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The map label for this gene is pyrP

Identifier: 255767361

GI number: 255767361

Start: 1619023

End: 1620330

Strand: Direct

Name: pyrP

Synonym: BSU15480

Alternate gene names: 255767361

Gene position: 1619023-1620330 (Clockwise)

Preceding gene: 16078611

Following gene: 255767362

Centisome position: 38.41

GC content: 49.46

Gene sequence:

>1308_bases
ATGAGTAAGAAAAAAGTAAATTTAGGGGTCAGGGATGTCCCGACACCTTTCTCTTGGGTTTCATTCAGCCTTCAGCATTT
GTTTGCCATGTTTGGCTCAACCATTTTGGTTCCGAAGCTCGTCGGAATGAGTCCTGCTGTGGCGTTGGTGACAAGCGGCA
TCGGAACACTGGCGTACCTTCTTATTACCAAAGGACAAATTCCGGCGTATCTCGGTTCATCCTTCGCCTTTATTTCTCCG
ATCATTCTGGTAAAAGCGACCGGCGGGCCGGGAGCGGCAATGGTTGGAGCGTTTCTTGCAGGGCTGGTGTACGGGCTGAT
TGCCTTATTGATTAGGCAGCTTGGAACAGGATGGCTGATGAAGATTCTCCCGCCTGTAGTCGTAGGGCCTGTTATTATCG
TCATCGGGCTGGGACTGGCAAGCACTGCAGTAAACATGGCGATGTATGCTGATCCGAACGCGAGTGAGTTGGTCTACAGC
TTAAAGCACTTTAGTGTCGCAGGAGTTACGCTGGCAATTACGATTATTTGTGCGATTTTCTTACGAGGGTTTTTAAGCCT
GATTCCGGTTCTGATCGGAATCATCGGCGGATACCTGTTTGCCCTTACTCAAGGGATTGTCAACTTCCAGCCGGTGCTTG
ACGCGAAATGGTTTGCAGTGCCTGAATTTATCATTCCGTTCAAAGATTATTCACCGTCAGTTACGCTCGGCATCGCAGCC
GCAATGGTTCCTGTCGCATTTGTCACAATGTCAGAGCATATCGGCCACCAAATGGTGCTGAGCAAGGTTGTCGGACAAGA
CTTCATTAAAAAGCCAGGTCTTCATCGCTCTATTATGGGTGACAGTGTGGCGACAATCCTCGCTTCCCTGATCGGCGGCC
CTCCGACAACGACTTACGGAGAAAACATTGGCGTGCTGGCCATCACAAGAGTATTCAGCGTCTTTGTCATCGGGGGCGCG
GCAGTGATTGCCCTTTGCTTCGGCTTTATCGGCAAAATTTCAGCGCTGATCAGTTCAGTGCCGTCAGCGGTCATGGGAGG
CGTCTCCTTCCTGCTGTTCGGAATCATTGCTTCAAGCGGCCTGAGAATGCTGATTGACAACAAAATTGATTATGAAAACA
ACAGAAACCTCATTATTACATCAGTTATCCTTGTCATCGGTGTAGGAGGCGCTTTTATCCAAGTGTCTCAGGGCGGATTC
CAAGTGTCAGGAATGGCGCTTGCCGCAATTGTCGGTGTCATCTTAAACCTGATTCTTCCGCAGGCGAAGGAAGAGCAGGC
AGACACATCTGAACAACATCATATTTAA

Upstream 100 bases:

>100_bases
AGGTCTTTGTATGCCTCTTTGCGTAAAAAAGCAAAGAGGTTTTTTTATACAGTCATTGAGTCATCCTAAAATGAAAGTCA
ACAATCAGGGGGAAATCATC

Downstream 100 bases:

>100_bases
AACCTTTTAATGAAAGTCCAGAGAGGCTTGGAAGGGTTATGAAGAGAAGGAAGCTTCAATGCTGCCCTCTATTTAACCAT
ACCCCGAGTCTATCTTAGAC

Product: uracil permease

Products: Proton [Cytoplasm]; uracil [Cytoplasm] [C]

Alternate protein names: Uracil transporter

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSKKKVNLGVRDVPTPFSWVSFSLQHLFAMFGSTILVPKLVGMSPAVALVTSGIGTLAYLLITKGQIPAYLGSSFAFISP
IILVKATGGPGAAMVGAFLAGLVYGLIALLIRQLGTGWLMKILPPVVVGPVIIVIGLGLASTAVNMAMYADPNASELVYS
LKHFSVAGVTLAITIICAIFLRGFLSLIPVLIGIIGGYLFALTQGIVNFQPVLDAKWFAVPEFIIPFKDYSPSVTLGIAA
AMVPVAFVTMSEHIGHQMVLSKVVGQDFIKKPGLHRSIMGDSVATILASLIGGPPTTTYGENIGVLAITRVFSVFVIGGA
AVIALCFGFIGKISALISSVPSAVMGGVSFLLFGIIASSGLRMLIDNKIDYENNRNLIITSVILVIGVGGAFIQVSQGGF
QVSGMALAAIVGVILNLILPQAKEEQADTSEQHHI

Sequences:

>Translated_435_residues
MSKKKVNLGVRDVPTPFSWVSFSLQHLFAMFGSTILVPKLVGMSPAVALVTSGIGTLAYLLITKGQIPAYLGSSFAFISP
IILVKATGGPGAAMVGAFLAGLVYGLIALLIRQLGTGWLMKILPPVVVGPVIIVIGLGLASTAVNMAMYADPNASELVYS
LKHFSVAGVTLAITIICAIFLRGFLSLIPVLIGIIGGYLFALTQGIVNFQPVLDAKWFAVPEFIIPFKDYSPSVTLGIAA
AMVPVAFVTMSEHIGHQMVLSKVVGQDFIKKPGLHRSIMGDSVATILASLIGGPPTTTYGENIGVLAITRVFSVFVIGGA
AVIALCFGFIGKISALISSVPSAVMGGVSFLLFGIIASSGLRMLIDNKIDYENNRNLIITSVILVIGVGGAFIQVSQGGF
QVSGMALAAIVGVILNLILPQAKEEQADTSEQHHI
>Mature_434_residues
SKKKVNLGVRDVPTPFSWVSFSLQHLFAMFGSTILVPKLVGMSPAVALVTSGIGTLAYLLITKGQIPAYLGSSFAFISPI
ILVKATGGPGAAMVGAFLAGLVYGLIALLIRQLGTGWLMKILPPVVVGPVIIVIGLGLASTAVNMAMYADPNASELVYSL
KHFSVAGVTLAITIICAIFLRGFLSLIPVLIGIIGGYLFALTQGIVNFQPVLDAKWFAVPEFIIPFKDYSPSVTLGIAAA
MVPVAFVTMSEHIGHQMVLSKVVGQDFIKKPGLHRSIMGDSVATILASLIGGPPTTTYGENIGVLAITRVFSVFVIGGAA
VIALCFGFIGKISALISSVPSAVMGGVSFLLFGIIASSGLRMLIDNKIDYENNRNLIITSVILVIGVGGAFIQVSQGGFQ
VSGMALAAIVGVILNLILPQAKEEQADTSEQHHI

Specific function: Transport of uracil in the cell

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily

Homologues:

Organism=Homo sapiens, GI44680143, Length=448, Percent_Identity=24.1071428571429, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI44680145, Length=445, Percent_Identity=24.0449438202247, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI44680148, Length=445, Percent_Identity=23.8202247191011, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI40316845, Length=445, Percent_Identity=23.8202247191011, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788843, Length=447, Percent_Identity=42.9530201342282, Blast_Score=351, Evalue=5e-98,
Organism=Escherichia coli, GI87081818, Length=428, Percent_Identity=35.5140186915888, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI87082181, Length=451, Percent_Identity=27.7161862527716, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1790087, Length=441, Percent_Identity=24.2630385487528, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082178, Length=322, Percent_Identity=24.223602484472, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17541904, Length=301, Percent_Identity=26.9102990033223, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17542262, Length=431, Percent_Identity=25.754060324826, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21356175, Length=508, Percent_Identity=23.8188976377953, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRP_BACSU (P39766)

Other databases:

- EMBL:   M59757
- EMBL:   AL009126
- PIR:   A38984
- RefSeq:   NP_389431.2
- EnsemblBacteria:   EBBACT00000002505
- GeneID:   936604
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU15480
- NMPDR:   fig|224308.1.peg.1550
- GenoList:   BSU15480
- GeneTree:   EBGT00050000001001
- HOGENOM:   HBG470187
- ProtClustDB:   CLSK887278
- BioCyc:   BSUB:BSU15480-MONOMER
- InterPro:   IPR006042
- InterPro:   IPR006043
- TIGRFAMs:   TIGR00801

Pfam domain/function: PF00860 Xan_ur_permease

EC number: NA

Molecular weight: Translated: 45453; Mature: 45322

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xee64a88)-; HASH(0xbacf750)-; HASH(0xec4f7bc)-; HASH(0xedffd04)-; HASH(0xf14c790)-; HASH(0xf04f098)-; HASH(0xf0afeb0)-; HASH(0xf015d98)-; HASH(0xb6a1dbc)-; HASH(0xec4d9b4)-; HASH(0xb8a6e20)-; HASH(0xe22da68)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCHHHHCCCCCCCCH
>Mature Secondary Structure 
SKKKVNLGVRDVPTPFSWVSFSLQHLFAMFGSTILVPKLVGMSPAVALVTSGIGTLAYL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHH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CCHHHHCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; uracil [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uracil [Periplasm] = Proton [Cytoplasm] + uracil [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1709162; 8206849; 9384377