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The map label for this gene is pgdS

Identifier: 16080639

GI number: 16080639

Start: 3696257

End: 3697498

Strand: Reverse

Name: pgdS

Synonym: BSU35860

Alternate gene names: 16080639

Gene position: 3697498-3696257 (Counterclockwise)

Preceding gene: 16080640

Following gene: 255767775

Centisome position: 87.71

GC content: 46.14

Gene sequence:

>1242_bases
GTGAACACACTGGCAAACTGGAAGAAGTTTTTGCTTGTGGCGGTTATCATTTGTTTTTTGGTTCCAATTATGACAAAAGC
GGAGATTGCGGAAGCTGATACATCATCAGAATTGATTGTCAGCGAAGCAAAAAACCTGCTTGGATATCAGTATAAATATG
GCGGGGAAACGCCGAAAGAGGGTTTCGATCCATCAGGATTGATACAATATGTGTTCAGTAAGGCTGATATTCATCTGCCG
AGATCTGTAAACGACCAGTATAAAATCGGAACAGCTGTAAAACCGGAAAACCTGAAGCCGGGTGATATTTTGTTTTTCAA
GAAAGAGGGAAGCACCGGCACTGTTCCGACACATGACGCCCTTTATATCGGAGACGGCCAAATGGTTCACAGTACACAGT
CAAAAGGGGTTATCATCACCAATTACAAAAAAAGCAGCTATTGGAGCGGAACTTATATCGGGGCGAGACGAATCGCTGCC
GATCCGGCAACGGCTGATGTTCCTGTCGTTCAGGAGGCCGAAAAATATATCGGTGTCCCATATGTGTTTGGCGGAAGCAC
GCCGTCAGAGGGCTTTGATTGCTCGGGGCTTGTGCAATATGTGTTTCAACAGGCACTCGGCATTTATCTACCGCGATCAG
CCGAACAGCAGTGGGCAGTGGGCGAGAAGGTAGCCCCTCAGAACATAAAGCCTGGTGATGTCGTCTATTTCAGCAATACG
TATAAAACGGGAATTTCACATGCAGGCATTTATGCGGGCGCAGGCAGGTTCATTCAGGCAAGCCGGTCAGAAAAAGTAAC
CATTTCCTATTTGTCAGAGGATTACTGGAAATCGAAGATGACGGGTATTCGCCGATTTGACAACCTGACAATCCCGAAAG
AAAATCCGATTGTTTCCGAAGCGACGCTTTATGTCGGAGAAGTGCCTTACAAACAGGGCGGAGTAACACCTGAGACGGGA
TTTGATACAGCTGGATTTGTCCAATATGTATACCAGAAAGCAGCCGGTATTTCCCTGCCTCGATACGCAACAAGCCAGTA
CAATGCCGGAACTAAGATTGAGAAGGCGGACCTGAAGCCGGGAGACATTGTGTTCTTTCAATCAACAAGCTTAAATCCCT
CCATCTATATCGGAAACGGACAAGTTGTTCATGTCACATTATCAAACGGCGTGACCATTACCAATATGAACACGAGCACA
TATTGGAAGGATAAATACGCAGGAAGTATACGGGTGCAATAA

Upstream 100 bases:

>100_bases
TTGCCCAGCATATTCATTCTGTAAAAATATTAAAAAGTCTCTTAACATATTCAAAAAAATACCGATGTTAAAACTGCAAA
AAGAGGAGGAGATAATAAAA

Downstream 100 bases:

>100_bases
AAGAAAAAGACTCCAATCTCTGGAGTCTTTTCTTTATGCATATTGCCCCGAAAGAAGCTGGTCTATAAAGTAGGCCACAC
CATTTTCGTCATTGCTTTTT

Product: gamma-DL-glutamyl hydrolase

Products: NA

Alternate protein names: Poly-gamma-glutamate depolymerase; PGA depolymerase

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MNTLANWKKFLLVAVIICFLVPIMTKAEIAEADTSSELIVSEAKNLLGYQYKYGGETPKEGFDPSGLIQYVFSKADIHLP
RSVNDQYKIGTAVKPENLKPGDILFFKKEGSTGTVPTHDALYIGDGQMVHSTQSKGVIITNYKKSSYWSGTYIGARRIAA
DPATADVPVVQEAEKYIGVPYVFGGSTPSEGFDCSGLVQYVFQQALGIYLPRSAEQQWAVGEKVAPQNIKPGDVVYFSNT
YKTGISHAGIYAGAGRFIQASRSEKVTISYLSEDYWKSKMTGIRRFDNLTIPKENPIVSEATLYVGEVPYKQGGVTPETG
FDTAGFVQYVYQKAAGISLPRYATSQYNAGTKIEKADLKPGDIVFFQSTSLNPSIYIGNGQVVHVTLSNGVTITNMNTST
YWKDKYAGSIRVQ

Sequences:

