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The map label for this gene is trpF

Identifier: 255767487

GI number: 255767487

Start: 2373487

End: 2374134

Strand: Reverse

Name: trpF

Synonym: BSU22650

Alternate gene names: 255767487

Gene position: 2374134-2373487 (Counterclockwise)

Preceding gene: 255767488

Following gene: 50812257

Centisome position: 56.32

GC content: 44.29

Gene sequence:

>648_bases
GTGAAGAAACCGGCATTAAAATATTGCGGTATTCGGTCACTAAAGGATTTGCAGCTTGCGGCGGAATCACAGGCTGATTA
CCTAGGATTTATTTTTGCTGAAAGCAAACGAAAAGTATCTCCGGAAGATGTGAAAAAATGGCTGAACCAAGTTCGTGTCG
AAAAACAGGTTGCAGGTGTTTTTGTTAATGAATCAATAGAGACGATGTCACGTATTGCCAAGAGCTTGAAGCTCGACGTC
ATTCAGCTTCACGGTGATGAAAAACCGGCGGATGCTGCTGCTCTTCGCAAGCTGACAGGCTGTGAAATATGGAAGGCGCT
TCACCATCAAGATAACACAACTCAAGAAATAGCCCGCTTTAAAGATAATGTTGACGGCTTTGTGATTGATTCATCTGTAA
AAGGGTCTAGAGGCGGAACTGGTGTTGCATTTTCTTGGGAATGTGTGCCGGAATATCAGCAGGCGGCTATTGGTAAACGC
TGCTTTATCGCTGGCGGCGTGAATCCGGATAGCATCACACGCCTATTGAAATGGCAGCCAGAAGGAATTGACCTTGCCAG
CGGAATTGAAAAAAACGGACAAAAAGATCAGAATCTGATGAGGCTTTTAGAAGAAAGGATGAACCGATATGTATCCATAT
CCGAATGA

Upstream 100 bases:

>100_bases
GTCAATGAACATGGGGCGCGAGCTGTACTTATCGGTGAATCATTGATGAGACAAACTTCTCAGCGTAAAGCAATCCATGC
TTTGTTTAGGGAGTGAGGTT

Downstream 100 bases:

>100_bases
AATAGGCAGATACGGTGATTTTGGCGGAAAGTTTGTTCCGGAAACACTCATGCAGCCGTTAGATGAAATACAAACAGCAT
TTAAACAAATCAAGGATGAT

Product: N-(5'-phosphoribosyl)anthranilate isomerase

Products: NA

Alternate protein names: PRAI

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MKKPALKYCGIRSLKDLQLAAESQADYLGFIFAESKRKVSPEDVKKWLNQVRVEKQVAGVFVNESIETMSRIAKSLKLDV
IQLHGDEKPADAAALRKLTGCEIWKALHHQDNTTQEIARFKDNVDGFVIDSSVKGSRGGTGVAFSWECVPEYQQAAIGKR
CFIAGGVNPDSITRLLKWQPEGIDLASGIEKNGQKDQNLMRLLEERMNRYVSISE

Sequences:

>Translated_215_residues
MKKPALKYCGIRSLKDLQLAAESQADYLGFIFAESKRKVSPEDVKKWLNQVRVEKQVAGVFVNESIETMSRIAKSLKLDV
IQLHGDEKPADAAALRKLTGCEIWKALHHQDNTTQEIARFKDNVDGFVIDSSVKGSRGGTGVAFSWECVPEYQQAAIGKR
CFIAGGVNPDSITRLLKWQPEGIDLASGIEKNGQKDQNLMRLLEERMNRYVSISE
>Mature_215_residues
MKKPALKYCGIRSLKDLQLAAESQADYLGFIFAESKRKVSPEDVKKWLNQVRVEKQVAGVFVNESIETMSRIAKSLKLDV
IQLHGDEKPADAAALRKLTGCEIWKALHHQDNTTQEIARFKDNVDGFVIDSSVKGSRGGTGVAFSWECVPEYQQAAIGKR
CFIAGGVNPDSITRLLKWQPEGIDLASGIEKNGQKDQNLMRLLEERMNRYVSISE

Specific function: Bifunctional Enzyme That Catalyzes Two Sequential Steps Of Tryptophan Biosynthetic Pathway. The First Reaction Is Catalyzed By The Isomerase, Coded By The Trpf Domain; The Second Reaction Is Catalyzed By The Synthase, Coded By The Trpc Domain. [C]

COG id: COG0135

COG function: function code E; Phosphoribosylanthranilate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpF family

Homologues:

Organism=Escherichia coli, GI87081863, Length=208, Percent_Identity=29.8076923076923, Blast_Score=67, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6320210, Length=209, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRPF_BACSU (P20167)

Other databases:

- EMBL:   M80245
- EMBL:   K01391
- EMBL:   AL009126
- PIR:   D22794
- RefSeq:   NP_390146.3
- ProteinModelPortal:   P20167
- SMR:   P20167
- EnsemblBacteria:   EBBACT00000003272
- GeneID:   939006
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU22650
- GenoList:   BSU22650
- GeneTree:   EBGT00050000002322
- HOGENOM:   HBG554803
- PhylomeDB:   P20167
- ProtClustDB:   PRK01222
- BioCyc:   BSUB:BSU22650-MONOMER
- BRENDA:   5.3.1.24
- HAMAP:   MF_00135_B
- InterPro:   IPR013785
- InterPro:   IPR001240
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF00697 PRAI; SSF51366 RibP_bind_barrel

EC number: =5.3.1.24

Molecular weight: Translated: 24041; Mature: 24041

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPALKYCGIRSLKDLQLAAESQADYLGFIFAESKRKVSPEDVKKWLNQVRVEKQVAGV
CCCCCCHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
FVNESIETMSRIAKSLKLDVIQLHGDEKPADAAALRKLTGCEIWKALHHQDNTTQEIARF
HHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
KDNVDGFVIDSSVKGSRGGTGVAFSWECVPEYQQAAIGKRCFIAGGVNPDSITRLLKWQP
HCCCCCEEEECCCCCCCCCCCEEEEEECCCHHHHHHCCCEEEEECCCCHHHHHHHHHCCC
EGIDLASGIEKNGQKDQNLMRLLEERMNRYVSISE
CCCCHHHCHHCCCCCHHHHHHHHHHHHHHCEECCC
>Mature Secondary Structure
MKKPALKYCGIRSLKDLQLAAESQADYLGFIFAESKRKVSPEDVKKWLNQVRVEKQVAGV
CCCCCCHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
FVNESIETMSRIAKSLKLDVIQLHGDEKPADAAALRKLTGCEIWKALHHQDNTTQEIARF
HHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
KDNVDGFVIDSSVKGSRGGTGVAFSWECVPEYQQAAIGKRCFIAGGVNPDSITRLLKWQP
HCCCCCEEEECCCCCCCCCCCEEEEEECCCHHHHHHCCCEEEEECCCCHHHHHHHHHCCC
EGIDLASGIEKNGQKDQNLMRLLEERMNRYVSISE
CCCCHHHCHHCCCCCHHHHHHHHHHHHHHCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3924737; 9384377; 10568751