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The map label for this gene is moaA

Identifier: 16080723

GI number: 16080723

Start: 3772325

End: 3773350

Strand: Reverse

Name: moaA

Synonym: BSU36700

Alternate gene names: 16080723

Gene position: 3773350-3772325 (Counterclockwise)

Preceding gene: 16080724

Following gene: 16080722

Centisome position: 89.51

GC content: 46.3

Gene sequence:

>1026_bases
ATGATTACAGAGTCGCGTCAGATGTTGGATAAAAGAAACAGACCGCTGAGAGACCTTCGCATTTCTGTTACAGACCGCTG
CAACTTCCGATGCACGTACTGTATGCCCGCCGAATTATTCGGCCCGGATTATCCTTTTTTAAAGAAAGAGGAGCTGCTTT
CATTTGAGGAGCTGGAACGGCTTGCGACATTGTTTGTCACAAGATTTGGCGTTGAAAAAATCCGCTTGACCGGCGGAGAG
CCCCTCATGAGAAAGGATATGCCGGAATTAATCAAAAAACTGGCCCGCATACCGGGTATTCGTGACATCGCGATGACGAC
AAACGGATCACTGCTGCCTGTTTATGCTAAGAGGCTCAAGGAGGCTGGGCTGAAAAGGGTTACGATCAGCCTTGATTCTT
TAGAAGACGAGCGTTTTAAGAAGATAAACGGGCGCGGTGTTTCTGTCAGCAAGGTGCTGGAAGGCATTGAAGCGGCGAAG
CAAGCGGGGCTCGGTGTTAAAATCAATATGGTCGTTCAAAAGGGCGTCAATGAAAAAGATATACTGCCTATGGCCCGTTA
TTTTAAAGAAAAAGGGCATATCCTGAGATTCATTGAATTTATGGACGTAGGCAACACCAACCAATGGGAAAAGAAAGACG
TCATGACAAAGGCGGAAATCATAGATTTGATCAATAAACACATGCCGGTTGAGCCGATAGCGCCTAATTATATAGGCGAA
GTGGCTTCCAGATTCCGCTACTTGGACGGCTCCGGAGAAATTGGCGTCATATCATCTGTATCTGATGCATTCTGCGGATC
TTGCAACAGAGCCCGTCTTTCTGCGAGAGGAGAGCTGTTTACCTGCTTATTCGCTTCAAGCGGATTTGACCTCAGAGCTC
CGGTTCGCCAGGAATTAAGTGATGACGAACTATCAGAAATGATCGGGACAGTATGGAAAAATCGGATCGATCAGTATTCA
GTCGATCGTACGCTATCAAAAGCATCTGGCAAAAAGAAAGTGGAAATGTCTTATATTGGCGGTTAA

Upstream 100 bases:

>100_bases
TGAAGAATTGAATATCACAGCGATTGGATTTGTCAGAAACGGATCATTTAATGTATATACACACCCTGAGAGAATCAGGG
AGTAGGAAGGGGCTGTAGCT

Downstream 100 bases:

>100_bases
TTTGAAGTCAAAAGCTTATCGGCACTGTCCGGTAAGCTTTTTTATTAGAATCCGAGTGCAAAAAATACGTAGAAAAACCG
AAATATATAAGGATAGGCAT

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: Protein narA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MITESRQMLDKRNRPLRDLRISVTDRCNFRCTYCMPAELFGPDYPFLKKEELLSFEELERLATLFVTRFGVEKIRLTGGE
PLMRKDMPELIKKLARIPGIRDIAMTTNGSLLPVYAKRLKEAGLKRVTISLDSLEDERFKKINGRGVSVSKVLEGIEAAK
QAGLGVKINMVVQKGVNEKDILPMARYFKEKGHILRFIEFMDVGNTNQWEKKDVMTKAEIIDLINKHMPVEPIAPNYIGE
VASRFRYLDGSGEIGVISSVSDAFCGSCNRARLSARGELFTCLFASSGFDLRAPVRQELSDDELSEMIGTVWKNRIDQYS
VDRTLSKASGKKKVEMSYIGG

Sequences:

>Translated_341_residues
MITESRQMLDKRNRPLRDLRISVTDRCNFRCTYCMPAELFGPDYPFLKKEELLSFEELERLATLFVTRFGVEKIRLTGGE
PLMRKDMPELIKKLARIPGIRDIAMTTNGSLLPVYAKRLKEAGLKRVTISLDSLEDERFKKINGRGVSVSKVLEGIEAAK
QAGLGVKINMVVQKGVNEKDILPMARYFKEKGHILRFIEFMDVGNTNQWEKKDVMTKAEIIDLINKHMPVEPIAPNYIGE
VASRFRYLDGSGEIGVISSVSDAFCGSCNRARLSARGELFTCLFASSGFDLRAPVRQELSDDELSEMIGTVWKNRIDQYS
VDRTLSKASGKKKVEMSYIGG
>Mature_341_residues
MITESRQMLDKRNRPLRDLRISVTDRCNFRCTYCMPAELFGPDYPFLKKEELLSFEELERLATLFVTRFGVEKIRLTGGE
PLMRKDMPELIKKLARIPGIRDIAMTTNGSLLPVYAKRLKEAGLKRVTISLDSLEDERFKKINGRGVSVSKVLEGIEAAK
QAGLGVKINMVVQKGVNEKDILPMARYFKEKGHILRFIEFMDVGNTNQWEKKDVMTKAEIIDLINKHMPVEPIAPNYIGE
VASRFRYLDGSGEIGVISSVSDAFCGSCNRARLSARGELFTCLFASSGFDLRAPVRQELSDDELSEMIGTVWKNRIDQYS
VDRTLSKASGKKKVEMSYIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z. Required for both nitrate assimilation and respiration

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI116734668, Length=335, Percent_Identity=37.910447761194, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI22027536, Length=335, Percent_Identity=37.910447761194, Blast_Score=203, Evalue=2e-52,
Organism=Escherichia coli, GI1786999, Length=338, Percent_Identity=32.2485207100592, Blast_Score=174, Evalue=9e-45,
Organism=Caenorhabditis elegans, GI17561210, Length=303, Percent_Identity=39.2739273927393, Blast_Score=207, Evalue=6e-54,
Organism=Caenorhabditis elegans, GI32566331, Length=323, Percent_Identity=37.4613003095975, Blast_Score=203, Evalue=9e-53,
Organism=Caenorhabditis elegans, GI32566329, Length=303, Percent_Identity=37.953795379538, Blast_Score=199, Evalue=1e-51,
Organism=Drosophila melanogaster, GI28574488, Length=347, Percent_Identity=35.4466858789625, Blast_Score=206, Evalue=1e-53,
Organism=Drosophila melanogaster, GI28574490, Length=326, Percent_Identity=35.8895705521472, Blast_Score=206, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_BACSU (P39757)

Other databases:

- EMBL:   Z35277
- EMBL:   Z81356
- EMBL:   AL009126
- PIR:   D69664
- RefSeq:   NP_391551.1
- ProteinModelPortal:   P39757
- SMR:   P39757
- EnsemblBacteria:   EBBACT00000000974
- GeneID:   936982
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU36700
- NMPDR:   fig|224308.1.peg.3677
- GenoList:   BSU36700
- GeneTree:   EBGT00050000002384
- HOGENOM:   HBG529885
- OMA:   VSIGVRK
- ProtClustDB:   PRK00164
- BioCyc:   BSUB:BSU36700-MONOMER
- HAMAP:   MF_01225_B
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02666

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 38550; Mature: 38550

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: BINDING 20-20 BINDING 33-33 BINDING 75-75 BINDING 79-79 BINDING 106-106 BINDING 130-130 BINDING 167-167 BINDING 201-201

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITESRQMLDKRNRPLRDLRISVTDRCNFRCTYCMPAELFGPDYPFLKKEELLSFEELER
CCCCHHHHHHHCCCCHHHEEEEEECCCCCEEEEECCHHHCCCCCCCCCHHHHCCHHHHHH
LATLFVTRFGVEKIRLTGGEPLMRKDMPELIKKLARIPGIRDIAMTTNGSLLPVYAKRLK
HHHHHHHHHCCCEEEECCCCCHHHCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHH
EAGLKRVTISLDSLEDERFKKINGRGVSVSKVLEGIEAAKQAGLGVKINMVVQKGVNEKD
HCCCEEEEEEHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHH
ILPMARYFKEKGHILRFIEFMDVGNTNQWEKKDVMTKAEIIDLINKHMPVEPIAPNYIGE
HHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
VASRFRYLDGSGEIGVISSVSDAFCGSCNRARLSARGELFTCLFASSGFDLRAPVRQELS
HHHHHEECCCCCCEEEECCCHHHHHCCCCCHHHCCCCCEEEEEEECCCCCCCCCHHHCCC
DDELSEMIGTVWKNRIDQYSVDRTLSKASGKKKVEMSYIGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
>Mature Secondary Structure
MITESRQMLDKRNRPLRDLRISVTDRCNFRCTYCMPAELFGPDYPFLKKEELLSFEELER
CCCCHHHHHHHCCCCHHHEEEEEECCCCCEEEEECCHHHCCCCCCCCCHHHHCCHHHHHH
LATLFVTRFGVEKIRLTGGEPLMRKDMPELIKKLARIPGIRDIAMTTNGSLLPVYAKRLK
HHHHHHHHHCCCEEEECCCCCHHHCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHH
EAGLKRVTISLDSLEDERFKKINGRGVSVSKVLEGIEAAKQAGLGVKINMVVQKGVNEKD
HCCCEEEEEEHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHH
ILPMARYFKEKGHILRFIEFMDVGNTNQWEKKDVMTKAEIIDLINKHMPVEPIAPNYIGE
HHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
VASRFRYLDGSGEIGVISSVSDAFCGSCNRARLSARGELFTCLFASSGFDLRAPVRQELS
HHHHHEECCCCCCEEEECCCHHHHHCCCCCHHHCCCCCEEEEEEECCCCCCCCCHHHCCC
DDELSEMIGTVWKNRIDQYSVDRTLSKASGKKKVEMSYIGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7860592; 9384377