Click here to switch to the map view.

The map label for this gene is pksF

Identifier: 255767394

GI number: 255767394

Start: 1788695

End: 1789942

Strand: Direct

Name: pksF

Synonym: BSU17140

Alternate gene names: 255767394

Gene position: 1788695-1789942 (Clockwise)

Preceding gene: 18677776

Following gene: 50812233

Centisome position: 42.43

GC content: 46.71

Gene sequence:

>1248_bases
ATGACAAAGTGCAATCTGCCTGAGGTGGTGGTGACCGGTGTAGGCGTTACGGCCTCTATCGGTCAAGGGAAAGAGGACTT
TGCTTCCTCGCTGCTGTCCGGCAGACATGCCTTTGATGTCATGAAGCGTTCAGGCAGACAAAAAGACAGCCGGTTTATCG
GAGCCGAAATTGCGTCATTGTCTTATCCTGACCGACTCTCTAAAAAAATGTTGCGAAAAGCATCATTCTCCAGCCGCGCT
GCATTGGTAACTCTCACAGAGGCTTGGGAGGAGGCTGAATTGGACGATGCAGATTCCTCACGTATCGGACTGGTTGTTGG
CGGATCAAATTTTCAACAGAGAGAAAACTTTGAAGTTTATGAAAGGTATCAAGACCGCTCCGGTTTTATTTCTCCGGCGT
ATGGTCTGTCTTTCATGGACAGCGATCTTTGCGGGATATGTACAGATCAGTTTGGCATCACGGGATTAGCATATACGGTG
GGAGGAGCTTCGGCCAGCGGCCAGTTAGCAGTCATTCATGCGATTCAGCAAGTCCTGTCAGGCGAAGTTGATACTTGCAT
AGCACTTGGTGCTTTAATGGATCTTTCTTATATGGAATGCGAGGCGCTAAGGGCTTTAGGCGCTATGGGGACGGACAAAT
ATGCTGATGAACCTGAAAATGCGTGCCGTCCCTTCGATCAAAACAGAGACGGGTTTATCTATGGAGAATCGTGCGGTGCA
TTGGTAATAGAACGAAAGGAGACGGCTTTACGAAGAGGTCTAAAACCTTATGCAGCATTGTCTGGCTGGTCAATCAAACT
GGACGGAAACCGGAATCCTGATCCTTCATTAGAAGGAGAGATTCATGTCATCCAGAAGGCACTGGAGCGTGCCAGGCTTT
TACCAGAAGATATTGATTATATTAATCCTCATGGAACAGGCTCTTTTATTGGAGATGAAATAGAATTAAAGGCGCTCCGT
GCATGCCGGCTTTCCCATGCTTATATCAATGCAACAAAATCGATAACCGGACACGGGCTTAGTGCGGCGGGCATCGTAGA
GATTATTTCTGTTTTGCTGCAAATGAAAAAATCTGCCCTGCATCCGAGCAGGAATTTGGATCATCCAATCGATGATTCCT
TTCATTGGGTAAATGAGAAATCGATTTCATACCGCATCAAAAATGCGCTGAGCTTAAGCATGGGGTTTGGCGGAATGAAC
ACCGCGGTTTGTATACAAAACATTGAGAAATGTGGAGGCGAATCATAA

Upstream 100 bases:

>100_bases
GCTGAAATTATCACGATGGTGCTAGAGGATTTATCGTTAAAAATCCCTCGCATTGAGCTATCCGGGGTGAAAAACATCGG
TGAATTAGCTGAGGTGCTTT

Downstream 100 bases:

