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The map label for this gene is sigE

Identifier: 255767354

GI number: 255767354

Start: 1604771

End: 1605490

Strand: Direct

Name: sigE

Synonym: BSU15320

Alternate gene names: 255767354

Gene position: 1604771-1605490 (Clockwise)

Preceding gene: 16078595

Following gene: 16078597

Centisome position: 38.07

GC content: 40.56

Gene sequence:

>720_bases
ATGAAAAAACTGAAATTACGGTTGACGCACCTCTGGTATAAGCTGCTGATGAAACTTGGGCTGAAAAGTGATGAAGTCTA
TTACATAGGCGGGAGTGAAGCCCTGCCGCCTCCATTATCTAAAGATGAGGAGCAGGTTTTGTTAATGAAGCTCCCAAACG
GCGATCAGGCGGCGCGCGCCATTCTAATTGAACGCAATTTGCGTCTGGTCGTATATATCGCCCGTAAATTTGAAAATACG
GGAATTAATATAGAGGATTTAATCAGCATCGGTACCATCGGTCTAATCAAAGCTGTTAATACATTTAATCCAGAAAAGAA
AATCAAGCTTGCTACCTATGCCTCCCGGTGTATAGAAAATGAAATCCTGATGTATTTAAGAAGAAATAACAAAATCCGTT
CAGAGGTTTCCTTTGATGAACCGCTTAATATTGATTGGGACGGCAATGAGCTTTTGCTTTCTGATGTGCTCGGCACTGAC
GATGACATCATCACTAAAGACATAGAAGCTAACGTCGATAAAAAGCTTTTGAAAAAAGCGCTTGAACAGCTTAATGAGAG
AGAAAAGCAAATCATGGAGCTGCGGTTTGGGCTTGTCGGTGAAGAAGAAAAAACCCAAAAGGATGTAGCGGATATGATGG
GGATTTCTCAGTCTTATATTTCGCGGCTTGAGAAAAGAATTATAAAAAGGTTGAGAAAAGAGTTCAACAAAATGGTGTAA

Upstream 100 bases:

>100_bases
AAATGCTTTCGGGCAAGGCTGTCAAACACGTTTCATAATGTTCGCAAATGTCCGTTACTTATCATACTCAAACGGCGTGA
CATTTAGAAGGGAGAGGAAG

Downstream 100 bases:

>100_bases
AAAATTTTATGGTTAGAACCCCTTGATTTTACAGGGATTTCCTGATTTCGACAGTTTTTCGGTCTGAGTGCAGTGCATAT
TTTTCCCACCCAAGGAGATA

Product: sporulation sigma factor SigE

Products: NA

Alternate protein names: P31; Sigma-29; Stage II sporulation protein GB

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MKKLKLRLTHLWYKLLMKLGLKSDEVYYIGGSEALPPPLSKDEEQVLLMKLPNGDQAARAILIERNLRLVVYIARKFENT
GINIEDLISIGTIGLIKAVNTFNPEKKIKLATYASRCIENEILMYLRRNNKIRSEVSFDEPLNIDWDGNELLLSDVLGTD
DDIITKDIEANVDKKLLKKALEQLNEREKQIMELRFGLVGEEEKTQKDVADMMGISQSYISRLEKRIIKRLRKEFNKMV

Sequences:

>Translated_239_residues
MKKLKLRLTHLWYKLLMKLGLKSDEVYYIGGSEALPPPLSKDEEQVLLMKLPNGDQAARAILIERNLRLVVYIARKFENT
GINIEDLISIGTIGLIKAVNTFNPEKKIKLATYASRCIENEILMYLRRNNKIRSEVSFDEPLNIDWDGNELLLSDVLGTD
DDIITKDIEANVDKKLLKKALEQLNEREKQIMELRFGLVGEEEKTQKDVADMMGISQSYISRLEKRIIKRLRKEFNKMV
>Mature_239_residues
MKKLKLRLTHLWYKLLMKLGLKSDEVYYIGGSEALPPPLSKDEEQVLLMKLPNGDQAARAILIERNLRLVVYIARKFENT
GINIEDLISIGTIGLIKAVNTFNPEKKIKLATYASRCIENEILMYLRRNNKIRSEVSFDEPLNIDWDGNELLLSDVLGTD
DDIITKDIEANVDKKLLKKALEQLNEREKQIMELRFGLVGEEEKTQKDVADMMGISQSYISRLEKRIIKRLRKEFNKMV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family

Homologues:

Organism=Escherichia coli, GI1789098, Length=256, Percent_Identity=29.296875, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1789871, Length=106, Percent_Identity=45.2830188679245, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): RPSE_BACSU (P06222)

Other databases:

- EMBL:   X01180
- EMBL:   M57606
- EMBL:   AL009126
- EMBL:   M15804
- EMBL:   X17344
- PIR:   S07337
- RefSeq:   NP_389415.2
- ProteinModelPortal:   P06222
- SMR:   P06222
- EnsemblBacteria:   EBBACT00000000229
- GeneID:   939683
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU15320
- GenoList:   BSU15320
- GeneTree:   EBGT00070000031774
- HOGENOM:   HBG745096
- ProtClustDB:   PRK08301
- BioCyc:   BSUB:BSU15320-MONOMER
- InterPro:   IPR014284
- InterPro:   IPR014200
- InterPro:   IPR016263
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PIRSF:   PIRSF000770
- PRINTS:   PR00046
- TIGRFAMs:   TIGR02937
- TIGRFAMs:   TIGR02835

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4; SSF88946 Sigma_r2; SSF88659 Sigma_r3_r4

EC number: NA

Molecular weight: Translated: 27653; Mature: 27653

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS00715 SIGMA70_1; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLKLRLTHLWYKLLMKLGLKSDEVYYIGGSEALPPPLSKDEEQVLLMKLPNGDQAARA
CCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCEEEEECCCCCHHHHH
ILIERNLRLVVYIARKFENTGINIEDLISIGTIGLIKAVNTFNPEKKIKLATYASRCIEN
HHHCCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EILMYLRRNNKIRSEVSFDEPLNIDWDGNELLLSDVLGTDDDIITKDIEANVDKKLLKKA
HHHHHHHHCCCHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHH
LEQLNEREKQIMELRFGLVGEEEKTQKDVADMMGISQSYISRLEKRIIKRLRKEFNKMV
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKLKLRLTHLWYKLLMKLGLKSDEVYYIGGSEALPPPLSKDEEQVLLMKLPNGDQAARA
CCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCEEEEECCCCCHHHHH
ILIERNLRLVVYIARKFENTGINIEDLISIGTIGLIKAVNTFNPEKKIKLATYASRCIEN
HHHCCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EILMYLRRNNKIRSEVSFDEPLNIDWDGNELLLSDVLGTDDDIITKDIEANVDKKLLKKA
HHHHHHHHCCCHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHH
LEQLNEREKQIMELRFGLVGEEEKTQKDVADMMGISQSYISRLEKRIIKRLRKEFNKMV
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6438529; 9384377; 3104904; 2106671