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The map label for this gene is tagF

Identifier: 16080625

GI number: 16080625

Start: 3676159

End: 3678399

Strand: Reverse

Name: tagF

Synonym: BSU35720

Alternate gene names: 16080625

Gene position: 3678399-3676159 (Counterclockwise)

Preceding gene: 16080626

Following gene: 16080624

Centisome position: 87.26

GC content: 34.23

Gene sequence:

>2241_bases
ATGTCCTTAGTAGTTGACACTAATAAAAGGAAGCAAAAAGGAAAGAGCTTTTATACAGAGGAGCAGAAAAAAGTAATGAT
TGAAAACACTGTGATTAAATGTATTTTGAAAAGCTTGAAAAACAATTTAGGAAGTCTTGAATTGTTAATCTCAATTGATT
CAGAACACCAATTTTTAGAGGATTACCAGTTATTTTTAAAGCTGAAAGAGAGACGTTCAGGAACGGAATCTGAATTTCCG
CTTCAAAACACTGGCTCATTAGAGTATAAAACTGAGATAAATGCTCATGTTTTGCCTATGCCTGTTGAAATGGGACAAAC
ATATGATTTTTATGTCGAATTTCGAAAAAAATATGAAGATGCGGAGCAGGAACCACTCTTGAAGCGTCTTTCTGCTGAAG
TAAATTCAATTGAGCGCGCCTTTCATGTCGATCAAACCACAGAACTTTTGATTTTACCTTATACAACTGATAAAGGCAAC
TTTTCTATTAAGGTGAAAAGAGAGGCCAAAATCATCAGATTTGATCAAATCGAGATTAGCTCTGAAGAAATAAGCATAAC
AGGTTATGCGGGGTACCTGAGTTCCGAAAATCAATATCGGATAAAAAACTTGAACCTTATTTTAAAAAAGGGTGGAGAAA
CACCTATTGAGGAAAAATTTCCAATCAAGCTAGAAAGAAAAACACATGGCCTGGAAAACATGAGAGCAGATGGTTTTGTT
CCGGAACTGTATGATTTTGAAGTGAAAGTGCCTTTGAAAGAAATTCCTTTCTCAAATGAAAAACGTTATGTTTATCGTCT
TTTTATGGAGTATATATGCAATGACGATGAAGGAACGGATATTCAGTTCAACAGCACTGCTCTTGTTTTAGGAGATCGAA
AAAACAAATTAAAAGGATTAGTAAGTATTATTAAAACAAACAACGCACCAGTTCGTTATGAAGTCTTTAAGAAAAAGAAA
AAGCAGACTCTAGGTATCAGAGTAAACGACTATAGCCTGAAAACAAGGATGAAATACTTTATTAAAGGAAAGAAGAAGAG
ATTAGTATCAAAAATAAAAAAGATCACAAAAATGAGAAACAAGTTAATCACTAAAACATACAAATCTCTATTCATGATGG
CTAGCAGAATGCCAGTTAAAAGGAAAACAGTCATTTTTGAAAGTTTTAATGGGAAACAATACAGTTGTAATCCGAGAGCG
ATTTACGAATATATGCGGGAAAACCACCCTGAGTATAAAATGTATTGGAGTGTAAATAAACAATATTCAGCGCCTTTTGA
TGAAAAGGGAATTCCTTACATTAATCGCCTCTCATTAAAATGGCTCTTCGCTATGGCAAGAGCTGAGTATTGGGTTGTTA
ACAGCCGGCTTCCATTATGGATTCCGAAACCTAGTCATACAACATATTTACAAACATGGCATGGCACACCTTTAAAAAGA
CTTGCAATGGATATGGAAGAAGTCCATATGCCTGGTACAAACACCAAAAAATATAAAAGGAATTTTATCAAGGAAGCTTC
TAATTGGGATTACTTGATTTCCCCAAATGGTTATTCAACTGAGATCTTTACACGGGCGTTTCAGTTTAACAAGACAATGA
TTGAATCTGGATATCCTAGAAATGATTTTCTTCATAATGATAATAATGAGGAAACAATATCATTGATAAAGAGTAGGTTA
AATATTCCTCGTGATAAAAAGGTTATTTTATATGCCCCTACATGGAGAGATGATCAGTTCTATGCAAAAGGGCGTTATAA
GTTCGATCTCGATTTAGATTTGCATCAACTTAGACAAGAACTTGGAAATGAATATATTGTAATCTTAAGAATGCATTATC
TGGTAGCTGAGAATTTTGATTTAGGTCCTTTTGAAGGATTTGCATATGATTTTTCTGCTTATGAGGATATTCGAGAATTG
TATATGGTTTCTGATTTGCTGATTACTGATTATTCTTCAGTATTCTTTGATTTTGCAAATTTAAAACGGCCAATGCTATT
CTTTGTCCCTGACATCGAAACCTACCGGGACAAGTTGCGTGGTTTCTACTTTGATTTTGAAAAAGAAGCTCCTGGTCCTT
TGGTAAAAACTACTGAAGAAACGATTGAGGCTATCAAGCAGATCTCATCGCCTGATTATAAGCTTCCGGTTTCTTTTGGT
CCTTTCTATGATAAGTTTTGCTATTTAGAGTCAGGACGTTCATCTGAAAAGGTTGTTAATACTGTATTTAAAGCTGAATA
A

Upstream 100 bases:

>100_bases
CAGGAAACAGTATAGATACTAATAATGGCTATAGAGTAGAGCCCTATACAACAGTTAAAGGCAATTTCTCTTGGAGGGTC
ACGGAAATAAAAGAGAGTTA

Downstream 100 bases:

>100_bases
TTTAGGTTATTTTATAAAATGTATAGCTGTCGATTTTTCGGCAGCTTTTTAGATACTTGTTGAGAAGTGAAAAAAAATGC
AGGATCTGTTACAATAATCA

Product: CDP-glycerol:polyglycerol phosphate glycero-phosphotransferase

Products: NA

Alternate protein names: CGPTase; Major teichoic acid biosynthesis protein F; Polyglycerol phosphate polymerase

Number of amino acids: Translated: 746; Mature: 745

Protein sequence:

>746_residues
MSLVVDTNKRKQKGKSFYTEEQKKVMIENTVIKCILKSLKNNLGSLELLISIDSEHQFLEDYQLFLKLKERRSGTESEFP
LQNTGSLEYKTEINAHVLPMPVEMGQTYDFYVEFRKKYEDAEQEPLLKRLSAEVNSIERAFHVDQTTELLILPYTTDKGN
FSIKVKREAKIIRFDQIEISSEEISITGYAGYLSSENQYRIKNLNLILKKGGETPIEEKFPIKLERKTHGLENMRADGFV
PELYDFEVKVPLKEIPFSNEKRYVYRLFMEYICNDDEGTDIQFNSTALVLGDRKNKLKGLVSIIKTNNAPVRYEVFKKKK
KQTLGIRVNDYSLKTRMKYFIKGKKKRLVSKIKKITKMRNKLITKTYKSLFMMASRMPVKRKTVIFESFNGKQYSCNPRA
IYEYMRENHPEYKMYWSVNKQYSAPFDEKGIPYINRLSLKWLFAMARAEYWVVNSRLPLWIPKPSHTTYLQTWHGTPLKR
LAMDMEEVHMPGTNTKKYKRNFIKEASNWDYLISPNGYSTEIFTRAFQFNKTMIESGYPRNDFLHNDNNEETISLIKSRL
NIPRDKKVILYAPTWRDDQFYAKGRYKFDLDLDLHQLRQELGNEYIVILRMHYLVAENFDLGPFEGFAYDFSAYEDIREL
YMVSDLLITDYSSVFFDFANLKRPMLFFVPDIETYRDKLRGFYFDFEKEAPGPLVKTTEETIEAIKQISSPDYKLPVSFG
PFYDKFCYLESGRSSEKVVNTVFKAE

Sequences:

>Translated_746_residues
MSLVVDTNKRKQKGKSFYTEEQKKVMIENTVIKCILKSLKNNLGSLELLISIDSEHQFLEDYQLFLKLKERRSGTESEFP
LQNTGSLEYKTEINAHVLPMPVEMGQTYDFYVEFRKKYEDAEQEPLLKRLSAEVNSIERAFHVDQTTELLILPYTTDKGN
FSIKVKREAKIIRFDQIEISSEEISITGYAGYLSSENQYRIKNLNLILKKGGETPIEEKFPIKLERKTHGLENMRADGFV
PELYDFEVKVPLKEIPFSNEKRYVYRLFMEYICNDDEGTDIQFNSTALVLGDRKNKLKGLVSIIKTNNAPVRYEVFKKKK
KQTLGIRVNDYSLKTRMKYFIKGKKKRLVSKIKKITKMRNKLITKTYKSLFMMASRMPVKRKTVIFESFNGKQYSCNPRA
IYEYMRENHPEYKMYWSVNKQYSAPFDEKGIPYINRLSLKWLFAMARAEYWVVNSRLPLWIPKPSHTTYLQTWHGTPLKR
LAMDMEEVHMPGTNTKKYKRNFIKEASNWDYLISPNGYSTEIFTRAFQFNKTMIESGYPRNDFLHNDNNEETISLIKSRL
NIPRDKKVILYAPTWRDDQFYAKGRYKFDLDLDLHQLRQELGNEYIVILRMHYLVAENFDLGPFEGFAYDFSAYEDIREL
YMVSDLLITDYSSVFFDFANLKRPMLFFVPDIETYRDKLRGFYFDFEKEAPGPLVKTTEETIEAIKQISSPDYKLPVSFG
PFYDKFCYLESGRSSEKVVNTVFKAE
>Mature_745_residues
SLVVDTNKRKQKGKSFYTEEQKKVMIENTVIKCILKSLKNNLGSLELLISIDSEHQFLEDYQLFLKLKERRSGTESEFPL
QNTGSLEYKTEINAHVLPMPVEMGQTYDFYVEFRKKYEDAEQEPLLKRLSAEVNSIERAFHVDQTTELLILPYTTDKGNF
SIKVKREAKIIRFDQIEISSEEISITGYAGYLSSENQYRIKNLNLILKKGGETPIEEKFPIKLERKTHGLENMRADGFVP
ELYDFEVKVPLKEIPFSNEKRYVYRLFMEYICNDDEGTDIQFNSTALVLGDRKNKLKGLVSIIKTNNAPVRYEVFKKKKK
QTLGIRVNDYSLKTRMKYFIKGKKKRLVSKIKKITKMRNKLITKTYKSLFMMASRMPVKRKTVIFESFNGKQYSCNPRAI
YEYMRENHPEYKMYWSVNKQYSAPFDEKGIPYINRLSLKWLFAMARAEYWVVNSRLPLWIPKPSHTTYLQTWHGTPLKRL
AMDMEEVHMPGTNTKKYKRNFIKEASNWDYLISPNGYSTEIFTRAFQFNKTMIESGYPRNDFLHNDNNEETISLIKSRLN
IPRDKKVILYAPTWRDDQFYAKGRYKFDLDLDLHQLRQELGNEYIVILRMHYLVAENFDLGPFEGFAYDFSAYEDIRELY
MVSDLLITDYSSVFFDFANLKRPMLFFVPDIETYRDKLRGFYFDFEKEAPGPLVKTTEETIEAIKQISSPDYKLPVSFGP
FYDKFCYLESGRSSEKVVNTVFKAE

Specific function: Is responsible for the polymerization of the main chain of the major teichoic acid by sequential transfer of glycerol phosphate units from CDP-glycerol to the dissacharide linkage unit. Synthesizes polymers of approximately 35 glycerol phosphate units in

COG id: COG1887

COG function: function code M; Putative glycosyl/glycerophosphate transferases involved in teichoic acid biosynthesis TagF/TagB/EpsJ/RodC

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CDP-glycerol glycerophosphotransferase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TAGF_BACSU (P13485)

Other databases:

- EMBL:   X15200
- EMBL:   AL009126
- PIR:   S06049
- RefSeq:   NP_391453.1
- ProteinModelPortal:   P13485
- SMR:   P13485
- EnsemblBacteria:   EBBACT00000003004
- GeneID:   936803
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU35720
- NMPDR:   fig|224308.1.peg.3579
- GenoList:   BSU35720
- GeneTree:   EBGT00050000000537
- HOGENOM:   HBG338367
- OMA:   KFCYLES
- ProtClustDB:   CLSK887973
- BioCyc:   BSUB:BSU35720-MONOMER
- BioCyc:   MetaCyc:MONOMER-8821
- BRENDA:   2.7.8.12
- InterPro:   IPR007554

Pfam domain/function: PF04464 Glyphos_transf

EC number: =2.7.8.12

Molecular weight: Translated: 88064; Mature: 87933

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLVVDTNKRKQKGKSFYTEEQKKVMIENTVIKCILKSLKNNLGSLELLISIDSEHQFLE
CEEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHH
DYQLFLKLKERRSGTESEFPLQNTGSLEYKTEINAHVLPMPVEMGQTYDFYVEFRKKYED
HHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCEEEECCHHCCCCCHHHHHHHHHHCC
AEQEPLLKRLSAEVNSIERAFHVDQTTELLILPYTTDKGNFSIKVKREAKIIRFDQIEIS
CCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEEECCCEEEEEEEEEEC
SEEISITGYAGYLSSENQYRIKNLNLILKKGGETPIEEKFPIKLERKTHGLENMRADGFV
CCEEEEEEEEEECCCCCCEEEEEEEEEEECCCCCCCCCCCCEEEEHHHCCHHHCCCCCCC
PELYDFEVKVPLKEIPFSNEKRYVYRLFMEYICNDDEGTDIQFNSTALVLGDRKNKLKGL
CCCCCCEEECCHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCEEEEECCCHHHHHHH
VSIIKTNNAPVRYEVFKKKKKQTLGIRVNDYSLKTRMKYFIKGKKKRLVSKIKKITKMRN
HHHHHCCCCCEEHHHHHHHCCCEECEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
KLITKTYKSLFMMASRMPVKRKTVIFESFNGKQYSCNPRAIYEYMRENHPEYKMYWSVNK
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEECCCHHHHHHHHHCCCCCEEEEEEECC
QYSAPFDEKGIPYINRLSLKWLFAMARAEYWVVNSRLPLWIPKPSHTTYLQTWHGTPLKR
CCCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEEECCCEEEECCCCCCEEEEEECCCHHHH
LAMDMEEVHMPGTNTKKYKRNFIKEASNWDYLISPNGYSTEIFTRAFQFNKTMIESGYPR
HHHCHHHHCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHCHHHHHCCCCC
NDFLHNDNNEETISLIKSRLNIPRDKKVILYAPTWRDDQFYAKGRYKFDLDLDLHQLRQE
CCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCEEEEECCCHHHHHHH
LGNEYIVILRMHYLVAENFDLGPFEGFAYDFSAYEDIRELYMVSDLLITDYSSVFFDFAN
HCCCEEEEEEEEHHHHCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKRPMLFFVPDIETYRDKLRGFYFDFEKEAPGPLVKTTEETIEAIKQISSPDYKLPVSFG
CCCCEEEEECCHHHHHHHHCCEEEEECCCCCCCCEECCHHHHHHHHHHCCCCEEEEEECC
PFYDKFCYLESGRSSEKVVNTVFKAE
CHHHHEEEECCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SLVVDTNKRKQKGKSFYTEEQKKVMIENTVIKCILKSLKNNLGSLELLISIDSEHQFLE
EEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHH
DYQLFLKLKERRSGTESEFPLQNTGSLEYKTEINAHVLPMPVEMGQTYDFYVEFRKKYED
HHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCEEEECCHHCCCCCHHHHHHHHHHCC
AEQEPLLKRLSAEVNSIERAFHVDQTTELLILPYTTDKGNFSIKVKREAKIIRFDQIEIS
CCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEEECCCEEEEEEEEEEC
SEEISITGYAGYLSSENQYRIKNLNLILKKGGETPIEEKFPIKLERKTHGLENMRADGFV
CCEEEEEEEEEECCCCCCEEEEEEEEEEECCCCCCCCCCCCEEEEHHHCCHHHCCCCCCC
PELYDFEVKVPLKEIPFSNEKRYVYRLFMEYICNDDEGTDIQFNSTALVLGDRKNKLKGL
CCCCCCEEECCHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCEEEEECCCHHHHHHH
VSIIKTNNAPVRYEVFKKKKKQTLGIRVNDYSLKTRMKYFIKGKKKRLVSKIKKITKMRN
HHHHHCCCCCEEHHHHHHHCCCEECEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
KLITKTYKSLFMMASRMPVKRKTVIFESFNGKQYSCNPRAIYEYMRENHPEYKMYWSVNK
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEECCCHHHHHHHHHCCCCCEEEEEEECC
QYSAPFDEKGIPYINRLSLKWLFAMARAEYWVVNSRLPLWIPKPSHTTYLQTWHGTPLKR
CCCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEEECCCEEEECCCCCCEEEEEECCCHHHH
LAMDMEEVHMPGTNTKKYKRNFIKEASNWDYLISPNGYSTEIFTRAFQFNKTMIESGYPR
HHHCHHHHCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHCHHHHHCCCCC
NDFLHNDNNEETISLIKSRLNIPRDKKVILYAPTWRDDQFYAKGRYKFDLDLDLHQLRQE
CCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCEEEEECCCHHHHHHH
LGNEYIVILRMHYLVAENFDLGPFEGFAYDFSAYEDIRELYMVSDLLITDYSSVFFDFAN
HCCCEEEEEEEEHHHHCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKRPMLFFVPDIETYRDKLRGFYFDFEKEAPGPLVKTTEETIEAIKQISSPDYKLPVSFG
CCCCEEEEECCHHHHHHHHCCEEEEECCCCCCCCEECCHHHHHHHHHHCCCCEEEEEECC
PFYDKFCYLESGRSSEKVVNTVFKAE
CHHHHEEEECCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2507871; 9384377; 1309530; 12637499