Click here to switch to the map view.

The map label for this gene is iolJ

Identifier: 16081018

GI number: 16081018

Start: 4073081

End: 4073953

Strand: Reverse

Name: iolJ

Synonym: BSU39670

Alternate gene names: 16081018

Gene position: 4073953-4073081 (Counterclockwise)

Preceding gene: 16081019

Following gene: 16081017

Centisome position: 96.64

GC content: 53.61

Gene sequence:

>873_bases
ATGGCTTTTGTATCGATGAAAGAGCTTCTTGAAGATGCAAAGCGGGAGCAATATGCAATTGGCCAGTTTAATATCAACGG
CCTGCAATGGACGAAGGCGATTTTGCAGGCGGCGCAAAAGGAGCAATCACCGGTCATCGCCGCGGCTTCCGATCGCCTGG
TCGACTATTTAGGCGGATTTAAAACGATTGCCGCCATGGTCGGCGCGTTAATAGAGGACATGGCGATTACCGTTCCGGTC
GTGCTTCATCTCGATCACGGCAGCAGTGCGGAACGCTGCAGACAGGCCATTGATGCCGGATTCAGCTCAGTGATGATTGA
CGGCTCCCATCAGCCGATTGACGAGAATATCGCGATGACAAAAGAAGTCACCGATTATGCCGCAAAACACGGCGTGTCAG
TAGAAGCCGAAGTCGGCACGGTCGGCGGAATGGAAGACGGACTGGTCGGCGGGGTCCGCTATGCGGATATCACGGAATGT
GAGCGGATCGTTAAAGAAACCAATATCGACGCGCTGGCCGCCGCCCTCGGCTCTGTACACGGCAAATATCAGGGTGAGCC
GAATCTCGGATTTAAGGAAATGGAGGCTATCTCCCGCATGACTGATATTCCCCTCGTTCTTCACGGGGCATCCGGGATTC
CGCAGGATCAGATCAAAAAAGCCATCACGCTCGGCCACGCGAAGATCAATATCAATACGGAATGTATGGTAGCGTGGACA
GACGAAACACGCCGCATGTTTCAGGAAAACAGCGATCTGTACGAACCGCGCGGCTATTTGACACCCGGCATTGAAGCCGT
GGAAGAGACAGTGCGAAGCAAAATGAGAGAGTTCGGATCAGCCGGTAAAGCAGCTAAGCAGCAGGTCGGCTAA

Upstream 100 bases:

>100_bases
ATAAGCTGACTGCTGAGGAAGCCATTCAAACAGCGAAAAAAACAACAGTGGATGTCGTATCAAAATACTTCAGCATGTAA
TGGATAAAGGAGGGGTGAAT

Downstream 100 bases:

>100_bases
AGAGACCAGAAACCGCCGAGATGGCGGTTTTTTTGTGCGGTGTTGCGTCCATACGCCCAAAACGATTATGATGATGGCAG
CAGTGTAAGAAACGGGAGTG

Product: 2-deoxy-5-keto-D-gluconic acid 6-phosphate aldolase

Products: NA

Alternate protein names: DKGP aldolase [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MAFVSMKELLEDAKREQYAIGQFNINGLQWTKAILQAAQKEQSPVIAAASDRLVDYLGGFKTIAAMVGALIEDMAITVPV
VLHLDHGSSAERCRQAIDAGFSSVMIDGSHQPIDENIAMTKEVTDYAAKHGVSVEAEVGTVGGMEDGLVGGVRYADITEC
ERIVKETNIDALAAALGSVHGKYQGEPNLGFKEMEAISRMTDIPLVLHGASGIPQDQIKKAITLGHAKININTECMVAWT
DETRRMFQENSDLYEPRGYLTPGIEAVEETVRSKMREFGSAGKAAKQQVG

Sequences:

>Translated_290_residues
MAFVSMKELLEDAKREQYAIGQFNINGLQWTKAILQAAQKEQSPVIAAASDRLVDYLGGFKTIAAMVGALIEDMAITVPV
VLHLDHGSSAERCRQAIDAGFSSVMIDGSHQPIDENIAMTKEVTDYAAKHGVSVEAEVGTVGGMEDGLVGGVRYADITEC
ERIVKETNIDALAAALGSVHGKYQGEPNLGFKEMEAISRMTDIPLVLHGASGIPQDQIKKAITLGHAKININTECMVAWT
DETRRMFQENSDLYEPRGYLTPGIEAVEETVRSKMREFGSAGKAAKQQVG
>Mature_289_residues
AFVSMKELLEDAKREQYAIGQFNINGLQWTKAILQAAQKEQSPVIAAASDRLVDYLGGFKTIAAMVGALIEDMAITVPVV
LHLDHGSSAERCRQAIDAGFSSVMIDGSHQPIDENIAMTKEVTDYAAKHGVSVEAEVGTVGGMEDGLVGGVRYADITECE
RIVKETNIDALAAALGSVHGKYQGEPNLGFKEMEAISRMTDIPLVLHGASGIPQDQIKKAITLGHAKININTECMVAWTD
ETRRMFQENSDLYEPRGYLTPGIEAVEETVRSKMREFGSAGKAAKQQVG

Specific function: Produces dihydroxyacetone phosphate (DHAP or glycerone phosphate) and malonic semialdehyde (MSA or 3-oxopropanoate) from 6-phospho-5-dehydro-2-deoxy-D-gluconate (DKGP) [H]

COG id: COG0191

COG function: function code G; Fructose/tagatose bisphosphate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class II fructose-bisphosphate aldolase family. IolJ subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789526, Length=289, Percent_Identity=38.4083044982699, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI87082041, Length=287, Percent_Identity=34.4947735191638, Blast_Score=178, Evalue=4e-46,
Organism=Escherichia coli, GI1788072, Length=278, Percent_Identity=38.1294964028777, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1789293, Length=327, Percent_Identity=27.5229357798165, Blast_Score=90, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322790, Length=281, Percent_Identity=27.7580071174377, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011289
- InterPro:   IPR000771 [H]

Pfam domain/function: PF01116 F_bP_aldolase [H]

EC number: =4.1.2.29 [H]

Molecular weight: Translated: 31372; Mature: 31241

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00602 ALDOLASE_CLASS_II_1 ; PS00806 ALDOLASE_CLASS_II_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFVSMKELLEDAKREQYAIGQFNINGLQWTKAILQAAQKEQSPVIAAASDRLVDYLGGF
CCCHHHHHHHHHHHHHHEECCEEECCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHH
KTIAAMVGALIEDMAITVPVVLHLDHGSSAERCRQAIDAGFSSVMIDGSHQPIDENIAMT
HHHHHHHHHHHHHHHEEEEEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCHHHH
KEVTDYAAKHGVSVEAEVGTVGGMEDGLVGGVRYADITECERIVKETNIDALAAALGSVH
HHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHC
GKYQGEPNLGFKEMEAISRMTDIPLVLHGASGIPQDQIKKAITLGHAKININTECMVAWT
CCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCEEEEECCEEEEEEC
DETRRMFQENSDLYEPRGYLTPGIEAVEETVRSKMREFGSAGKAAKQQVG
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCC
>Mature Secondary Structure 
AFVSMKELLEDAKREQYAIGQFNINGLQWTKAILQAAQKEQSPVIAAASDRLVDYLGGF
CCHHHHHHHHHHHHHHEECCEEECCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHH
KTIAAMVGALIEDMAITVPVVLHLDHGSSAERCRQAIDAGFSSVMIDGSHQPIDENIAMT
HHHHHHHHHHHHHHHEEEEEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCHHHH
KEVTDYAAKHGVSVEAEVGTVGGMEDGLVGGVRYADITECERIVKETNIDALAAALGSVH
HHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHC
GKYQGEPNLGFKEMEAISRMTDIPLVLHGASGIPQDQIKKAITLGHAKININTECMVAWT
CCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCEEEEECCEEEEEEC
DETRRMFQENSDLYEPRGYLTPGIEAVEETVRSKMREFGSAGKAAKQQVG
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA