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The map label for this gene is malL

Identifier: 16080509

GI number: 16080509

Start: 3547550

End: 3549235

Strand: Reverse

Name: malL

Synonym: BSU34560

Alternate gene names: 16080509

Gene position: 3549235-3547550 (Counterclockwise)

Preceding gene: 16080510

Following gene: 16080508

Centisome position: 84.19

GC content: 43.89

Gene sequence:

>1686_bases
ATGAGTGAATGGTGGAAAGAAGCTGTCGTTTATCAAATTTACCCGCGCAGTTTTTATGATGCCAATGGAGATGGATTCGG
AGATTTGCAAGGTGTGATTCAAAAGCTGGATTACATCAAAAACCTCGGGGCGGATGTCATCTGGCTCTCCCCGGTATTTG
ATTCCCCGCAGGATGACAACGGATATGATATCAGCGATTACAAAAACATGTACGAAAAGTTTGGGACAAATGAAGATATG
TTTCAGCTGATTGATGAAGTCCATAAACGCGGAATGAAAATCGTCATGGATTTGGTCGTGAACCACACATCAGATGAGCA
TGCTTGGTTTGCTGAAAGCCGCAAGTCGAAGGACAATCCTTACCGGGATTATTATCTTTGGAAAGATCCTAAACCGGACG
GCTCAGAGCCGAATAATTGGGGATCGATCTTTTCAGGCTCAGCGTGGACATACGATGAAGGAACAGGGCAGTATTATTTG
CACTACTTTTCGAAAAAACAGCCTGATTTAAATTGGGAAAACGAAGCCGTTCGCCGGGAAGTTTATGATGTAATGAGATT
CTGGATGGATAGAGGCGTTGACGGCTGGCGGATGGATGTGATTGGCTCTATTTCTAAATACACCGATTTTCCGGACTACG
AAACGGATCACAGCCGCAGCTATATTGTCGGCCGTTATCACTCTAACGGCCCACGTCTTCATGAGTTTATTCAAGAGATG
AACAGGGAAGTGCTTTCTCATTACGACTGCATGACAGTCGGAGAGGCAAACGGCTCTGATATTGAAGAGGCGAAAAAGTA
CACAGATGCAAGCAGGCAAGAGCTGAATATGATCTTTACATTTGAACATATGGATATTGATAAAGAACAAAACTCGCCAA
ATGGGAAATGGCAGATCAAGCCGTTTGATTTGATTGCTTTAAAAAAGACGATGACAAGGTGGCAGACCGGGTTAATGAAT
GTCGGCTGGAATACCCTTTATTTTGAGAACCATGACCAGCCGAGGGTTATTTCCCGCTGGGGCAATGACAGGAAACTGCG
TAAGGAATGTGCGAAGGCATTTGCAACGGTTCTGCACGGCATGAAAGGAACGCCGTTTATTTACCAGGGAGAAGAAATCG
GCATGGTCAACAGCGATATGCCGCTGGAGATGTATGATGATCTTGAAATAAAGAATGCATATCGTGAACTAGTCGTCGAA
AACAAAACGATGTCAGAAAAAGAGTTTGTCAAAGCCGTGATGATAAAAGGAAGGGATCATGCGAGAACACCGATGCAGTG
GGATGCCGGAAAACATGCGGGATTCACAGCCGGAGACCCGTGGATACCGGTAAATTCCCGCTATCAAGATATCAATGTGA
AAGAGTCTTTGGAAGATCAAGATTCGATTTTCTTTTACTATCAGAAGCTCATTCAATTACGAAAGCAATATAAGATTATG
ATATATGGCGATTATCAGCTGTTGCAGGAGAATGATCCGCAGGTCTTTTCTTACCTTCGAGAATATCGAGGGGAAAAGCT
TCTTGTCGTTGTGAATTTATCGGAAGAAAAGGCTCTGTTCGAAGCGCCTCCAGAACTGATTCATGAGCGTTGGAAAGTGC
TAATTTCAAACTATCCGCAGGAGCGGGCTGACTTAAAGAGTATTAGCCTCAAACCTTATGAAGCTGTGATGGGCATTAGT
ATATGA

Upstream 100 bases:

>100_bases
AAGCGCGTTATTTTTGAGCTTGTAAAAGGCGAGCCGCTGCACATGAACGTTTATGAGGAACCGGTTGTCCTACAGGGACG
ATGTGAAAGGAGAACGCCTA

Downstream 100 bases:

>100_bases
AAGCGGTCATTTTTGATTTAGACGGAGTGATAACAGATACTGCTGAATATCATTTTCTCGCTTGGAAGCACATCGCAGAA
CAAATCGATATCCCTTTTGA

Product: oligo-1,4-1,6-alpha-glucosidase

Products: NA

Alternate protein names: Dextrin 6-alpha-D-glucanohydrolase; Oligosaccharide alpha-1,6-glucosidase 1; Sucrase-isomaltase 1; Isomaltase 1

Number of amino acids: Translated: 561; Mature: 560

Protein sequence:

>561_residues
MSEWWKEAVVYQIYPRSFYDANGDGFGDLQGVIQKLDYIKNLGADVIWLSPVFDSPQDDNGYDISDYKNMYEKFGTNEDM
FQLIDEVHKRGMKIVMDLVVNHTSDEHAWFAESRKSKDNPYRDYYLWKDPKPDGSEPNNWGSIFSGSAWTYDEGTGQYYL
HYFSKKQPDLNWENEAVRREVYDVMRFWMDRGVDGWRMDVIGSISKYTDFPDYETDHSRSYIVGRYHSNGPRLHEFIQEM
NREVLSHYDCMTVGEANGSDIEEAKKYTDASRQELNMIFTFEHMDIDKEQNSPNGKWQIKPFDLIALKKTMTRWQTGLMN
VGWNTLYFENHDQPRVISRWGNDRKLRKECAKAFATVLHGMKGTPFIYQGEEIGMVNSDMPLEMYDDLEIKNAYRELVVE
NKTMSEKEFVKAVMIKGRDHARTPMQWDAGKHAGFTAGDPWIPVNSRYQDINVKESLEDQDSIFFYYQKLIQLRKQYKIM
IYGDYQLLQENDPQVFSYLREYRGEKLLVVVNLSEEKALFEAPPELIHERWKVLISNYPQERADLKSISLKPYEAVMGIS
I

Sequences:

>Translated_561_residues
MSEWWKEAVVYQIYPRSFYDANGDGFGDLQGVIQKLDYIKNLGADVIWLSPVFDSPQDDNGYDISDYKNMYEKFGTNEDM
FQLIDEVHKRGMKIVMDLVVNHTSDEHAWFAESRKSKDNPYRDYYLWKDPKPDGSEPNNWGSIFSGSAWTYDEGTGQYYL
HYFSKKQPDLNWENEAVRREVYDVMRFWMDRGVDGWRMDVIGSISKYTDFPDYETDHSRSYIVGRYHSNGPRLHEFIQEM
NREVLSHYDCMTVGEANGSDIEEAKKYTDASRQELNMIFTFEHMDIDKEQNSPNGKWQIKPFDLIALKKTMTRWQTGLMN
VGWNTLYFENHDQPRVISRWGNDRKLRKECAKAFATVLHGMKGTPFIYQGEEIGMVNSDMPLEMYDDLEIKNAYRELVVE
NKTMSEKEFVKAVMIKGRDHARTPMQWDAGKHAGFTAGDPWIPVNSRYQDINVKESLEDQDSIFFYYQKLIQLRKQYKIM
IYGDYQLLQENDPQVFSYLREYRGEKLLVVVNLSEEKALFEAPPELIHERWKVLISNYPQERADLKSISLKPYEAVMGIS
I
>Mature_560_residues
SEWWKEAVVYQIYPRSFYDANGDGFGDLQGVIQKLDYIKNLGADVIWLSPVFDSPQDDNGYDISDYKNMYEKFGTNEDMF
QLIDEVHKRGMKIVMDLVVNHTSDEHAWFAESRKSKDNPYRDYYLWKDPKPDGSEPNNWGSIFSGSAWTYDEGTGQYYLH
YFSKKQPDLNWENEAVRREVYDVMRFWMDRGVDGWRMDVIGSISKYTDFPDYETDHSRSYIVGRYHSNGPRLHEFIQEMN
REVLSHYDCMTVGEANGSDIEEAKKYTDASRQELNMIFTFEHMDIDKEQNSPNGKWQIKPFDLIALKKTMTRWQTGLMNV
GWNTLYFENHDQPRVISRWGNDRKLRKECAKAFATVLHGMKGTPFIYQGEEIGMVNSDMPLEMYDDLEIKNAYRELVVEN
KTMSEKEFVKAVMIKGRDHARTPMQWDAGKHAGFTAGDPWIPVNSRYQDINVKESLEDQDSIFFYYQKLIQLRKQYKIMI
YGDYQLLQENDPQVFSYLREYRGEKLLVVVNLSEEKALFEAPPELIHERWKVLISNYPQERADLKSISLKPYEAVMGISI

Specific function: Hydrolyzes various disaccharides such as sucrose, maltose, and isomaltose with different efficiencies. Also hydrolyzes longer maltodextrins from maltotriose up to maltohexaose, but not maltoheptaose, palatinose, isomaltotriose, or isomaltotetraose

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI187423904, Length=541, Percent_Identity=29.5748613678373, Blast_Score=241, Evalue=2e-63,
Organism=Escherichia coli, GI1790687, Length=558, Percent_Identity=45.6989247311828, Blast_Score=517, Evalue=1e-148,
Organism=Escherichia coli, GI1786604, Length=396, Percent_Identity=26.2626262626263, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789995, Length=92, Percent_Identity=33.695652173913, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI32565753, Length=546, Percent_Identity=23.6263736263736, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI25147709, Length=507, Percent_Identity=24.4575936883629, Blast_Score=119, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6321726, Length=584, Percent_Identity=42.6369863013699, Blast_Score=459, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6322241, Length=584, Percent_Identity=41.4383561643836, Blast_Score=443, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6322021, Length=584, Percent_Identity=41.4383561643836, Blast_Score=443, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6324416, Length=584, Percent_Identity=41.6095890410959, Blast_Score=443, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6322245, Length=577, Percent_Identity=41.2478336221837, Blast_Score=442, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6321731, Length=578, Percent_Identity=42.9065743944637, Blast_Score=438, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6319776, Length=578, Percent_Identity=42.9065743944637, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24583745, Length=584, Percent_Identity=32.5342465753425, Blast_Score=278, Evalue=6e-75,
Organism=Drosophila melanogaster, GI24583747, Length=543, Percent_Identity=33.3333333333333, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24583749, Length=543, Percent_Identity=33.3333333333333, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI221330053, Length=597, Percent_Identity=31.9932998324958, Blast_Score=268, Evalue=8e-72,
Organism=Drosophila melanogaster, GI24586599, Length=594, Percent_Identity=33.3333333333333, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24586593, Length=583, Percent_Identity=31.2178387650086, Blast_Score=261, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24586587, Length=588, Percent_Identity=31.4625850340136, Blast_Score=258, Evalue=7e-69,
Organism=Drosophila melanogaster, GI24586591, Length=502, Percent_Identity=34.0637450199203, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24586597, Length=537, Percent_Identity=31.6573556797021, Blast_Score=252, Evalue=5e-67,
Organism=Drosophila melanogaster, GI45549022, Length=537, Percent_Identity=31.6573556797021, Blast_Score=247, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24586589, Length=540, Percent_Identity=31.8518518518519, Blast_Score=239, Evalue=3e-63,
Organism=Drosophila melanogaster, GI281360393, Length=527, Percent_Identity=28.4629981024668, Blast_Score=187, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): O16G1_BACSU (O06994)

Other databases:

- EMBL:   Z94043
- EMBL:   AL009126
- PIR:   D70034
- RefSeq:   NP_391336.1
- ProteinModelPortal:   O06994
- SMR:   O06994
- EnsemblBacteria:   EBBACT00000001275
- GeneID:   938623
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU34560
- NMPDR:   fig|224308.1.peg.3462
- GenoList:   BSU34560
- GeneTree:   EBGT00070000031845
- HOGENOM:   HBG669028
- OMA:   AVYAMMN
- PhylomeDB:   O06994
- ProtClustDB:   CLSK237159
- BioCyc:   BSUB:BSU34560-MONOMER
- BRENDA:   3.2.1.10
- GO:   GO:0005737
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- SMART:   SM00642

Pfam domain/function: PF00128 Alpha-amylase; SSF51445 Glyco_hydro_cat

EC number: =3.2.1.10

Molecular weight: Translated: 66082; Mature: 65951

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: ACT_SITE 199-199 ACT_SITE 255-255

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEWWKEAVVYQIYPRSFYDANGDGFGDLQGVIQKLDYIKNLGADVIWLSPVFDSPQDDN
CCHHHHHCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
GYDISDYKNMYEKFGTNEDMFQLIDEVHKRGMKIVMDLVVNHTSDEHAWFAESRKSKDNP
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCC
YRDYYLWKDPKPDGSEPNNWGSIFSGSAWTYDEGTGQYYLHYFSKKQPDLNWENEAVRRE
CCCEEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCCEEEEEECCCCCCCCCCHHHHHHH
VYDVMRFWMDRGVDGWRMDVIGSISKYTDFPDYETDHSRSYIVGRYHSNGPRLHEFIQEM
HHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHH
NREVLSHYDCMTVGEANGSDIEEAKKYTDASRQELNMIFTFEHMDIDKEQNSPNGKWQIK
HHHHHHHCCCEEECCCCCCCHHHHHHHHCCCHHHEEEEEEEEECCCCCCCCCCCCEEEEC
PFDLIALKKTMTRWQTGLMNVGWNTLYFENHDQPRVISRWGNDRKLRKECAKAFATVLHG
CHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCC
MKGTPFIYQGEEIGMVNSDMPLEMYDDLEIKNAYRELVVENKTMSEKEFVKAVMIKGRDH
CCCCCEEECCCEECCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCC
ARTPMQWDAGKHAGFTAGDPWIPVNSRYQDINVKESLEDQDSIFFYYQKLIQLRKQYKIM
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCEEE
IYGDYQLLQENDPQVFSYLREYRGEKLLVVVNLSEEKALFEAPPELIHERWKVLISNYPQ
EEECCEEECCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHCCCHHHHHHHHHHHHHCCCH
ERADLKSISLKPYEAVMGISI
HHHHHHHCCCCHHHHHHCCCC
>Mature Secondary Structure 
SEWWKEAVVYQIYPRSFYDANGDGFGDLQGVIQKLDYIKNLGADVIWLSPVFDSPQDDN
CHHHHHCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
GYDISDYKNMYEKFGTNEDMFQLIDEVHKRGMKIVMDLVVNHTSDEHAWFAESRKSKDNP
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCC
YRDYYLWKDPKPDGSEPNNWGSIFSGSAWTYDEGTGQYYLHYFSKKQPDLNWENEAVRRE
CCCEEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCCEEEEEECCCCCCCCCCHHHHHHH
VYDVMRFWMDRGVDGWRMDVIGSISKYTDFPDYETDHSRSYIVGRYHSNGPRLHEFIQEM
HHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHH
NREVLSHYDCMTVGEANGSDIEEAKKYTDASRQELNMIFTFEHMDIDKEQNSPNGKWQIK
HHHHHHHCCCEEECCCCCCCHHHHHHHHCCCHHHEEEEEEEEECCCCCCCCCCCCEEEEC
PFDLIALKKTMTRWQTGLMNVGWNTLYFENHDQPRVISRWGNDRKLRKECAKAFATVLHG
CHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCC
MKGTPFIYQGEEIGMVNSDMPLEMYDDLEIKNAYRELVVENKTMSEKEFVKAVMIKGRDH
CCCCCEEECCCEECCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCC
ARTPMQWDAGKHAGFTAGDPWIPVNSRYQDINVKESLEDQDSIFFYYQKLIQLRKQYKIM
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCEEE
IYGDYQLLQENDPQVFSYLREYRGEKLLVVVNLSEEKALFEAPPELIHERWKVLISNYPQ
EEECCEEECCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHCCCHHHHHHHHHHHHHCCCH
ERADLKSISLKPYEAVMGISI
HHHHHHHCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 9573215; 10229946