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The map label for this gene is bacD

Identifier: 16080823

GI number: 16080823

Start: 3870668

End: 3872086

Strand: Reverse

Name: bacD

Synonym: BSU37710

Alternate gene names: 16080823

Gene position: 3872086-3870668 (Counterclockwise)

Preceding gene: 16080824

Following gene: 16080822

Centisome position: 91.85

GC content: 44.19

Gene sequence:

>1419_bases
ATGGAGAGAAAAACAGTATTGGTCATCGCTGATCTTGGAGGCTGCCCGCCGCACATGTTTTATAAAAGCGCTGCTGAAAA
ATATAACCTGGTCAGCTTTATTCCAAGACCTTTTGCAATTACAGCCTCCCATGCAGCATTGATTGAAAAATACTCGGTCG
CGGTCATAAAAGATAAAGACTATTTTAAGAGTTTAGCTGATTTTGAACACCCTGATTCCATTTATTGGGCGCATGAAGAT
CATAACAAGCCTGAGGAAGAGGTCGTCGAGCAAATCGTCAAGGTTGCCGAAATGTTTGGGGCGGATGCCATCACAACAAA
CAATGAATTATTCATTGCTCCGATGGCGAAAGCCTGTGAACGTCTGGGCTTGAGAGGTGCCGGCGTGCAGGCAGCCGAAA
ATGCCAGAGATAAAAATAAAATGAGGGACGCTTTTAATAAGGCCGGAGTCAAATCGATCAAAAACAAACGAGTCACAACT
CTTGAAGATTTCCGTGCTGCTCTTGAAGAGATCGGCACACCTCTTATCTTAAAGCCTACATACTTAGCGAGTTCTATCGG
TGTAACGCTGATTACGGACACTGAGACGGCAGAAGATGAATTTAACAGAGTCAATGACTATCTGAAATCAATTAACGTGC
CAAAGGCGGTTACGTTTGAAGCGCCGTTTATCGCTGAAGAATTTTTACAGGGTGAGTACGGAGACTGGTATCAAACAGAA
GGGTACTCCGACTATATCAGTATAGAAGGCATCATGGCTGACGGTGAGTATTTCCCGATCGCCATTCATGATAAAACGCC
GCAAATCGGGTTTACAGAGACATCCCACATTACGCCGTCCATTCTGGATGAAGAGGCAAAAAAGAAAATTGTCGAAGCTG
CCAAAAAGGCAAATGAAGGGCTTGGACTGCAAAATTGCGCAACACATACAGAGATCAAGCTAATGAAAAACAGAGAACCG
GGTTTAATAGAGTCGGCAGCCAGATTTGCCGGCTGGAATATGATCCCCAATATTAAAAAGGTCTTTGGCCTTGATATGGC
GCAATTATTATTAGATGTCCTCTGTTTCGGAAAAGACGCCGATCTGCCGGACGGATTATTGGATCAAGAGCCTTATTATG
TTGCCGACTGCCATTTGTACCCGCAGCATTTCAAACAAAATGGCCAAATTCCTGAAACTGCTGAGGATTTGGTCATTGAA
GCGATCGATATTCCGGACGGGCTTTTAAAAGGGGATACTGAAATCGTTTCTTTTTCGGCCGCAGCACCAGGCACTTCAGT
TGATTTGACATTGTTTGAAGCTTTCAATTCCATTGCTGCATTTGAACTGAAAGGCAGTAATTCACAGGATGTGGCTGAAT
CAATCAGACAAATTCAGCAGCATGCGAAGCTGACGGCAAAGTATGTGCTGCCAGTATGA

Upstream 100 bases:

>100_bases
GATGCTGTTTTTAGCCTCGGATTTATCAAGCTATATGACCGGAAGCGCCATCACCGCAGACGGAGGATACACCGCACAAT
AGAGAAGGAGTGTTTTACAT

Downstream 100 bases:

>100_bases
AACAGCTGAAACCCAACTCTAAATACTTGCTTTACGGGCAGGCGCTATCGTTCATGGGAGACTATTGTGTTCTCCCTGCC
CTGCTCATTTTGTCTACATA

Product: alanine-anticapsin ligase

Products: NA

Alternate protein names: Bacilysin synthetase; L-amino acid ligase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MERKTVLVIADLGGCPPHMFYKSAAEKYNLVSFIPRPFAITASHAALIEKYSVAVIKDKDYFKSLADFEHPDSIYWAHED
HNKPEEEVVEQIVKVAEMFGADAITTNNELFIAPMAKACERLGLRGAGVQAAENARDKNKMRDAFNKAGVKSIKNKRVTT
LEDFRAALEEIGTPLILKPTYLASSIGVTLITDTETAEDEFNRVNDYLKSINVPKAVTFEAPFIAEEFLQGEYGDWYQTE
GYSDYISIEGIMADGEYFPIAIHDKTPQIGFTETSHITPSILDEEAKKKIVEAAKKANEGLGLQNCATHTEIKLMKNREP
GLIESAARFAGWNMIPNIKKVFGLDMAQLLLDVLCFGKDADLPDGLLDQEPYYVADCHLYPQHFKQNGQIPETAEDLVIE
AIDIPDGLLKGDTEIVSFSAAAPGTSVDLTLFEAFNSIAAFELKGSNSQDVAESIRQIQQHAKLTAKYVLPV

Sequences:

>Translated_472_residues
MERKTVLVIADLGGCPPHMFYKSAAEKYNLVSFIPRPFAITASHAALIEKYSVAVIKDKDYFKSLADFEHPDSIYWAHED
HNKPEEEVVEQIVKVAEMFGADAITTNNELFIAPMAKACERLGLRGAGVQAAENARDKNKMRDAFNKAGVKSIKNKRVTT
LEDFRAALEEIGTPLILKPTYLASSIGVTLITDTETAEDEFNRVNDYLKSINVPKAVTFEAPFIAEEFLQGEYGDWYQTE
GYSDYISIEGIMADGEYFPIAIHDKTPQIGFTETSHITPSILDEEAKKKIVEAAKKANEGLGLQNCATHTEIKLMKNREP
GLIESAARFAGWNMIPNIKKVFGLDMAQLLLDVLCFGKDADLPDGLLDQEPYYVADCHLYPQHFKQNGQIPETAEDLVIE
AIDIPDGLLKGDTEIVSFSAAAPGTSVDLTLFEAFNSIAAFELKGSNSQDVAESIRQIQQHAKLTAKYVLPV
>Mature_472_residues
MERKTVLVIADLGGCPPHMFYKSAAEKYNLVSFIPRPFAITASHAALIEKYSVAVIKDKDYFKSLADFEHPDSIYWAHED
HNKPEEEVVEQIVKVAEMFGADAITTNNELFIAPMAKACERLGLRGAGVQAAENARDKNKMRDAFNKAGVKSIKNKRVTT
LEDFRAALEEIGTPLILKPTYLASSIGVTLITDTETAEDEFNRVNDYLKSINVPKAVTFEAPFIAEEFLQGEYGDWYQTE
GYSDYISIEGIMADGEYFPIAIHDKTPQIGFTETSHITPSILDEEAKKKIVEAAKKANEGLGLQNCATHTEIKLMKNREP
GLIESAARFAGWNMIPNIKKVFGLDMAQLLLDVLCFGKDADLPDGLLDQEPYYVADCHLYPQHFKQNGQIPETAEDLVIE
AIDIPDGLLKGDTEIVSFSAAAPGTSVDLTLFEAFNSIAAFELKGSNSQDVAESIRQIQQHAKLTAKYVLPV

Specific function: Catalyzes the formation of alpha-dipeptides from various L-amino acids in the presence of ATP. Probably catalyzes the ligation of L-alanine and L-anticapsin to produce the final bacilysin antibiotic [H]

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011095 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C [H]

EC number: =6.3.2.28 [H]

Molecular weight: Translated: 52268; Mature: 52268

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERKTVLVIADLGGCPPHMFYKSAAEKYNLVSFIPRPFAITASHAALIEKYSVAVIKDKD
CCCCEEEEEECCCCCCHHHHHHHHHHHCCHHHHCCCCEEEECHHHHHHHHHEEEEEECHH
YFKSLADFEHPDSIYWAHEDHNKPEEEVVEQIVKVAEMFGADAITTNNELFIAPMAKACE
HHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCEEEECHHHHHH
RLGLRGAGVQAAENARDKNKMRDAFNKAGVKSIKNKRVTTLEDFRAALEEIGTPLILKPT
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEECH
YLASSIGVTLITDTETAEDEFNRVNDYLKSINVPKAVTFEAPFIAEEFLQGEYGDWYQTE
HHHHHCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCCCCCCCCCCC
GYSDYISIEGIMADGEYFPIAIHDKTPQIGFTETSHITPSILDEEAKKKIVEAAKKANEG
CCCCEEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
LGLQNCATHTEIKLMKNREPGLIESAARFAGWNMIPNIKKVFGLDMAQLLLDVLCFGKDA
CCCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHCCCCC
DLPDGLLDQEPYYVADCHLYPQHFKQNGQIPETAEDLVIEAIDIPDGLLKGDTEIVSFSA
CCCCCCCCCCCCEEEEEEECHHHHHCCCCCCCCHHHHEEEEECCCCCHHCCCCCEEEEEC
AAPGTSVDLTLFEAFNSIAAFELKGSNSQDVAESIRQIQQHAKLTAKYVLPV
CCCCCCEEEHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHEEECCC
>Mature Secondary Structure
MERKTVLVIADLGGCPPHMFYKSAAEKYNLVSFIPRPFAITASHAALIEKYSVAVIKDKD
CCCCEEEEEECCCCCCHHHHHHHHHHHCCHHHHCCCCEEEECHHHHHHHHHEEEEEECHH
YFKSLADFEHPDSIYWAHEDHNKPEEEVVEQIVKVAEMFGADAITTNNELFIAPMAKACE
HHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCEEEECHHHHHH
RLGLRGAGVQAAENARDKNKMRDAFNKAGVKSIKNKRVTTLEDFRAALEEIGTPLILKPT
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEECH
YLASSIGVTLITDTETAEDEFNRVNDYLKSINVPKAVTFEAPFIAEEFLQGEYGDWYQTE
HHHHHCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCCCCCCCCCCC
GYSDYISIEGIMADGEYFPIAIHDKTPQIGFTETSHITPSILDEEAKKKIVEAAKKANEG
CCCCEEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
LGLQNCATHTEIKLMKNREPGLIESAARFAGWNMIPNIKKVFGLDMAQLLLDVLCFGKDA
CCCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHCCCCC
DLPDGLLDQEPYYVADCHLYPQHFKQNGQIPETAEDLVIEAIDIPDGLLKGDTEIVSFSA
CCCCCCCCCCCCEEEEEEECHHHHHCCCCCCCCHHHHEEEEECCCCCHHCCCCCEEEEEC
AAPGTSVDLTLFEAFNSIAAFELKGSNSQDVAESIRQIQQHAKLTAKYVLPV
CCCCCCEEEHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA