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The map label for this gene is tetB

Identifier: 16081129

GI number: 16081129

Start: 4187681

End: 4189057

Strand: Reverse

Name: tetB

Synonym: BSU40770

Alternate gene names: 16081129

Gene position: 4189057-4187681 (Counterclockwise)

Preceding gene: 16081130

Following gene: 16081126

Centisome position: 99.37

GC content: 39.94

Gene sequence:

>1377_bases
GTGAATACGTCTTATTCACAGTCAACTTTACGGCACAATCAAGTTTTGATTTGGCTTTGTGTTCTATCATTTTTCAGCGT
ATTAAATGAAATGGTTCTGAACGTCTCATTACCTGATATTGCCAACGAGTTTAATAAACTGCCAGCAAGTGCAAACTGGG
TGAATACAGCCTTTATGTTAACCTTCTCCATTGGAACAGCTCTATATGGAAAGCTATCAGACCAGCTGGGCATCAAAAAT
CTGCTTCTATTTGGGATTATGGTAAATGGCTTAGGGTCGATCATTGGATTTGTTGGACACTCTTTCTTTCCTATTCTCAT
TCTAGCCCGATTTATTCAAGGAATTGGTGCAGCCGCATTCCCAGCTCTTGTGATGGTTGTCGTTGCGCGCTATATTCCAA
AAGAGAACAGGGGGAAAGCATTTGGCCTGATCGGGTCCCTGGTAGCAATGGGAGAAGGTGTTGGGCCAGCTATTGGCGGA
ATGGTTGCTCATTATATCCATTGGTCGTATTTGCTGCTTATTCCAACTGCAACAATTATCACCGTTCCATTCCTTATAAA
ATTGTTGAAAAAGGAAGAGAGAATAAGAGGACACATTGATATGGCAGGGATTATATTGATGTCTGCAGGTATCGTATTTT
TTATGCTGTTTACAACATCTTATAGATTTTCTTTTCTGATCATCAGTATCCTTGCTTTCTTCATATTTGTGCAACACATT
AGGAAAGCTCAGGACCCTTTTGTTGACCCTGAATTAGGGAAAAACGTCTTTTTTGTGATTGGAACCCTTTGCGGAGGACT
AATATTTGGTACAGTAGCAGGATTTGTCTCTATGGTTCCTTACATGATGAAAGATGTTCACCATTTAAGTACTGCAGCAA
TTGGAAGCGGCATTATTTTCCCCGGAACAATGAGTGTCATCATCTTTGGTTACATTGGGGGATTGCTTGTTGATCGGAAA
GGTTCCTTGTATGTACTAACTATTGGAAGTGCATTGCTTTCTTCCGGCTTTTTAATCGCTGCCTTTTTTATAGATGCAGC
ACCATGGATCATGACAATTATAGTAATTTTTGTTTTTGGGGGGCTATCTTTCACAAAAACAGTTATATCTACAGTTGTGT
CTAGCAGTTTGAAAGAAAAGGAAGCGGGCGCTGGAATGAGTTTGCTTAATTTCACGAGCTTTTTATCAGAGGGAACGGGT
ATTGCGATTGTAGGCGGTTTATTATCTATCGGCTTCCTAGATCATAGGCTGCTGCCTATTGACGTTGATCACTCTACCTA
TTTGTATAGCAATATGCTCATACTTTTTGCAGGAATCATTGTTATTTGTTGGCTAGTAATCTTGAATGTATATAAACGTT
CGCGGAGACATGGCTAA

Upstream 100 bases:

>100_bases
TATTATGAAGTGCAATAAAATGAACAGGGTACAATTAAAAGAGGGAAGCGTATCAATGACCCTATAAACTGCGTCTGCCC
TCATTAATGGAGGGGAAATT

Downstream 100 bases:

>100_bases
ATGAGTGAAAGGATCAATCTTGTGAAAGTAATTCAAGGTTGATCCTTTTTTGTAAAATGAATAAACATGGCATCAATAGC
ATGTTCTTTAGATTAGCCAT

Product: multifunctional tetracycline-metal/H+ antiporter and Na+(K+)/H+ antiporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MNTSYSQSTLRHNQVLIWLCVLSFFSVLNEMVLNVSLPDIANEFNKLPASANWVNTAFMLTFSIGTALYGKLSDQLGIKN
LLLFGIMVNGLGSIIGFVGHSFFPILILARFIQGIGAAAFPALVMVVVARYIPKENRGKAFGLIGSLVAMGEGVGPAIGG
MVAHYIHWSYLLLIPTATIITVPFLIKLLKKEERIRGHIDMAGIILMSAGIVFFMLFTTSYRFSFLIISILAFFIFVQHI
RKAQDPFVDPELGKNVFFVIGTLCGGLIFGTVAGFVSMVPYMMKDVHHLSTAAIGSGIIFPGTMSVIIFGYIGGLLVDRK
GSLYVLTIGSALLSSGFLIAAFFIDAAPWIMTIIVIFVFGGLSFTKTVISTVVSSSLKEKEAGAGMSLLNFTSFLSEGTG
IAIVGGLLSIGFLDHRLLPIDVDHSTYLYSNMLILFAGIIVICWLVILNVYKRSRRHG

Sequences:

>Translated_458_residues
MNTSYSQSTLRHNQVLIWLCVLSFFSVLNEMVLNVSLPDIANEFNKLPASANWVNTAFMLTFSIGTALYGKLSDQLGIKN
LLLFGIMVNGLGSIIGFVGHSFFPILILARFIQGIGAAAFPALVMVVVARYIPKENRGKAFGLIGSLVAMGEGVGPAIGG
MVAHYIHWSYLLLIPTATIITVPFLIKLLKKEERIRGHIDMAGIILMSAGIVFFMLFTTSYRFSFLIISILAFFIFVQHI
RKAQDPFVDPELGKNVFFVIGTLCGGLIFGTVAGFVSMVPYMMKDVHHLSTAAIGSGIIFPGTMSVIIFGYIGGLLVDRK
GSLYVLTIGSALLSSGFLIAAFFIDAAPWIMTIIVIFVFGGLSFTKTVISTVVSSSLKEKEAGAGMSLLNFTSFLSEGTG
IAIVGGLLSIGFLDHRLLPIDVDHSTYLYSNMLILFAGIIVICWLVILNVYKRSRRHG
>Mature_458_residues
MNTSYSQSTLRHNQVLIWLCVLSFFSVLNEMVLNVSLPDIANEFNKLPASANWVNTAFMLTFSIGTALYGKLSDQLGIKN
LLLFGIMVNGLGSIIGFVGHSFFPILILARFIQGIGAAAFPALVMVVVARYIPKENRGKAFGLIGSLVAMGEGVGPAIGG
MVAHYIHWSYLLLIPTATIITVPFLIKLLKKEERIRGHIDMAGIILMSAGIVFFMLFTTSYRFSFLIISILAFFIFVQHI
RKAQDPFVDPELGKNVFFVIGTLCGGLIFGTVAGFVSMVPYMMKDVHHLSTAAIGSGIIFPGTMSVIIFGYIGGLLVDRK
GSLYVLTIGSALLSSGFLIAAFFIDAAPWIMTIIVIFVFGGLSFTKTVISTVVSSSLKEKEAGAGMSLLNFTSFLSEGTG
IAIVGGLLSIGFLDHRLLPIDVDHSTYLYSNMLILFAGIIVICWLVILNVYKRSRRHG

Specific function: Resistance to tetracycline by an active tetracycline efflux. This is an energy-dependent process that decreases the accumulation of the antibiotic in whole cells. This protein functions as a metal-tetracycline/H(+) antiporter

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family

Homologues:

Organism=Escherichia coli, GI1790195, Length=421, Percent_Identity=24.4655581947743, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI87081983, Length=401, Percent_Identity=22.4438902743142, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789042, Length=324, Percent_Identity=25.6172839506173, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788710, Length=317, Percent_Identity=24.2902208201893, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI48994889, Length=249, Percent_Identity=26.5060240963855, Blast_Score=62, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323735, Length=299, Percent_Identity=26.0869565217391, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TCRB_BACSU (P23054)

Other databases:

- EMBL:   X08034
- EMBL:   D26185
- EMBL:   AL009126
- PIR:   JT0427
- RefSeq:   NP_391957.1
- ProteinModelPortal:   P23054
- EnsemblBacteria:   EBBACT00000000163
- GeneID:   937890
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU40770
- NMPDR:   fig|224308.1.peg.4083
- GenoList:   BSU40770
- GeneTree:   EBGT00050000000114
- HOGENOM:   HBG349047
- OMA:   IFIAFGE
- ProtClustDB:   CLSK861686
- BioCyc:   BSUB:BSU40770-MONOMER
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411
- PRINTS:   PR01036

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 49757; Mature: 49757

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1334d86c)-; HASH(0x12805598)-; HASH(0x12f6385c)-; HASH(0x13a0ab58)-; HASH(0xfaa6eac)-; HASH(0x131ca890)-; HASH(0x13811310)-; HASH(0x132ff28c)-; HASH(0xe3cc650)-; HASH(0x135cab5c)-; HASH(0x139fd084)-; HASH(0x1317269c)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSYSQSTLRHNQVLIWLCVLSFFSVLNEMVLNVSLPDIANEFNKLPASANWVNTAFML
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHH
TFSIGTALYGKLSDQLGIKNLLLFGIMVNGLGSIIGFVGHSFFPILILARFIQGIGAAAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
PALVMVVVARYIPKENRGKAFGLIGSLVAMGEGVGPAIGGMVAHYIHWSYLLLIPTATII
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TVPFLIKLLKKEERIRGHIDMAGIILMSAGIVFFMLFTTSYRFSFLIISILAFFIFVQHI
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKAQDPFVDPELGKNVFFVIGTLCGGLIFGTVAGFVSMVPYMMKDVHHLSTAAIGSGIIF
HHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PGTMSVIIFGYIGGLLVDRKGSLYVLTIGSALLSSGFLIAAFFIDAAPWIMTIIVIFVFG
CHHHHHHHHHHHHHHEEECCCCEEEEEECHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHC
GLSFTKTVISTVVSSSLKEKEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIGFLDHRLLPI
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEE
DVDHSTYLYSNMLILFAGIIVICWLVILNVYKRSRRHG
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
>Mature Secondary Structure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ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2844262; 7584024; 9384377