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The map label for this gene is xerD

Identifier: 255767509

GI number: 255767509

Start: 2448591

End: 2449481

Strand: Reverse

Name: xerD

Synonym: BSU23510

Alternate gene names: 255767509

Gene position: 2449481-2448591 (Counterclockwise)

Preceding gene: 255767510

Following gene: 255767508

Centisome position: 58.11

GC content: 45.45

Gene sequence:

>891_bases
GTGAAAGATCAAATAAAGGATTTCATCCACTACGTTATGGTAGAGAGAGGGCTCTCTCAAAATACGATCGTATCCTATGA
ACGTGACTTGAAAAGCTATTCTCTCTACTTAACAGAAACCCTTCATGTTACAGATTGGAATCACGTTACCCGCATACATA
TTATCCAATACTTAAAGCATTTGAAGGACTCGGGGAAATCCGGCAAGACATCGGCGCGGCATTTGGCATCCATTCGCTCT
TTTCATCAATTTCTGCTCAGGGAAAAAGTAACGGATAAGGACCCTTCCGTTCATATCGAAACACAAAAGACGGAACGGGC
GCTTCCGAAGGTGCTCGCGCTCAATGAAGTCGAGCGGTTATTGGACACGCCTAAGCTGACGAGCCCTTTCGGATATCGGG
ATAAAGCAATGCTGGAGCTGCTTTATGCGACAGGCATACGAGTCAGTGAAATGATTGAATTGAAAACGGCTGATGTTCAT
TTATCTATGGGGTTTATCCGATGCTTCGGCAAAGGAAGAAAAGAACGGATTGTGCCAATCGGTGAAGCCGCTGCTTCCGC
CATTGAGGAATATATGACAAAAGCCAGGGGAAAACTGTTAAAAAATAATGTATCTGACGCCCTCTTTTTAAATCATCACG
GCAAACAGATCAGCCGCCAGGGATTTTGGAAGAACCTGAAAAAAATCGCATTGGAAGCCGGCATTAAGAAAGAGCTGACT
CCGCATACACTCAGGCATTCCTTTGCGACGCATCTGCTTGAGAACGGGGCAGATTTAAGAGCGGTGCAGGAAATGCTCGG
TCATGCGGATATATCCACGACGCAAATTTATACGCATGTGACGAAAACAAGGCTGAAGGATGTGTACAAGCAGTTTCATC
CGCGAGCATAG

Upstream 100 bases:

>100_bases
GAAGGCTCCGCTGTAAAAGTTGTTGAAATGGATCAGGATGAAAAAGGCGGATGGTTCGACCGGTTGATCTTTTTTTACCA
AAACGGGGAGTAGATCAAGA

Downstream 100 bases:

>100_bases
CCGCAGGTGGCTGCGGCTTTTTTGTTTAGAGCGACAGAAAATATTGAAGTTTCTCATTATTTCCAAATACATTGTCAACG
GGAATGAAAACGGTTTATAC

Product: site-specific tyrosine recombinase XerD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MKDQIKDFIHYVMVERGLSQNTIVSYERDLKSYSLYLTETLHVTDWNHVTRIHIIQYLKHLKDSGKSGKTSARHLASIRS
FHQFLLREKVTDKDPSVHIETQKTERALPKVLALNEVERLLDTPKLTSPFGYRDKAMLELLYATGIRVSEMIELKTADVH
LSMGFIRCFGKGRKERIVPIGEAAASAIEEYMTKARGKLLKNNVSDALFLNHHGKQISRQGFWKNLKKIALEAGIKKELT
PHTLRHSFATHLLENGADLRAVQEMLGHADISTTQIYTHVTKTRLKDVYKQFHPRA

Sequences:

>Translated_296_residues
MKDQIKDFIHYVMVERGLSQNTIVSYERDLKSYSLYLTETLHVTDWNHVTRIHIIQYLKHLKDSGKSGKTSARHLASIRS
FHQFLLREKVTDKDPSVHIETQKTERALPKVLALNEVERLLDTPKLTSPFGYRDKAMLELLYATGIRVSEMIELKTADVH
LSMGFIRCFGKGRKERIVPIGEAAASAIEEYMTKARGKLLKNNVSDALFLNHHGKQISRQGFWKNLKKIALEAGIKKELT
PHTLRHSFATHLLENGADLRAVQEMLGHADISTTQIYTHVTKTRLKDVYKQFHPRA
>Mature_296_residues
MKDQIKDFIHYVMVERGLSQNTIVSYERDLKSYSLYLTETLHVTDWNHVTRIHIIQYLKHLKDSGKSGKTSARHLASIRS
FHQFLLREKVTDKDPSVHIETQKTERALPKVLALNEVERLLDTPKLTSPFGYRDKAMLELLYATGIRVSEMIELKTADVH
LSMGFIRCFGKGRKERIVPIGEAAASAIEEYMTKARGKLLKNNVSDALFLNHHGKQISRQGFWKNLKKIALEAGIKKELT
PHTLRHSFATHLLENGADLRAVQEMLGHADISTTQIYTHVTKTRLKDVYKQFHPRA

Specific function: Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. The xerC-xerD complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell div

COG id: COG4974

COG function: function code L; Site-specific recombinase XerD

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family. XerD subfamily

Homologues:

Organism=Escherichia coli, GI1789261, Length=296, Percent_Identity=44.5945945945946, Blast_Score=249, Evalue=1e-67,
Organism=Escherichia coli, GI1790244, Length=292, Percent_Identity=35.958904109589, Blast_Score=194, Evalue=5e-51,
Organism=Escherichia coli, GI1790768, Length=163, Percent_Identity=31.2883435582822, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790767, Length=186, Percent_Identity=27.9569892473118, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): XERD_BACSU (P46352)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- EMBL:   U32685
- PIR:   A69693
- RefSeq:   NP_390232.2
- ProteinModelPortal:   P46352
- SMR:   P46352
- EnsemblBacteria:   EBBACT00000000556
- GeneID:   938726
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU23510
- NMPDR:   fig|224308.1.peg.2356
- GenoList:   BSU23510
- GeneTree:   EBGT00070000031883
- HOGENOM:   HBG727654
- PhylomeDB:   P46352
- ProtClustDB:   PRK00283
- BioCyc:   BSUB:BSU23510-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01807
- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR010998
- InterPro:   IPR023109
- InterPro:   IPR004107
- InterPro:   IPR011932
- Gene3D:   G3DSA:1.10.150.130
- Gene3D:   G3DSA:1.10.443.10
- TIGRFAMs:   TIGR02225

Pfam domain/function: PF02899 Phage_integr_N; PF00589 Phage_integrase; SSF56349 DNA_brk_join_enz; SSF47823 L_intgrse_like_N

EC number: NA

Molecular weight: Translated: 33995; Mature: 33995

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: NA

Important sites: ACT_SITE 147-147 ACT_SITE 171-171 ACT_SITE 242-242 ACT_SITE 245-245 ACT_SITE 268-268 ACT_SITE 277-277

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDQIKDFIHYVMVERGLSQNTIVSYERDLKSYSLYLTETLHVTDWNHVTRIHIIQYLKH
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEEEEEEECCCCHHHHHHHHHHHHH
LKDSGKSGKTSARHLASIRSFHQFLLREKVTDKDPSVHIETQKTERALPKVLALNEVERL
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHH
LDTPKLTSPFGYRDKAMLELLYATGIRVSEMIELKTADVHLSMGFIRCFGKGRKERIVPI
HCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GEAAASAIEEYMTKARGKLLKNNVSDALFLNHHGKQISRQGFWKNLKKIALEAGIKKELT
HHHHHHHHHHHHHHHHHHHHHCCCCHHHEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
PHTLRHSFATHLLENGADLRAVQEMLGHADISTTQIYTHVTKTRLKDVYKQFHPRA
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKDQIKDFIHYVMVERGLSQNTIVSYERDLKSYSLYLTETLHVTDWNHVTRIHIIQYLKH
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEEEEEEECCCCHHHHHHHHHHHHH
LKDSGKSGKTSARHLASIRSFHQFLLREKVTDKDPSVHIETQKTERALPKVLALNEVERL
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHH
LDTPKLTSPFGYRDKAMLELLYATGIRVSEMIELKTADVHLSMGFIRCFGKGRKERIVPI
HCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GEAAASAIEEYMTKARGKLLKNNVSDALFLNHHGKQISRQGFWKNLKKIALEAGIKKELT
HHHHHHHHHHHHHHHHHHHHHCCCCHHHEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
PHTLRHSFATHLLENGADLRAVQEMLGHADISTTQIYTHVTKTRLKDVYKQFHPRA
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10537218; 10498718