSYYLLEDATFQMRNMLDELEFDPERLNYIETRLNEIKQLKRKYGATV
EDILEYASKIEEEIDQIENRDSHLQSLKKELDSVGKDVAVEAANVSQIRKTWAKKLADEIHRELKSLYMEKSTFDTEFKV
RTASRNEEAPLVNGQPVQLTEQGIDLVKFLISTNTGEPLKSLSKVASGGELSRVMLAIKSIFSSQQDVTSIIFDEVDTGV
SGRVAQAIAEKIHKVSIGSQVLCITHLPQVAAMADTHLYIAKELKDGRTTTRVKPLSKQEKVAEIGRMIAGVEVTDLTKR
HAKELLKQADQVKTTG
>Mature_576_residues
MLAELSIKNFAIIEELTVSFERGLTVLTGETGAGKSIIIDAISLLVGGRGSSEFVRYGEAKAELEGLFLLESGHPVLGVC
AEQGIDVSDEMIVMRRDISTSGKSVCRVNGKLVTIASLREIGRLLLDIHGQHDNQLLMEDENHLQLLDKFAGAEVESALK
TYQEGYQRYVKLLKKLKQLSESEQEMAHRLDLIQFQLEEIESAKLELNEDEQLQEERQQISNFEKIYESLQNAYNALRSE
QGGLDWVGMASAQLEDISDINEPLKKMSESVSNSYYLLEDATFQMRNMLDELEFDPERLNYIETRLNEIKQLKRKYGATV
EDILEYASKIEEEIDQIENRDSHLQSLKKELDSVGKDVAVEAANVSQIRKTWAKKLADEIHRELKSLYMEKSTFDTEFKV
RTASRNEEAPLVNGQPVQLTEQGIDLVKFLISTNTGEPLKSLSKVASGGELSRVMLAIKSIFSSQQDVTSIIFDEVDTGV
SGRVAQAIAEKIHKVSIGSQVLCITHLPQVAAMADTHLYIAKELKDGRTTTRVKPLSKQEKVAEIGRMIAGVEVTDLTKR
HAKELLKQADQVKTTG

Specific function: Involved in recombinational repair of damaged DNA. Seems to be the first protein recruited to repair centers, foci that are the site of double-strand DNA break(s), followed by recO and then recF

COG id: COG0497

COG function: function code L; ATPase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recN family

Homologues:

Organism=Escherichia coli, GI48994901, Length=573, Percent_Identity=34.7294938917976, Blast_Score=312, Evalue=4e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECN_BACSU (P17894)

Other databases:

- EMBL:   M30297
- EMBL:   D84432
- EMBL:   AL009126
- EMBL:   M27869
- EMBL:   U68235
- PIR:   B35128
- RefSeq:   NP_390304.2
- ProteinModelPortal:   P17894
- EnsemblBacteria:   EBBACT00000000481
- GeneID:   938656
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU24240
- NMPDR:   fig|224308.1.peg.2428
- GenoList:   BSU24240
- GeneTree:   EBGT00050000000246
- HOGENOM:   HBG632212
- ProtClustDB:   CLSK887548
- BioCyc:   BSUB:BSU24240-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- InterPro:   IPR004604
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00634

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 64509; Mature: 64509

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAELSIKNFAIIEELTVSFERGLTVLTGETGAGKSIIIDAISLLVGGRGSSEFVRYGEA
CCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCH
KAELEGLFLLESGHPVLGVCAEQGIDVSDEMIVMRRDISTSGKSVCRVNGKLVTIASLRE
HHHHCEEEEEECCCCEEEHHHHCCCCCCHHHEEEECCCCCCCCCEEEECCCEEEHHHHHH
IGRLLLDIHGQHDNQLLMEDENHLQLLDKFAGAEVESALKTYQEGYQRYVKLLKKLKQLS
HHHHHHHHCCCCCCCEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESEQEMAHRLDLIQFQLEEIESAKLELNEDEQLQEERQQISNFEKIYESLQNAYNALRSE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QGGLDWVGMASAQLEDISDINEPLKKMSESVSNSYYLLEDATFQMRNMLDELEFDPERLN
CCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHCCCHHHHH
YIETRLNEIKQLKRKYGATVEDILEYASKIEEEIDQIENRDSHLQSLKKELDSVGKDVAV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEE
EAANVSQIRKTWAKKLADEIHRELKSLYMEKSTFDTEFKVRTASRNEEAPLVNGQPVQLT
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHH
EQGIDLVKFLISTNTGEPLKSLSKVASGGELSRVMLAIKSIFSSQQDVTSIIFDEVDTGV
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
SGRVAQAIAEKIHKVSIGSQVLCITHLPQVAAMADTHLYIAKELKDGRTTTRVKPLSKQE
CHHHHHHHHHHHHHHCCCCCEEEEHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
KVAEIGRMIAGVEVTDLTKRHAKELLKQADQVKTTG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLAELSIKNFAIIEELTVSFERGLTVLTGETGAGKSIIIDAISLLVGGRGSSEFVRYGEA
CCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCH
KAELEGLFLLESGHPVLGVCAEQGIDVSDEMIVMRRDISTSGKSVCRVNGKLVTIASLRE
HHHHCEEEEEECCCCEEEHHHHCCCCCCHHHEEEECCCCCCCCCEEEECCCEEEHHHHHH
IGRLLLDIHGQHDNQLLMEDENHLQLLDKFAGAEVESALKTYQEGYQRYVKLLKKLKQLS
HHHHHHHHCCCCCCCEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESEQEMAHRLDLIQFQLEEIESAKLELNEDEQLQEERQQISNFEKIYESLQNAYNALRSE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QGGLDWVGMASAQLEDISDINEPLKKMSESVSNSYYLLEDATFQMRNMLDELEFDPERLN
CCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHCCCHHHHH
YIETRLNEIKQLKRKYGATVEDILEYASKIEEEIDQIENRDSHLQSLKKELDSVGKDVAV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEE
EAANVSQIRKTWAKKLADEIHRELKSLYMEKSTFDTEFKVRTASRNEEAPLVNGQPVQLT
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHH
EQGIDLVKFLISTNTGEPLKSLSKVASGGELSRVMLAIKSIFSSQQDVTSIIFDEVDTGV
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
SGRVAQAIAEKIHKVSIGSQVLCITHLPQVAAMADTHLYIAKELKDGRTTTRVKPLSKQE
CHHHHHHHHHHHHHHCCCCCEEEEHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
KVAEIGRMIAGVEVTDLTKRHAKELLKQADQVKTTG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 2507400; 9044256