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The map label for this gene is miaA

Identifier: 255767406

GI number: 255767406

Start: 1866389

End: 1867333

Strand: Direct

Name: miaA

Synonym: BSU17330

Alternate gene names: 255767406

Gene position: 1866389-1867333 (Clockwise)

Preceding gene: 255767405

Following gene: 16078797

Centisome position: 44.27

GC content: 43.81

Gene sequence:

>945_bases
TTGAATAATACGAAGCAGCCCGTTGTCATTTTAGTCGGACCGACGGCAGTGGGGAAAACCAATTTAAGTATTCAGCTAGC
CAAATCCTTAAACGCGGAAATTATCAGCGGAGATTCGATGCAGATTTATAAAGGGATGGATATTGGAACAGCTAAAATTA
CCGAACAGGAGATGGAGGGAGTGCCCCATCATCTGATTGACATTTTAGATCCCCAAGACTCTTTCTCTACTGCCGATTAT
CAAAGCTTAGTAAGAAATAAAATCAGCGAGATTGCAAATAGAGGAAAGCTTCCGATGATTGTCGGCGGTACAGGGCTTTA
TATACAATCTGTGCTTTACGATTATACATTTACGGAAGAGGCAAATGATCCCGTGTTTCGAGAGAGCATGCAAATGGCTG
CTGAGCGGGAAGGCGCTGACTTTCTTCATGCCAAACTTGCTGCAGCAGATCCCGAGGCAGCAGCTGCGATTCATCCGAAT
AATACAAGAAGAGTCATTCGCGCACTGGAAATTTTACATACGTCCGGAAAAACGATGTCCCAGCATTTGAAGGAACAAAA
ACGAGAACTTCTGTACAATGCAGTGTTAATTGGCCTGACAATGGATAGAGACACGCTTTACGAAAGAATTAATCAGCGGG
TCGATTTGATGATGCAGTCAGGCCTTCTTCCGGAAGTGAAACGCTTATACGACAAGAACGTGAGAGACTGTCAATCAATA
CAGGCGATAGGCTATAAAGAGCTGTATGCATATTTTGACGGTTTTGTGACACTTTCCGATGCTGTCGAACAGCTAAAGCA
GAACTCGAGGCGGTATGCGAAACGCCAGCTGACGTGGTTTCGCAACAAAATGCAGGTCACATGGTTCGATATGACACCGC
CTGTTGATATGGAGCTGAAAAAAAAGGAAATTTTCACACATATAGCAGGAAAACTCGAACTTTAA

Upstream 100 bases:

>100_bases
GAAGGATGTTTACCGATGCAAAAAAAGGGCAAAATGGATAGGTGGTTGTCCATGTTGAATGCTATAATGGGGGGAGATTT
ATAAAAGAGAGTGATACATA

Downstream 100 bases:

>100_bases
TCGAAACTGTATGATATAGAGAATCAAGGAGGACGAAACATGAAACCGATTAATATTCAGGATCAGTTTTTGAATCAAAT
CCGGAAAGAAAATACGTATG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MNNTKQPVVILVGPTAVGKTNLSIQLAKSLNAEIISGDSMQIYKGMDIGTAKITEQEMEGVPHHLIDILDPQDSFSTADY
QSLVRNKISEIANRGKLPMIVGGTGLYIQSVLYDYTFTEEANDPVFRESMQMAAEREGADFLHAKLAAADPEAAAAIHPN
NTRRVIRALEILHTSGKTMSQHLKEQKRELLYNAVLIGLTMDRDTLYERINQRVDLMMQSGLLPEVKRLYDKNVRDCQSI
QAIGYKELYAYFDGFVTLSDAVEQLKQNSRRYAKRQLTWFRNKMQVTWFDMTPPVDMELKKKEIFTHIAGKLEL

Sequences:

>Translated_314_residues
MNNTKQPVVILVGPTAVGKTNLSIQLAKSLNAEIISGDSMQIYKGMDIGTAKITEQEMEGVPHHLIDILDPQDSFSTADY
QSLVRNKISEIANRGKLPMIVGGTGLYIQSVLYDYTFTEEANDPVFRESMQMAAEREGADFLHAKLAAADPEAAAAIHPN
NTRRVIRALEILHTSGKTMSQHLKEQKRELLYNAVLIGLTMDRDTLYERINQRVDLMMQSGLLPEVKRLYDKNVRDCQSI
QAIGYKELYAYFDGFVTLSDAVEQLKQNSRRYAKRQLTWFRNKMQVTWFDMTPPVDMELKKKEIFTHIAGKLEL
>Mature_314_residues
MNNTKQPVVILVGPTAVGKTNLSIQLAKSLNAEIISGDSMQIYKGMDIGTAKITEQEMEGVPHHLIDILDPQDSFSTADY
QSLVRNKISEIANRGKLPMIVGGTGLYIQSVLYDYTFTEEANDPVFRESMQMAAEREGADFLHAKLAAADPEAAAAIHPN
NTRRVIRALEILHTSGKTMSQHLKEQKRELLYNAVLIGLTMDRDTLYERINQRVDLMMQSGLLPEVKRLYDKNVRDCQSI
QAIGYKELYAYFDGFVTLSDAVEQLKQNSRRYAKRQLTWFRNKMQVTWFDMTPPVDMELKKKEIFTHIAGKLEL

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=323, Percent_Identity=33.4365325077399, Blast_Score=180, Evalue=2e-45,
Organism=Escherichia coli, GI1790613, Length=306, Percent_Identity=37.2549019607843, Blast_Score=208, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI25144712, Length=301, Percent_Identity=31.5614617940199, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6324848, Length=292, Percent_Identity=35.2739726027397, Blast_Score=198, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24649830, Length=336, Percent_Identity=32.7380952380952, Blast_Score=182, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_BACSU (O31795)

Other databases:

- EMBL:   AL009126
- PIR:   G69657
- RefSeq:   NP_389615.2
- ProteinModelPortal:   O31795
- SMR:   O31795
- EnsemblBacteria:   EBBACT00000001623
- GeneID:   940067
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU17330
- NMPDR:   fig|224308.1.peg.1737
- GenoList:   BSU17330
- GeneTree:   EBGT00050000001497
- HOGENOM:   HBG559459
- ProtClustDB:   PRK00091
- BioCyc:   BSUB:BSU17330-MONOMER
- BRENDA:   2.5.1.8
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35633; Mature: 35633

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNTKQPVVILVGPTAVGKTNLSIQLAKSLNAEIISGDSMQIYKGMDIGTAKITEQEMEG
CCCCCCCEEEEECCCCCCCCCCEEEECCCCCCEEECCCCEEEEECCCCCHHHCCHHHHHC
VPHHLIDILDPQDSFSTADYQSLVRNKISEIANRGKLPMIVGGTGLYIQSVLYDYTFTEE
CCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHCEECCC
ANDPVFRESMQMAAEREGADFLHAKLAAADPEAAAAIHPNNTRRVIRALEILHTSGKTMS
CCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHCCCHHHH
QHLKEQKRELLYNAVLIGLTMDRDTLYERINQRVDLMMQSGLLPEVKRLYDKNVRDCQSI
HHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHH
QAIGYKELYAYFDGFVTLSDAVEQLKQNSRRYAKRQLTWFRNKMQVTWFDMTPPVDMELK
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHH
KKEIFTHIAGKLEL
HHHHHHHHHCCCCC
>Mature Secondary Structure
MNNTKQPVVILVGPTAVGKTNLSIQLAKSLNAEIISGDSMQIYKGMDIGTAKITEQEMEG
CCCCCCCEEEEECCCCCCCCCCEEEECCCCCCEEECCCCEEEEECCCCCHHHCCHHHHHC
VPHHLIDILDPQDSFSTADYQSLVRNKISEIANRGKLPMIVGGTGLYIQSVLYDYTFTEE
CCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHCEECCC
ANDPVFRESMQMAAEREGADFLHAKLAAADPEAAAAIHPNNTRRVIRALEILHTSGKTMS
CCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHCCCHHHH
QHLKEQKRELLYNAVLIGLTMDRDTLYERINQRVDLMMQSGLLPEVKRLYDKNVRDCQSI
HHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHH
QAIGYKELYAYFDGFVTLSDAVEQLKQNSRRYAKRQLTWFRNKMQVTWFDMTPPVDMELK
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHH
KKEIFTHIAGKLEL
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377