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The map label for this gene is yhaR

Identifier: 255767227

GI number: 255767227

Start: 1061491

End: 1062258

Strand: Direct

Name: yhaR

Synonym: BSU09880

Alternate gene names: 255767227

Gene position: 1061491-1062258 (Clockwise)

Preceding gene: 16081159

Following gene: 255767229

Centisome position: 25.18

GC content: 49.48

Gene sequence:

>768_bases
ATGGAGTTTGTTCAATATGCATGTAATGGGGCCGTTGCAGAGATTATCCTGAATCGGCCTGATGCCCATCACGCCCTGAA
TGAACAGATGCTGTCTGAATTGAAGGAGGCAGTCGAAATGGCGGCTGCAAGCGAGGCGCTCATCGTTCTCCTGAGAGGAA
GCGGGAAAGGCTTTTCGGCCGGCGGTGATATCAGGATGATGACGTCAGAGCATGACCCTGACCAATTTAAACGGCTGATG
GATACGATTGAGGCTGTCACCCTGAATTTATATCAGATGAAGAAAGTGACAATTGCAGCCATTCATGGTGCTGCTGCGGG
ACTGGGCCTGAGTTTGGCGCTTTGTGCGGACATTGTGCTCGCTGAAAAAAACGCCGTTCTCGCGATGAATTTTATCGGCA
TTGGGCTTGTTCCGGACGGCGGAGGACATTATTTGCTGAAGAAAAGAATCGGTGAAGCAAAAGCGAAAAAACTGATTTGG
AGCGGAAAAAAACTATCTGCCTCGGAAGCTGCCGATATGGGGCTTCTTGATGGGACATTTGCCGGAGACCCTGCCGAAGG
CGCAAGACCCATTATCGAAACCCTGTTGGCCTCCCCTCTTTTGGCGATGATCGAAACGAAAGGTATTTTTCAAAGCCTCC
AAATTGAAGAACTGAAAAAAGTGCTATCCCTTGAGCGCAGCGCCCAAGAGAGAATGAGAAGGACCAAGGATCACCAAGAA
GGAATCCGTGCCTTTTTAGAAAAACGGGAGCCGAAGTTTCAAGCGTAA

Upstream 100 bases:

>100_bases
CAGTATCTATTGACCTTGAAAAATAAAAAGGTTTATTTGGGGATGGCGGGGAATGTATAGGAGAAAGAAAAAAGTAAGCG
CATACATGGAGGTGCTTTTA

Downstream 100 bases:

>100_bases
CAGATCAGCCGGAAAGCTGATCATCTGTGAAACAAAATCAGCTTTCCGGCTGCATTGACTAAACGATGAGTATGACTATG
AAGCTCTTTTTCTTTTAAGA

Product: enoyl-CoA hydratase

Products: trans(or 3)-enoyl-CoA; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MEFVQYACNGAVAEIILNRPDAHHALNEQMLSELKEAVEMAAASEALIVLLRGSGKGFSAGGDIRMMTSEHDPDQFKRLM
DTIEAVTLNLYQMKKVTIAAIHGAAAGLGLSLALCADIVLAEKNAVLAMNFIGIGLVPDGGGHYLLKKRIGEAKAKKLIW
SGKKLSASEAADMGLLDGTFAGDPAEGARPIIETLLASPLLAMIETKGIFQSLQIEELKKVLSLERSAQERMRRTKDHQE
GIRAFLEKREPKFQA

Sequences:

>Translated_255_residues
MEFVQYACNGAVAEIILNRPDAHHALNEQMLSELKEAVEMAAASEALIVLLRGSGKGFSAGGDIRMMTSEHDPDQFKRLM
DTIEAVTLNLYQMKKVTIAAIHGAAAGLGLSLALCADIVLAEKNAVLAMNFIGIGLVPDGGGHYLLKKRIGEAKAKKLIW
SGKKLSASEAADMGLLDGTFAGDPAEGARPIIETLLASPLLAMIETKGIFQSLQIEELKKVLSLERSAQERMRRTKDHQE
GIRAFLEKREPKFQA
>Mature_255_residues
MEFVQYACNGAVAEIILNRPDAHHALNEQMLSELKEAVEMAAASEALIVLLRGSGKGFSAGGDIRMMTSEHDPDQFKRLM
DTIEAVTLNLYQMKKVTIAAIHGAAAGLGLSLALCADIVLAEKNAVLAMNFIGIGLVPDGGGHYLLKKRIGEAKAKKLIW
SGKKLSASEAADMGLLDGTFAGDPAEGARPIIETLLASPLLAMIETKGIFQSLQIEELKKVLSLERSAQERMRRTKDHQE
GIRAFLEKREPKFQA

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family

Homologues:

Organism=Homo sapiens, GI194097323, Length=256, Percent_Identity=30.46875, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI221136756, Length=162, Percent_Identity=32.0987654320988, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI221136753, Length=162, Percent_Identity=32.0987654320988, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI221307494, Length=162, Percent_Identity=32.0987654320988, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI4757968, Length=176, Percent_Identity=28.4090909090909, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI49355787, Length=176, Percent_Identity=28.4090909090909, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI4757966, Length=176, Percent_Identity=27.8409090909091, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI51538581, Length=176, Percent_Identity=27.8409090909091, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI25453481, Length=176, Percent_Identity=27.8409090909091, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI51538573, Length=176, Percent_Identity=27.8409090909091, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI260274832, Length=173, Percent_Identity=28.9017341040462, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI45643119, Length=174, Percent_Identity=29.8850574712644, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI260275230, Length=174, Percent_Identity=29.8850574712644, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI116235487, Length=161, Percent_Identity=30.4347826086957, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI68989263, Length=228, Percent_Identity=31.140350877193, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI31542718, Length=248, Percent_Identity=25.8064516129032, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI4502327, Length=261, Percent_Identity=30.2681992337165, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI20127408, Length=179, Percent_Identity=27.9329608938547, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI70995211, Length=181, Percent_Identity=29.8342541436464, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1787659, Length=242, Percent_Identity=32.6446280991736, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787660, Length=258, Percent_Identity=32.1705426356589, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI221142681, Length=259, Percent_Identity=27.7992277992278, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI87082183, Length=265, Percent_Identity=24.5283018867925, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788597, Length=249, Percent_Identity=24.8995983935743, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1788682, Length=208, Percent_Identity=25.9615384615385, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1790281, Length=217, Percent_Identity=23.963133640553, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17554946, Length=248, Percent_Identity=31.0483870967742, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI25145438, Length=251, Percent_Identity=32.2709163346614, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17535521, Length=247, Percent_Identity=25.1012145748988, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=185, Percent_Identity=29.7297297297297, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25141363, Length=211, Percent_Identity=24.1706161137441, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=242, Percent_Identity=27.6859504132231, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17534483, Length=180, Percent_Identity=29.4444444444444, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17558304, Length=173, Percent_Identity=26.5895953757225, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI20129971, Length=246, Percent_Identity=30.8943089430894, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24653477, Length=246, Percent_Identity=30.8943089430894, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24653139, Length=251, Percent_Identity=26.2948207171315, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24654903, Length=174, Percent_Identity=25.8620689655172, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19920382, Length=234, Percent_Identity=28.2051282051282, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI28571730, Length=139, Percent_Identity=32.3741007194245, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28571729, Length=139, Percent_Identity=32.3741007194245, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21357171, Length=245, Percent_Identity=27.7551020408163, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YHAR_BACSU (O07533)

Other databases:

- EMBL:   AL009126
- EMBL:   Y14078
- PIR:   D69819
- RefSeq:   NP_388869.3
- HSSP:   P14604
- ProteinModelPortal:   O07533
- SMR:   O07533
- EnsemblBacteria:   EBBACT00000000964
- GeneID:   939767
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU09880
- GenoList:   BSU09880
- GeneTree:   EBGT00070000031824
- HOGENOM:   HBG748731
- ProtClustDB:   PRK07659
- BioCyc:   BSUB:BSU09880-MONOMER
- InterPro:   IPR001753

Pfam domain/function: PF00378 ECH

EC number: 4.2.1.17 [C]

Molecular weight: Translated: 27611; Mature: 27611

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xb49bf1c)-; HASH(0xe11a6f0)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFVQYACNGAVAEIILNRPDAHHALNEQMLSELKEAVEMAAASEALIVLLRGSGKGFSA
CCHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCC
GGDIRMMTSEHDPDQFKRLMDTIEAVTLNLYQMKKVTIAAIHGAAAGLGLSLALCADIVL
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEKNAVLAMNFIGIGLVPDGGGHYLLKKRIGEAKAKKLIWSGKKLSASEAADMGLLDGTF
HCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCC
AGDPAEGARPIIETLLASPLLAMIETKGIFQSLQIEELKKVLSLERSAQERMRRTKDHQE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIRAFLEKREPKFQA
HHHHHHHHCCCCCCH
>Mature Secondary Structure
MEFVQYACNGAVAEIILNRPDAHHALNEQMLSELKEAVEMAAASEALIVLLRGSGKGFSA
CCHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCC
GGDIRMMTSEHDPDQFKRLMDTIEAVTLNLYQMKKVTIAAIHGAAAGLGLSLALCADIVL
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEKNAVLAMNFIGIGLVPDGGGHYLLKKRIGEAKAKKLIWSGKKLSASEAADMGLLDGTF
HCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCC
AGDPAEGARPIIETLLASPLLAMIETKGIFQSLQIEELKKVLSLERSAQERMRRTKDHQE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIRAFLEKREPKFQA
HHHHHHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-hydroxyacyl-CoA [C]

Specific reaction: (3S)-hydroxyacyl-CoA = trans(or 3)-enoyl-CoA + H2O [C]

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 9579061