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The map label for this gene is rocR

Identifier: 16081087

GI number: 16081087

Start: 4145747

End: 4147132

Strand: Direct

Name: rocR

Synonym: BSU40350

Alternate gene names: 16081087

Gene position: 4145747-4147132 (Clockwise)

Preceding gene: 16081083

Following gene: 16081097

Centisome position: 98.34

GC content: 47.04

Gene sequence:

>1386_bases
GTGGTCAAAGACAGCGAATTCCTCACATTGGTTTTTCAGAGTATTTTAGATGAAATCGATGTTGGCCTGCACGTGGTAGA
CGAGCATGGAAACACAATCGTTTATAACAATAAAATGATGCAGATTGAAGATATGGAGAAGCATGACGTCTTGAATAAAA
ACCTGATGGATGTGTTTATGTTTTCAAAGCAGCAGGACAGCACACTGGTACAGGCTCTTCAGGAAGGCAAGACGATTAAA
AACGTCAAACAGAGCTATTTCAATAATAAAGGCCAGGAAATTACGACGATCAATCATACATATCCCATCGTCCAAGACGG
CAAAATCAGGGGCGCCGTTGAAATTGCCAAGGATGTGACAAAGCTGGAACGGCTGATCAGGGAGAATATGAACAAAAAAG
GCAGCACAACCTATACGTTTGACAGTATTCTCGGCACCAGTCCGGCCATTCAAGACGTCATCGAAAACGCCAAGCGGGCA
ACCCGTACGTCATCATCTGTCCTTCTAGCAGGCGAAACCGGCACGGGAAAAGAACTGTTCGCGCAAAGTATCCATAATGG
AAGCGATCGTTCTGGCGGCCCGTTTATTTCGCAAAACTGCGCGGCACTGCCAGACAGCCTTGTGGAAAGCATTTTGTTTG
GGACGAAAAAAGGCGCGTTTACCGGCGCTGTCGACCAGCCCGGCCTGTTTGAACAAGCCCACGGCGGCACACTGCTGTTA
GATGAAATCAACTCGCTGAATCTCAGCCTTCAGGCGAAGCTGCTGCGCGCCCTTCAAGAACGGAAAATCAGGCGCATCGG
TTCAACAAAGGATACACCGATTGACGTACGTATTATTGCCACGATGAATGAAGACCCAATTGATGCAATTGCAGGCGAAC
GAATGCGGAAGGACTTGTATTACAGGCTGAGTGTCGTCACACTGATCATCCCGCCGCTTCGGGAACGAAAAGAAGACATT
TTGCTTCTCGCGTCGGAGTTCATCCAGAAAAACAACCATCTGTTCCAAATGAATGTTGAACACATCAGCGATGACGTGAA
GCAATTTTTCCTTTCTTATGACTGGCCGGGAAATATTCGCGAGCTTGAGCATATGATCGAAGGCGCAATGAATTTCATGA
CGGACGAGCAAACGATTACGGCTTCCCATCTGCCCTATCAATACCGCATGAAAATCAAGCCGGCTGACATCCCAGAGCCG
GAAACACCAAGACACCAGCCAGCCGCGGACTTAAAAGAAAAAATGGAAAGCTTCGAAAAATATGTGATAGAGAACGTGTT
AAGAAAACACGGCCATAACATCTCGAAAGCTGCTCAGGAGCTTGGCATCAGCAGACAAAGCCTCCAATATCGGCTGAAGA
AATTTTCTCACTCGTCTAACGAATAA

Upstream 100 bases:

>100_bases
AGCGAAAATTTCAGCAAGTGCAAAATTCTTTTGCATATCCTCTCCGTTTTTTTATAAAATAGAAGCAATATTAAGAAAAT
AATTGAGGGAGGTTCATACA

Downstream 100 bases:

>100_bases
TCCGGAGCAGGAAGCCTGATCAGAAAAACAGTGCCTCTGCCGATTTCACTTTTCACTTCAATCGTCCCATTGTGATTGTC
GATAATCGATTTCGTTATGG

Product: NtrC/NifA family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MVKDSEFLTLVFQSILDEIDVGLHVVDEHGNTIVYNNKMMQIEDMEKHDVLNKNLMDVFMFSKQQDSTLVQALQEGKTIK
NVKQSYFNNKGQEITTINHTYPIVQDGKIRGAVEIAKDVTKLERLIRENMNKKGSTTYTFDSILGTSPAIQDVIENAKRA
TRTSSSVLLAGETGTGKELFAQSIHNGSDRSGGPFISQNCAALPDSLVESILFGTKKGAFTGAVDQPGLFEQAHGGTLLL
DEINSLNLSLQAKLLRALQERKIRRIGSTKDTPIDVRIIATMNEDPIDAIAGERMRKDLYYRLSVVTLIIPPLRERKEDI
LLLASEFIQKNNHLFQMNVEHISDDVKQFFLSYDWPGNIRELEHMIEGAMNFMTDEQTITASHLPYQYRMKIKPADIPEP
ETPRHQPAADLKEKMESFEKYVIENVLRKHGHNISKAAQELGISRQSLQYRLKKFSHSSNE

Sequences:

>Translated_461_residues
MVKDSEFLTLVFQSILDEIDVGLHVVDEHGNTIVYNNKMMQIEDMEKHDVLNKNLMDVFMFSKQQDSTLVQALQEGKTIK
NVKQSYFNNKGQEITTINHTYPIVQDGKIRGAVEIAKDVTKLERLIRENMNKKGSTTYTFDSILGTSPAIQDVIENAKRA
TRTSSSVLLAGETGTGKELFAQSIHNGSDRSGGPFISQNCAALPDSLVESILFGTKKGAFTGAVDQPGLFEQAHGGTLLL
DEINSLNLSLQAKLLRALQERKIRRIGSTKDTPIDVRIIATMNEDPIDAIAGERMRKDLYYRLSVVTLIIPPLRERKEDI
LLLASEFIQKNNHLFQMNVEHISDDVKQFFLSYDWPGNIRELEHMIEGAMNFMTDEQTITASHLPYQYRMKIKPADIPEP
ETPRHQPAADLKEKMESFEKYVIENVLRKHGHNISKAAQELGISRQSLQYRLKKFSHSSNE
>Mature_461_residues
MVKDSEFLTLVFQSILDEIDVGLHVVDEHGNTIVYNNKMMQIEDMEKHDVLNKNLMDVFMFSKQQDSTLVQALQEGKTIK
NVKQSYFNNKGQEITTINHTYPIVQDGKIRGAVEIAKDVTKLERLIRENMNKKGSTTYTFDSILGTSPAIQDVIENAKRA
TRTSSSVLLAGETGTGKELFAQSIHNGSDRSGGPFISQNCAALPDSLVESILFGTKKGAFTGAVDQPGLFEQAHGGTLLL
DEINSLNLSLQAKLLRALQERKIRRIGSTKDTPIDVRIIATMNEDPIDAIAGERMRKDLYYRLSVVTLIIPPLRERKEDI
LLLASEFIQKNNHLFQMNVEHISDDVKQFFLSYDWPGNIRELEHMIEGAMNFMTDEQTITASHLPYQYRMKIKPADIPEP
ETPRHQPAADLKEKMESFEKYVIENVLRKHGHNISKAAQELGISRQSLQYRLKKFSHSSNE

Specific function: Positive regulator of arginine catabolism. Controls the transcription of the two operons rocABC and rocDEF and probably acts by binding to the corresponding upstream activating sequences

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain

Homologues:

Organism=Escherichia coli, GI1788550, Length=346, Percent_Identity=43.6416184971098, Blast_Score=262, Evalue=3e-71,
Organism=Escherichia coli, GI1790437, Length=313, Percent_Identity=41.5335463258786, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1789087, Length=445, Percent_Identity=35.9550561797753, Blast_Score=221, Evalue=9e-59,
Organism=Escherichia coli, GI87082117, Length=367, Percent_Identity=37.3297002724796, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1788905, Length=228, Percent_Identity=48.2456140350877, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI87082152, Length=348, Percent_Identity=37.3563218390805, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1786524, Length=369, Percent_Identity=36.3143631436314, Blast_Score=208, Evalue=7e-55,
Organism=Escherichia coli, GI1789233, Length=320, Percent_Identity=36.875, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI1790299, Length=348, Percent_Identity=35.9195402298851, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI1787583, Length=357, Percent_Identity=30.812324929972, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI87081872, Length=322, Percent_Identity=35.0931677018634, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI87081858, Length=444, Percent_Identity=27.4774774774775, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1789828, Length=243, Percent_Identity=32.5102880658436, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ROCR_BACSU (P38022)

Other databases:

- EMBL:   L22006
- EMBL:   D78193
- EMBL:   AL009126
- PIR:   A53370
- RefSeq:   NP_391915.1
- ProteinModelPortal:   P38022
- SMR:   P38022
- EnsemblBacteria:   EBBACT00000003185
- GeneID:   937769
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU40350
- NMPDR:   fig|224308.1.peg.4041
- GenoList:   BSU40350
- GeneTree:   EBGT00070000031868
- HOGENOM:   HBG740884
- OMA:   IATMNED
- PhylomeDB:   P38022
- ProtClustDB:   CLSK873761
- BioCyc:   BSUB:BSU40350-MONOMER
- GO:   GO:0005622
- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR002078
- PRINTS:   PR01590
- SMART:   SM00382
- SMART:   SM00091
- TIGRFAMs:   TIGR00229

Pfam domain/function: PF02954 HTH_8; PF08448 PAS_4; PF00158 Sigma54_activat; SSF46689 Homeodomain_like

EC number: NA

Molecular weight: Translated: 52178; Mature: 52178

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50112 PAS; PS00675 SIGMA54_INTERACT_1; PS00676 SIGMA54_INTERACT_2; PS00688 SIGMA54_INTERACT_3; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKDSEFLTLVFQSILDEIDVGLHVVDEHGNTIVYNNKMMQIEDMEKHDVLNKNLMDVFM
CCCCHHHHHHHHHHHHHHHCCCEEEEECCCCEEEECCEEEEECCCHHHHHHHHHHHHHHH
FSKQQDSTLVQALQEGKTIKNVKQSYFNNKGQEITTINHTYPIVQDGKIRGAVEIAKDVT
HCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEECCEECCEECCCCCHHHHHHHHHH
KLERLIRENMNKKGSTTYTFDSILGTSPAIQDVIENAKRATRTSSSVLLAGETGTGKELF
HHHHHHHHHCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHH
AQSIHNGSDRSGGPFISQNCAALPDSLVESILFGTKKGAFTGAVDQPGLFEQAHGGTLLL
HHHHCCCCCCCCCCCCCCCHHCCCHHHHHHHHHCCCCCCEECCCCCCCCHHHCCCCEEEE
DEINSLNLSLQAKLLRALQERKIRRIGSTKDTPIDVRIIATMNEDPIDAIAGERMRKDLY
ECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
YRLSVVTLIIPPLRERKEDILLLASEFIQKNNHLFQMNVEHISDDVKQFFLSYDWPGNIR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCCCHH
ELEHMIEGAMNFMTDEQTITASHLPYQYRMKIKPADIPEPETPRHQPAADLKEKMESFEK
HHHHHHHHHHHHHCCCCEEECCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
YVIENVLRKHGHNISKAAQELGISRQSLQYRLKKFSHSSNE
HHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MVKDSEFLTLVFQSILDEIDVGLHVVDEHGNTIVYNNKMMQIEDMEKHDVLNKNLMDVFM
CCCCHHHHHHHHHHHHHHHCCCEEEEECCCCEEEECCEEEEECCCHHHHHHHHHHHHHHH
FSKQQDSTLVQALQEGKTIKNVKQSYFNNKGQEITTINHTYPIVQDGKIRGAVEIAKDVT
HCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEECCEECCEECCCCCHHHHHHHHHH
KLERLIRENMNKKGSTTYTFDSILGTSPAIQDVIENAKRATRTSSSVLLAGETGTGKELF
HHHHHHHHHCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHH
AQSIHNGSDRSGGPFISQNCAALPDSLVESILFGTKKGAFTGAVDQPGLFEQAHGGTLLL
HHHHCCCCCCCCCCCCCCCHHCCCHHHHHHHHHCCCCCCEECCCCCCCCHHHCCCCEEEE
DEINSLNLSLQAKLLRALQERKIRRIGSTKDTPIDVRIIATMNEDPIDAIAGERMRKDLY
ECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
YRLSVVTLIIPPLRERKEDILLLASEFIQKNNHLFQMNVEHISDDVKQFFLSYDWPGNIR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCCCHH
ELEHMIEGAMNFMTDEQTITASHLPYQYRMKIKPADIPEPETPRHQPAADLKEKMESFEK
HHHHHHHHHHHHHCCCCEEECCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
YVIENVLRKHGHNISKAAQELGISRQSLQYRLKKFSHSSNE
HHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113162; 9384377