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The map label for this gene is rluB

Identifier: 255767501

GI number: 255767501

Start: 2421438

End: 2422172

Strand: Reverse

Name: rluB

Synonym: BSU23160

Alternate gene names: 255767501

Gene position: 2422172-2421438 (Counterclockwise)

Preceding gene: 255767502

Following gene: 255767500

Centisome position: 57.46

GC content: 47.07

Gene sequence:

>735_bases
ATGGAAAGACTGCAAAAAGTGATTGCTCATGCCGGAGTTGCGTCGCGCAGAAAGGCAGAAGAGCTGATTAAAGAAGGAAA
AGTAAAGGTGAATGGCAAAGTAGTAACAGAGCTAGGTGTTAAGGTCACCGGTTCTGATCAGATTGAGGTAAACGGGTTAA
AGGTTGAACGCGAAGAGCCGGTTTACTTTCTCTTATATAAACCAAGAGGCGTTATTTCCGCAGCGCAAGATGATAAAGGA
CGCAAAGTGGTGACGGACTTTTTTAAAAATATTCCGCAGCGCATTTATCCGATTGGGCGTCTGGACTATGATACGAGCGG
TCTTTTGCTTTTAACCAATGACGGCGAATTCGCCAATAAGCTAATGCATCCTAAGTATGAAATAGACAAAACATACGTGG
CGAAGGTGAAAGGAATCCCGCCTAAAGAGCTGCTCAGAAAGCTGGAGCGCGGAATTAGGCTGGAGGAAGGCAAAACAGCG
CCTGCTAAAGCGAAGCTGCTGTCTTTGGATAAGAAAAAGCAGACAAGCATTATTCAGCTGACGATCCATGAGGGACGCAA
CAGACAAGTGCGCCGCATGTTTGAAGCGATCGGACATGAGGTAATCAAGCTGAAGCGTGAGGAATACGCGTTTTTAAACC
TGCGAGGCCTGCACACGGGCGATGCCAGAGAGCTTACGCCTCACGAAGTAAAAAGGCTCAGAGCACTGGCGGATCACGGT
AAAAACGCTTTCTAA

Upstream 100 bases:

>100_bases
AGCGCCTGAAAACGGCGTTTTTTTTAGATTTGGCATAGGAATTTGAAAAAGCTTGTTTGTTAAGGTTGAAAAAAGTAAGA
TAATACTTGAGGTGAAAGAC

Downstream 100 bases:

>100_bases
ATTTCACATAACCTTCAAAAAGTAAGAAATGTGAAATGAACGTGCAATGATATAATTGAATGGATCTGCAATAGGGGGAA
GGGGGCAGTGGACAATGAAG

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MERLQKVIAHAGVASRRKAEELIKEGKVKVNGKVVTELGVKVTGSDQIEVNGLKVEREEPVYFLLYKPRGVISAAQDDKG
RKVVTDFFKNIPQRIYPIGRLDYDTSGLLLLTNDGEFANKLMHPKYEIDKTYVAKVKGIPPKELLRKLERGIRLEEGKTA
PAKAKLLSLDKKKQTSIIQLTIHEGRNRQVRRMFEAIGHEVIKLKREEYAFLNLRGLHTGDARELTPHEVKRLRALADHG
KNAF

Sequences:

>Translated_244_residues
MERLQKVIAHAGVASRRKAEELIKEGKVKVNGKVVTELGVKVTGSDQIEVNGLKVEREEPVYFLLYKPRGVISAAQDDKG
RKVVTDFFKNIPQRIYPIGRLDYDTSGLLLLTNDGEFANKLMHPKYEIDKTYVAKVKGIPPKELLRKLERGIRLEEGKTA
PAKAKLLSLDKKKQTSIIQLTIHEGRNRQVRRMFEAIGHEVIKLKREEYAFLNLRGLHTGDARELTPHEVKRLRALADHG
KNAF
>Mature_244_residues
MERLQKVIAHAGVASRRKAEELIKEGKVKVNGKVVTELGVKVTGSDQIEVNGLKVEREEPVYFLLYKPRGVISAAQDDKG
RKVVTDFFKNIPQRIYPIGRLDYDTSGLLLLTNDGEFANKLMHPKYEIDKTYVAKVKGIPPKELLRKLERGIRLEEGKTA
PAKAKLLSLDKKKQTSIIQLTIHEGRNRQVRRMFEAIGHEVIKLKREEYAFLNLRGLHTGDARELTPHEVKRLRALADHG
KNAF

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787524, Length=246, Percent_Identity=39.8373983739837, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1790453, Length=243, Percent_Identity=34.156378600823, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1788510, Length=235, Percent_Identity=29.7872340425532, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI87081838, Length=187, Percent_Identity=36.3636363636364, Blast_Score=108, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLUB_BACSU (P35159)

Other databases:

- EMBL:   L09228
- EMBL:   AL009126
- PIR:   S45555
- RefSeq:   NP_390197.2
- ProteinModelPortal:   P35159
- SMR:   P35159
- EnsemblBacteria:   EBBACT00000000371
- GeneID:   938955
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU23160
- NMPDR:   fig|224308.1.peg.2320
- GenoList:   BSU23160
- GeneTree:   EBGT00050000001923
- HOGENOM:   HBG754478
- PhylomeDB:   P35159
- ProtClustDB:   CLSK887511
- BioCyc:   BSUB:BSU23160-MONOMER
- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942
- SMART:   SM00363
- TIGRFAMs:   TIGR00093

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 27739; Mature: 27739

Theoretical pI: Translated: 10.51; Mature: 10.51

Prosite motif: PS01149 PSI_RSU; PS50889 S4

Important sites: ACT_SITE 102-102

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLQKVIAHAGVASRRKAEELIKEGKVKVNGKVVTELGVKVTGSDQIEVNGLKVEREEP
CHHHHHHHHHCCHHHHHHHHHHHHCCCEEECCEEEEEECEEECCCCEEEEECEEEECCCC
VYFLLYKPRGVISAAQDDKGRKVVTDFFKNIPQRIYPIGRLDYDTSGLLLLTNDGEFANK
EEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHH
LMHPKYEIDKTYVAKVKGIPPKELLRKLERGIRLEEGKTAPAKAKLLSLDKKKQTSIIQL
HCCCCHHCCHHEEEEECCCCHHHHHHHHHHCCEECCCCCCCHHHHHHHCCCCCCCEEEEE
TIHEGRNRQVRRMFEAIGHEVIKLKREEYAFLNLRGLHTGDARELTPHEVKRLRALADHG
EEECCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCC
KNAF
CCCC
>Mature Secondary Structure
MERLQKVIAHAGVASRRKAEELIKEGKVKVNGKVVTELGVKVTGSDQIEVNGLKVEREEP
CHHHHHHHHHCCHHHHHHHHHHHHCCCEEECCEEEEEECEEECCCCEEEEECEEEECCCC
VYFLLYKPRGVISAAQDDKGRKVVTDFFKNIPQRIYPIGRLDYDTSGLLLLTNDGEFANK
EEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHH
LMHPKYEIDKTYVAKVKGIPPKELLRKLERGIRLEEGKTAPAKAKLLSLDKKKQTSIIQL
HCCCCHHCCHHEEEEECCCCHHHHHHHHHHCCEECCCCCCCHHHHHHHCCCCCCCEEEEE
TIHEGRNRQVRRMFEAIGHEVIKLKREEYAFLNLRGLHTGDARELTPHEVKRLRALADHG
EEECCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCC
KNAF
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802