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The map label for this gene is pbpF

Identifier: 255767236

GI number: 255767236

Start: 1083851

End: 1085995

Strand: Direct

Name: pbpF

Synonym: BSU10110

Alternate gene names: 255767236

Gene position: 1083851-1085995 (Clockwise)

Preceding gene: 16078070

Following gene: 16078076

Centisome position: 25.71

GC content: 45.69

Gene sequence:

>2145_bases
ATGTTTAAGATAAAGAAAAAGAAACTTTTTATACCTATCATTATTTTAGTGTTAACTGCTTTTCTCGCTTTAATAGGATA
TATTTCAATTATTTTTCTCGGCCATTATGTCATAGATGAAAAAAAGCTTATTCTTCATGCTTCTTCAAAAATTGTTGATC
AAAACGGAGATGAGGTTGCAAGCCTGTATACAGAAAATCGCGAGCCGGTCTCGATCAATGAGATTCCTAAGCAGGTCAGA
GAAGCGTTTATCGCTGTTGAGGATAAAAGGTTCTATGAGCATCACGGCATTGATGCAAAATCCGTCGGAAGGGCAGTGTA
CCGCGATATATTAGCAGGCGGAAAGGTGGAAGGCGGAAGCACGATCACCCAGCAGCTTGCCAAAAATATTTTTCTGACAC
ATGACAAAACATTTCTAAGAAAAACAAAAGAAGTGATCATTGCGATTAATCTTGAACGGGATTACAGCAAGGACAAGCTG
CTTGAAATGTATTTAAACCAGCTTTACTTCGGACACGGGGTTTACGGCATACAAGCGGCGTCCCACTATTATTTTAATAA
AGAAGTAAAAGATTTGACTGTATCTGAAGGCGCTGTTTTGGCCGCTATTCCGAAGGCGCCTTCTACGTACTCTCCAATTT
TGCACCCTGATAAAAATAAGGAGCGGCGTGATACAATTCTAGGTATGATGAATGACCAAGGCTATATCAGTGCGAAAGAG
GCTGTCACCGCACAGGGCCGGACGCTTGGACTGCATGTGAAGAAACAGTCGGAAACACCTTGGTTTGACAGCTACATTGA
CCTTGTGATCAAAGAGGCGGAAGACAAGTATTCCATTTCCGGAGAGCAGCTTCTTCAAGGCGGATACACGATCAAGGTGC
CGCTCGATTCGAAGCTGCAGAAGACGGCTTACCAGGTGATGAAAGAGGGAAGCTATTATCCCGGAACAGATCAGAATGCC
GAGGGAAGCGCGGTCTTTATTAATAACAAGACAGGCGGTGTAGAAGCGGCCATCGGCGGAAGGGATTACACATCCAAAGG
ATATAACCGTGTCACAGCTGTCCGCCAGCCGGGTTCCACATTTAAGCCGCTTGCTGTATACGGACCGGCGATGCAGGAAA
AGAAATTTAAGCCATATTCTCTATTGAAGGATGAGTTGCAGTCCTACGGAGATTATACGCCGAAAAACTACGACAGCCGG
TATGAGGGCGAAGTCACTATGTCTGACGCCATTACGTACAGCAAAAACGCGCCGGCTGTCTGGACGCTGAATGAGATCGG
CGTCGAAACAGGGAAATCTTATTTGAAAGCAAACGGCATTGATATCCCTGATGAAGGCCTTGCTCTTGCTCTAGGCGGCT
TGGAAAAAGGCGTTTCACCGCTCCAGCTTGCCGGCGCGTTTCATACATTTGCCGCGAATGGAACGTATACTGAACCCTTC
TTTATCTCGAGCATAATAGATGAAGACGGAGAAACAATAGCCGATCATAAAGAAGAAGGAAAGCGGGTATTCAGCAAGCA
GACGTCTTGGAATATGACGAGAATGCTGCAGCAGGTAGTCAAAAAGGGAACGGCCACGTCCGGCACCTATCACGGCGACC
TGGCGGGAAAAACAGGTTCAACATCTTATACAGGCGTCTCCGGCGCGACAAAGGACGCCTGGTTTGCAGGCTATACGCCG
AAAATAACCGGGGCAGTCTGGATGGGCTATGACAAAACGGATCAAAACCATTATTTGAAAGCCGGGAGCTCATACCCGAC
GCGGCTGTTTAAGGATATTTTGACACAGGCAGGAGAAACCGGTCATGTGTTCACAAAGCCTAAAAACGTTAAAGAACTGG
AAAGCCCGATTGAGCTGAAGCCGGTCAAAACACTCACAGCCGATTACACATTTAAAGCAGCGGGGCTCTTTACTATTGAA
TTAAAGTGGGACGCTCAAGAGGATGATCGGGCCGTATACAGAATTTATGTAAACAAGGACGGCGAGGAGACGCTTCTTGA
TTCGGTTGAAGGGAAGGGAAGCTATGAAATTCCTTATGCCAACTTATTTTCGGGCGCGTCTTATAAAATTGTGCCTTATA
ATACACAGACAAAAAGAGAAGGGGAAGGAACCGATTACGTTCAGCCAAAATTGTTTTCCTCTTAA

