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The map label for this gene is ctpB

Identifier: 16080577

GI number: 16080577

Start: 3622356

End: 3623798

Strand: Reverse

Name: ctpB

Synonym: BSU35240

Alternate gene names: 16080577

Gene position: 3623798-3622356 (Counterclockwise)

Preceding gene: 16080578

Following gene: 16080575

Centisome position: 85.96

GC content: 43.45

Gene sequence:

>1443_bases
ATGAATCAAAAAATAATGGCTGTGATAGCAGCAGGGAGCATGCTGTTCGGAGGCGCCGGTGTATATGCGGGAATCAACCT
GCTTGAGATGGATAAGCCCCAGACTGCAGCAGTTCCCGCGACGGCACAAGCTGACTCTGAACGGGATAAAGCTATGGACA
AAATCGAAAAAGCGTATGAGTTAATTTCGAATGAGTACGTAGAAAAAGTAGATAGAGAAAAGCTGCTCGAGGGAGCCATC
CAGGGAATGCTGTCTACGCTAAATGATCCTTATTCTGTTTACATGGACAAGCAGACAGCTAAGCAATTTTCTGATTCTCT
TGATTCCTCATTTGAAGGCATCGGGGCTGAGGTTGGAATGGAAGACGGCAAAATTATTATTGTTTCTCCATTCAAGAAAT
CGCCAGCGGAAAAAGCCGGGTTGAAGCCTAATGATGAAATTATCAGCATCAATGGAGAATCGATGGCCGGCAAGGATTTA
AACCACGCTGTGTTAAAAATAAGAGGAAAAAAAGGGTCCAGCGTTTCAATGAAGATTCAGCGGCCTGGAACGAAGAAGCA
GTTGTCATTCCGGATCAAGAGAGCTGAGATTCCGCTCGAAACGGTTTTTGCATCAGAGAAAAAAGTACAGGGGCATTCTG
TCGGATATATTGCCATCTCTACTTTTTCTGAGCACACCGCAGAGGATTTTGCAAAAGCGCTGAGGGAGCTTGAGAAAAAA
GAAATAGAAGGACTTGTCATTGATGTACGCGGCAACCCGGGTGGATATCTTCAAAGTGTGGAAGAAATTCTTAAGCATTT
CGTTACAAAGGATCAGCCGTATATTCAGATTGCTGAACGCAATGGAGATAAAAAACGTTATTTTTCAACATTGACCCATA
AAAAAGCGTACCCTGTCAATGTGATTACGGATAAAGGAAGTGCCTCTGCATCAGAAATTCTTGCCGGCGCATTAAAGGAA
GCTGGCCATTATGATGTTGTAGGCGATACGTCTTTTGGAAAGGGAACGGTTCAACAGGCTGTGCCAATGGGGGACGGCAG
CAACATCAAATTAACGCTTTACAAGTGGCTGACGCCAAATGGAAACTGGATTCATAAGAAAGGAATTGAACCGACCATTG
CGATTAAGCAGCCCGATTATTTTTCCGCCGGGCCCTTACAGCTGAAAGAGCCGCTTAAAGTGGACATGAACAATGAAGAC
GTCAAGCATGCGCAGGTATTGTTAAAAGGTCTCAGCTTTGATCCGGGACGTGAGGATGGCTATTTCAGCAAAGACATGAA
AAAAGCGGTTATGGCTTTTCAGGATCAAAACAAGCTGAATAAAACCGGTGTGATCGACACTCGCACTGCAGAGACATTAA
ATCAGCAGATAGAAAAGAAAAAATCAGATGAAAAAAATGATTTGCAGCTTCAAACAGCATTGAAATCATTATTTGTCAAT
TAA

Upstream 100 bases:

>100_bases
TGCCTTCTTTTTCTTCTATGATTACATGAAATCTCCATCCTTTACATATACTATCTCTAGGTTTTGGTAAAGACAGTCTG
GCATACAGGAGGAGTAAAGG

Downstream 100 bases:

>100_bases
AGGAGATGGTATTTCATCTCCTGTTTCTTTGTGCTTAAAGAGATTATGGATCATAAGTCACATAAAAAATGAGTTTTTGA
CAAGAAATTAAAAAAATTTT

Product: PDZ-containing carboxyl-terminal protease processing protease

Products: NA

Alternate protein names: C-terminal processing protease

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MNQKIMAVIAAGSMLFGGAGVYAGINLLEMDKPQTAAVPATAQADSERDKAMDKIEKAYELISNEYVEKVDREKLLEGAI
QGMLSTLNDPYSVYMDKQTAKQFSDSLDSSFEGIGAEVGMEDGKIIIVSPFKKSPAEKAGLKPNDEIISINGESMAGKDL
NHAVLKIRGKKGSSVSMKIQRPGTKKQLSFRIKRAEIPLETVFASEKKVQGHSVGYIAISTFSEHTAEDFAKALRELEKK
EIEGLVIDVRGNPGGYLQSVEEILKHFVTKDQPYIQIAERNGDKKRYFSTLTHKKAYPVNVITDKGSASASEILAGALKE
AGHYDVVGDTSFGKGTVQQAVPMGDGSNIKLTLYKWLTPNGNWIHKKGIEPTIAIKQPDYFSAGPLQLKEPLKVDMNNED
VKHAQVLLKGLSFDPGREDGYFSKDMKKAVMAFQDQNKLNKTGVIDTRTAETLNQQIEKKKSDEKNDLQLQTALKSLFVN

Sequences:

>Translated_480_residues
MNQKIMAVIAAGSMLFGGAGVYAGINLLEMDKPQTAAVPATAQADSERDKAMDKIEKAYELISNEYVEKVDREKLLEGAI
QGMLSTLNDPYSVYMDKQTAKQFSDSLDSSFEGIGAEVGMEDGKIIIVSPFKKSPAEKAGLKPNDEIISINGESMAGKDL
NHAVLKIRGKKGSSVSMKIQRPGTKKQLSFRIKRAEIPLETVFASEKKVQGHSVGYIAISTFSEHTAEDFAKALRELEKK
EIEGLVIDVRGNPGGYLQSVEEILKHFVTKDQPYIQIAERNGDKKRYFSTLTHKKAYPVNVITDKGSASASEILAGALKE
AGHYDVVGDTSFGKGTVQQAVPMGDGSNIKLTLYKWLTPNGNWIHKKGIEPTIAIKQPDYFSAGPLQLKEPLKVDMNNED
VKHAQVLLKGLSFDPGREDGYFSKDMKKAVMAFQDQNKLNKTGVIDTRTAETLNQQIEKKKSDEKNDLQLQTALKSLFVN
>Mature_480_residues
MNQKIMAVIAAGSMLFGGAGVYAGINLLEMDKPQTAAVPATAQADSERDKAMDKIEKAYELISNEYVEKVDREKLLEGAI
QGMLSTLNDPYSVYMDKQTAKQFSDSLDSSFEGIGAEVGMEDGKIIIVSPFKKSPAEKAGLKPNDEIISINGESMAGKDL
NHAVLKIRGKKGSSVSMKIQRPGTKKQLSFRIKRAEIPLETVFASEKKVQGHSVGYIAISTFSEHTAEDFAKALRELEKK
EIEGLVIDVRGNPGGYLQSVEEILKHFVTKDQPYIQIAERNGDKKRYFSTLTHKKAYPVNVITDKGSASASEILAGALKE
AGHYDVVGDTSFGKGTVQQAVPMGDGSNIKLTLYKWLTPNGNWIHKKGIEPTIAIKQPDYFSAGPLQLKEPLKVDMNNED
VKHAQVLLKGLSFDPGREDGYFSKDMKKAVMAFQDQNKLNKTGVIDTRTAETLNQQIEKKKSDEKNDLQLQTALKSLFVN

Specific function: Involved in the signal transduction pathway leading to the proteolytic activation of the mother cell transcription factor pro-sigma-K during sporulation. The signaling serine protease CtpB triggers pro-sigma-K processing by cleaving the regulatory protein

COG id: COG0793

COG function: function code M; Periplasmic protease

Gene ontology:

Cell location: Forespore intermembrane space. Note=Is expressed in both the mother cell and forespore compartments but that synthesis in the forespore is both necessary and sufficient for the proper timing of pro-sigma-K processing

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain

Homologues:

Organism=Escherichia coli, GI1788134, Length=311, Percent_Identity=32.475884244373, Blast_Score=122, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CTPB_BACSU (O35002)

Other databases:

- EMBL:   AF017113
- EMBL:   AL009126
- PIR:   E70042
- RefSeq:   NP_391404.1
- HSSP:   O14745
- ProteinModelPortal:   O35002
- MEROPS:   S41.007
- EnsemblBacteria:   EBBACT00000004007
- GeneID:   936678
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU35240
- NMPDR:   fig|224308.1.peg.3530
- GenoList:   BSU35240
- GeneTree:   EBGT00050000001244
- HOGENOM:   HBG750030
- OMA:   RSIQARG
- ProtClustDB:   CLSK2391285
- BioCyc:   BSUB:BSU35240-MONOMER
- GO:   GO:0006508
- InterPro:   IPR001478
- InterPro:   IPR005151
- InterPro:   IPR004447
- InterPro:   IPR002477
- Gene3D:   G3DSA:1.10.101.10
- SMART:   SM00228
- SMART:   SM00245
- TIGRFAMs:   TIGR00225

Pfam domain/function: PF00595 PDZ; PF03572 Peptidase_S41; PF01471 PG_binding_1; SSF50156 PDZ; SSF47090 PGBD_like

EC number: =3.4.21.102

Molecular weight: Translated: 52799; Mature: 52799

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS50106 PDZ

Important sites: ACT_SITE 309-309 ACT_SITE 320-320 ACT_SITE 334-334

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQKIMAVIAAGSMLFGGAGVYAGINLLEMDKPQTAAVPATAQADSERDKAMDKIEKAYE
CCCEEEEEEEECCHHCCCCHHHHCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LISNEYVEKVDREKLLEGAIQGMLSTLNDPYSVYMDKQTAKQFSDSLDSSFEGIGAEVGM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCC
EDGKIIIVSPFKKSPAEKAGLKPNDEIISINGESMAGKDLNHAVLKIRGKKGSSVSMKIQ
CCCCEEEEECCCCCCHHHCCCCCCCCEEEECCCCCCCCCCCEEEEEEECCCCCEEEEEEE
RPGTKKQLSFRIKRAEIPLETVFASEKKVQGHSVGYIAISTFSEHTAEDFAKALRELEKK
CCCCCCHHEEEEEHHCCCHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHH
EIEGLVIDVRGNPGGYLQSVEEILKHFVTKDQPYIQIAERNGDKKRYFSTLTHKKAYPVN
CCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEE
VITDKGSASASEILAGALKEAGHYDVVGDTSFGKGTVQQAVPMGDGSNIKLTLYKWLTPN
EEECCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHCCCCCCCEEEEEEEEEECCC
GNWIHKKGIEPTIAIKQPDYFSAGPLQLKEPLKVDMNNEDVKHAQVLLKGLSFDPGREDG
CCEEEECCCCCEEEEECCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHCCCCCCCCCC
YFSKDMKKAVMAFQDQNKLNKTGVIDTRTAETLNQQIEKKKSDEKNDLQLQTALKSLFVN
CCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNQKIMAVIAAGSMLFGGAGVYAGINLLEMDKPQTAAVPATAQADSERDKAMDKIEKAYE
CCCEEEEEEEECCHHCCCCHHHHCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LISNEYVEKVDREKLLEGAIQGMLSTLNDPYSVYMDKQTAKQFSDSLDSSFEGIGAEVGM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCC
EDGKIIIVSPFKKSPAEKAGLKPNDEIISINGESMAGKDLNHAVLKIRGKKGSSVSMKIQ
CCCCEEEEECCCCCCHHHCCCCCCCCEEEECCCCCCCCCCCEEEEEEECCCCCEEEEEEE
RPGTKKQLSFRIKRAEIPLETVFASEKKVQGHSVGYIAISTFSEHTAEDFAKALRELEKK
CCCCCCHHEEEEEHHCCCHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHH
EIEGLVIDVRGNPGGYLQSVEEILKHFVTKDQPYIQIAERNGDKKRYFSTLTHKKAYPVN
CCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEE
VITDKGSASASEILAGALKEAGHYDVVGDTSFGKGTVQQAVPMGDGSNIKLTLYKWLTPN
EEECCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHCCCCCCCEEEEEEEEEECCC
GNWIHKKGIEPTIAIKQPDYFSAGPLQLKEPLKVDMNNEDVKHAQVLLKGLSFDPGREDG
CCEEEECCCCCEEEEECCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHCCCCCCCCCC
YFSKDMKKAVMAFQDQNKLNKTGVIDTRTAETLNQQIEKKKSDEKNDLQLQTALKSLFVN
CCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9570401; 9384377