Click here to switch to the map view.

The map label for this gene is albF

Identifier: 16080794

GI number: 16080794

Start: 3841017

End: 3842297

Strand: Direct

Name: albF

Synonym: BSU37420

Alternate gene names: 16080794

Gene position: 3841017-3842297 (Clockwise)

Preceding gene: 16080793

Following gene: 16080795

Centisome position: 91.11

GC content: 45.98

Gene sequence:

>1281_bases
ATGGAAAAGAAAGCCTTTTTTCAACAGCTGGATGAACGAACCGATATCAGATACACAGACAGCGGACTAAAAATATTCAG
ATTGAAATTCCCCCGCGCCCACCTGCGGCTGTGCAATGTAAAAATCGATTTTGGAAGCCGGGATGTATGTATACGGGCAG
AATCCGGTGATACACTGCTTCCGTATGGCACAGCACATTTTTTAGAGCATCTTCTCTTCTGGCATAACGGCCGCAATCTG
TACAGTGATTTTTTTGCGCATGGCGCGCTTTTAAACGCATTCACTACCTACACCGATACCAATTTCATGTTTACCTCATT
ACCGGATCGGCTCAGACAAACGATTCCCATCCTGCTTGATGCTTTATGGAACCACTCATTTGACAAGAAGATTGTCGCTC
AGGAAAAAGCTGTGATTACAAGCGAAATTCAAACAGCACACCTTAACCATCAGCTCTCATATCATTATCAGCTGATCAGC
ATGCTTTCTCCCTCCTCACCAGCTGCCGTTTTCCCCGCAGGCAGAATCGAAGATATTGAGGCGCTGGACATCAGTGATTT
ACAAAAAGCCTATAAGGCTGCCTACCAAGCCCATCGGATGACGCTGTTTTTGATTGGGGGCTCAGAAAATACAGAAACGC
TTTTGCCTCCTCACCTCCAGCTGGAAAAGCGTCCGGATTATCATGCTGAAAGAAAAATCATCCCGGCATGCCCTCCAGTC
CTCTCACAAAAAATGATGCTGGGTGATGAGGAGCGGATGGAAGATACCTGGACCGGATTACAGATCGGCGCACTTCCCGG
GCAAAACGATCTGTTGTCGATAAAGCTTTATTGGGATATCGCGGCTCGTATTCTATTTCAATTAGATTCACCGTTTTTTC
AAGAGATTCAGCAAACGTACCGCCTGGAAATCGATCGCTTGTCAGCCGAGACTTATATATATGAGGATGGCGGTTTCTTG
ATCCTTCACAGTCAAGGGACCCATTCTTCAGCTTATATCGATGTGGCTTCATACTATGTCACACAGAAGAAAGAACAGGT
TGCCGCCTGGCTTCAATACGGCAAGGACTCTTTGACAGACGCGATCATTTATGACAGCGATTATGTGCGTAAATGCTTCG
AGTGGGCAGCCGAGTGTGACAGGTGCGATTGCTCGTTTCTTGACATGTACCACATCATTCAAGATATGGATGCGCAGGTT
TTCTTGTCTCTCATTGATGCAATGGCATCTTCTAACAAAGCGATCATTCACGTTTCTCAGAAAGAGGCGATCAGACAATG
A

Upstream 100 bases:

>100_bases
GCCTTGCTGGATGGCATCTCAGCGGTTACATGCCGTGATGTATTGCAATTCATAGCGACCATCAATTATATCGGAGCTCA
TGTAGTAAGGGGATGAAGAG

Downstream 100 bases:

>100_bases
AACAAAGCACTGTCTTTACCCTTTTATTGCTGCTTATAGGTATGGCGGCTTATTCCTTCGGCTGGGTTCAGGCCGTTGCC
GAAGCCGCCGCCCAATATGT

Product: peptidase involved in subtilosin production

Products: NA

Alternate protein names: Antilisterial bacteriocin subtilosin biosynthesis protein AlbF

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MEKKAFFQQLDERTDIRYTDSGLKIFRLKFPRAHLRLCNVKIDFGSRDVCIRAESGDTLLPYGTAHFLEHLLFWHNGRNL
YSDFFAHGALLNAFTTYTDTNFMFTSLPDRLRQTIPILLDALWNHSFDKKIVAQEKAVITSEIQTAHLNHQLSYHYQLIS
MLSPSSPAAVFPAGRIEDIEALDISDLQKAYKAAYQAHRMTLFLIGGSENTETLLPPHLQLEKRPDYHAERKIIPACPPV
LSQKMMLGDEERMEDTWTGLQIGALPGQNDLLSIKLYWDIAARILFQLDSPFFQEIQQTYRLEIDRLSAETYIYEDGGFL
ILHSQGTHSSAYIDVASYYVTQKKEQVAAWLQYGKDSLTDAIIYDSDYVRKCFEWAAECDRCDCSFLDMYHIIQDMDAQV
FLSLIDAMASSNKAIIHVSQKEAIRQ

Sequences:

>Translated_426_residues
MEKKAFFQQLDERTDIRYTDSGLKIFRLKFPRAHLRLCNVKIDFGSRDVCIRAESGDTLLPYGTAHFLEHLLFWHNGRNL
YSDFFAHGALLNAFTTYTDTNFMFTSLPDRLRQTIPILLDALWNHSFDKKIVAQEKAVITSEIQTAHLNHQLSYHYQLIS
MLSPSSPAAVFPAGRIEDIEALDISDLQKAYKAAYQAHRMTLFLIGGSENTETLLPPHLQLEKRPDYHAERKIIPACPPV
LSQKMMLGDEERMEDTWTGLQIGALPGQNDLLSIKLYWDIAARILFQLDSPFFQEIQQTYRLEIDRLSAETYIYEDGGFL
ILHSQGTHSSAYIDVASYYVTQKKEQVAAWLQYGKDSLTDAIIYDSDYVRKCFEWAAECDRCDCSFLDMYHIIQDMDAQV
FLSLIDAMASSNKAIIHVSQKEAIRQ
>Mature_426_residues
MEKKAFFQQLDERTDIRYTDSGLKIFRLKFPRAHLRLCNVKIDFGSRDVCIRAESGDTLLPYGTAHFLEHLLFWHNGRNL
YSDFFAHGALLNAFTTYTDTNFMFTSLPDRLRQTIPILLDALWNHSFDKKIVAQEKAVITSEIQTAHLNHQLSYHYQLIS
MLSPSSPAAVFPAGRIEDIEALDISDLQKAYKAAYQAHRMTLFLIGGSENTETLLPPHLQLEKRPDYHAERKIIPACPPV
LSQKMMLGDEERMEDTWTGLQIGALPGQNDLLSIKLYWDIAARILFQLDSPFFQEIQQTYRLEIDRLSAETYIYEDGGFL
ILHSQGTHSSAYIDVASYYVTQKKEQVAAWLQYGKDSLTDAIIYDSDYVRKCFEWAAECDRCDCSFLDMYHIIQDMDAQV
FLSLIDAMASSNKAIIHVSQKEAIRQ

Specific function: Required for production of the bacteriocin subtilosin. Could catalyze some step in the processing of presubtilosin

COG id: COG0612

COG function: function code R; Predicted Zn-dependent peptidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M16 family

Homologues:

Organism=Caenorhabditis elegans, GI71999683, Length=149, Percent_Identity=32.2147651006711, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ALBF1_BACSU (P71006)

Other databases:

- EMBL:   Z80360
- EMBL:   AL009126
- PIR:   E70058
- RefSeq:   NP_391622.1
- ProteinModelPortal:   P71006
- EnsemblBacteria:   EBBACT00000000430
- GeneID:   938515
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU37420
- NMPDR:   fig|224308.1.peg.3748
- GenoList:   BSU37420
- ProtClustDB:   CLSK2752550
- BioCyc:   BSUB:BSU37420-MONOMER
- GO:   GO:0006508
- InterPro:   IPR011249
- InterPro:   IPR011237
- InterPro:   IPR011765
- Gene3D:   G3DSA:3.30.830.10

Pfam domain/function: PF00675 Peptidase_M16; SSF63411 Metalloenz_metal-bd

EC number: 3.4.99.- [C]

Molecular weight: Translated: 48982; Mature: 48982

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00143 INSULINASE

Important sites: ACT_SITE 69-69

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKAFFQQLDERTDIRYTDSGLKIFRLKFPRAHLRLCNVKIDFGSRDVCIRAESGDTLL
CCHHHHHHHHHCCCCCEEECCCCEEEEEECCHHHEEEEEEEEECCCCCEEEEECCCCEEC
PYGTAHFLEHLLFWHNGRNLYSDFFAHGALLNAFTTYTDTNFMFTSLPDRLRQTIPILLD
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHH
ALWNHSFDKKIVAQEKAVITSEIQTAHLNHQLSYHYQLISMLSPSSPAAVFPAGRIEDIE
HHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEECCCCCCCCCC
ALDISDLQKAYKAAYQAHRMTLFLIGGSENTETLLPPHLQLEKRPDYHAERKIIPACPPV
CCCHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
LSQKMMLGDEERMEDTWTGLQIGALPGQNDLLSIKLYWDIAARILFQLDSPFFQEIQQTY
HHHHHHCCCHHHHHHHHCCEEEECCCCCCCEEEEEEHHHHHHHHHHHCCCHHHHHHHHHH
RLEIDRLSAETYIYEDGGFLILHSQGTHSSAYIDVASYYVTQKKEQVAAWLQYGKDSLTD
HHHHEEECCEEEEEECCCEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCHHHCC
AIIYDSDYVRKCFEWAAECDRCDCSFLDMYHIIQDMDAQVFLSLIDAMASSNKAIIHVSQ
EEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEH
KEAIRQ
HHHHCC
>Mature Secondary Structure
MEKKAFFQQLDERTDIRYTDSGLKIFRLKFPRAHLRLCNVKIDFGSRDVCIRAESGDTLL
CCHHHHHHHHHCCCCCEEECCCCEEEEEECCHHHEEEEEEEEECCCCCEEEEECCCCEEC
PYGTAHFLEHLLFWHNGRNLYSDFFAHGALLNAFTTYTDTNFMFTSLPDRLRQTIPILLD
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHH
ALWNHSFDKKIVAQEKAVITSEIQTAHLNHQLSYHYQLISMLSPSSPAAVFPAGRIEDIE
HHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEECCCCCCCCCC
ALDISDLQKAYKAAYQAHRMTLFLIGGSENTETLLPPHLQLEKRPDYHAERKIIPACPPV
CCCHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
LSQKMMLGDEERMEDTWTGLQIGALPGQNDLLSIKLYWDIAARILFQLDSPFFQEIQQTY
HHHHHHCCCHHHHHHHHCCEEEECCCCCCCEEEEEEHHHHHHHHHHHCCCHHHHHHHHHH
RLEIDRLSAETYIYEDGGFLILHSQGTHSSAYIDVASYYVTQKKEQVAAWLQYGKDSLTD
HHHHEEECCEEEEEECCCEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCHHHCC
AIIYDSDYVRKCFEWAAECDRCDCSFLDMYHIIQDMDAQVFLSLIDAMASSNKAIIHVSQ
EEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEH
KEAIRQ
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 10572140; 10809709; 10809710