>Translated_413_residues
MNTLANWKKFLLVAVIICFLVPIMTKAEIAEADTSSELIVSEAKNLLGYQYKYGGETPKEGFDPSGLIQYVFSKADIHLP
RSVNDQYKIGTAVKPENLKPGDILFFKKEGSTGTVPTHDALYIGDGQMVHSTQSKGVIITNYKKSSYWSGTYIGARRIAA
DPATADVPVVQEAEKYIGVPYVFGGSTPSEGFDCSGLVQYVFQQALGIYLPRSAEQQWAVGEKVAPQNIKPGDVVYFSNT
YKTGISHAGIYAGAGRFIQASRSEKVTISYLSEDYWKSKMTGIRRFDNLTIPKENPIVSEATLYVGEVPYKQGGVTPETG
FDTAGFVQYVYQKAAGISLPRYATSQYNAGTKIEKADLKPGDIVFFQSTSLNPSIYIGNGQVVHVTLSNGVTITNMNTST
YWKDKYAGSIRVQ
>Mature_413_residues
MNTLANWKKFLLVAVIICFLVPIMTKAEIAEADTSSELIVSEAKNLLGYQYKYGGETPKEGFDPSGLIQYVFSKADIHLP
RSVNDQYKIGTAVKPENLKPGDILFFKKEGSTGTVPTHDALYIGDGQMVHSTQSKGVIITNYKKSSYWSGTYIGARRIAA
DPATADVPVVQEAEKYIGVPYVFGGSTPSEGFDCSGLVQYVFQQALGIYLPRSAEQQWAVGEKVAPQNIKPGDVVYFSNT
YKTGISHAGIYAGAGRFIQASRSEKVTISYLSEDYWKSKMTGIRRFDNLTIPKENPIVSEATLYVGEVPYKQGGVTPETG
FDTAGFVQYVYQKAAGISLPRYATSQYNAGTKIEKADLKPGDIVFFQSTSLNPSIYIGNGQVVHVTLSNGVTITNMNTST
YWKDKYAGSIRVQ

Specific function: Cleaves, in an endo-type manner, the gamma-glutamyl bond between D-glutamate and L-glutamate of poly-gamma-glutamate (PGA)

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Secreted. Secreted, cell wall. Note=Cell wall localization shown in PubMed:11987133

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family

Homologues:

Organism=Escherichia coli, GI1787944, Length=158, Percent_Identity=36.0759493670886, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786421, Length=120, Percent_Identity=36.6666666666667, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788501, Length=108, Percent_Identity=44.4444444444444, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788001, Length=126, Percent_Identity=32.5396825396825, Blast_Score=76, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PGDS_BACSU (P96740)

Other databases:

- EMBL:   AB085821
- EMBL:   AB080748
- EMBL:   Z92954
- EMBL:   AL009126
- PIR:   B70070
- RefSeq:   NP_391467.1
- ProteinModelPortal:   P96740
- SMR:   P96740
- MEROPS:   C40.005
- EnsemblBacteria:   EBBACT00000001427
- GeneID:   936837
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU35860
- NMPDR:   fig|224308.1.peg.3593
- GenoList:   BSU35860
- GeneTree:   EBGT00070000031872
- HOGENOM:   HBG338374
- OMA:   WKDKYAG
- PhylomeDB:   P96740
- ProtClustDB:   CLSK887986
- BioCyc:   BSUB:BSU35860-MONOMER
- InterPro:   IPR000064

Pfam domain/function: PF00877 NLPC_P60

EC number: NA

Molecular weight: Translated: 45248; Mature: 45248

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLANWKKFLLVAVIICFLVPIMTKAEIAEADTSSELIVSEAKNLLGYQYKYGGETPKE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCEEEECCCCCCCC
GFDPSGLIQYVFSKADIHLPRSVNDQYKIGTAVKPENLKPGDILFFKKEGSTGTVPTHDA
CCCHHHHHHHHHHHCCEECCCCCCCCEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCE
LYIGDGQMVHSTQSKGVIITNYKKSSYWSGTYIGARRIAADPATADVPVVQEAEKYIGVP
EEECCCCEEECCCCCCEEEEECCCCCCCCCEEECCEEEECCCCCCCCCHHHHHHHHCCCC
YVFGGSTPSEGFDCSGLVQYVFQQALGIYLPRSAEQQWAVGEKVAPQNIKPGDVVYFSNT
EEECCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCHHHCCCCCCCCCCCCCCCEEEECCC
YKTGISHAGIYAGAGRFIQASRSEKVTISYLSEDYWKSKMTGIRRFDNLTIPKENPIVSE
HHCCCCCCCEEECCCCEEECCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
ATLYVGEVPYKQGGVTPETGFDTAGFVQYVYQKAAGISLPRYATSQYNAGTKIEKADLKP
EEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCC
GDIVFFQSTSLNPSIYIGNGQVVHVTLSNGVTITNMNTSTYWKDKYAGSIRVQ
CCEEEEECCCCCCEEEECCCEEEEEEECCCEEEEECCCCCEECCCCCCEEEEC
>Mature Secondary Structure
MNTLANWKKFLLVAVIICFLVPIMTKAEIAEADTSSELIVSEAKNLLGYQYKYGGETPKE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCEEEECCCCCCCC
GFDPSGLIQYVFSKADIHLPRSVNDQYKIGTAVKPENLKPGDILFFKKEGSTGTVPTHDA
CCCHHHHHHHHHHHCCEECCCCCCCCEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCE
LYIGDGQMVHSTQSKGVIITNYKKSSYWSGTYIGARRIAADPATADVPVVQEAEKYIGVP
EEECCCCEEECCCCCCEEEEECCCCCCCCCEEECCEEEECCCCCCCCCHHHHHHHHCCCC
YVFGGSTPSEGFDCSGLVQYVFQQALGIYLPRSAEQQWAVGEKVAPQNIKPGDVVYFSNT
EEECCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCHHHCCCCCCCCCCCCCCCEEEECCC
YKTGISHAGIYAGAGRFIQASRSEKVTISYLSEDYWKSKMTGIRRFDNLTIPKENPIVSE
HHCCCCCCCEEECCCCEEECCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
ATLYVGEVPYKQGGVTPETGFDTAGFVQYVYQKAAGISLPRYATSQYNAGTKIEKADLKP
EEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCC
GDIVFFQSTSLNPSIYIGNGQVVHVTLSNGVTITNMNTSTYWKDKYAGSIRVQ
CCEEEEECCCCCCEEEECCCEEEEEEECCCEEEEECCCCCEECCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12073126; 12644511; 9353933; 9384377; 10658653