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The map label for this gene is hisF

Identifier: 16080540

GI number: 16080540

Start: 3583562

End: 3584320

Strand: Reverse

Name: hisF

Synonym: BSU34870

Alternate gene names: 16080540

Gene position: 3584320-3583562 (Counterclockwise)

Preceding gene: 16080541

Following gene: 16080539

Centisome position: 85.03

GC content: 50.2

Gene sequence:

>759_bases
ATGATCACAAAACGGATTATCCCATGCCTTGATGTCAAAGAAGGACGCGTTGTCAAAGGAATTCAATTTCTCGAATTGAA
GGATGCCGGTGACCCAGTTGAACTCGCCGAAGTGTACGACCGGGAAGGTGCGGATGAACTTGTTTTTCTTGATATCTCCG
CCTCGCACGAAGGACGAAAGACGATGGTTGACGTTGTGGAGCAGGTTGCCGCGAAGCTTGCCATTCCGTTTACAGTCGGC
GGCGGCATCAATCAGCTCAGCGATATGAAAACGATACTGCGTGCCGGAGCGGACAAAGTGTCGGTGAACACGGCAGCTGT
TCTCCGCCCTGAGCTGATTACAGAAGGAGCCGAGTTTTTCGGTTCTCAATGCATCGTACTTGCCATTGATGCCAAGTATG
ACAAAGAATCTGACACATATAAGGTCTACACGCACGGCGGCAGAAAGAAAACAGATTGGGAGGTCACCGCGTGGGCAAAA
GAAGGCGTCAAACGCGGGGCAGGAGAAATATTGCTGACAAGCATGGACTCCGACGGTGAGAAAAAAGGTTTCGACCACAC
GCTGACAAAGCTTGTTTCCGAAGCTGTCCCCGTCCCTGTTATCGCTTCGGGAGGCGCGGGAAACGCACAGCATATGCTTG
AGGCTTTTACAAAAGGAGAAGCCGACGCCGCGCTGGCCGCCTCAATTTTTCATTATAAAGAAACATCGATTAAAGAAGTG
AAATCATACTTGAAAGAGTACGGGGTGAATGTGAGATGA

Upstream 100 bases:

>100_bases
CCCGCAATGAGGCGGACGGCGTCTCTGGAGCGATCATCGGAAAAGCGCTGTATACCAATCAGTTTACGCTGAGTGAGGCG
CTTGAAAGGGTGAAGCGCAA

Downstream 100 bases:

>100_bases
AACAGGCAGATGAACTGCGTTTTAACGAGGACGGATTAATTCCTGCTATCGTGCAGGATGCGGCAAGCAAAGAAGTGCTG
ACACTTGCGTATATGAACAA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MITKRIIPCLDVKEGRVVKGIQFLELKDAGDPVELAEVYDREGADELVFLDISASHEGRKTMVDVVEQVAAKLAIPFTVG
GGINQLSDMKTILRAGADKVSVNTAAVLRPELITEGAEFFGSQCIVLAIDAKYDKESDTYKVYTHGGRKKTDWEVTAWAK
EGVKRGAGEILLTSMDSDGEKKGFDHTLTKLVSEAVPVPVIASGGAGNAQHMLEAFTKGEADAALAASIFHYKETSIKEV
KSYLKEYGVNVR

Sequences:

>Translated_252_residues
MITKRIIPCLDVKEGRVVKGIQFLELKDAGDPVELAEVYDREGADELVFLDISASHEGRKTMVDVVEQVAAKLAIPFTVG
GGINQLSDMKTILRAGADKVSVNTAAVLRPELITEGAEFFGSQCIVLAIDAKYDKESDTYKVYTHGGRKKTDWEVTAWAK
EGVKRGAGEILLTSMDSDGEKKGFDHTLTKLVSEAVPVPVIASGGAGNAQHMLEAFTKGEADAALAASIFHYKETSIKEV
KSYLKEYGVNVR
>Mature_252_residues
MITKRIIPCLDVKEGRVVKGIQFLELKDAGDPVELAEVYDREGADELVFLDISASHEGRKTMVDVVEQVAAKLAIPFTVG
GGINQLSDMKTILRAGADKVSVNTAAVLRPELITEGAEFFGSQCIVLAIDAKYDKESDTYKVYTHGGRKKTDWEVTAWAK
EGVKRGAGEILLTSMDSDGEKKGFDHTLTKLVSEAVPVPVIASGGAGNAQHMLEAFTKGEADAALAASIFHYKETSIKEV
KSYLKEYGVNVR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=50, Blast_Score=240, Evalue=5e-65,
Organism=Escherichia coli, GI87082028, Length=207, Percent_Identity=27.536231884058, Blast_Score=78, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6319725, Length=316, Percent_Identity=35.126582278481, Blast_Score=157, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_BACSU (O34727)

Other databases:

- EMBL:   AF017113
- EMBL:   AL009126
- PIR:   B69641
- RefSeq:   NP_391367.1
- ProteinModelPortal:   O34727
- SMR:   O34727
- EnsemblBacteria:   EBBACT00000000538
- GeneID:   936554
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU34870
- NMPDR:   fig|224308.1.peg.3493
- GenoList:   BSU34870
- GeneTree:   EBGT00050000001074
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- PhylomeDB:   O34727
- ProtClustDB:   PRK02083
- BioCyc:   BSUB:BSU34870-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27294; Mature: 27294

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITKRIIPCLDVKEGRVVKGIQFLELKDAGDPVELAEVYDREGADELVFLDISASHEGRK
CCCCCCCCCCCCCCCCEECCEEEEEECCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCHH
TMVDVVEQVAAKLAIPFTVGGGINQLSDMKTILRAGADKVSVNTAAVLRPELITEGAEFF
HHHHHHHHHHHHEECCEEECCCHHHHHHHHHHHHCCCCEEECCHHHHHCHHHHHHHHHHH
GSQCIVLAIDAKYDKESDTYKVYTHGGRKKTDWEVTAWAKEGVKRGAGEILLTSMDSDGE
CCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCEEHHHHHHHHHCCCCCEEEEECCCCCC
KKGFDHTLTKLVSEAVPVPVIASGGAGNAQHMLEAFTKGEADAALAASIFHYKETSIKEV
CCCHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
KSYLKEYGVNVR
HHHHHHCCCCCC
>Mature Secondary Structure
MITKRIIPCLDVKEGRVVKGIQFLELKDAGDPVELAEVYDREGADELVFLDISASHEGRK
CCCCCCCCCCCCCCCCEECCEEEEEECCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCHH
TMVDVVEQVAAKLAIPFTVGGGINQLSDMKTILRAGADKVSVNTAAVLRPELITEGAEFF
HHHHHHHHHHHHEECCEEECCCHHHHHHHHHHHHCCCCEEECCHHHHHCHHHHHHHHHHH
GSQCIVLAIDAKYDKESDTYKVYTHGGRKKTDWEVTAWAKEGVKRGAGEILLTSMDSDGE
CCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCEEHHHHHHHHHCCCCCEEEEECCCCCC
KKGFDHTLTKLVSEAVPVPVIASGGAGNAQHMLEAFTKGEADAALAASIFHYKETSIKEV
CCCHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
KSYLKEYGVNVR
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377