Upstream 100 bases:

>100_bases
AGTATATCAAAACAATGGTATAAGTTTCTATTGGCGAGTGCTTCGAACATAATTCCAAACAATTTTTCCCATACTACCTT
TAGAAAGGCGAGGTGAGTTC

Downstream 100 bases:

>100_bases
TGGAATTCGGCGATTTTTTGAACTTTGAGCTACATTTCTATACCATTTGCCTAAAACAAGGTATAGTGTAATAAGTCAAG
AAATACGAACGTATGGTTGC

Product: penicillin-binding protein 2C required for spore germination

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F

Number of amino acids: Translated: 714; Mature: 714

Protein sequence:

>714_residues
MFKIKKKKLFIPIIILVLTAFLALIGYISIIFLGHYVIDEKKLILHASSKIVDQNGDEVASLYTENREPVSINEIPKQVR
EAFIAVEDKRFYEHHGIDAKSVGRAVYRDILAGGKVEGGSTITQQLAKNIFLTHDKTFLRKTKEVIIAINLERDYSKDKL
LEMYLNQLYFGHGVYGIQAASHYYFNKEVKDLTVSEGAVLAAIPKAPSTYSPILHPDKNKERRDTILGMMNDQGYISAKE
AVTAQGRTLGLHVKKQSETPWFDSYIDLVIKEAEDKYSISGEQLLQGGYTIKVPLDSKLQKTAYQVMKEGSYYPGTDQNA
EGSAVFINNKTGGVEAAIGGRDYTSKGYNRVTAVRQPGSTFKPLAVYGPAMQEKKFKPYSLLKDELQSYGDYTPKNYDSR
YEGEVTMSDAITYSKNAPAVWTLNEIGVETGKSYLKANGIDIPDEGLALALGGLEKGVSPLQLAGAFHTFAANGTYTEPF
FISSIIDEDGETIADHKEEGKRVFSKQTSWNMTRMLQQVVKKGTATSGTYHGDLAGKTGSTSYTGVSGATKDAWFAGYTP
KITGAVWMGYDKTDQNHYLKAGSSYPTRLFKDILTQAGETGHVFTKPKNVKELESPIELKPVKTLTADYTFKAAGLFTIE
LKWDAQEDDRAVYRIYVNKDGEETLLDSVEGKGSYEIPYANLFSGASYKIVPYNTQTKREGEGTDYVQPKLFSS

Sequences:

>Translated_714_residues
MFKIKKKKLFIPIIILVLTAFLALIGYISIIFLGHYVIDEKKLILHASSKIVDQNGDEVASLYTENREPVSINEIPKQVR
EAFIAVEDKRFYEHHGIDAKSVGRAVYRDILAGGKVEGGSTITQQLAKNIFLTHDKTFLRKTKEVIIAINLERDYSKDKL
LEMYLNQLYFGHGVYGIQAASHYYFNKEVKDLTVSEGAVLAAIPKAPSTYSPILHPDKNKERRDTILGMMNDQGYISAKE
AVTAQGRTLGLHVKKQSETPWFDSYIDLVIKEAEDKYSISGEQLLQGGYTIKVPLDSKLQKTAYQVMKEGSYYPGTDQNA
EGSAVFINNKTGGVEAAIGGRDYTSKGYNRVTAVRQPGSTFKPLAVYGPAMQEKKFKPYSLLKDELQSYGDYTPKNYDSR
YEGEVTMSDAITYSKNAPAVWTLNEIGVETGKSYLKANGIDIPDEGLALALGGLEKGVSPLQLAGAFHTFAANGTYTEPF
FISSIIDEDGETIADHKEEGKRVFSKQTSWNMTRMLQQVVKKGTATSGTYHGDLAGKTGSTSYTGVSGATKDAWFAGYTP
KITGAVWMGYDKTDQNHYLKAGSSYPTRLFKDILTQAGETGHVFTKPKNVKELESPIELKPVKTLTADYTFKAAGLFTIE
LKWDAQEDDRAVYRIYVNKDGEETLLDSVEGKGSYEIPYANLFSGASYKIVPYNTQTKREGEGTDYVQPKLFSS
>Mature_714_residues
MFKIKKKKLFIPIIILVLTAFLALIGYISIIFLGHYVIDEKKLILHASSKIVDQNGDEVASLYTENREPVSINEIPKQVR
EAFIAVEDKRFYEHHGIDAKSVGRAVYRDILAGGKVEGGSTITQQLAKNIFLTHDKTFLRKTKEVIIAINLERDYSKDKL
LEMYLNQLYFGHGVYGIQAASHYYFNKEVKDLTVSEGAVLAAIPKAPSTYSPILHPDKNKERRDTILGMMNDQGYISAKE
AVTAQGRTLGLHVKKQSETPWFDSYIDLVIKEAEDKYSISGEQLLQGGYTIKVPLDSKLQKTAYQVMKEGSYYPGTDQNA
EGSAVFINNKTGGVEAAIGGRDYTSKGYNRVTAVRQPGSTFKPLAVYGPAMQEKKFKPYSLLKDELQSYGDYTPKNYDSR
YEGEVTMSDAITYSKNAPAVWTLNEIGVETGKSYLKANGIDIPDEGLALALGGLEKGVSPLQLAGAFHTFAANGTYTEPF
FISSIIDEDGETIADHKEEGKRVFSKQTSWNMTRMLQQVVKKGTATSGTYHGDLAGKTGSTSYTGVSGATKDAWFAGYTP
KITGAVWMGYDKTDQNHYLKAGSSYPTRLFKDILTQAGETGHVFTKPKNVKELESPIELKPVKTLTADYTFKAAGLFTIE
LKWDAQEDDRAVYRIYVNKDGEETLLDSVEGKGSYEIPYANLFSGASYKIVPYNTQTKREGEGTDYVQPKLFSS

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family

Homologues:

Organism=Escherichia coli, GI1786343, Length=581, Percent_Identity=29.6041308089501, Blast_Score=215, Evalue=8e-57,
Organism=Escherichia coli, GI87082258, Length=301, Percent_Identity=36.5448504983389, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI1788867, Length=578, Percent_Identity=27.5086505190311, Blast_Score=192, Evalue=6e-50,
Organism=Escherichia coli, GI1789601, Length=172, Percent_Identity=35.4651162790698, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PBPF_BACSU (P38050)

Other databases:

- EMBL:   Y14083
- EMBL:   AL009126
- EMBL:   L10630
- EMBL:   M97208
- PIR:   A40614
- RefSeq:   NP_388892.2
- ProteinModelPortal:   P38050
- SMR:   P38050
- EnsemblBacteria:   EBBACT00000002764
- GeneID:   939766
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU10110
- NMPDR:   fig|224308.1.peg.1011
- GenoList:   BSU10110
- GeneTree:   EBGT00070000031873
- HOGENOM:   HBG672432
- ProtClustDB:   CLSK872961
- BioCyc:   BSUB:BSU10110-MONOMER
- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460
- TIGRFAMs:   TIGR02074

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase; SSF56601 PBP_transp_fold

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 79266; Mature: 79266

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: NA

Important sites: ACT_SITE 359-359

Signals:

None

Transmembrane regions:

HASH(0xd5d3a48)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKIKKKKLFIPIIILVLTAFLALIGYISIIFLGHYVIDEKKLILHASSKIVDQNGDEVA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCCCCHHH
SLYTENREPVSINEIPKQVREAFIAVEDKRFYEHHGIDAKSVGRAVYRDILAGGKVEGGS
HHHHCCCCCCCHHHHHHHHHHHHEEECCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCH
TITQQLAKNIFLTHDKTFLRKTKEVIIAINLERDYSKDKLLEMYLNQLYFGHGVYGIQAA
HHHHHHHHHEEECCCHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEHH
SHYYFNKEVKDLTVSEGAVLAAIPKAPSTYSPILHPDKNKERRDTILGMMNDQGYISAKE
HEEEECCCHHEEEECCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHEEEECCCCCEEHHH
AVTAQGRTLGLHVKKQSETPWFDSYIDLVIKEAEDKYSISGEQLLQGGYTIKVPLDSKLQ
HHHCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCEEEECCCHHHH
KTAYQVMKEGSYYPGTDQNAEGSAVFINNKTGGVEAAIGGRDYTSKGYNRVTAVRQPGST
HHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCEEEEEECCCCC
FKPLAVYGPAMQEKKFKPYSLLKDELQSYGDYTPKNYDSRYEGEVTMSDAITYSKNAPAV
CCCCEEECCCHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCEEECCCCCEE
WTLNEIGVETGKSYLKANGIDIPDEGLALALGGLEKGVSPLQLAGAFHTFAANGTYTEPF
EEHHHHCHHHCCHHHEECCCCCCCCCCEEEECCHHHCCCHHHHHHHHHHEECCCCCCCCH
FISSIIDEDGETIADHKEEGKRVFSKQTSWNMTRMLQQVVKKGTATSGTYHGDLAGKTGS
HHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
TSYTGVSGATKDAWFAGYTPKITGAVWMGYDKTDQNHYLKAGSSYPTRLFKDILTQAGET
CCCCCCCCCCCCCEECCCCCCCCEEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHCCCC
GHVFTKPKNVKELESPIELKPVKTLTADYTFKAAGLFTIELKWDAQEDDRAVYRIYVNKD
CEEEECCCCHHHHCCCCCCCCCEEEECCCEEEECEEEEEEEEECCCCCCCEEEEEEECCC
GEETLLDSVEGKGSYEIPYANLFSGASYKIVPYNTQTKREGEGTDYVQPKLFSS
CHHHHHHCCCCCCCCCCCHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCH
>Mature Secondary Structure
MFKIKKKKLFIPIIILVLTAFLALIGYISIIFLGHYVIDEKKLILHASSKIVDQNGDEVA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCCCCHHH
SLYTENREPVSINEIPKQVREAFIAVEDKRFYEHHGIDAKSVGRAVYRDILAGGKVEGGS
HHHHCCCCCCCHHHHHHHHHHHHEEECCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCH
TITQQLAKNIFLTHDKTFLRKTKEVIIAINLERDYSKDKLLEMYLNQLYFGHGVYGIQAA
HHHHHHHHHEEECCCHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEHH
SHYYFNKEVKDLTVSEGAVLAAIPKAPSTYSPILHPDKNKERRDTILGMMNDQGYISAKE
HEEEECCCHHEEEECCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHEEEECCCCCEEHHH
AVTAQGRTLGLHVKKQSETPWFDSYIDLVIKEAEDKYSISGEQLLQGGYTIKVPLDSKLQ
HHHCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCEEEECCCHHHH
KTAYQVMKEGSYYPGTDQNAEGSAVFINNKTGGVEAAIGGRDYTSKGYNRVTAVRQPGST
HHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCEEEEEECCCCC
FKPLAVYGPAMQEKKFKPYSLLKDELQSYGDYTPKNYDSRYEGEVTMSDAITYSKNAPAV
CCCCEEECCCHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCEEECCCCCEE
WTLNEIGVETGKSYLKANGIDIPDEGLALALGGLEKGVSPLQLAGAFHTFAANGTYTEPF
EEHHHHCHHHCCHHHEECCCCCCCCCCEEEECCHHHCCCHHHHHHHHHHEECCCCCCCCH
FISSIIDEDGETIADHKEEGKRVFSKQTSWNMTRMLQQVVKKGTATSGTYHGDLAGKTGS
HHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
TSYTGVSGATKDAWFAGYTPKITGAVWMGYDKTDQNHYLKAGSSYPTRLFKDILTQAGET
CCCCCCCCCCCCCEECCCCCCCCEEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHCCCC
GHVFTKPKNVKELESPIELKPVKTLTADYTFKAAGLFTIELKWDAQEDDRAVYRIYVNKD
CEEEECCCCHHHHCCCCCCCCCEEEECCCEEEECEEEEEEEEECCCCCCCEEEEEEECCC
GEETLLDSVEGKGSYEIPYANLFSGASYKIVPYNTQTKREGEGTDYVQPKLFSS
CHHHHHHCCCCCCCCCCCHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957