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The map label for this gene is trmE

Identifier: 16081154

GI number: 16081154

Start: 4211510

End: 4212889

Strand: Reverse

Name: trmE

Synonym: BSU41020

Alternate gene names: 16081154

Gene position: 4212889-4211510 (Counterclockwise)

Preceding gene: 16081155

Following gene: 16081153

Centisome position: 99.94

GC content: 45.8

Gene sequence:

>1380_bases
ATGGATACAATTGCGGCAATTTCCACACCGATGGGAGAAGGCGCGATTGCGATTGTACGGTTGAGCGGACCAGAAGCCAT
TCAAATCGCAGATAAAATTTATAAGGGACCGAAGGGGAAAACACTCAGCTCGGTCGAATCACATACGATTCATTATGGTC
ATATCGTAGACAGACCTTCGGATCGTGTCGTTGAGGAAGTAATGGTATCAGTGTTAAAAGCGCCGAGAACTTTTACACGA
GAAGATGTCATCGAGATTAACTGTCACGGGGGAATTGTGACTGTGAACCAAGTGCTTCAGCTTGCATTGAGAGAGGGAGC
GAGACTGGCAGAGCCTGGTGAATTTACGAAACGTGCGTTTTTAAACGGCCGAATCGATCTTTCACAGGCGGAAGCGGTTA
TGGACTTAATCAGAGCGAAAACGGACCGGGCAATGAACGTTGCTATGAATCAAATGGAAGGGCGTCTTTCTGCTTTGGTG
CGCCGTTTGCGCAGTGAGATTTTAGAAACACTGGCCCATGTTGAGGTGAATATTGATTATCCGGAGTATGATGATGTAGA
GGAAATGACGCATCAGATTTTAGTCGAAAAGGCAACCGCCGTGAAAAAGGAAATTGAGACGCTGCTGAGGACTTCTGAGC
AAGGAAAAATCTTGCGTGAAGGTCTTTCTACCGTCATTATCGGCCGGCCAAACGTAGGAAAATCATCTCTTCTGAACAGC
CTCGTTCATGAGGCAAAAGCCATTGTAACCGATATTCCCGGAACGACACGGGATGTGATTGAGGAATACGTGAATGTAAG
GGGCGTTCCGCTTCGTCTGGTTGATACAGCAGGTATTCGTGAAACAGAGGATATTGTTGAGCGAATCGGTGTTGAACGTT
CTCGCCAGGTCCTAAAGGAAGCGGACCTGATTTTACTTGTGCTTAATTATAGTGAAGAGCTTTCTGAAGAAGACGTGAAG
CTTTTTGAAGCCGTTGAGGGCATGGATGTTATCGTGATTTTGAATAAGACTGACCTTGAGCCGAAGATTGACACTGAACG
TGTCCGTGAGCTTGCCAATGGACGTCCAGTTGTTACGACATCTCTTTTAAAAGAGGAAGGCATCAATGATCTGGAAGAAG
CGATTCAATCACTGTTCTACACGGGTGCCATTGAAAGCGGTGATCTGACTTATGTCAGCAACACACGTCACATCACTATT
TTACAGCAAGCAAAACGCGCCATTGAAGATGCGCTTAGCGGCATCGAACAGGATGTCCCGATCGACATGGTGCAAATTGA
CCTTACAAGATGCTGGGAGCTTTTAGGGGAAATCATTGGAGATTCTGTCCACGAAAGCTTAATTGATCAGCTCTTTTCTC
AATTCTGTTTAGGAAAATAA

Upstream 100 bases:

>100_bases
ACAAATCTTCCACTTCGAACCAAACTATGTTATCTTTTAGTTTTGGCATAATAAAAATTTTTTTATATAGATGAAGTATA
AGTAAAGAGAGGTGAACAAC

Downstream 100 bases:

>100_bases
TTAAAGGAGGAACTAGAATCATGGGGTATGAAGCAGGCCAATACGATGTGATTGTGATTGGCGCCGGGCATGCCGGTGTT
GAGGCTGCCCTCGCATCTGC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MDTIAAISTPMGEGAIAIVRLSGPEAIQIADKIYKGPKGKTLSSVESHTIHYGHIVDRPSDRVVEEVMVSVLKAPRTFTR
EDVIEINCHGGIVTVNQVLQLALREGARLAEPGEFTKRAFLNGRIDLSQAEAVMDLIRAKTDRAMNVAMNQMEGRLSALV
RRLRSEILETLAHVEVNIDYPEYDDVEEMTHQILVEKATAVKKEIETLLRTSEQGKILREGLSTVIIGRPNVGKSSLLNS
LVHEAKAIVTDIPGTTRDVIEEYVNVRGVPLRLVDTAGIRETEDIVERIGVERSRQVLKEADLILLVLNYSEELSEEDVK
LFEAVEGMDVIVILNKTDLEPKIDTERVRELANGRPVVTTSLLKEEGINDLEEAIQSLFYTGAIESGDLTYVSNTRHITI
LQQAKRAIEDALSGIEQDVPIDMVQIDLTRCWELLGEIIGDSVHESLIDQLFSQFCLGK

Sequences:

>Translated_459_residues
MDTIAAISTPMGEGAIAIVRLSGPEAIQIADKIYKGPKGKTLSSVESHTIHYGHIVDRPSDRVVEEVMVSVLKAPRTFTR
EDVIEINCHGGIVTVNQVLQLALREGARLAEPGEFTKRAFLNGRIDLSQAEAVMDLIRAKTDRAMNVAMNQMEGRLSALV
RRLRSEILETLAHVEVNIDYPEYDDVEEMTHQILVEKATAVKKEIETLLRTSEQGKILREGLSTVIIGRPNVGKSSLLNS
LVHEAKAIVTDIPGTTRDVIEEYVNVRGVPLRLVDTAGIRETEDIVERIGVERSRQVLKEADLILLVLNYSEELSEEDVK
LFEAVEGMDVIVILNKTDLEPKIDTERVRELANGRPVVTTSLLKEEGINDLEEAIQSLFYTGAIESGDLTYVSNTRHITI
LQQAKRAIEDALSGIEQDVPIDMVQIDLTRCWELLGEIIGDSVHESLIDQLFSQFCLGK
>Mature_459_residues
MDTIAAISTPMGEGAIAIVRLSGPEAIQIADKIYKGPKGKTLSSVESHTIHYGHIVDRPSDRVVEEVMVSVLKAPRTFTR
EDVIEINCHGGIVTVNQVLQLALREGARLAEPGEFTKRAFLNGRIDLSQAEAVMDLIRAKTDRAMNVAMNQMEGRLSALV
RRLRSEILETLAHVEVNIDYPEYDDVEEMTHQILVEKATAVKKEIETLLRTSEQGKILREGLSTVIIGRPNVGKSSLLNS
LVHEAKAIVTDIPGTTRDVIEEYVNVRGVPLRLVDTAGIRETEDIVERIGVERSRQVLKEADLILLVLNYSEELSEEDVK
LFEAVEGMDVIVILNKTDLEPKIDTERVRELANGRPVVTTSLLKEEGINDLEEAIQSLFYTGAIESGDLTYVSNTRHITI
LQQAKRAIEDALSGIEQDVPIDMVQIDLTRCWELLGEIIGDSVHESLIDQLFSQFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=470, Percent_Identity=34.0425531914894, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI193082988, Length=502, Percent_Identity=32.2709163346614, Blast_Score=208, Evalue=1e-53,
Organism=Homo sapiens, GI193082993, Length=459, Percent_Identity=33.3333333333333, Blast_Score=202, Evalue=5e-52,
Organism=Escherichia coli, GI2367268, Length=469, Percent_Identity=33.6886993603412, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI87082120, Length=166, Percent_Identity=32.5301204819277, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17507259, Length=467, Percent_Identity=30.8351177730193, Blast_Score=201, Evalue=8e-52,
Organism=Saccharomyces cerevisiae, GI6323665, Length=497, Percent_Identity=31.58953722334, Blast_Score=212, Evalue=1e-55,
Organism=Drosophila melanogaster, GI45550813, Length=492, Percent_Identity=33.130081300813, Blast_Score=211, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 50975; Mature: 50975

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTIAAISTPMGEGAIAIVRLSGPEAIQIADKIYKGPKGKTLSSVESHTIHYGHIVDRPS
CCCCHHCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHCCEEECEECCCCH
DRVVEEVMVSVLKAPRTFTREDVIEINCHGGIVTVNQVLQLALREGARLAEPGEFTKRAF
HHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEHHHHHHHHHHCCCCCCCCCHHHHHHH
LNGRIDLSQAEAVMDLIRAKTDRAMNVAMNQMEGRLSALVRRLRSEILETLAHVEVNIDY
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCC
PEYDDVEEMTHQILVEKATAVKKEIETLLRTSEQGKILREGLSTVIIGRPNVGKSSLLNS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEEEECCCCCHHHHHHH
LVHEAKAIVTDIPGTTRDVIEEYVNVRGVPLRLVDTAGIRETEDIVERIGVERSRQVLKE
HHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHH
ADLILLVLNYSEELSEEDVKLFEAVEGMDVIVILNKTDLEPKIDTERVRELANGRPVVTT
CCEEEEEECCCHHHCHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCEEHH
SLLKEEGINDLEEAIQSLFYTGAIESGDLTYVSNTRHITILQQAKRAIEDALSGIEQDVP
HHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCEEHHHHHHHHHHHHHHHCCCCCCC
IDMVQIDLTRCWELLGEIIGDSVHESLIDQLFSQFCLGK
CHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDTIAAISTPMGEGAIAIVRLSGPEAIQIADKIYKGPKGKTLSSVESHTIHYGHIVDRPS
CCCCHHCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHCCEEECEECCCCH
DRVVEEVMVSVLKAPRTFTREDVIEINCHGGIVTVNQVLQLALREGARLAEPGEFTKRAF
HHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEHHHHHHHHHHCCCCCCCCCHHHHHHH
LNGRIDLSQAEAVMDLIRAKTDRAMNVAMNQMEGRLSALVRRLRSEILETLAHVEVNIDY
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCC
PEYDDVEEMTHQILVEKATAVKKEIETLLRTSEQGKILREGLSTVIIGRPNVGKSSLLNS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEEEECCCCCHHHHHHH
LVHEAKAIVTDIPGTTRDVIEEYVNVRGVPLRLVDTAGIRETEDIVERIGVERSRQVLKE
HHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHH
ADLILLVLNYSEELSEEDVKLFEAVEGMDVIVILNKTDLEPKIDTERVRELANGRPVVTT
CCEEEEEECCCHHHCHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCEEHH
SLLKEEGINDLEEAIQSLFYTGAIESGDLTYVSNTRHITILQQAKRAIEDALSGIEQDVP
HHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCEEHHHHHHHHHHHHHHHCCCCCCC
IDMVQIDLTRCWELLGEIIGDSVHESLIDQLFSQFCLGK
CHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA