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The map label for this gene is ykfC

Identifier: 255767304

GI number: 255767304

Start: 1367941

End: 1368831

Strand: Direct

Name: ykfC

Synonym: BSU12990

Alternate gene names: 255767304

Gene position: 1367941-1368831 (Clockwise)

Preceding gene: 16078363

Following gene: 255767305

Centisome position: 32.45

GC content: 47.47

Gene sequence:

>891_bases
ATGATGCACACTGTCATATCAGCAGTGGCCAACATCTGGACAGCGCCTGATTCACCTCGTCCGTCTGATCAATTCATGCT
TCAACCGACTGTAATGATCAGAGACTGGCTGGAGCGCATGACGTATGATGAACGGCTTGGATTATGTACAGACAATGTAA
TCCAAACTCAGGTTCTCTTTGGCGAAAAGGTACTTGTGACGGCGGAACAGGGGGAATGGGTTTCTGTGATCGTGCCTAGC
CAGCCATCCCGAAAGGATCCGCGCGGATACCCGGGCTGGATGAAAAAGTACCAGCTGGAAAAAACAAAGCCCATCCATAC
ACAACACGATGTGATGATCAGCAAACCTGCTGCCTTTTTGTACAGAAGCAATGGGGAAAAGGAGATCGAATTAAGCTTTT
TGACAGTTCTGCCCCTTATTGCAAAAGAAAACGGATATTTTAAGGTTTCGACCGTTTTTGGGGAAAGGTTTGTGAGGCAA
AGTGATGCAGTGCCTGTCAGCCAACAGAAAGGGACTGCTGAAGACATCATTCAAACGGGTGCGTTTTTTCTTGGCCTTCC
CTACCTGTGGGGAGGGATCAGCGGGTTTGGGTTTGATTGCTCCGGATTTATGTACAGTATATTTAAGGCGAATGGATACA
GCATCCCCCGTGATGCGGGAGATCAGGCTAAGGCAGGGAAGGGTGTCCCGCTTGATGATATGAAAGCCGGTGATCTGCTG
TTTTTTGCTTATGAGGAAGGAAAAGGAGCGATTCATCACGTCGGTCTGTATGTAGGCGGCGGGAAAATGCTTCATTCTCC
AAAGACAGGGAAGTCAATCGAAATCCTCACATTAACAGAGACAATCTATGAAAAAGAATTATGTGCGGTGCGCCGCTGTT
TTTCAGAATAA

Upstream 100 bases:

>100_bases
TCAATGGCGGCATAACATATAGCGGCAGCACGATTTCGATGCCTGGCAAACCGGGCCTCGGAATCATCGGGGCTGCGCTT
TTGAAAGGGGAAAAAGAGCA

Downstream 100 bases:

>100_bases
GGAGGGAGACAAATGAGGCGACTGCCTTTGTTAGAGGTCAGCCAGCTGAAAATGCATTTTGACGCAGGGAAAAAGCGGAC
AGTCAAAGCTGTCGACGGGG

Product: cell wall endopeptidase

Products: NA

Alternate protein names: Cell wall endopeptidase ykfC

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MMHTVISAVANIWTAPDSPRPSDQFMLQPTVMIRDWLERMTYDERLGLCTDNVIQTQVLFGEKVLVTAEQGEWVSVIVPS
QPSRKDPRGYPGWMKKYQLEKTKPIHTQHDVMISKPAAFLYRSNGEKEIELSFLTVLPLIAKENGYFKVSTVFGERFVRQ
SDAVPVSQQKGTAEDIIQTGAFFLGLPYLWGGISGFGFDCSGFMYSIFKANGYSIPRDAGDQAKAGKGVPLDDMKAGDLL
FFAYEEGKGAIHHVGLYVGGGKMLHSPKTGKSIEILTLTETIYEKELCAVRRCFSE

Sequences:

>Translated_296_residues
MMHTVISAVANIWTAPDSPRPSDQFMLQPTVMIRDWLERMTYDERLGLCTDNVIQTQVLFGEKVLVTAEQGEWVSVIVPS
QPSRKDPRGYPGWMKKYQLEKTKPIHTQHDVMISKPAAFLYRSNGEKEIELSFLTVLPLIAKENGYFKVSTVFGERFVRQ
SDAVPVSQQKGTAEDIIQTGAFFLGLPYLWGGISGFGFDCSGFMYSIFKANGYSIPRDAGDQAKAGKGVPLDDMKAGDLL
FFAYEEGKGAIHHVGLYVGGGKMLHSPKTGKSIEILTLTETIYEKELCAVRRCFSE
>Mature_296_residues
MMHTVISAVANIWTAPDSPRPSDQFMLQPTVMIRDWLERMTYDERLGLCTDNVIQTQVLFGEKVLVTAEQGEWVSVIVPS
QPSRKDPRGYPGWMKKYQLEKTKPIHTQHDVMISKPAAFLYRSNGEKEIELSFLTVLPLIAKENGYFKVSTVFGERFVRQ
SDAVPVSQQKGTAEDIIQTGAFFLGLPYLWGGISGFGFDCSGFMYSIFKANGYSIPRDAGDQAKAGKGVPLDDMKAGDLL
FFAYEEGKGAIHHVGLYVGGGKMLHSPKTGKSIEILTLTETIYEKELCAVRRCFSE

Specific function: Specifically hydrolyzes gamma-D-glutamyl-L-lysine bonds in murein peptides, releasing L-Ala-D-Glu

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family

Homologues:

Organism=Escherichia coli, GI1787944, Length=116, Percent_Identity=39.6551724137931, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1786421, Length=117, Percent_Identity=35.8974358974359, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788501, Length=123, Percent_Identity=32.520325203252, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788001, Length=109, Percent_Identity=31.1926605504587, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YKFC_BACSU (O35010)

Other databases:

- EMBL:   AJ002571
- EMBL:   AL009126
- PIR:   A69856
- RefSeq:   NP_389182.2
- ProteinModelPortal:   O35010
- SMR:   O35010
- MEROPS:   C40.001
- EnsemblBacteria:   EBBACT00000002588
- GeneID:   937979
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU12990
- NMPDR:   fig|224308.1.peg.1301
- GenoList:   BSU12990
- GeneTree:   EBGT00050000000160
- HOGENOM:   HBG337940
- ProtClustDB:   CLSK887158
- BioCyc:   BSUB:BSU12990-MONOMER
- InterPro:   IPR000064

Pfam domain/function: PF00877 NLPC_P60

EC number: NA

Molecular weight: Translated: 33025; Mature: 33025

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMHTVISAVANIWTAPDSPRPSDQFMLQPTVMIRDWLERMTYDERLGLCTDNVIQTQVLF
CHHHHHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHCCHHHHCCCCCCCHHHHHEEE
GEKVLVTAEQGEWVSVIVPSQPSRKDPRGYPGWMKKYQLEKTKPIHTQHDVMISKPAAFL
CCEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEECCCEEE
YRSNGEKEIELSFLTVLPLIAKENGYFKVSTVFGERFVRQSDAVPVSQQKGTAEDIIQTG
EECCCCCEEEEEHHHHHHHHCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
AFFLGLPYLWGGISGFGFDCSGFMYSIFKANGYSIPRDAGDQAKAGKGVPLDDMKAGDLL
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
FFAYEEGKGAIHHVGLYVGGGKMLHSPKTGKSIEILTLTETIYEKELCAVRRCFSE
EEEEECCCCCEEEEEEEECCCEEEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMHTVISAVANIWTAPDSPRPSDQFMLQPTVMIRDWLERMTYDERLGLCTDNVIQTQVLF
CHHHHHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHCCHHHHCCCCCCCHHHHHEEE
GEKVLVTAEQGEWVSVIVPSQPSRKDPRGYPGWMKKYQLEKTKPIHTQHDVMISKPAAFL
CCEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEECCCEEE
YRSNGEKEIELSFLTVLPLIAKENGYFKVSTVFGERFVRQSDAVPVSQQKGTAEDIIQTG
EECCCCCEEEEEHHHHHHHHCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
AFFLGLPYLWGGISGFGFDCSGFMYSIFKANGYSIPRDAGDQAKAGKGVPLDDMKAGDLL
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
FFAYEEGKGAIHHVGLYVGGGKMLHSPKTGKSIEILTLTETIYEKELCAVRRCFSE
EEEEECCCCCEEEEEEEECCCEEEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377