>100_bases
ATGGTTTCAGCCGGAATAGAAGCGATGAATGTCTTTGGCGGAACGGCATACCTTGATGTCATGGAGCTGGCCAAATACAG
ACACTTAGATACTGCGAGAT

Product: polyketide beta-ketoacyl:acyl carrier protein synthase

Products: NA

Alternate protein names: Malonyl acyl-carrier-protein decarboxylase

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MTKCNLPEVVVTGVGVTASIGQGKEDFASSLLSGRHAFDVMKRSGRQKDSRFIGAEIASLSYPDRLSKKMLRKASFSSRA
ALVTLTEAWEEAELDDADSSRIGLVVGGSNFQQRENFEVYERYQDRSGFISPAYGLSFMDSDLCGICTDQFGITGLAYTV
GGASASGQLAVIHAIQQVLSGEVDTCIALGALMDLSYMECEALRALGAMGTDKYADEPENACRPFDQNRDGFIYGESCGA
LVIERKETALRRGLKPYAALSGWSIKLDGNRNPDPSLEGEIHVIQKALERARLLPEDIDYINPHGTGSFIGDEIELKALR
ACRLSHAYINATKSITGHGLSAAGIVEIISVLLQMKKSALHPSRNLDHPIDDSFHWVNEKSISYRIKNALSLSMGFGGMN
TAVCIQNIEKCGGES

Sequences:

>Translated_415_residues
MTKCNLPEVVVTGVGVTASIGQGKEDFASSLLSGRHAFDVMKRSGRQKDSRFIGAEIASLSYPDRLSKKMLRKASFSSRA
ALVTLTEAWEEAELDDADSSRIGLVVGGSNFQQRENFEVYERYQDRSGFISPAYGLSFMDSDLCGICTDQFGITGLAYTV
GGASASGQLAVIHAIQQVLSGEVDTCIALGALMDLSYMECEALRALGAMGTDKYADEPENACRPFDQNRDGFIYGESCGA
LVIERKETALRRGLKPYAALSGWSIKLDGNRNPDPSLEGEIHVIQKALERARLLPEDIDYINPHGTGSFIGDEIELKALR
ACRLSHAYINATKSITGHGLSAAGIVEIISVLLQMKKSALHPSRNLDHPIDDSFHWVNEKSISYRIKNALSLSMGFGGMN
TAVCIQNIEKCGGES
>Mature_414_residues
TKCNLPEVVVTGVGVTASIGQGKEDFASSLLSGRHAFDVMKRSGRQKDSRFIGAEIASLSYPDRLSKKMLRKASFSSRAA
LVTLTEAWEEAELDDADSSRIGLVVGGSNFQQRENFEVYERYQDRSGFISPAYGLSFMDSDLCGICTDQFGITGLAYTVG
GASASGQLAVIHAIQQVLSGEVDTCIALGALMDLSYMECEALRALGAMGTDKYADEPENACRPFDQNRDGFIYGESCGAL
VIERKETALRRGLKPYAALSGWSIKLDGNRNPDPSLEGEIHVIQKALERARLLPEDIDYINPHGTGSFIGDEIELKALRA
CRLSHAYINATKSITGHGLSAAGIVEIISVLLQMKKSALHPSRNLDHPIDDSFHWVNEKSISYRIKNALSLSMGFGGMNT
AVCIQNIEKCGGES

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It decarboxylates selectively the malonyl group attached on the acyl-carrier-protein AcpK (Mal-AcpK)

COG id: COG0304

COG function: function code IQ; 3-oxoacyl-(acyl-carrier-protein) synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-ketoacyl-ACP synthases family

Homologues:

Organism=Homo sapiens, GI8923559, Length=426, Percent_Identity=27.9342723004695, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI223972627, Length=144, Percent_Identity=37.5, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI41872631, Length=269, Percent_Identity=26.7657992565056, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788663, Length=417, Percent_Identity=29.9760191846523, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1787337, Length=416, Percent_Identity=28.8461538461538, Blast_Score=131, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI71983541, Length=420, Percent_Identity=26.1904761904762, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI71983535, Length=225, Percent_Identity=31.5555555555556, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI212642053, Length=270, Percent_Identity=27.7777777777778, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17550940, Length=276, Percent_Identity=27.1739130434783, Blast_Score=78, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6320904, Length=443, Percent_Identity=25.5079006772009, Blast_Score=129, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24644341, Length=413, Percent_Identity=27.8450363196126, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI221330659, Length=346, Percent_Identity=23.9884393063584, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19920632, Length=346, Percent_Identity=23.9884393063584, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24581345, Length=277, Percent_Identity=22.0216606498195, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: 24024 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 30,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PKSF_BACSU (P40804)

Other databases:

- EMBL:   U11039
- EMBL:   AL009126
- PIR:   D69678
- RefSeq:   NP_389594.2
- ProteinModelPortal:   P40804
- SMR:   P40804
- EnsemblBacteria:   EBBACT00000001944
- GeneID:   939500
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU17140
- NMPDR:   fig|224308.1.peg.1716
- GenoList:   BSU17140
- GeneTree:   EBGT00050000000384
- HOGENOM:   HBG757733
- ProtClustDB:   PRK07103
- BioCyc:   BSUB:BSU17140-MONOMER
- GO:   GO:0005737
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016039
- InterPro:   IPR016038
- Gene3D:   G3DSA:3.40.47.10

Pfam domain/function: PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C; SSF53901 Thiolase-like

EC number: =4.1.1.87

Molecular weight: Translated: 45009; Mature: 44878

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKCNLPEVVVTGVGVTASIGQGKEDFASSLLSGRHAFDVMKRSGRQKDSRFIGAEIASL
CCCCCCCCEEEECCCEEECCCCCHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHC
SYPDRLSKKMLRKASFSSRAALVTLTEAWEEAELDDADSSRIGLVVGGSNFQQRENFEVY
CCHHHHHHHHHHHHCCCCCEEEEEEHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCHHH
ERYQDRSGFISPAYGLSFMDSDLCGICTDQFGITGLAYTVGGASASGQLAVIHAIQQVLS
HHHHHCCCCCCHHCCCHHCCCCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHC
GEVDTCIALGALMDLSYMECEALRALGAMGTDKYADEPENACRPFDQNRDGFIYGESCGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCEEEECCCCE
LVIERKETALRRGLKPYAALSGWSIKLDGNRNPDPSLEGEIHVIQKALERARLLPEDIDY
EEEECHHHHHHCCCCCHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCHHHCCC
INPHGTGSFIGDEIELKALRACRLSHAYINATKSITGHGLSAAGIVEIISVLLQMKKSAL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHC
HPSRNLDHPIDDSFHWVNEKSISYRIKNALSLSMGFGGMNTAVCIQNIEKCGGES
CCCCCCCCCCCCCCEECCCCHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKCNLPEVVVTGVGVTASIGQGKEDFASSLLSGRHAFDVMKRSGRQKDSRFIGAEIASL
CCCCCCCEEEECCCEEECCCCCHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHC
SYPDRLSKKMLRKASFSSRAALVTLTEAWEEAELDDADSSRIGLVVGGSNFQQRENFEVY
CCHHHHHHHHHHHHCCCCCEEEEEEHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCHHH
ERYQDRSGFISPAYGLSFMDSDLCGICTDQFGITGLAYTVGGASASGQLAVIHAIQQVLS
HHHHHCCCCCCHHCCCHHCCCCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHC
GEVDTCIALGALMDLSYMECEALRALGAMGTDKYADEPENACRPFDQNRDGFIYGESCGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCEEEECCCCE
LVIERKETALRRGLKPYAALSGWSIKLDGNRNPDPSLEGEIHVIQKALERARLLPEDIDY
EEEECHHHHHHCCCCCHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCHHHCCC
INPHGTGSFIGDEIELKALRACRLSHAYINATKSITGHGLSAAGIVEIISVLLQMKKSAL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHC
HPSRNLDHPIDDSFHWVNEKSISYRIKNALSLSMGFGGMNTAVCIQNIEKCGGES
CCCCCCCCCCCCCCEECCCCHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377