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The map label for this gene is metE

Identifier: 255767311

GI number: 255767311

Start: 1383320

End: 1385608

Strand: Reverse

Name: metE

Synonym: BSU13180

Alternate gene names: 255767311

Gene position: 1385608-1383320 (Counterclockwise)

Preceding gene: 16078384

Following gene: 16078382

Centisome position: 32.87

GC content: 45.26

Gene sequence:

>2289_bases
ATGACAACCATCAAAACATCGAATTTAGGATTTCCGAGAATCGGACTGAACCGGGAATGGAAAAAAGCACTTGAAGCGTA
TTGGAAAGGCAGTACTGATAAAGATACGTTTTTGAAGCAAATCGACGAACTATTTTTATCCGCAGTAAAAACACAAATTG
ACCAGCAGATTGATGTTGTGCCTGTTTCTGATTTCACACAGTATGACCATGTACTCGACACAGCAGTCAGCTTCAACTGG
ATCCCGAAACGGTTCAGACATTTGACTGACGCTACCGATACATACTTCGCTATCGCCCGCGGAATCAAAGACGCTGTATC
TAGTGAAATGACAAAATGGTTTAATACAAATTACCATTACATCGTTCCGGAATATGACGAGAGCATTGAGTTCCGTCTGA
CAAGAAACAAACAACTCGAAGATTATCGCCGGATCAAACAGGAATACGGTGTGGAAACAAAGCCTGTGATTGTCGGCCCT
TATACGTTCGTTACGCTTGCTAAAGGCTATGAACCGTCTGAAGCAAAAGCGATCCAAAAACGCCTTGTGCCATTATATGT
ACAGCTTTTGAAAGAGCTTGAAGAAGAAGGCGTAAAATGGGTTCAAATCGATGAGCCGGCGCTCGTTACCGCCTCTAGTG
AAGATGTACGCGGCGCAAAAGAATTATTTGAAAGCATTACAAGTGAGCTTTCATCCTTGAATGTGCTTTTGCAGACGTAT
TTTGATTCTGTTGATGCTTATGAAGAGCTGATCTCTTACCCGGTTCAGGGAATTGGCCTTGATTTCGTTCACGACAAAGG
CAGAAACCTGGAACAGCTTAAAACACATGGCTTCCCGACAGATAAAGTGCTGGCAGCCGGCGTTATCGACGGACGCAACA
TTTGGAAAGCGGACCTTGAAGAGCGTCTCGATGCCGTTCTTGATATTCTCAGCATTGCAAAAGTTGATGAACTGTGGATT
CAGCCTTCCAGCAGCCTGCTGCATGTTCCAGTAGCGAAACACCCTGATGAGCATTTGGAAAAAGACCTATTGAACGGATT
ATCCTACGCAAAAGAAAAGCTGGCCGAGCTGACAGCTTTGAAAGAAGGCTTAGTATCAGGAAAAGCGGCGATCAGCGAAG
AGATTCAGCAGGCTAAGGCTGATATCCAGGCGCTTAAACAGTTTGCAACAGGCGCCAATTCTGAACAAAAGAAAGAGCTT
GAGCAATTAACTGATAAAGACTTCAAGCGCCCGATTCCGTTTGAAGAACGTTTAGCCCTACAAAATGAATCTCTCGGCCT
TCCGCTTTTGCCGACGACAACGATCGGCAGCTTCCCGCAGTCTGCTGAAGTGCGGAGCGCACGCCAAAAATGGCGGAAAG
CTGAGTGGTCCGATGAACAGTATCAAAACTTTATCAATGCGGAAACAAAAAGATGGATTGATATTCAGGAAGAATTGGAG
CTTGATGTCCTTGTTCACGGCGAATTTGAACGGACAGACATGGTCGAATACTTCGGTGAAAAGCTGGCCGGTTTCGCCTT
CACTAAATATGCCTGGGTTCAATCATACGGCTCACGCTGTGTCCGCCCGCCAGTCATTTACGGAGATGTTGAATTTATTG
AACCGATGACAGTGAAAGACACAGTCTACGCACAGTCATTGACTTCCAAGCATGTGAAAGGAATGCTGACGGGCCCGGTT
ACAATCTTAAACTGGTCTTTCCCTCGAAACGACATCTCGAGGAAAGAAATCGCCTTCCAAATCGGGCTTGCCCTTCGCAA
AGAAGTTAAAGCGCTTGAAGACGCAGGCATTCAAATCATTCAAGTCGATGAACCAGCGCTGCGTGAAGGCCTTCCATTGA
AAACCCGCGATTGGGATGAGTATTTGACTTGGGCGGCAGAAGCTTTCAGATTAACCACTTCTTCCGTGAAAAACGAGACA
CAAATTCATACACATATGTGCTACAGCAACTTCGAAGATATCGTTGATACAATCAATGATCTTGATGCCGATGTGATTAC
AATCGAACATAGCAGAAGCCACGGAGGATTTTTAGATTACTTAAAAAACCACCCGTATTTGAAAGGGCTTGGCCTTGGTG
TATATGACATTCACAGCCCTCGTGTGCCGTCAACTGAAGAAATGTACAATATTATCGTTGATGCGCTTGCCGTCTGTCCG
ACTGACCGCTTCTGGGTAAATCCAGACTGCGGATTGAAAACAAGACAGCAGGAAGAAACGGTTGCAGCATTGAAAAATAT
GGTTGAAGCCGCAAAACAGGCAAGAGCACAGCAGACACAGCTAGTATAA

Upstream 100 bases:

>100_bases
TTTCTCTCATGGGAAAGAGGCTTTTTGTTGTGAGAAAACCTCTTAGCAGCCTGTATCCGCGGGTGAAAGAGAGTGTTTTA
CATATAAAGGAGGAGAAACA

Downstream 100 bases:

>100_bases
TTTGAAAAAACCATCTGCATTTGGCAGATGGTTTTTTTCTATAATACAGCCACAATCGGTTTCTTATTTAGCAAATCCCC
CAAATACTTTGTTTATTTTG

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme; Superoxide-inducible protein 9; SOI9

Number of amino acids: Translated: 762; Mature: 761

Protein sequence:

>762_residues
MTTIKTSNLGFPRIGLNREWKKALEAYWKGSTDKDTFLKQIDELFLSAVKTQIDQQIDVVPVSDFTQYDHVLDTAVSFNW
IPKRFRHLTDATDTYFAIARGIKDAVSSEMTKWFNTNYHYIVPEYDESIEFRLTRNKQLEDYRRIKQEYGVETKPVIVGP
YTFVTLAKGYEPSEAKAIQKRLVPLYVQLLKELEEEGVKWVQIDEPALVTASSEDVRGAKELFESITSELSSLNVLLQTY
FDSVDAYEELISYPVQGIGLDFVHDKGRNLEQLKTHGFPTDKVLAAGVIDGRNIWKADLEERLDAVLDILSIAKVDELWI
QPSSSLLHVPVAKHPDEHLEKDLLNGLSYAKEKLAELTALKEGLVSGKAAISEEIQQAKADIQALKQFATGANSEQKKEL
EQLTDKDFKRPIPFEERLALQNESLGLPLLPTTTIGSFPQSAEVRSARQKWRKAEWSDEQYQNFINAETKRWIDIQEELE
LDVLVHGEFERTDMVEYFGEKLAGFAFTKYAWVQSYGSRCVRPPVIYGDVEFIEPMTVKDTVYAQSLTSKHVKGMLTGPV
TILNWSFPRNDISRKEIAFQIGLALRKEVKALEDAGIQIIQVDEPALREGLPLKTRDWDEYLTWAAEAFRLTTSSVKNET
QIHTHMCYSNFEDIVDTINDLDADVITIEHSRSHGGFLDYLKNHPYLKGLGLGVYDIHSPRVPSTEEMYNIIVDALAVCP
TDRFWVNPDCGLKTRQQEETVAALKNMVEAAKQARAQQTQLV

Sequences:

>Translated_762_residues
MTTIKTSNLGFPRIGLNREWKKALEAYWKGSTDKDTFLKQIDELFLSAVKTQIDQQIDVVPVSDFTQYDHVLDTAVSFNW
IPKRFRHLTDATDTYFAIARGIKDAVSSEMTKWFNTNYHYIVPEYDESIEFRLTRNKQLEDYRRIKQEYGVETKPVIVGP
YTFVTLAKGYEPSEAKAIQKRLVPLYVQLLKELEEEGVKWVQIDEPALVTASSEDVRGAKELFESITSELSSLNVLLQTY
FDSVDAYEELISYPVQGIGLDFVHDKGRNLEQLKTHGFPTDKVLAAGVIDGRNIWKADLEERLDAVLDILSIAKVDELWI
QPSSSLLHVPVAKHPDEHLEKDLLNGLSYAKEKLAELTALKEGLVSGKAAISEEIQQAKADIQALKQFATGANSEQKKEL
EQLTDKDFKRPIPFEERLALQNESLGLPLLPTTTIGSFPQSAEVRSARQKWRKAEWSDEQYQNFINAETKRWIDIQEELE
LDVLVHGEFERTDMVEYFGEKLAGFAFTKYAWVQSYGSRCVRPPVIYGDVEFIEPMTVKDTVYAQSLTSKHVKGMLTGPV
TILNWSFPRNDISRKEIAFQIGLALRKEVKALEDAGIQIIQVDEPALREGLPLKTRDWDEYLTWAAEAFRLTTSSVKNET
QIHTHMCYSNFEDIVDTINDLDADVITIEHSRSHGGFLDYLKNHPYLKGLGLGVYDIHSPRVPSTEEMYNIIVDALAVCP
TDRFWVNPDCGLKTRQQEETVAALKNMVEAAKQARAQQTQLV
>Mature_761_residues
TTIKTSNLGFPRIGLNREWKKALEAYWKGSTDKDTFLKQIDELFLSAVKTQIDQQIDVVPVSDFTQYDHVLDTAVSFNWI
PKRFRHLTDATDTYFAIARGIKDAVSSEMTKWFNTNYHYIVPEYDESIEFRLTRNKQLEDYRRIKQEYGVETKPVIVGPY
TFVTLAKGYEPSEAKAIQKRLVPLYVQLLKELEEEGVKWVQIDEPALVTASSEDVRGAKELFESITSELSSLNVLLQTYF
DSVDAYEELISYPVQGIGLDFVHDKGRNLEQLKTHGFPTDKVLAAGVIDGRNIWKADLEERLDAVLDILSIAKVDELWIQ
PSSSLLHVPVAKHPDEHLEKDLLNGLSYAKEKLAELTALKEGLVSGKAAISEEIQQAKADIQALKQFATGANSEQKKELE
QLTDKDFKRPIPFEERLALQNESLGLPLLPTTTIGSFPQSAEVRSARQKWRKAEWSDEQYQNFINAETKRWIDIQEELEL
DVLVHGEFERTDMVEYFGEKLAGFAFTKYAWVQSYGSRCVRPPVIYGDVEFIEPMTVKDTVYAQSLTSKHVKGMLTGPVT
ILNWSFPRNDISRKEIAFQIGLALRKEVKALEDAGIQIIQVDEPALREGLPLKTRDWDEYLTWAAEAFRLTTSSVKNETQ
IHTHMCYSNFEDIVDTINDLDADVITIEHSRSHGGFLDYLKNHPYLKGLGLGVYDIHSPRVPSTEEMYNIIVDALAVCPT
DRFWVNPDCGLKTRQQEETVAALKNMVEAAKQARAQQTQLV

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family

Homologues:

Organism=Escherichia coli, GI2367304, Length=762, Percent_Identity=46.1942257217848, Blast_Score=630, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=775, Percent_Identity=42.8387096774194, Blast_Score=588, Evalue=1e-168,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METE_BACSU (P80877)

Other databases:

- EMBL:   AJ002571
- EMBL:   AL009126
- PIR:   C69657
- RefSeq:   NP_389201.2
- ProteinModelPortal:   P80877
- SMR:   P80877
- EnsemblBacteria:   EBBACT00000003928
- GeneID:   936480
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU13180
- NMPDR:   fig|224308.1.peg.1320
- GenoList:   BSU13180
- GeneTree:   EBGT00050000001686
- HOGENOM:   HBG287495
- ProtClustDB:   PRK05222
- BioCyc:   BSUB:BSU13180-MONOMER
- BioCyc:   MetaCyc:MONOMER-14559
- BRENDA:   2.1.1.14
- HAMAP:   MF_00172
- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629
- PIRSF:   PIRSF000382
- TIGRFAMs:   TIGR01371

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2

EC number: =2.1.1.14

Molecular weight: Translated: 86807; Mature: 86676

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIKTSNLGFPRIGLNREWKKALEAYWKGSTDKDTFLKQIDELFLSAVKTQIDQQIDVV
CCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
PVSDFTQYDHVLDTAVSFNWIPKRFRHLTDATDTYFAIARGIKDAVSSEMTKWFNTNYHY
ECCHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
IVPEYDESIEFRLTRNKQLEDYRRIKQEYGVETKPVIVGPYTFVTLAKGYEPSEAKAIQK
EECCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEEECCHHEEHEECCCCCHHHHHHHH
RLVPLYVQLLKELEEEGVKWVQIDEPALVTASSEDVRGAKELFESITSELSSLNVLLQTY
HHHHHHHHHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDSVDAYEELISYPVQGIGLDFVHDKGRNLEQLKTHGFPTDKVLAAGVIDGRNIWKADLE
HHHHHHHHHHHHCCHHHCCEEEECCCCCCHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHH
ERLDAVLDILSIAKVDELWIQPSSSLLHVPVAKHPDEHLEKDLLNGLSYAKEKLAELTAL
HHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KEGLVSGKAAISEEIQQAKADIQALKQFATGANSEQKKELEQLTDKDFKRPIPFEERLAL
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHH
QNESLGLPLLPTTTIGSFPQSAEVRSARQKWRKAEWSDEQYQNFINAETKRWIDIQEELE
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHCC
LDVLVHGEFERTDMVEYFGEKLAGFAFTKYAWVQSYGSRCVRPPVIYGDVEFIEPMTVKD
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHCCCCCHHH
TVYAQSLTSKHVKGMLTGPVTILNWSFPRNDISRKEIAFQIGLALRKEVKALEDAGIQII
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEE
QVDEPALREGLPLKTRDWDEYLTWAAEAFRLTTSSVKNETQIHTHMCYSNFEDIVDTIND
EECCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LDADVITIEHSRSHGGFLDYLKNHPYLKGLGLGVYDIHSPRVPSTEEMYNIIVDALAVCP
CCCCEEEEECCCCCCCHHHHHHCCCCEEECCCCEEECCCCCCCCHHHHHHHHHHHHHHCC
TDRFWVNPDCGLKTRQQEETVAALKNMVEAAKQARAQQTQLV
CCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTIKTSNLGFPRIGLNREWKKALEAYWKGSTDKDTFLKQIDELFLSAVKTQIDQQIDVV
CEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
PVSDFTQYDHVLDTAVSFNWIPKRFRHLTDATDTYFAIARGIKDAVSSEMTKWFNTNYHY
ECCHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
IVPEYDESIEFRLTRNKQLEDYRRIKQEYGVETKPVIVGPYTFVTLAKGYEPSEAKAIQK
EECCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEEECCHHEEHEECCCCCHHHHHHHH
RLVPLYVQLLKELEEEGVKWVQIDEPALVTASSEDVRGAKELFESITSELSSLNVLLQTY
HHHHHHHHHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDSVDAYEELISYPVQGIGLDFVHDKGRNLEQLKTHGFPTDKVLAAGVIDGRNIWKADLE
HHHHHHHHHHHHCCHHHCCEEEECCCCCCHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHH
ERLDAVLDILSIAKVDELWIQPSSSLLHVPVAKHPDEHLEKDLLNGLSYAKEKLAELTAL
HHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KEGLVSGKAAISEEIQQAKADIQALKQFATGANSEQKKELEQLTDKDFKRPIPFEERLAL
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHH
QNESLGLPLLPTTTIGSFPQSAEVRSARQKWRKAEWSDEQYQNFINAETKRWIDIQEELE
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHCC
LDVLVHGEFERTDMVEYFGEKLAGFAFTKYAWVQSYGSRCVRPPVIYGDVEFIEPMTVKD
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHCCCCCHHH
TVYAQSLTSKHVKGMLTGPVTILNWSFPRNDISRKEIAFQIGLALRKEVKALEDAGIQII
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEE
QVDEPALREGLPLKTRDWDEYLTWAAEAFRLTTSSVKNETQIHTHMCYSNFEDIVDTIND
EECCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LDADVITIEHSRSHGGFLDYLKNHPYLKGLGLGVYDIHSPRVPSTEEMYNIIVDALAVCP
CCCCEEEEECCCCCCCHHHHHHCCCCEEECCCCEEECCCCCCCCHHHHHHHHHHHHHHCC
TDRFWVNPDCGLKTRQQEETVAALKNMVEAAKQARAQQTQLV
CCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 9298659