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The map label for this gene is pgsA

Identifier: 16080641

GI number: 16080641

Start: 3697821

End: 3698963

Strand: Reverse

Name: pgsA

Synonym: BSU35880

Alternate gene names: 16080641

Gene position: 3698963-3697821 (Counterclockwise)

Preceding gene: 16080642

Following gene: 16080640

Centisome position: 87.74

GC content: 43.74

Gene sequence:

>1143_bases
ATGAAAAAAGAACTGAGCTTTCATGAAAAGCTGCTAAAGCTGACAAAACAGCAAAAAAAGAAAACCAATAAGCACGTATT
TATTGCCATTCCGATCGTTTTTGTCCTTATGTTCGCTTTCATGTGGGCGGGAAAAGCGGAAACGCCGAAGGTCAAAACGT
ATTCTGACGACGTACTCTCAGCCTCATTTGTAGGCGATATTATGATGGGACGCTATGTTGAAAAAGTAACGGAGCAAAAA
GGGGCAGACAGTATTTTTCAATATGTTGAACCGATCTTTAGAGCCTCGGATTATGTAGCAGGAAACTTTGAAAACCCGGT
AACCTATCAAAAGAATTATAAACAAGCAGATAAAGAGATTCATCTGCAGACGAATAAGGAATCAGTGAAAGTCTTGAAGG
ATATGAATTTCACGGTTCTCAACAGCGCCAACAACCACGCAATGGATTACGGCGTTCAGGGCATGAAAGATACGCTTGGA
GAATTTGCGAAGCAAAATCTTGATATCGTTGGAGCGGGATACAGCTTAAGTGATGCGAAAAAGAAAATTTCGTACCAGAA
AGTCAACGGGGTAACGATTGCGACGCTTGGCTTTACCGATGTGTCCGGGAAAGGTTTCGCGGCTAAAAAGAATACGCCGG
GCGTGCTGCCCGCAGATCCTGAAATCTTCATCCCTATGATTTCAGAAGCGAAAAAACATGCGGACATTGTTGTTGTGCAG
TCACACTGGGGACAAGAGTATGACAATGATCCAAATGACCGCCAGCGCCAGCTTGCAAGAGCCATGTCTGATGCGGGAGC
TGACATCATCGTCGGCCATCACCCGCACGTCTTAGAACCGATTGAAGTATATAACGGAACCGTCATTTTCTACAGCCTCG
GCAACTTTGTCTTTGACCAAGGCTGGACGAGAACAAGAGACAGTGCACTGGTTCAGTATCACCTGAAGAAAAATGGAACA
GGACGCTTTGAAGTGACACCGATCGATATCCATGAAGCGACACCTGCGCCTGTGAAAAAAGACAGCCTTAAACAGAAAAC
CATTATTCGCGAACTGACGAAAGACTCTAATTTCGCTTGGAAAGTAGAAGACGGAAAACTGACGTTTGATATTGATCATA
GTGACAAACTAAAATCTAAATAA

Upstream 100 bases:

>100_bases
AGGTTTAACCATTACGTTCGGAAGCACGCTGCTATTGAGCGGAGCGACCTTTGCTATCATGTTTGTTTACTACTTAATTT
AATGTAAGGTGTGTCAAACG

Downstream 100 bases:

>100_bases
TCGGAGTGATAAAGATGAAATTTGTCAAAGCCATCTGGCCGTTTGTTGCCGTAGCCATCGTGTTCATGTTTATGTCAGCT
TTTAAATTCAATGATCAGCT

Product: capsular polyglutamate synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MKKELSFHEKLLKLTKQQKKKTNKHVFIAIPIVFVLMFAFMWAGKAETPKVKTYSDDVLSASFVGDIMMGRYVEKVTEQK
GADSIFQYVEPIFRASDYVAGNFENPVTYQKNYKQADKEIHLQTNKESVKVLKDMNFTVLNSANNHAMDYGVQGMKDTLG
EFAKQNLDIVGAGYSLSDAKKKISYQKVNGVTIATLGFTDVSGKGFAAKKNTPGVLPADPEIFIPMISEAKKHADIVVVQ
SHWGQEYDNDPNDRQRQLARAMSDAGADIIVGHHPHVLEPIEVYNGTVIFYSLGNFVFDQGWTRTRDSALVQYHLKKNGT
GRFEVTPIDIHEATPAPVKKDSLKQKTIIRELTKDSNFAWKVEDGKLTFDIDHSDKLKSK

Sequences:

>Translated_380_residues
MKKELSFHEKLLKLTKQQKKKTNKHVFIAIPIVFVLMFAFMWAGKAETPKVKTYSDDVLSASFVGDIMMGRYVEKVTEQK
GADSIFQYVEPIFRASDYVAGNFENPVTYQKNYKQADKEIHLQTNKESVKVLKDMNFTVLNSANNHAMDYGVQGMKDTLG
EFAKQNLDIVGAGYSLSDAKKKISYQKVNGVTIATLGFTDVSGKGFAAKKNTPGVLPADPEIFIPMISEAKKHADIVVVQ
SHWGQEYDNDPNDRQRQLARAMSDAGADIIVGHHPHVLEPIEVYNGTVIFYSLGNFVFDQGWTRTRDSALVQYHLKKNGT
GRFEVTPIDIHEATPAPVKKDSLKQKTIIRELTKDSNFAWKVEDGKLTFDIDHSDKLKSK
>Mature_380_residues
MKKELSFHEKLLKLTKQQKKKTNKHVFIAIPIVFVLMFAFMWAGKAETPKVKTYSDDVLSASFVGDIMMGRYVEKVTEQK
GADSIFQYVEPIFRASDYVAGNFENPVTYQKNYKQADKEIHLQTNKESVKVLKDMNFTVLNSANNHAMDYGVQGMKDTLG
EFAKQNLDIVGAGYSLSDAKKKISYQKVNGVTIATLGFTDVSGKGFAAKKNTPGVLPADPEIFIPMISEAKKHADIVVVQ
SHWGQEYDNDPNDRQRQLARAMSDAGADIIVGHHPHVLEPIEVYNGTVIFYSLGNFVFDQGWTRTRDSALVQYHLKKNGT
GRFEVTPIDIHEATPAPVKKDSLKQKTIIRELTKDSNFAWKVEDGKLTFDIDHSDKLKSK

Specific function: Seems to be required for maximum PGA (gamma-polyglutamic acid) production

COG id: COG2843

COG function: function code M; Putative enzyme of poly-gamma-glutamate biosynthesis (capsule formation)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CapA family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CAPA_BACSU (P96738)

Other databases:

- EMBL:   Z92954
- EMBL:   AB016245
- EMBL:   AB039950
- EMBL:   AB046355
- EMBL:   AL009126
- PIR:   H70069
- RefSeq:   NP_391469.1
- ProteinModelPortal:   P96738
- EnsemblBacteria:   EBBACT00000000599
- GeneID:   936830
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU35880
- NMPDR:   fig|224308.1.peg.3595
- GenoList:   BSU35880
- GeneTree:   EBGT00050000002588
- HOGENOM:   HBG338376
- OMA:   DSALVQY
- ProtClustDB:   CLSK862265
- BioCyc:   BSUB:BSU35880-MONOMER
- InterPro:   IPR019079
- SMART:   SM00854

Pfam domain/function: PF09587 PGA_cap

EC number: NA

Molecular weight: Translated: 42760; Mature: 42760

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKELSFHEKLLKLTKQQKKKTNKHVFIAIPIVFVLMFAFMWAGKAETPKVKTYSDDVLS
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHCCCCCCCCEEECCCHHHH
ASFVGDIMMGRYVEKVTEQKGADSIFQYVEPIFRASDYVAGNFENPVTYQKNYKQADKEI
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCEE
HLQTNKESVKVLKDMNFTVLNSANNHAMDYGVQGMKDTLGEFAKQNLDIVGAGYSLSDAK
EEECCHHHHHHHHHCCEEEEECCCCCCHHHCCCCHHHHHHHHHHCCCEEEECCCCHHHHH
KKISYQKVNGVTIATLGFTDVSGKGFAAKKNTPGVLPADPEIFIPMISEAKKHADIVVVQ
HHHHHEEECCEEEEEEECEECCCCCEEECCCCCCCCCCCCCEEEEHHHHHCCCCCEEEEE
SHWGQEYDNDPNDRQRQLARAMSDAGADIIVGHHPHVLEPIEVYNGTVIFYSLGNFVFDQ
CCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHCCEEEEEECCCHHHCC
GWTRTRDSALVQYHLKKNGTGRFEVTPIDIHEATPAPVKKDSLKQKTIIRELTKDSNFAW
CCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEE
KVEDGKLTFDIDHSDKLKSK
EEECCEEEEEECCCCCCCCC
>Mature Secondary Structure
MKKELSFHEKLLKLTKQQKKKTNKHVFIAIPIVFVLMFAFMWAGKAETPKVKTYSDDVLS
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHCCCCCCCCEEECCCHHHH
ASFVGDIMMGRYVEKVTEQKGADSIFQYVEPIFRASDYVAGNFENPVTYQKNYKQADKEI
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCEE
HLQTNKESVKVLKDMNFTVLNSANNHAMDYGVQGMKDTLGEFAKQNLDIVGAGYSLSDAK
EEECCHHHHHHHHHCCEEEEECCCCCCHHHCCCCHHHHHHHHHHCCCEEEECCCCHHHHH
KKISYQKVNGVTIATLGFTDVSGKGFAAKKNTPGVLPADPEIFIPMISEAKKHADIVVVQ
HHHHHEEECCEEEEEEECEECCCCCEEECCCCCCCCCCCCCEEEEHHHHHCCCCCEEEEE
SHWGQEYDNDPNDRQRQLARAMSDAGADIIVGHHPHVLEPIEVYNGTVIFYSLGNFVFDQ
CCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHCCEEEEEECCCHHHCC
GWTRTRDSALVQYHLKKNGTGRFEVTPIDIHEATPAPVKKDSLKQKTIIRELTKDSNFAW
CCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEE
KVEDGKLTFDIDHSDKLKSK
EEECCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353933; 10486244; 11751809; 9384377