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The map label for this gene is licH

Identifier: 16080907

GI number: 16080907

Start: 3958516

End: 3959844

Strand: Reverse

Name: licH

Synonym: BSU38560

Alternate gene names: 16080907

Gene position: 3959844-3958516 (Counterclockwise)

Preceding gene: 16080908

Following gene: 16080900

Centisome position: 93.93

GC content: 49.51

Gene sequence:

>1329_bases
ATGACAAAAGGATTGAAGATTGTAACGATTGGCGGAGGTTCAAGCTATACGCCCGAGCTAGTGGAGGGGTTCATTAAACG
GTATGATGAGCTGCCTGTCCGTGAATTATGGCTTGTCGATATCCCCGAGGGCGAAGAAAAGCTGAATATTGTCGGCACAC
TCGCCAAACGGATGGTTGAAAAAGCTGGCGTCCCGATTGACATTCATCTTACGCTCGACCGGAGAAAGGCGCTGAAAGAC
GCAGACTTTGTCACGACCCAATTCCGCGTCGGACTTTTGCAGGCAAGAGCAAAGGATGAGCGCATTCCGCTAAAATACGG
AGTGATAGGCCAGGAAACAAACGGCCCGGGAGGTCTGTTTAAAGGATTGCGCACAATTCCGGTCATCCTTGAGATCGCAA
AGGACATAGAAGAGCTTTGCCCGAACGCTTGGCTTGTTAACTTCACAAACCCTGCCGGCATGGTAACAGAAGCCTTGCTT
CGCTACTCCAACCTGAAGAAAGTCGTCGGTCTTTGCAATGTTCCAATCGGCATCAAAATGGGCGTAGCCAAAGCGCTTGA
TGTAGATGTGGACCGCGTAGAAGTCCAATTTGCCGGACTGAACCATATGGTATTCGGGCTGGATGTTTTCTTGGACGGCG
TAAGTGTGAAGGAGCAAGTCATCGAGGCGATGGGCGACCCGAAAAACGCGATGACGATGAAAAATATCTCAGGCGCCGAA
TGGGAACCGGATTTCTTAAAAGCGCTCAATGTCATCCCTTGCGGCTATCACCGTTACTATTTTAAAACAAAAGAGATGCT
GGAGCACGAACTGGAAGCATCACAAACGGAGGGCACCCGCGCTGAAGTCGTACAGAAGGTCGAAAAAGAGCTGTTCGAGC
TTTATAAAGATCCAAACCTTGCGATCAAACCGCCGCAGCTGGAAAAACGGGGCGGAGCTTACTACAGTGATGCAGCATGT
AACTTAATCAGCTCCATTTATAATGATAAACATGACATTCAGCCGGTGAACACGATCAATAATGGTGCAATAGCGAGTAT
TCCAGACGATTCCGCAGTTGAAGTGAACTGTGTGATGACGAAAACCGGCCCTAAGCCAATTGCTGTCGGCGACTTGCCGG
TGTCTGTCCGCGGCCTTGTCCAGCAAATCAAATCCTTTGAACGTGTCGCGGCAGAAGCGGCAGTTACAGGTGACTATCAA
ACTGCGCTCCTTGCCATGACGATTAACCCGCTTGTCCCGTCCGATACTGTGGCCAAACAGATTTTAGACGAAATGCTAGA
GGCGCACAAAGCGTATCTGCCGCAGTTTTTCAACAAAATTGAAGCGTAA

Upstream 100 bases:

>100_bases
ATGCACAGGATCATTTAATGAATGCAATGACAGTGAAGGATATGGCTGCAGAGATCATTGAGTTATATGAAAAAATCACC
GAGCAAAGGGGAGCAAGCAT

Downstream 100 bases:

>100_bases
AAAAAGCCGGCCTGTAATGGGCCGGCTTTTTTCTTACTTGCTTTCAGTCAGCGCTGCGACTTCGGTCGTCCATCCGAATT
CGTCTGCGACAGCGCCTTTT

Product: 6-phospho-beta-glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MTKGLKIVTIGGGSSYTPELVEGFIKRYDELPVRELWLVDIPEGEEKLNIVGTLAKRMVEKAGVPIDIHLTLDRRKALKD
ADFVTTQFRVGLLQARAKDERIPLKYGVIGQETNGPGGLFKGLRTIPVILEIAKDIEELCPNAWLVNFTNPAGMVTEALL
RYSNLKKVVGLCNVPIGIKMGVAKALDVDVDRVEVQFAGLNHMVFGLDVFLDGVSVKEQVIEAMGDPKNAMTMKNISGAE
WEPDFLKALNVIPCGYHRYYFKTKEMLEHELEASQTEGTRAEVVQKVEKELFELYKDPNLAIKPPQLEKRGGAYYSDAAC
NLISSIYNDKHDIQPVNTINNGAIASIPDDSAVEVNCVMTKTGPKPIAVGDLPVSVRGLVQQIKSFERVAAEAAVTGDYQ
TALLAMTINPLVPSDTVAKQILDEMLEAHKAYLPQFFNKIEA

Sequences:

>Translated_442_residues
MTKGLKIVTIGGGSSYTPELVEGFIKRYDELPVRELWLVDIPEGEEKLNIVGTLAKRMVEKAGVPIDIHLTLDRRKALKD
ADFVTTQFRVGLLQARAKDERIPLKYGVIGQETNGPGGLFKGLRTIPVILEIAKDIEELCPNAWLVNFTNPAGMVTEALL
RYSNLKKVVGLCNVPIGIKMGVAKALDVDVDRVEVQFAGLNHMVFGLDVFLDGVSVKEQVIEAMGDPKNAMTMKNISGAE
WEPDFLKALNVIPCGYHRYYFKTKEMLEHELEASQTEGTRAEVVQKVEKELFELYKDPNLAIKPPQLEKRGGAYYSDAAC
NLISSIYNDKHDIQPVNTINNGAIASIPDDSAVEVNCVMTKTGPKPIAVGDLPVSVRGLVQQIKSFERVAAEAAVTGDYQ
TALLAMTINPLVPSDTVAKQILDEMLEAHKAYLPQFFNKIEA
>Mature_441_residues
TKGLKIVTIGGGSSYTPELVEGFIKRYDELPVRELWLVDIPEGEEKLNIVGTLAKRMVEKAGVPIDIHLTLDRRKALKDA
DFVTTQFRVGLLQARAKDERIPLKYGVIGQETNGPGGLFKGLRTIPVILEIAKDIEELCPNAWLVNFTNPAGMVTEALLR
YSNLKKVVGLCNVPIGIKMGVAKALDVDVDRVEVQFAGLNHMVFGLDVFLDGVSVKEQVIEAMGDPKNAMTMKNISGAEW
EPDFLKALNVIPCGYHRYYFKTKEMLEHELEASQTEGTRAEVVQKVEKELFELYKDPNLAIKPPQLEKRGGAYYSDAACN
LISSIYNDKHDIQPVNTINNGAIASIPDDSAVEVNCVMTKTGPKPIAVGDLPVSVRGLVQQIKSFERVAAEAAVTGDYQT
ALLAMTINPLVPSDTVAKQILDEMLEAHKAYLPQFFNKIEA

Specific function: Hydrolyzes phospho-beta-glucosides (Probable)

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family

Homologues:

Organism=Escherichia coli, GI1788029, Length=443, Percent_Identity=53.4988713318284, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI1790560, Length=478, Percent_Identity=27.4058577405858, Blast_Score=138, Evalue=9e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LICH_BACSU (P46320)

Other databases:

- EMBL:   Z49992
- EMBL:   AL009126
- EMBL:   D83026
- PIR:   S57762
- RefSeq:   NP_391735.1
- ProteinModelPortal:   P46320
- SMR:   P46320
- EnsemblBacteria:   EBBACT00000000446
- GeneID:   937373
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU38560
- NMPDR:   fig|224308.1.peg.3861
- GenoList:   BSU38560
- GeneTree:   EBGT00050000000927
- HOGENOM:   HBG511715
- OMA:   AKDERIP
- PhylomeDB:   P46320
- ProtClustDB:   CLSK2752558
- BioCyc:   BSUB:BSU38560-MONOMER
- BRENDA:   3.2.1.86
- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00732

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =3.2.1.86

Molecular weight: Translated: 48712; Mature: 48581

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: ACT_SITE 256-256 BINDING 96-96 BINDING 150-150

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKGLKIVTIGGGSSYTPELVEGFIKRYDELPVRELWLVDIPEGEEKLNIVGTLAKRMVE
CCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
KAGVPIDIHLTLDRRKALKDADFVTTQFRVGLLQARAKDERIPLKYGVIGQETNGPGGLF
HCCCCEEEEEEECHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEECEEECCCCCCHHHH
KGLRTIPVILEIAKDIEELCPNAWLVNFTNPAGMVTEALLRYSNLKKVVGLCNVPIGIKM
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GVAKALDVDVDRVEVQFAGLNHMVFGLDVFLDGVSVKEQVIEAMGDPKNAMTMKNISGAE
CHHHHHCCCCEEEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEECCCCCCC
WEPDFLKALNVIPCGYHRYYFKTKEMLEHELEASQTEGTRAEVVQKVEKELFELYKDPNL
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCC
AIKPPQLEKRGGAYYSDAACNLISSIYNDKHDIQPVNTINNGAIASIPDDSAVEVNCVMT
EECCCCCHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCEEEEEEEE
KTGPKPIAVGDLPVSVRGLVQQIKSFERVAAEAAVTGDYQTALLAMTINPLVPSDTVAKQ
CCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEECCCCCCHHHHHH
ILDEMLEAHKAYLPQFFNKIEA
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKGLKIVTIGGGSSYTPELVEGFIKRYDELPVRELWLVDIPEGEEKLNIVGTLAKRMVE
CCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
KAGVPIDIHLTLDRRKALKDADFVTTQFRVGLLQARAKDERIPLKYGVIGQETNGPGGLF
HCCCCEEEEEEECHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEECEEECCCCCCHHHH
KGLRTIPVILEIAKDIEELCPNAWLVNFTNPAGMVTEALLRYSNLKKVVGLCNVPIGIKM
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GVAKALDVDVDRVEVQFAGLNHMVFGLDVFLDGVSVKEQVIEAMGDPKNAMTMKNISGAE
CHHHHHCCCCEEEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEECCCCCCC
WEPDFLKALNVIPCGYHRYYFKTKEMLEHELEASQTEGTRAEVVQKVEKELFELYKDPNL
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCC
AIKPPQLEKRGGAYYSDAACNLISSIYNDKHDIQPVNTINNGAIASIPDDSAVEVNCVMT
EECCCCCHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCEEEEEEEE
KTGPKPIAVGDLPVSVRGLVQQIKSFERVAAEAAVTGDYQTALLAMTINPLVPSDTVAKQ
CCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEECCCCCCHHHHHH
ILDEMLEAHKAYLPQFFNKIEA
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8990303; 9384377; 8969509