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The map label for this gene is pbpB

Identifier: 255767346

GI number: 255767346

Start: 1581947

End: 1584097

Strand: Direct

Name: pbpB

Synonym: BSU15160

Alternate gene names: 255767346

Gene position: 1581947-1584097 (Clockwise)

Preceding gene: 16078579

Following gene: 255767347

Centisome position: 37.53

GC content: 42.12

Gene sequence:

>2151_bases
ATGATTCAAATGCCAAAAAAGAATAAATTTATGAATAGAGGAGCAGCGATTCTAAGTATTTGTTTCGCTCTCTTTTTCTT
TGTCATCCTGGGGAGAATGGCATATATTCAAATAACCGGAAAAGCGAACGGCGAAGTGCTTGCGACAAAAGCGACAGAGC
AGCATGAAAAGAAACGGACCATCGAAGCGAGCCGCGGCTCGATTTTAGACCGAAAAGGAAAAGTCATTGCAGAGGACACA
GCGACGTATAAACTGATTGCGATTCTCGATAAAAAAATGACCACTGATGTCAAGCATCCTCAGCATGTTGTAAACAAAGA
AAAAACGGCGGAAGCATTGTCTAAAGTGATAAACTTGGACAAGGCCGACATCCTTGATATCTTAAATAAAGATGCAAAAC
AAGTCGAGTTTGGCTCAGCTGGCCGGGATATTACGTATTCACAAAAGCAAAAAATCGAAAAAATGAAACTCCCGGGCATT
TCATTTTTACGGGATACAAAACGCTACTATCCAAACGGAGTATTTGCATCTAATCTAATCGGCTATGCCGAGGTTGATGA
AGAAACAAATGAAATTTCCGGCGCGATGGGATTAGAAAAAGTGCTGGATAAGTATTTGAAGGAGCGGGACGGATATGTGA
CATATGAAAGCGACAAATCCGGCTGGGAGCTCCCGAACAGCAAAAATAAAATTACAGCGCCAAAAAATGGTGACAATGTA
TATTTAACCATTGACCAGAAAATTCAAACCTTTTTGGAAGACAGCATGACAAAAGTGGCGCAAAAGTATAATCCGAAAAA
AATCATGGCCGCAGTCGTTGATCCGAAAACGGGCAAAGTCCTTGCCATGGGACAGCGCCCAAGCTTTGATCCGAACAAGC
GCGATGTGACAAACTACTATAATGATTTGATTTCATATGCGTATGAACCCGGGTCCACGATGAAGATCTTTACACTCGCT
GCTGCGATGCAGGAAAACGTGTTTAATGCGAACGAAAAATATAAATCAGGGACATTTGAAGTTGGCGGAGCACCTGTTAA
GGATCACAATAACGGTGTAGGCTGGGGTCCGACAACATATCATGACGGCGTCCTAAGGTCGTCGAATGTCGCTTTTGCGA
AACTGGCGAAAGAAAAGCTGGGATATGACCGCCTCAATCAATATTTGCACAAATTCAATTTTTACCAGAAAACAGGTATT
GATTTGCCAGGAGAAGTCTCCAGCAAAATCAACTTTAAATATGAATTTGATAAGGCGTCAACTGCATATGGCCAAGCGTC
TGCGGTCACACCAATTCAGCAGATTCAGGCCGCAACGGCAATTGCAAATGACGGTAAAATGATGAAACCTTATGTCATTG
ACCATATCGTTGACCCTGATAAAGATAAAACTATTTATCAAAATAAGCCGGAATCAGCCGGAACTCCAATTTCTGCAAGT
ACTGCGAAAAAGGTGCGGGATATATTAGGAGAAGTCGTTACATCGAAGATCGGAACAGGGCAAGCTTATAAAATCGAAGG
TTTTGATGTTGCCGGGAAAACAGGTACTGCGCAAATAGCCGGTAAAGGCGGATATTTAGACGGCACAGATAACTATATCT
TTTCGTTTATGGGTATGGCGCCAAAAGATGATCCTGAGCTTTTAATTTATGTTGCTGTACAGCAGCCGCAACTAAAAGCA
GGACAAAGCAGTTCAGATCCAGTATCAGAAATCTTCAATCCAACAATGAAAAATAGCCTGCACTACCTAAACATCGAACC
AACTGAAAAATCTGACTCAGATAAGGAAGAAACAAAAGCGCAGACAATGCCTGATTTAACAGATCAAACGGTAGCTGCTG
CTCAAAAGAAAGCAAAAGAGGAGAATCTCACACCAATTGTCATCGGCAGTGATGTCGCTGTAAAAGAACAGTATCCGAAA
GCGGATGAGGAAGTTCTCACCAATCAAAAGGTCTTCCTGAAAACGGGCGGCAAAATCAAAATGCCTGATATGACAGGCTG
GTCGAGAAGAGAGGTTCTGCAGTACGGCGAGCTCGCGGGAATTCATATTGAAGTAAGCGGACAAGGCTACGCTGTCAGCC
AAAGTGTTAAGAAAGACAAAGAAATCAAAGACAAAACCGTAATCAAGGTTAAGTTTAAAAATCCTGATTAA

Upstream 100 bases:

>100_bases
AAAAAAGCGTTGCTGATTTAAGCAAACCGCAGCGCATTATGGACATTGCGAAAAAGAACGGCTTGAATTTAAAAGATAAA
AAAGTGAAAAACATACAGGA

Downstream 100 bases:

>100_bases
AAAGAAAGCCGTCGTTATGCAGGCTTTCTTTTTTTATGCCTTCAGAGGAGCATCGTTCTACCTGTCCAAATTCAGGCATA
AAATGAAACAAGCCTAAATA

Product: penicillin-binding protein 2B

Products: NA

Alternate protein names: PBP-2B; Penicillin-binding protein B

Number of amino acids: Translated: 716; Mature: 716

Protein sequence:

>716_residues
MIQMPKKNKFMNRGAAILSICFALFFFVILGRMAYIQITGKANGEVLATKATEQHEKKRTIEASRGSILDRKGKVIAEDT
ATYKLIAILDKKMTTDVKHPQHVVNKEKTAEALSKVINLDKADILDILNKDAKQVEFGSAGRDITYSQKQKIEKMKLPGI
SFLRDTKRYYPNGVFASNLIGYAEVDEETNEISGAMGLEKVLDKYLKERDGYVTYESDKSGWELPNSKNKITAPKNGDNV
YLTIDQKIQTFLEDSMTKVAQKYNPKKIMAAVVDPKTGKVLAMGQRPSFDPNKRDVTNYYNDLISYAYEPGSTMKIFTLA
AAMQENVFNANEKYKSGTFEVGGAPVKDHNNGVGWGPTTYHDGVLRSSNVAFAKLAKEKLGYDRLNQYLHKFNFYQKTGI
DLPGEVSSKINFKYEFDKASTAYGQASAVTPIQQIQAATAIANDGKMMKPYVIDHIVDPDKDKTIYQNKPESAGTPISAS
TAKKVRDILGEVVTSKIGTGQAYKIEGFDVAGKTGTAQIAGKGGYLDGTDNYIFSFMGMAPKDDPELLIYVAVQQPQLKA
GQSSSDPVSEIFNPTMKNSLHYLNIEPTEKSDSDKEETKAQTMPDLTDQTVAAAQKKAKEENLTPIVIGSDVAVKEQYPK
ADEEVLTNQKVFLKTGGKIKMPDMTGWSRREVLQYGELAGIHIEVSGQGYAVSQSVKKDKEIKDKTVIKVKFKNPD

Sequences:

>Translated_716_residues
MIQMPKKNKFMNRGAAILSICFALFFFVILGRMAYIQITGKANGEVLATKATEQHEKKRTIEASRGSILDRKGKVIAEDT
ATYKLIAILDKKMTTDVKHPQHVVNKEKTAEALSKVINLDKADILDILNKDAKQVEFGSAGRDITYSQKQKIEKMKLPGI
SFLRDTKRYYPNGVFASNLIGYAEVDEETNEISGAMGLEKVLDKYLKERDGYVTYESDKSGWELPNSKNKITAPKNGDNV
YLTIDQKIQTFLEDSMTKVAQKYNPKKIMAAVVDPKTGKVLAMGQRPSFDPNKRDVTNYYNDLISYAYEPGSTMKIFTLA
AAMQENVFNANEKYKSGTFEVGGAPVKDHNNGVGWGPTTYHDGVLRSSNVAFAKLAKEKLGYDRLNQYLHKFNFYQKTGI
DLPGEVSSKINFKYEFDKASTAYGQASAVTPIQQIQAATAIANDGKMMKPYVIDHIVDPDKDKTIYQNKPESAGTPISAS
TAKKVRDILGEVVTSKIGTGQAYKIEGFDVAGKTGTAQIAGKGGYLDGTDNYIFSFMGMAPKDDPELLIYVAVQQPQLKA
GQSSSDPVSEIFNPTMKNSLHYLNIEPTEKSDSDKEETKAQTMPDLTDQTVAAAQKKAKEENLTPIVIGSDVAVKEQYPK
ADEEVLTNQKVFLKTGGKIKMPDMTGWSRREVLQYGELAGIHIEVSGQGYAVSQSVKKDKEIKDKTVIKVKFKNPD
>Mature_716_residues
MIQMPKKNKFMNRGAAILSICFALFFFVILGRMAYIQITGKANGEVLATKATEQHEKKRTIEASRGSILDRKGKVIAEDT
ATYKLIAILDKKMTTDVKHPQHVVNKEKTAEALSKVINLDKADILDILNKDAKQVEFGSAGRDITYSQKQKIEKMKLPGI
SFLRDTKRYYPNGVFASNLIGYAEVDEETNEISGAMGLEKVLDKYLKERDGYVTYESDKSGWELPNSKNKITAPKNGDNV
YLTIDQKIQTFLEDSMTKVAQKYNPKKIMAAVVDPKTGKVLAMGQRPSFDPNKRDVTNYYNDLISYAYEPGSTMKIFTLA
AAMQENVFNANEKYKSGTFEVGGAPVKDHNNGVGWGPTTYHDGVLRSSNVAFAKLAKEKLGYDRLNQYLHKFNFYQKTGI
DLPGEVSSKINFKYEFDKASTAYGQASAVTPIQQIQAATAIANDGKMMKPYVIDHIVDPDKDKTIYQNKPESAGTPISAS
TAKKVRDILGEVVTSKIGTGQAYKIEGFDVAGKTGTAQIAGKGGYLDGTDNYIFSFMGMAPKDDPELLIYVAVQQPQLKA
GQSSSDPVSEIFNPTMKNSLHYLNIEPTEKSDSDKEETKAQTMPDLTDQTVAAAQKKAKEENLTPIVIGSDVAVKEQYPK
ADEEVLTNQKVFLKTGGKIKMPDMTGWSRREVLQYGELAGIHIEVSGQGYAVSQSVKKDKEIKDKTVIKVKFKNPD

Specific function: Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. PBP-2B is required for vegetative cell division and sporulation septation. Beta-lactamase inactivates the PBPs by acylating an essential serine residue in the active sit

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PASTA domains

Homologues:

Organism=Escherichia coli, GI1786272, Length=610, Percent_Identity=26.8852459016393, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1786854, Length=560, Percent_Identity=25.1785714285714, Blast_Score=136, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PBPB_BACSU (Q07868)

Other databases:

- EMBL:   L09703
- EMBL:   Z68230
- EMBL:   AL009126
- EMBL:   Z25865
- PIR:   C53292
- RefSeq:   NP_389399.2
- ProteinModelPortal:   Q07868
- SMR:   Q07868
- EnsemblBacteria:   EBBACT00000000382
- GeneID:   939847
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU15160
- NMPDR:   fig|224308.1.peg.1518
- GenoList:   BSU15160
- GeneTree:   EBGT00070000031885
- HOGENOM:   HBG712588
- ProtClustDB:   CLSK887268
- BioCyc:   BSUB:BSU15160-MONOMER
- InterPro:   IPR012338
- InterPro:   IPR005543
- InterPro:   IPR005311
- InterPro:   IPR001460
- SMART:   SM00740

Pfam domain/function: PF03793 PASTA; PF03717 PBP_dimer; PF00905 Transpeptidase; SSF56519 PBP_dimer; SSF56601 PBP_transp_fold

EC number: NA

Molecular weight: Translated: 79321; Mature: 79321

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS51178 PASTA

Important sites: ACT_SITE 312-312

Signals:

None

Transmembrane regions:

HASH(0xe2cb8f0)-;

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQMPKKNKFMNRGAAILSICFALFFFVILGRMAYIQITGKANGEVLATKATEQHEKKRT
CCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCEEEECCHHHHHHHHH
IEASRGSILDRKGKVIAEDTATYKLIAILDKKMTTDVKHPQHVVNKEKTAEALSKVINLD
HHHHCCCCCCCCCCEEEECCCCEEEEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
KADILDILNKDAKQVEFGSAGRDITYSQKQKIEKMKLPGISFLRDTKRYYPNGVFASNLI
HHHHHHHHCCCHHHEECCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHCC
GYAEVDEETNEISGAMGLEKVLDKYLKERDGYVTYESDKSGWELPNSKNKITAPKNGDNV
CHHHCCCHHHHHHHHCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCEECCCCCCEE
YLTIDQKIQTFLEDSMTKVAQKYNPKKIMAAVVDPKTGKVLAMGQRPSFDPNKRDVTNYY
EEEECHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCCCCEEEECCCCCCCCCCHHHHHHH
NDLISYAYEPGSTMKIFTLAAAMQENVFNANEKYKSGTFEVGGAPVKDHNNGVGWGPTTY
HHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCC
HDGVLRSSNVAFAKLAKEKLGYDRLNQYLHKFNFYQKTGIDLPGEVSSKINFKYEFDKAS
CCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCHHHCCCCCCCCCCCCEEEEEEECCCC
TAYGQASAVTPIQQIQAATAIANDGKMMKPYVIDHIVDPDKDKTIYQNKPESAGTPISAS
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHH
TAKKVRDILGEVVTSKIGTGQAYKIEGFDVAGKTGTAQIAGKGGYLDGTDNYIFSFMGMA
HHHHHHHHHHHHHHHHCCCCCEEEECCEEECCCCCCEEEECCCCCCCCCCHHHHHCCCCC
PKDDPELLIYVAVQQPQLKAGQSSSDPVSEIFNPTMKNSLHYLNIEPTEKSDSDKEETKA
CCCCCCEEEEEEECCCCCCCCCCCCCCHHHHCCHHHHCCEEEEEECCCCCCCCCHHHHHH
QTMPDLTDQTVAAAQKKAKEENLTPIVIGSDVAVKEQYPKADEEVLTNQKVFLKTGGKIK
HCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCCHHHHHCCCEEEEECCCEEE
MPDMTGWSRREVLQYGELAGIHIEVSGQGYAVSQSVKKDKEIKDKTVIKVKFKNPD
CCCCCCCHHHHHHHHCCEEEEEEEECCCCEEEHHHHHHHHCCCCCEEEEEEECCCH
>Mature Secondary Structure
MIQMPKKNKFMNRGAAILSICFALFFFVILGRMAYIQITGKANGEVLATKATEQHEKKRT
CCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCEEEECCHHHHHHHHH
IEASRGSILDRKGKVIAEDTATYKLIAILDKKMTTDVKHPQHVVNKEKTAEALSKVINLD
HHHHCCCCCCCCCCEEEECCCCEEEEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
KADILDILNKDAKQVEFGSAGRDITYSQKQKIEKMKLPGISFLRDTKRYYPNGVFASNLI
HHHHHHHHCCCHHHEECCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHCC
GYAEVDEETNEISGAMGLEKVLDKYLKERDGYVTYESDKSGWELPNSKNKITAPKNGDNV
CHHHCCCHHHHHHHHCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCEECCCCCCEE
YLTIDQKIQTFLEDSMTKVAQKYNPKKIMAAVVDPKTGKVLAMGQRPSFDPNKRDVTNYY
EEEECHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCCCCEEEECCCCCCCCCCHHHHHHH
NDLISYAYEPGSTMKIFTLAAAMQENVFNANEKYKSGTFEVGGAPVKDHNNGVGWGPTTY
HHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCC
HDGVLRSSNVAFAKLAKEKLGYDRLNQYLHKFNFYQKTGIDLPGEVSSKINFKYEFDKAS
CCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCHHHCCCCCCCCCCCCEEEEEEECCCC
TAYGQASAVTPIQQIQAATAIANDGKMMKPYVIDHIVDPDKDKTIYQNKPESAGTPISAS
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHH
TAKKVRDILGEVVTSKIGTGQAYKIEGFDVAGKTGTAQIAGKGGYLDGTDNYIFSFMGMA
HHHHHHHHHHHHHHHHCCCCCEEEECCEEECCCCCCEEEECCCCCCCCCCHHHHHCCCCC
PKDDPELLIYVAVQQPQLKAGQSSSDPVSEIFNPTMKNSLHYLNIEPTEKSDSDKEETKA
CCCCCCEEEEEEECCCCCCCCCCCCCCHHHHCCHHHHCCEEEEEECCCCCCCCCHHHHHH
QTMPDLTDQTVAAAQKKAKEENLTPIVIGSDVAVKEQYPKADEEVLTNQKVFLKTGGKIK
HCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCCHHHHHCCCEEEEECCCEEE
MPDMTGWSRREVLQYGELAGIHIEVSGQGYAVSQSVKKDKEIKDKTVIKVKFKNPD
CCCCCCCHHHHHHHHCCEEEEEEEECCCCEEEHHHHHHHHCCCCCEEEEEEECCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8244929; 9384377; 8289242